Restriction map for
pFN21AB7371
(Circular) (Six-base) MAPSORT of: pFN21AB7371.seq Check: 7644 from: 1 to: 5653
pFN21AB7371 5653 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2741 2741
Size: 5653
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2240 2317 3716 5100 5368 1632
Size: 477 131 77 1399 1384 268 1917
Fragments arranged by size:
1917 1399 1384 477 268 131 77
AflII C'TTAA_G
Cuts at: 820 1017 2090 820
Size: 197 1073 4383
Fragments arranged by size:
4383 1073 197
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2932 3396 4968 2932
Size: 464 1572 3617
Fragments arranged by size:
3617 1572 464
ApaI G_GGCC'C
Cuts at: 2615 2615
Size: 5653
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2978 1870
Size: 73 87 948 4545
Fragments arranged by size:
4545 948 87 73
BamHI G'GATC_C
Cuts at: 2246 2983 3350 2246
Size: 737 367 4549
Fragments arranged by size:
4549 737 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2821 2974 4429 618
Size: 325 217 627 1034 153 1455 1842
Fragments arranged by size:
1842 1455 1034 627 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2388 2615 2972 1391
Size: 997 227 357 4072
Fragments arranged by size:
4072 997 357 227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 2269 928
Size: 971 216 154 4312
Fragments arranged by size:
4312 971 216 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2484 5648 2484
Size: 3164 2489
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2579 2615 3714 4870 1790
Size: 789 36 1099 1156 2573
Fragments arranged by size:
2573 1156 1099 789 36
BmgBI CAC'GTC
Cuts at: 1318 2164 1318
Size: 846 4807
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2468 2473 4313 1239
Size: 1133 96 5 1840 2579
Fragments arranged by size:
2579 1840 1133 96 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2509 1186
Size: 1323 4330
BseYI C'CCAG_C
Cuts at: 2626 4856 5352 2626
Size: 2230 496 2927
Fragments arranged by size:
2927 2230 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2452 2722 1163
Size: 573 716 270 4094
Fragments arranged by size:
4094 716 573 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3714 3799 4870 2031
Size: 1683 85 1071 2814
Fragments arranged by size:
2814 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2388 2579 2615 2972 3714 3799
Size: 399 241 357 191 36 357 742 85
Cuts at: 3799 4870 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 357 241 191
85 36
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2741 3008 844
Size: 1897 267 3489
Fragments arranged by size:
3489 1897 267
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2840 2942 4148 4322 65
Size: 1183 1592 102 1206 174 1396
Fragments arranged by size:
1592 1396 1206 1183 174 102
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2306 3713 4725 2306
Size: 1407 1012 3234
Fragments arranged by size:
3234 1407 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2932 794
Size: 2138 3515
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2246 2431 2484 2538 2869 2983 3350 3735
Size: 274 185 53 54 331 114 367 385
Cuts at: 3735 3752 5204 5290 5302 5648 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 331 274 185
114 86 54 53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2258 2281 513
Size: 552 939 254 23 3885
Fragments arranged by size:
3885 939 552 254 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 3169 3963 3983 3169
Size: 794 20 4839
Fragments arranged by size:
4839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2420 4660 809
Size: 507 1104 2240 1802
Fragments arranged by size:
2240 1802 1104 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618
Size: 54 1425 50 275 18 1270 889 1629
Cuts at: 5618 8
Size: 43
Fragments arranged by size:
1629 1425 1270 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2205 2699 2879 3583 1353
Size: 824 28 494 180 704 3423
Fragments arranged by size:
3423 824 704 494 180 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2240 2317 2372 2468 2473 3716
Size: 393 477 131 77 55 96 5 1243
Cuts at: 3716 4313 5100 5368 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1243 787 597 477 393 268 131
96 77 55 5
EcoO109I rG'GnC_Cy
Cuts at: 2215 2413 2611 2612 2754 2851 2215
Size: 198 198 1 142 97 5017
Fragments arranged by size:
5017 198 198 142 97 1
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2231 2263 2501
Size: 32 135 32 434 32 193 32 238
Cuts at: 2501 2533 4294 4326 4368 4400 1373
Size: 32 1761 32 42 32 2626
Fragments arranged by size:
2626 1761 434 238 193 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2546 4767 4951 1305
Size: 1241 2221 184 2007
Fragments arranged by size:
2221 2007 1241 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3611 3970 4129 518
Size: 648 1386 1059 359 159 2042
Fragments arranged by size:
2042 1386 1059 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3069 3746 4894 5139 1606
Size: 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2258 513
Size: 552 939 254 3908
Fragments arranged by size:
3908 939 552 254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2418 2450 3722 3754 5235 5267 2418
Size: 32 1272 32 1481 32 2804
Fragments arranged by size:
2804 1481 1272 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2754 2851 2413
Size: 341 97 5215
Fragments arranged by size:
5215 341 97
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2611 2611
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 3001 4136 4817 5008 826
Size: 219 1956 1135 681 191 1471
Fragments arranged by size:
1956 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 3757 4658 4920 820
Size: 197 926 147 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 2156 1517
Size: 639 5014
StyI C'CwwG_G
Cuts at: 513 1065 2004 2258 3092 513
Size: 552 939 254 834 3074
Fragments arranged by size:
3074 939 834 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 2312
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI