Restriction map for
pFN21AB7356
(Circular) (Six-base) MAPSORT of: pFN21AB7356.seq Check: 2984 from: 1 to: 8236
pFN21AB7356 8236 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4058 4058
Size: 8236
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6046 278
Size: 53 83 186 936 4510 2468
Fragments arranged by size:
4510 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5579 1937
Size: 3642 4594
Acc65I G'GTAC_C
Cuts at: 5557 5557
Size: 8236
AclI AA'CG_TT
Cuts at: 1563 6363 6736 1563
Size: 4800 373 3063
Fragments arranged by size:
4800 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3218 3305 4246 4556 6299 7683 7951
Size: 413 1173 87 941 310 1743 1384 268
Cuts at: 7951 1632
Size: 1917
Fragments arranged by size:
1917 1743 1384 1173 941 413 310 268
87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8039
AflIII A'CryG_T
Cuts at: 1203 2141 4247 7135 8112 1203
Size: 938 2106 2888 977 1327
Fragments arranged by size:
2888 2106 1327 977 938
AgeI A'CCGG_T
Cuts at: 7067 7067
Size: 8236
AhdI GACnn_n'nnGTC
Cuts at: 6965 6965
Size: 8236
AleI CACnn'nnGTG
Cuts at: 1166 4250 1166
Size: 3084 5152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3419 1184
Size: 2235 6001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 2745
Size: 32 8204
AlwNI CAG_nnn'CTG
Cuts at: 2017 2269 3196 3691 3708 4951 5979 7551 2017
Size: 252 927 495 17 1243 1028 1572 2702
Fragments arranged by size:
2702 1572 1243 1028 927 495 252 17
ApaI G_GGCC'C
Cuts at: 3320 4885 3320
Size: 1565 6671
ApaLI G'TGCA_C
Cuts at: 5147 6293 7449 5147
Size: 1146 1156 5934
Fragments arranged by size:
5934 1156 1146
ApoI r'AATT_y
Cuts at: 1493 2375 2791 5208 5545 5771 5923 8032 1493
Size: 882 416 2417 337 226 152 2109 1697
Fragments arranged by size:
2417 2109 1697 882 416 337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4590 6790 160
Size: 872 3558 2200 1606
Fragments arranged by size:
3558 2200 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
AvaI C'yCGr_G
Cuts at: 1870 1943 5561 1870
Size: 73 3618 4545
Fragments arranged by size:
4545 3618 73
BamHI G'GATC_C
Cuts at: 5566 5933 5566
Size: 367 7869
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5557 7012 618
Size: 325 217 627 3770 1455 1842
Fragments arranged by size:
3770 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3320 3594 4885 4932 5426 5477 5555 1391
Size: 1929 274 1291 47 494 51 78 4072
Fragments arranged by size:
4072 1929 1291 494 274 78 51 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 4878 928
Size: 971 345 2634 4286
Fragments arranged by size:
4286 2634 971 345
BbvCI CC'TCA_GC
Cuts at: 3668 3668
Size: 8236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6427 6461 1903
Size: 34 50 34 4406 34 3678
Fragments arranged by size:
4406 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4640 6128 7338 4640
Size: 1488 1210 5538
Fragments arranged by size:
5538 1488 1210
BclI T'GATC_A
Cuts at: 1548 3909 4315 1548
Size: 2361 406 5469
Fragments arranged by size:
5469 2361 406
BfrBI ATG'CAT
Cuts at: 4120 4120
Size: 8236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6847 136
Size: 107 122 71 631 605 5175 1525
Fragments arranged by size:
5175 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8231 8231
Size: 8236
BlpI GC'TnA_GC
Cuts at: 5653 5653
Size: 8236
Bme1580I G_kGCm'C
Cuts at: 1790 3320 3500 4885 5151 6297 7453 1790
Size: 1530 180 1385 266 1146 1156 2573
Fragments arranged by size:
2573 1530 1385 1156 1146 266 180
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8236
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5967 6925 442
Size: 416 53 83 399 24 4550 958 1753
Fragments arranged by size:
4550 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1994 3164 3541 5379 6896 1239
Size: 755 1170 377 1838 1517 2579
Fragments arranged by size:
2579 1838 1517 1170 755 377
Bpu10I CC'TnA_GC
Cuts at: 3668 5306 3668
Size: 1638 6598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5331 6361 7226 7524 5331
Size: 1030 865 298 6043
Fragments arranged by size:
6043 1030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6899 882
Size: 617 5400 2219
Fragments arranged by size:
5400 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2142 4248 6027 493
Size: 711 938 2106 1779 2702
Fragments arranged by size:
2702 2106 1779 938 711
BsaBI GATnn'nnATC
Cuts at: 8230 8230
Size: 8236
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4416 4443 4875
Size: 53 83 186 936 2764 119 27 432
Cuts at: 4875 5390 6043 6425 7060 275
Size: 515 653 382 635 1451
Fragments arranged by size:
2764 1451 936 653 635 515 432 382
186 119 83 53 27
BsaWI w'CCGG_w
Cuts at: 3228 6669 7067 7341 7488 7899 3228
Size: 3441 398 274 147 411 3565
Fragments arranged by size:
3565 3441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 1178
Size: 30 165 30 798 30 7183
Fragments arranged by size:
7183 798 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 5384 1186
Size: 1036 3162 4038
Fragments arranged by size:
4038 3162 1036
BseYI C'CCAG_C
Cuts at: 3320 5427 7439 7935 3320
Size: 2107 2012 496 3621
Fragments arranged by size:
3621 2107 2012 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2245 2374 3804 5466 1163
Size: 573 509 129 1430 1662 3933
Fragments arranged by size:
3933 1662 1430 573 509 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5686 6447 7475 1540
Size: 275 186 3685 761 1028 2301
Fragments arranged by size:
3685 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3228 5151 5477 6297 6382 7453 3228
Size: 1923 326 820 85 1071 4011
Fragments arranged by size:
4011 1923 1071 820 326 85
BsmI GAATG_Cn'
Cuts at: 4684 5747 5840 4684
Size: 1063 93 7080
Fragments arranged by size:
7080 1063 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3228 3320 3500 3594 4885 4932 5151
Size: 399 1438 92 180 94 1291 47 219
Cuts at: 5151 5426 5477 5555 6297 6382 7453 1391
Size: 275 51 78 742 85 1071 2174
Fragments arranged by size:
2174 1438 1291 1071 742 399 275 219
180 94 92 85 78 51 47
BspEI T'CCGG_A
Cuts at: 3228 7899 3228
Size: 4671 3565
BspHI T'CATG_A
Cuts at: 2086 3888 6125 7855 2086
Size: 1802 2237 1730 2467
Fragments arranged by size:
2467 2237 1802 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2455 4058 5029 5591 844
Size: 1611 1603 971 562 3489
Fragments arranged by size:
3489 1611 1603 971 562
BsrBI CCG'CTC
Cuts at: 1394 1823 4439 6123 1394
Size: 429 2616 1684 3507
Fragments arranged by size:
3507 2616 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2938 3949 6731 6905 65
Size: 1183 1690 1011 2782 174 1396
Fragments arranged by size:
2782 1690 1396 1183 1011 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6880 7067 1700
Size: 110 5070 187 2869
Fragments arranged by size:
5070 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8236
BssSI C'ACGA_G
Cuts at: 6296 7308 6296
Size: 1012 7224
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5405 794
Size: 4611 3625
BstBI TT'CG_AA
Cuts at: 7040 7040
Size: 8236
BstEII G'GTnAC_C
Cuts at: 1184 2840 1184
Size: 1656 6580
BstXI CCAn_nnnn'nTGG
Cuts at: 5224 5492 5224
Size: 268 7968
BstYI r'GATC_y
Cuts at: 1972 2035 4566 4720 5566 5933 6318 6335 7787
Size: 63 2531 154 846 367 385 17 1452
Cuts at: 7787 7873 7885 8231 1972
Size: 86 12 346 1977
Fragments arranged by size:
2531 1977 1452 846 385 367 346 154
86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2846 3701 5433 2846
Size: 855 1732 5649
Fragments arranged by size:
5649 1732 855
BtgI C'CryG_G
Cuts at: 513 1065 3216 3918 5217 513
Size: 552 2151 702 1299 3532
Fragments arranged by size:
3532 2151 1299 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7497
Fragments arranged by size:
7497 683 56
BtsI GCAGTG_nn'
Cuts at: 5752 6546 6566 5752
Size: 794 20 7422
Fragments arranged by size:
7422 794 20
ClaI AT'CG_AT
Cuts at: 1554 4911 1554
Size: 3357 4879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8201
DraI TTT'AAA
Cuts at: 2952 3141 3787 5172 5540 2952
Size: 189 646 1385 368 5648
Fragments arranged by size:
5648 1385 646 368 189
DraIII CAC_nnn'GTG
Cuts at: 2786 2786
Size: 8236
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4217 4448 7243 809
Size: 507 2901 231 2795 1802
Fragments arranged by size:
2901 2795 1802 507 231
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2735 2990 4230
Size: 54 1425 50 275 18 905 255 1240
Cuts at: 4230 4696 5683 6572 8201 8
Size: 466 987 889 1629 43
Fragments arranged by size:
1629 1425 1240 987 905 889 466 275
255 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5683 1537
Size: 275 3871 4090
Fragments arranged by size:
4090 3871 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3300 3570 3918 6166 1353
Size: 1947 270 348 2248 3423
Fragments arranged by size:
3423 2248 1947 348 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6823 7197 7343 387
Size: 1064 138 5234 374 146 1280
Fragments arranged by size:
5234 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5475 5475
Size: 8236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1994 2045 3164 3218 3305 3541 4246
Size: 393 362 51 1119 54 87 236 705
Cuts at: 4246 4556 5379 6299 6896 7683 7951 1239
Size: 310 823 920 597 787 268 1524
Fragments arranged by size:
1524 1119 920 823 787 705 597 393
362 310 268 236 87 54 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3287 3725 3287
Size: 438 7798
EcoO109I rG'GnC_Cy
Cuts at: 3202 3317 4450 4882 3202
Size: 115 1133 432 6556
Fragments arranged by size:
6556 1133 432 115
EcoRI G'AATT_C
Cuts at: 5545 5923 5545
Size: 378 7858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3265 3417 3265
Size: 152 8084
FspI TGC'GCA
Cuts at: 6742 6742
Size: 8236
HaeII r_GCGC'y
Cuts at: 1678 7383 1678
Size: 5705 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 3155 3187 5348
Size: 32 135 32 448 32 1103 32 2161
Cuts at: 5348 5380 6877 6909 6951 6983 1373
Size: 32 1497 32 42 32 2626
Fragments arranged by size:
2626 2161 1497 1103 448 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3426 5580 5826 669
Size: 988 281 1488 2154 246 3079
Fragments arranged by size:
3079 2154 1488 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3620 748
Size: 2872 5364
HpaI GTT'AAC
Cuts at: 5826 5826
Size: 8236
KpnI G_GTAC'C
Cuts at: 5561 5561
Size: 8236
MfeI C'AATT_G
Cuts at: 3585 5835 3585
Size: 2250 5986
MluI A'CGCG_T
Cuts at: 8112 8112
Size: 8236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3823 3973 4833 5062 7350 7534 1305
Size: 2518 150 860 229 2288 184 2007
Fragments arranged by size:
2518 2288 2007 860 229 184 150
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2737 2992 4232 10
Size: 54 1425 343 905 255 1240 4014
Fragments arranged by size:
4014 1425 1240 905 343 255 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3917 4250 5222 6194 6553 6712 518
Size: 648 2751 333 972 972 359 159 2042
Fragments arranged by size:
2751 2042 972 972 648 359 333 159
MspA1I CmG'CkG
Cuts at: 1606 3271 4948 5652 6329 7477 7722 1606
Size: 1665 1677 704 677 1148 245 2120
Fragments arranged by size:
2120 1677 1665 1148 704 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3918 5217 513
Size: 552 2853 1299 3532
Fragments arranged by size:
3532 2853 1299 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8236
NotI GC'GGCC_GC
Cuts at: 5683 5683
Size: 8236
NsiI A_TGCA'T
Cuts at: 4122 4122
Size: 8236
NspI r_CATG'y
Cuts at: 1132 1229 3553 4312 5594 7139 1132
Size: 97 2324 759 1282 1545 2229
Fragments arranged by size:
2324 2229 1545 1282 759 97
PasI CC'CwG_GG
Cuts at: 1348 3500 1348
Size: 2152 6084
PciI A'CATG_T
Cuts at: 7135 7135
Size: 8236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2017 2269 2457 4411 1108
Size: 909 252 188 1954 4933
Fragments arranged by size:
4933 1954 909 252 188
PfoI T'CCnGG_A
Cuts at: 3720 3720
Size: 8236
PmeI GTTT'AAAC
Cuts at: 5540 5540
Size: 8236
PmlI CAC'GTG
Cuts at: 2142 4248 2142
Size: 2106 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6305 6337 7818 7850 6305
Size: 32 1481 32 6691
Fragments arranged by size:
6691 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4450 4450
Size: 8236
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8236
PsiI TTA'TAA
Cuts at: 2060 5193 5806 2060
Size: 3133 613 4490
Fragments arranged by size:
4490 3133 613
PspOMI G'GGCC_C
Cuts at: 3316 4881 3316
Size: 1565 6671
PstI C_TGCA'G
Cuts at: 830 3135 3210 4348 5421 5588 830
Size: 2305 75 1138 1073 167 3478
Fragments arranged by size:
3478 2305 1138 1073 167 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8236
PvuII CAG'CTG
Cuts at: 4948 4948
Size: 8236
SacI G_AGCT'C
Cuts at: 5477 5477
Size: 8236
SalI G'TCGA_C
Cuts at: 1936 5578 1936
Size: 3642 4594
SapI GCTCTTCn'nnn_
Cuts at: 3300 3300
Size: 8236
SbfI CC_TGCA'GG
Cuts at: 5588 5588
Size: 8236
ScaI AGT'ACT
Cuts at: 2434 2480 6484 8196 2434
Size: 46 4004 1712 2474
Fragments arranged by size:
4004 2474 1712 46
SfcI C'TryA_G
Cuts at: 826 1045 2362 3131 3206 4056 4344 5008 5417
Size: 219 1317 769 75 850 288 664 409
Cuts at: 5417 5584 6719 7400 7591 826
Size: 167 1135 681 191 1471
Fragments arranged by size:
1471 1317 1135 850 769 681 664 409
288 219 191 167 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8236
SmaI CCC'GGG
Cuts at: 1872 5563 1872
Size: 3691 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5346 6340 7241 7503 820
Size: 197 926 3403 994 901 262 1553
Fragments arranged by size:
3403 1553 994 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6027 493
Size: 5534 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8236
SphI G_CATG'C
Cuts at: 1132 5594 1132
Size: 4462 3774
SspI AAT'ATT
Cuts at: 5 52 3796 4104 6160 5
Size: 47 3744 308 2056 2081
Fragments arranged by size:
3744 2081 2056 308 47
StuI AGG'CCT
Cuts at: 1517 3562 4519 1517
Size: 2045 957 5234
Fragments arranged by size:
5234 2045 957
StyI C'CwwG_G
Cuts at: 513 1065 3626 3918 4446 5217 5675 513
Size: 552 2561 292 528 771 458 3074
Fragments arranged by size:
3074 2561 771 552 528 458 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3492 4593 6263 6280 6433 6618 7063
Size: 1255 1912 1101 1670 17 153 185 445
Cuts at: 7063 325
Size: 1498
Fragments arranged by size:
1912 1670 1498 1255 1101 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 2432 2478
Size: 274 80 33 51 1443 408 47 46
Cuts at: 2478 2985 3958 6482 8194 96
Size: 507 973 2524 1712 138
Fragments arranged by size:
2524 1712 1443 973 507 408 274 138
80 51 47 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 8196 8228 2201
Size: 32 5963 32 2209
Fragments arranged by size:
5963 2209 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4217 1316
Size: 2901 5335
XbaI T'CTAG_A
Cuts at: 5572 5572
Size: 8236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2046 5492 2046
Size: 3446 4790
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8236
XmaI C'CCGG_G
Cuts at: 1870 5561 1870
Size: 3691 4545
XmnI GAAnn'nnTTC
Cuts at: 2126 6365 2126
Size: 4239 3997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6044 276
Size: 53 83 186 936 4510 2468
Fragments arranged by size:
4510 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI