Restriction map for
pFN21AB7366
(Circular) (Six-base) MAPSORT of: pFN21AB7366.seq Check: 2 from: 1 to: 5440
pFN21AB7366 5440 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2297 2465 2297
Size: 168 5272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3503 4887 5155 1632
Size: 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 268
AfeI AGC'GCT
Cuts at: 2426 2426
Size: 5440
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 2035 2181 2224 3183 4755 2035
Size: 146 43 959 1572 2720
Fragments arranged by size:
2720 1572 959 146 43
ApaI G_GGCC'C
Cuts at: 2349 2524 2716 2349
Size: 175 192 5073
Fragments arranged by size:
5073 192 175
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2502 3994 160
Size: 872 1470 1492 1606
Fragments arranged by size:
1606 1492 1470 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2765 1870
Size: 73 207 615 4545
Fragments arranged by size:
4545 615 207 73
BamHI G'GATC_C
Cuts at: 2352 2770 3137 2352
Size: 418 367 4655
Fragments arranged by size:
4655 418 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 4216 618
Size: 325 217 627 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2349 2524 2716 2759 1391
Size: 958 175 192 43 4072
Fragments arranged by size:
4072 958 192 175 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4051 136
Size: 107 122 71 631 605 2379 1525
Fragments arranged by size:
2379 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2349 2403 2524 2716 3501 4657 1790
Size: 281 278 54 121 192 785 1156 2573
Fragments arranged by size:
2573 1156 785 281 278 192 121 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 3171 4129
Size: 416 53 83 399 24 852 902 958
Cuts at: 4129 442
Size: 1753
Fragments arranged by size:
1753 958 902 852 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 2602 4100 1239
Size: 1302 61 1498 2579
Fragments arranged by size:
2579 1498 1302 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3565 4430 4728 3565
Size: 865 298 4277
Fragments arranged by size:
4277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5215
Fragments arranged by size:
5215 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 1186
Size: 1501 3939
BseYI C'CCAG_C
Cuts at: 2070 2144 2708 4643 5139 2070
Size: 74 564 1935 496 2371
Fragments arranged by size:
2371 1935 564 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2185 2194 2210 1163
Size: 573 449 9 16 4393
Fragments arranged by size:
4393 573 449 16 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2517 2626 2890 3651 4679 1540
Size: 275 186 516 109 264 761 1028 2301
Fragments arranged by size:
2301 1028 761 516 275 264 186 109
BsiHKAI G_wGCw'C
Cuts at: 2333 3501 3586 4657 2333
Size: 1168 85 1071 3116
Fragments arranged by size:
3116 1168 1071 85
BsmI GAATG_Cn'
Cuts at: 2197 2388 2710 2951 3044 2197
Size: 191 322 241 93 4593
Fragments arranged by size:
4593 322 241 191 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2724 2724
Size: 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2333 2349 2403 2524 2716 2759
Size: 399 281 262 16 54 121 192 43
Cuts at: 2759 3501 3586 4657 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 281 262 192 121
85 54 43 16
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2297 2350 2465 2795 844
Size: 1265 188 53 115 330 3489
Fragments arranged by size:
3489 1265 330 188 115 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2622 3327 1394
Size: 429 799 705 3507
Fragments arranged by size:
3507 799 705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3935 4109 65
Size: 1183 2687 174 1396
Fragments arranged by size:
2687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 2247 96
Size: 2151 3289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 2721 3500 4512 2721
Size: 779 1012 3649
Fragments arranged by size:
3649 1012 779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2026 2035 794
Size: 1232 9 4199
Fragments arranged by size:
4199 1232 9
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2352 2770 3137 3522 3539 4991 5077 5089
Size: 380 418 367 385 17 1452 86 12
Cuts at: 5089 5435 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 418 385 380 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2494 2494
Size: 5440
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3750 3770 2956
Size: 794 20 4626
Fragments arranged by size:
4626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 2205 2514
Size: 54 1425 50 275 18 337 38 309
Cuts at: 2514 2623 2679 2697 2887 3776 5405 8
Size: 109 56 18 190 889 1629 43
Fragments arranged by size:
1629 1425 889 337 309 275 190 109
56 54 50 43 38 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2514 2623 2887 1537
Size: 275 702 109 264 4090
Fragments arranged by size:
4090 702 275 264 109
EarI CTCTTCn'nnn_
Cuts at: 1353 3370 1353
Size: 2017 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2541 2602 3503 4100 4887 5155 1239
Size: 393 909 61 901 597 787 268 1524
Fragments arranged by size:
1524 909 901 787 597 393 268 61
EcoO109I rG'GnC_Cy
Cuts at: 2345 2346 2520 2521 2712 2713 2345
Size: 1 174 1 191 1 5072
Fragments arranged by size:
5072 191 174 1 1 1
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 5440
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 2299 2428 4587 1678
Size: 621 129 2159 2531
Fragments arranged by size:
2531 2159 621 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 2784 3030 669
Size: 988 281 427 419 246 3079
Fragments arranged by size:
3079 988 427 419 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4554 4738 1305
Size: 3249 184 2007
Fragments arranged by size:
3249 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 2681 10
Size: 54 1425 343 337 512 2769
Fragments arranged by size:
2769 1425 512 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2636 3398 3757 3916 518
Size: 648 843 627 762 359 159 2042
Fragments arranged by size:
2042 843 762 648 627 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2316 2628 2856 3533 4681 4926 1606
Size: 426 284 312 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 426 312 284 245 228
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2623 2887 2623
Size: 264 5176
NspI r_CATG'y
Cuts at: 1132 1229 2798 4343 1132
Size: 97 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2656 1348
Size: 1308 4132
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PfoI T'CCnGG_A
Cuts at: 2307 2307
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2316 1613
Size: 703 4737
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2345 2520 2712 2345
Size: 175 192 5073
Fragments arranged by size:
5073 192 175
PstI C_TGCA'G
Cuts at: 830 2531 2792 830
Size: 1701 261 3478
Fragments arranged by size:
3478 1701 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2032 2316 2032
Size: 284 5156
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2527 2608 2788 3923 4604 4795 826
Size: 219 1482 81 180 1135 681 191 1471
Fragments arranged by size:
1482 1471 1135 681 219 191 180 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3544 4445 4707 820
Size: 197 926 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 2577 1517
Size: 1060 4380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2879 513
Size: 552 939 875 3074
Fragments arranged by size:
3074 939 875 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2350 3467 3484 3637 3822 4267 325
Size: 1255 770 1117 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1117 770 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 3686 5398
Size: 274 80 33 51 1443 270 1439 1712
Cuts at: 5398 96
Size: 138
Fragments arranged by size:
1712 1443 1439 274 270 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5400 5432 5400
Size: 32 5408
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2674 2674
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI