Restriction map for
pFN21AB7355
(Circular) (Six-base) MAPSORT of: pFN21AB7355.seq Check: 3186 from: 1 to: 7969
pFN21AB7355 7969 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 5779 278
Size: 53 83 186 936 1942 2301 2468
Fragments arranged by size:
2468 2301 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3492 5312 1937
Size: 1555 1820 4594
Fragments arranged by size:
4594 1820 1555
Acc65I G'GTAC_C
Cuts at: 5290 5290
Size: 7969
AclI AA'CG_TT
Cuts at: 1563 6096 6469 1563
Size: 4533 373 3063
Fragments arranged by size:
4533 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2374 2496 2606 3081 3827 3970 4244 4378
Size: 742 122 110 475 746 143 274 134
Cuts at: 4378 5104 6032 7416 7684 1632
Size: 726 928 1384 268 1917
Fragments arranged by size:
1917 1384 928 746 742 726 475 274
268 143 134 122 110
AfeI AGC'GCT
Cuts at: 4014 4014
Size: 7969
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7772
AflIII A'CryG_T
Cuts at: 1203 2198 3371 4295 6868 7845 1203
Size: 995 1173 924 2573 977 1327
Fragments arranged by size:
2573 1327 1173 995 977 924
AgeI A'CCGG_T
Cuts at: 6800 6800
Size: 7969
AhdI GACnn_n'nnGTC
Cuts at: 6698 6698
Size: 7969
AleI CACnn'nnGTG
Cuts at: 1166 3933 1166
Size: 2767 5202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7969
AlwNI CAG_nnn'CTG
Cuts at: 4438 4803 5152 5712 7284 4438
Size: 365 349 560 1572 5123
Fragments arranged by size:
5123 1572 560 365 349
ApaLI G'TGCA_C
Cuts at: 3340 6026 7182 3340
Size: 2686 1156 4127
Fragments arranged by size:
4127 2686 1156
ApoI r'AATT_y
Cuts at: 1493 2987 3113 3911 4058 4066 4357 4383 5278
Size: 1494 126 798 147 8 291 26 895
Cuts at: 5278 5504 5656 7765 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1494 895 798 291 226 152
147 126 26 8
AseI AT'TA_AT
Cuts at: 160 1032 6523 160
Size: 872 5491 1606
Fragments arranged by size:
5491 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7969
AvaI C'yCGr_G
Cuts at: 1870 1943 5294 1870
Size: 73 3351 4545
Fragments arranged by size:
4545 3351 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4773 4806 4773
Size: 33 7936
BamHI G'GATC_C
Cuts at: 5299 5666 5299
Size: 367 7602
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5290 6745 618
Size: 325 217 627 3503 1455 1842
Fragments arranged by size:
3503 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5028 5288 1391
Size: 3637 260 4072
Fragments arranged by size:
4072 3637 260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 4546 4989 928
Size: 971 481 2166 443 3908
Fragments arranged by size:
3908 2166 971 481 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3386 3420 6160 6194 1903
Size: 34 1449 34 2740 34 3678
Fragments arranged by size:
3678 2740 1449 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3598 5017 5861 7071 2934
Size: 664 1419 844 1210 3832
Fragments arranged by size:
3832 1419 1210 844 664
BclI T'GATC_A
Cuts at: 1548 3691 1548
Size: 2143 5826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6580 136
Size: 107 122 71 631 605 4908 1525
Fragments arranged by size:
4908 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4036 4171 7964 4036
Size: 135 3793 4041
Fragments arranged by size:
4041 3793 135
BlpI GC'TnA_GC
Cuts at: 2181 5386 2181
Size: 3205 4764
Bme1580I G_kGCm'C
Cuts at: 1790 3344 6030 7186 1790
Size: 1554 2686 1156 2573
Fragments arranged by size:
2686 2573 1554 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7969
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3118 3817 5107
Size: 416 53 83 399 24 1701 699 1290
Cuts at: 5107 5700 6658 442
Size: 593 958 1753
Fragments arranged by size:
1753 1701 1290 958 699 593 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2579 1056
Size: 1523 6446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 4577 4780 6629 1239
Size: 1169 2169 203 1849 2579
Fragments arranged by size:
2579 2169 1849 1169 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4469 4499 6094 6959 7257 4469
Size: 30 1595 865 298 5181
Fragments arranged by size:
5181 1595 865 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6632 882
Size: 617 5133 2219
Fragments arranged by size:
5133 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2281 5760 493
Size: 711 1077 3479 2702
Fragments arranged by size:
3479 2702 1077 711
BsaBI GATnn'nnATC
Cuts at: 2255 7963 2255
Size: 5708 2261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 4551 5776 6158
Size: 53 83 186 936 1942 1076 1225 382
Cuts at: 6158 6793 275
Size: 635 1451
Fragments arranged by size:
1942 1451 1225 1076 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3608 4647 6402 6800 7074 7221 7632 3608
Size: 1039 1755 398 274 147 411 3945
Fragments arranged by size:
3945 1755 1039 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3024 3054 3323 3353 1178
Size: 30 165 30 1621 30 269 30 5794
Fragments arranged by size:
5794 1621 269 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4783 1186
Size: 3597 4372
BseYI C'CCAG_C
Cuts at: 3028 4347 7172 7668 3028
Size: 1319 2825 496 3329
Fragments arranged by size:
3329 2825 1319 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1997 2759 3407 1163
Size: 573 261 762 648 5725
Fragments arranged by size:
5725 762 648 573 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5419 6180 7208 1540
Size: 275 186 3418 761 1028 2301
Fragments arranged by size:
3418 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3344 3379 6030 6115 7186 3344
Size: 35 2651 85 1071 4127
Fragments arranged by size:
4127 2651 1071 85 35
BsmI GAATG_Cn'
Cuts at: 4270 5480 5573 4270
Size: 1210 93 6666
Fragments arranged by size:
6666 1210 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3764 3764
Size: 7969
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3344 3379 5028 5288 6030 6115 7186
Size: 399 1554 35 1649 260 742 85 1071
Cuts at: 7186 1391
Size: 2174
Fragments arranged by size:
2174 1649 1554 1071 742 399 260 85
35
BspEI T'CCGG_A
Cuts at: 7632 7632
Size: 7969
BspHI T'CATG_A
Cuts at: 2983 3777 4962 5858 7588 2983
Size: 794 1185 896 1730 3364
Fragments arranged by size:
3364 1730 1185 896 794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 5324 844
Size: 1800 2680 3489
Fragments arranged by size:
3489 2680 1800
BsrBI CCG'CTC
Cuts at: 1394 1823 5856 1394
Size: 429 4033 3507
Fragments arranged by size:
4033 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 6464 6638 65
Size: 1183 1086 4130 174 1396
Fragments arranged by size:
4130 1396 1183 1086 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6613 6800 1700
Size: 110 4803 187 2869
Fragments arranged by size:
4803 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4298 96
Size: 4202 3767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7969
BssSI C'ACGA_G
Cuts at: 3931 6029 7041 3931
Size: 2098 1012 4859
Fragments arranged by size:
4859 2098 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7969
BstBI TT'CG_AA
Cuts at: 6773 6773
Size: 7969
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7969
BstXI CCAn_nnnn'nTGG
Cuts at: 2754 2754
Size: 7969
BstYI r'GATC_y
Cuts at: 1972 2788 4036 4171 4343 4434 5299 5666 6051
Size: 816 1248 135 172 91 865 367 385
Cuts at: 6051 6068 7520 7606 7618 7964 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1248 865 816 385 367 346
172 135 91 86 17 12
BstZ17I GTA'TAC
Cuts at: 3493 3493
Size: 7969
Bsu36I CC'TnA_GG
Cuts at: 2955 2955
Size: 7969
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6478
Fragments arranged by size:
6478 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7230
Fragments arranged by size:
7230 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 5233 5485 6279 6299 2440
Size: 2793 252 794 20 4110
Fragments arranged by size:
4110 2793 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7934
DraI TTT'AAA
Cuts at: 2592 2616 3012 3882 4129 5273 2592
Size: 24 396 870 247 1144 5288
Fragments arranged by size:
5288 1144 870 396 247 24
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2384 2875 4823 6976 809
Size: 507 1068 491 1948 2153 1802
Fragments arranged by size:
2153 1948 1802 1068 507 491
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5416 6305 7934
Size: 54 1425 50 275 18 3586 889 1629
Cuts at: 7934 8
Size: 43
Fragments arranged by size:
3586 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5416 1537
Size: 275 3604 4090
Fragments arranged by size:
4090 3604 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3384 3846 4397 4841 5197 5899 1353
Size: 2031 462 551 444 356 702 3423
Fragments arranged by size:
3423 2031 702 551 462 444 356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6556 6930 7076 387
Size: 1064 138 4967 374 146 1280
Fragments arranged by size:
4967 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2408 2496 2606 3081 3827 3970
Size: 393 742 34 88 110 475 746 143
Cuts at: 3970 4244 4378 4577 4780 5104 6032 6629 7416
Size: 274 134 199 203 324 928 597 787
Cuts at: 7416 7684 1239
Size: 268 1524
Fragments arranged by size:
1524 928 787 746 742 597 475 393
324 274 268 203 199 143 134 110
88 34
EcoO109I rG'GnC_Cy
Cuts at: 2877 5104 2877
Size: 2227 5742
EcoRI G'AATT_C
Cuts at: 4383 5278 5656 4383
Size: 895 378 6696
Fragments arranged by size:
6696 895 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3494 4540 3494
Size: 1046 6923
FspI TGC'GCA
Cuts at: 5225 6475 5225
Size: 1250 6719
HaeII r_GCGC'y
Cuts at: 1678 2012 4016 7116 1678
Size: 334 2004 3100 2531
Fragments arranged by size:
3100 2531 2004 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2445 2477 3007 3039 3361
Size: 32 135 32 873 32 530 32 322
Cuts at: 3361 3393 4021 4053 4355 4387 6610 6642 6684
Size: 32 628 32 302 32 2223 32 42
Cuts at: 6684 6716 1373
Size: 32 2626
Fragments arranged by size:
2626 2223 873 628 530 322 302 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2147 5313 5559 669
Size: 988 281 209 3166 246 3079
Fragments arranged by size:
3166 3079 988 281 246 209
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7969
HpaI GTT'AAC
Cuts at: 5559 5559
Size: 7969
KpnI G_GTAC'C
Cuts at: 5294 5294
Size: 7969
MfeI C'AATT_G
Cuts at: 5568 5568
Size: 7969
MluI A'CGCG_T
Cuts at: 7845 7845
Size: 7969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2771 3234 3309 4970 7083 7267 1305
Size: 1466 463 75 1661 2113 184 2007
Fragments arranged by size:
2113 2007 1661 1466 463 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6147
Fragments arranged by size:
6147 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2845 3272 3288 3933 4873 5927 6286
Size: 648 1679 427 16 645 940 1054 359
Cuts at: 6286 6445 518
Size: 159 2042
Fragments arranged by size:
2042 1679 1054 940 648 645 427 359
159 16
MspA1I CmG'CkG
Cuts at: 1606 3028 4800 5385 6062 7210 7455 1606
Size: 1422 1772 585 677 1148 245 2120
Fragments arranged by size:
2120 1772 1422 1148 677 585 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6478
Fragments arranged by size:
6478 939 552
NdeI CA'TA_TG
Cuts at: 387 3682 4701 387
Size: 3295 1019 3655
Fragments arranged by size:
3655 3295 1019
NheI G'CTAG_C
Cuts at: 1052 2575 1052
Size: 1523 6446
NotI GC'GGCC_GC
Cuts at: 5416 5416
Size: 7969
NspI r_CATG'y
Cuts at: 1132 1229 2202 2583 3375 4299 5327 6872 1132
Size: 97 973 381 792 924 1028 1545 2229
Fragments arranged by size:
2229 1545 1028 973 924 792 381 97
PacI TTA_AT'TAA
Cuts at: 2925 2925
Size: 7969
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7969
PciI A'CATG_T
Cuts at: 2198 3371 4295 6868 2198
Size: 1173 924 2573 3299
Fragments arranged by size:
3299 2573 1173 924
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2026 3440 1108
Size: 918 1414 5637
Fragments arranged by size:
5637 1414 918
PfoI T'CCnGG_A
Cuts at: 2769 4677 2769
Size: 1908 6061
PmeI GTTT'AAAC
Cuts at: 5273 5273
Size: 7969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6038 6070 7551 7583 6038
Size: 32 1481 32 6424
Fragments arranged by size:
6424 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2877 5104 2877
Size: 2227 5742
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7969
PsiI TTA'TAA
Cuts at: 5539 5539
Size: 7969
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3156 3188 3156
Size: 32 7937
PstI C_TGCA'G
Cuts at: 830 2722 4944 5321 830
Size: 1892 2222 377 3478
Fragments arranged by size:
3478 2222 1892 377
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7969
PvuII CAG'CTG
Cuts at: 3028 4800 3028
Size: 1772 6197
SalI G'TCGA_C
Cuts at: 1936 5311 1936
Size: 3375 4594
SapI GCTCTTCn'nnn_
Cuts at: 3384 3384
Size: 7969
SbfI CC_TGCA'GG
Cuts at: 5321 5321
Size: 7969
ScaI AGT'ACT
Cuts at: 6217 7929 6217
Size: 1712 6257
SexAI A'CCwGG_T
Cuts at: 2490 2490
Size: 7969
SfcI C'TryA_G
Cuts at: 826 1045 2718 3101 4940 5317 6452 7133 7324
Size: 219 1673 383 1839 377 1135 681 191
Cuts at: 7324 826
Size: 1471
Fragments arranged by size:
1839 1673 1471 1135 681 383 377 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7969
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7969
SmaI CCC'GGG
Cuts at: 1872 5296 1872
Size: 3424 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4484 4514 6073 6974 7236 820
Size: 197 926 2541 30 1559 901 262 1553
Fragments arranged by size:
2541 1559 1553 926 901 262 197 30
SnaBI TAC'GTA
Cuts at: 493 5760 493
Size: 5267 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7969
SphI G_CATG'C
Cuts at: 1132 2583 5327 1132
Size: 1451 2744 3774
Fragments arranged by size:
3774 2744 1451
SspI AAT'ATT
Cuts at: 5 52 4113 5893 5
Size: 47 4061 1780 2081
Fragments arranged by size:
4061 2081 1780 47
StuI AGG'CCT
Cuts at: 1517 3236 5014 1517
Size: 1719 1778 4472
Fragments arranged by size:
4472 1778 1719
StyI C'CwwG_G
Cuts at: 513 1065 2004 2911 4444 5408 513
Size: 552 939 907 1533 964 3074
Fragments arranged by size:
3074 1533 964 939 907 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 5195 5996 6013 6166 6351 6796
Size: 1255 2451 1164 801 17 153 185 445
Cuts at: 6796 325
Size: 1498
Fragments arranged by size:
2451 1498 1255 1164 801 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2048 3536 4298
Size: 274 80 33 51 1443 71 1488 762
Cuts at: 4298 4588 4732 6215 7927 96
Size: 290 144 1483 1712 138
Fragments arranged by size:
1712 1488 1483 1443 762 290 274 144
138 80 71 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 7929 7961 3323
Size: 32 4574 32 3331
Fragments arranged by size:
4574 3331 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7969
XbaI T'CTAG_A
Cuts at: 2504 3766 5305 2504
Size: 1262 1539 5168
Fragments arranged by size:
5168 1539 1262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5017 5017
Size: 7969
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7969
XmaI C'CCGG_G
Cuts at: 1870 5294 1870
Size: 3424 4545
XmnI GAAnn'nnTTC
Cuts at: 5048 6098 5048
Size: 1050 6919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 5777 276
Size: 53 83 186 936 1942 2301 2468
Fragments arranged by size:
2468 2301 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI
PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI