Restriction map for
pFN21AB7354
(Circular) (Six-base) MAPSORT of: pFN21AB7354.seq Check: 3679 from: 1 to: 8236
pFN21AB7354 8236 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6046 278
Size: 53 83 186 936 4510 2468
Fragments arranged by size:
4510 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 2930 3307 4809 5579 1937
Size: 847 146 377 1502 770 4594
Fragments arranged by size:
4594 1502 847 770 377 146
Acc65I G'GTAC_C
Cuts at: 5557 5557
Size: 8236
AclI AA'CG_TT
Cuts at: 1563 6363 6736 1563
Size: 4800 373 3063
Fragments arranged by size:
4800 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2922 2964 3324 3672 3747 4894 6299
Size: 941 349 42 360 348 75 1147 1405
Cuts at: 6299 7683 7951 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1405 1384 1147 941 360 349 348
268 75 42
AflII C'TTAA_G
Cuts at: 820 1017 4870 820
Size: 197 3853 4186
Fragments arranged by size:
4186 3853 197
AflIII A'CryG_T
Cuts at: 1203 2560 3213 5249 7135 8112 1203
Size: 1357 653 2036 1886 977 1327
Fragments arranged by size:
2036 1886 1357 1327 977 653
AgeI A'CCGG_T
Cuts at: 7067 7067
Size: 8236
AhdI GACnn_n'nnGTC
Cuts at: 6965 6965
Size: 8236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8236
AlwNI CAG_nnn'CTG
Cuts at: 2029 2306 3486 5203 5979 7551 2029
Size: 277 1180 1717 776 1572 2714
Fragments arranged by size:
2714 1717 1572 1180 776 277
ApaLI G'TGCA_C
Cuts at: 6293 7449 6293
Size: 1156 7080
ApoI r'AATT_y
Cuts at: 1493 2117 2161 2176 2460 2766 3098 3716 3846
Size: 624 44 15 284 306 332 618 130
Cuts at: 3846 3860 3905 5545 5771 5923 8032 1493
Size: 14 45 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 624 618 332 306 284
226 152 130 45 44 15 14
AseI AT'TA_AT
Cuts at: 160 1032 3276 3300 6790 160
Size: 872 2244 24 3490 1606
Fragments arranged by size:
3490 2244 1606 872 24
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
AvaI C'yCGr_G
Cuts at: 1870 1943 5561 1870
Size: 73 3618 4545
Fragments arranged by size:
4545 3618 73
BamHI G'GATC_C
Cuts at: 2016 4937 5473 5566 5933 2016
Size: 2921 536 93 367 4319
Fragments arranged by size:
4319 2921 536 367 93
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3556 3757 5557 7012 618
Size: 325 217 627 1769 201 1800 1455 1842
Fragments arranged by size:
1842 1800 1769 1455 627 325 217 201
BanII G_rGCy'C
Cuts at: 1391 2371 5171 5555 1391
Size: 980 2800 384 4072
Fragments arranged by size:
4072 2800 980 384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 4053 4540 5011 928
Size: 971 662 1492 487 471 4153
Fragments arranged by size:
4153 1492 971 662 487 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6427 6461 1903
Size: 34 4490 34 3678
Fragments arranged by size:
4490 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6128 7338 6128
Size: 1210 7026
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 7108
BfrBI ATG'CAT
Cuts at: 3581 3581
Size: 8236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6847 136
Size: 107 122 71 631 605 5175 1525
Fragments arranged by size:
5175 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8231 8231
Size: 8236
BlpI GC'TnA_GC
Cuts at: 5653 5653
Size: 8236
Bme1580I G_kGCm'C
Cuts at: 1790 6297 7453 1790
Size: 4507 1156 2573
Fragments arranged by size:
4507 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8236
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4131 4675 5967
Size: 416 53 83 399 24 2714 544 1292
Cuts at: 5967 6925 442
Size: 958 1753
Fragments arranged by size:
2714 1753 1292 958 544 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5168 5168
Size: 8236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6896 1239
Size: 5657 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3150 6361 7226 7524 3150
Size: 3211 865 298 3862
Fragments arranged by size:
3862 3211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6899 882
Size: 617 5400 2219
Fragments arranged by size:
5400 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2074 3214 6027 493
Size: 711 870 1140 2813 2702
Fragments arranged by size:
2813 2702 1140 870 711
BsaBI GATnn'nnATC
Cuts at: 3132 3138 3726 8230 3132
Size: 6 588 4504 3138
Fragments arranged by size:
4504 3138 588 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6043 6425 7060 275
Size: 53 83 186 936 4510 382 635 1451
Fragments arranged by size:
4510 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6669 7067 7341 7488 7899 6669
Size: 398 274 147 411 7006
Fragments arranged by size:
7006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 3727 3757 1178
Size: 30 165 30 955 30 1339 30 5657
Fragments arranged by size:
5657 1339 955 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8236
BseYI C'CCAG_C
Cuts at: 2029 3123 7439 7935 2029
Size: 1094 4316 496 2330
Fragments arranged by size:
4316 2330 1094 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3508 1163
Size: 573 1772 5891
Fragments arranged by size:
5891 1772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5686 6447 7475 1540
Size: 275 186 3685 761 1028 2301
Fragments arranged by size:
3685 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2371 6297 6382 7453 2371
Size: 3926 85 1071 3154
Fragments arranged by size:
3926 3154 1071 85
BsmI GAATG_Cn'
Cuts at: 2636 5415 5747 5840 2636
Size: 2779 332 93 5032
Fragments arranged by size:
5032 2779 332 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2371 5171 5555 6297 6382 7453 1391
Size: 399 581 2800 384 742 85 1071 2174
Fragments arranged by size:
2800 2174 1071 742 581 399 384 85
BspEI T'CCGG_A
Cuts at: 7899 7899
Size: 8236
BspHI T'CATG_A
Cuts at: 3142 6125 7855 3142
Size: 2983 1730 3523
Fragments arranged by size:
3523 2983 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5330 5591 844
Size: 4486 261 3489
Fragments arranged by size:
4486 3489 261
BsrBI CCG'CTC
Cuts at: 1394 1823 6123 1394
Size: 429 4300 3507
Fragments arranged by size:
4300 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3514 6731 6905 65
Size: 1183 2266 3217 174 1396
Fragments arranged by size:
3217 2266 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6880 7067 1700
Size: 110 5070 187 2869
Fragments arranged by size:
5070 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3496 4639 96
Size: 3400 1143 3693
Fragments arranged by size:
3693 3400 1143
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8236
BssSI C'ACGA_G
Cuts at: 6296 7308 6296
Size: 1012 7224
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5393 794
Size: 4599 3637
BstBI TT'CG_AA
Cuts at: 2386 2443 7040 2386
Size: 57 4597 3582
Fragments arranged by size:
4597 3582 57
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8236
BstXI CCAn_nnnn'nTGG
Cuts at: 4991 4991
Size: 8236
BstYI r'GATC_y
Cuts at: 1972 2016 3127 4937 5105 5473 5566 5933 6318
Size: 44 1111 1810 168 368 93 367 385
Cuts at: 6318 6335 7787 7873 7885 8231 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1810 1452 1111 385 368 367 346
168 93 86 44 17 12
BstZ17I GTA'TAC
Cuts at: 2785 2931 2785
Size: 146 8090
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7684
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3546 487
Size: 683 56 2320 5177
Fragments arranged by size:
5177 2320 683 56
BtsI GCAGTG_nn'
Cuts at: 4394 5752 6546 6566 4394
Size: 1358 794 20 6064
Fragments arranged by size:
6064 1358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8201
DraI TTT'AAA
Cuts at: 3472 5540 3472
Size: 2068 6168
DraIII CAC_nnn'GTG
Cuts at: 2157 2157
Size: 8236
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7243 809
Size: 507 5927 1802
Fragments arranged by size:
5927 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4165 4981 5156
Size: 54 1425 50 275 18 2335 816 175
Cuts at: 5156 5683 6572 8201 8
Size: 527 889 1629 43
Fragments arranged by size:
2335 1629 1425 889 816 527 275 175
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5683 1537
Size: 275 3871 4090
Fragments arranged by size:
4090 3871 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2191 3186 3343 3470 3972 6166 1353
Size: 838 995 157 127 502 2194 3423
Fragments arranged by size:
3423 2194 995 838 502 157 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6823 7197 7343 387
Size: 1064 138 5234 374 146 1280
Fragments arranged by size:
5234 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2369 2369
Size: 8236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 2922 2964 3324 3672 3747 4894
Size: 393 941 349 42 360 348 75 1147
Cuts at: 4894 6299 6896 7683 7951 1239
Size: 1405 597 787 268 1524
Fragments arranged by size:
1524 1405 1147 941 787 597 393 360
349 348 268 75 42
EcoRI G'AATT_C
Cuts at: 3846 5545 5923 3846
Size: 1699 378 6159
Fragments arranged by size:
6159 1699 378
EcoRV GAT'ATC
Cuts at: 5266 5266
Size: 8236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5296 5296
Size: 8236
FspI TGC'GCA
Cuts at: 6742 6742
Size: 8236
HaeII r_GCGC'y
Cuts at: 1678 2014 7383 1678
Size: 336 5369 2531
Fragments arranged by size:
5369 2531 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2661 2693 2866 2898 4774
Size: 32 135 32 1089 32 173 32 1876
Cuts at: 4774 4806 5074 5106 6877 6909 6951 6983 1373
Size: 32 268 32 1771 32 42 32 2626
Fragments arranged by size:
2626 1876 1771 1089 268 173 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5580 5826 669
Size: 988 281 3642 246 3079
Fragments arranged by size:
3642 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8236
HpaI GTT'AAC
Cuts at: 5826 5826
Size: 8236
KpnI G_GTAC'C
Cuts at: 5561 5561
Size: 8236
MfeI C'AATT_G
Cuts at: 4235 5835 4235
Size: 1600 6636
MluI A'CGCG_T
Cuts at: 8112 8112
Size: 8236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3549 3573 5383 5465 7350 7534 1305
Size: 2244 24 1810 82 1885 184 2007
Fragments arranged by size:
2244 2007 1885 1810 184 82 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4167 4983 5158 10
Size: 54 1425 343 2335 816 175 3088
Fragments arranged by size:
3088 2335 1425 816 343 175 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2052 2426 2792 3855 5027 6194 6553
Size: 648 886 374 366 1063 1172 1167 359
Cuts at: 6553 6712 518
Size: 159 2042
Fragments arranged by size:
2042 1172 1167 1063 886 648 374 366
359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 3973 3992 4142 4370 5652 6329 7477
Size: 423 1944 19 150 228 1282 677 1148
Cuts at: 7477 7722 1606
Size: 245 2120
Fragments arranged by size:
2120 1944 1282 1148 677 423 245 228
150 19
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7684
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8236
NotI GC'GGCC_GC
Cuts at: 5683 5683
Size: 8236
NsiI A_TGCA'T
Cuts at: 3583 3583
Size: 8236
NspI r_CATG'y
Cuts at: 1132 1229 2564 4808 5253 5594 7139 1132
Size: 97 1335 2244 445 341 1545 2229
Fragments arranged by size:
2244 2229 1545 1335 445 341 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8236
PciI A'CATG_T
Cuts at: 2560 5249 7135 2560
Size: 2689 1886 3661
Fragments arranged by size:
3661 2689 1886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2692 1108
Size: 1584 6652
PfoI T'CCnGG_A
Cuts at: 5373 5373
Size: 8236
PmeI GTTT'AAAC
Cuts at: 5540 5540
Size: 8236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3447 3479 6305 6337 7818 7850 3447
Size: 32 2826 32 1481 32 3833
Fragments arranged by size:
3833 2826 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8236
PsiI TTA'TAA
Cuts at: 4202 4656 5806 4202
Size: 454 1150 6632
Fragments arranged by size:
6632 1150 454
PstI C_TGCA'G
Cuts at: 830 2473 2848 4401 5588 830
Size: 1643 375 1553 1187 3478
Fragments arranged by size:
3478 1643 1553 1187 375
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8236
PvuII CAG'CTG
Cuts at: 2029 3992 4142 4370 2029
Size: 1963 150 228 5895
Fragments arranged by size:
5895 1963 228 150
SacI G_AGCT'C
Cuts at: 2371 2371
Size: 8236
SalI G'TCGA_C
Cuts at: 1936 5578 1936
Size: 3642 4594
SapI GCTCTTCn'nnn_
Cuts at: 3186 3186
Size: 8236
SbfI CC_TGCA'GG
Cuts at: 5588 5588
Size: 8236
ScaI AGT'ACT
Cuts at: 3575 5308 6484 8196 3575
Size: 1733 1176 1712 3615
Fragments arranged by size:
3615 1733 1712 1176
SfcI C'TryA_G
Cuts at: 826 1045 2469 2844 4397 5584 6719 7400 7591
Size: 219 1424 375 1553 1187 1135 681 191
Cuts at: 7591 826
Size: 1471
Fragments arranged by size:
1553 1471 1424 1187 1135 681 375 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8236
SmaI CCC'GGG
Cuts at: 1872 5563 1872
Size: 3691 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3165 4870 6340 7241 7503 820
Size: 197 926 1222 1705 1470 901 262 1553
Fragments arranged by size:
1705 1553 1470 1222 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2074 6027 493
Size: 1581 3953 2702
Fragments arranged by size:
3953 2702 1581
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8236
SphI G_CATG'C
Cuts at: 1132 5594 1132
Size: 4462 3774
SspI AAT'ATT
Cuts at: 5 52 4756 6160 5
Size: 47 4704 1404 2081
Fragments arranged by size:
4704 2081 1404 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8236
StyI C'CwwG_G
Cuts at: 513 1065 2963 5675 513
Size: 552 1898 2712 3074
Fragments arranged by size:
3074 2712 1898 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 5301 6263 6280 6433 6618 7063
Size: 1255 840 2881 962 17 153 185 445
Cuts at: 7063 325
Size: 1498
Fragments arranged by size:
2881 1498 1255 962 840 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 3496 3573
Size: 274 80 33 51 1443 171 1348 77
Cuts at: 3573 4639 5306 6482 8194 96
Size: 1066 667 1176 1712 138
Fragments arranged by size:
1712 1443 1348 1176 1066 667 274 171
138 80 77 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3727 3759 5348 5380 8196 8228 3727
Size: 32 1589 32 2816 32 3735
Fragments arranged by size:
3735 2816 1589 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8236
XbaI T'CTAG_A
Cuts at: 5572 5572
Size: 8236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3299 5236 3299
Size: 1937 6299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8236
XmaI C'CCGG_G
Cuts at: 1870 5561 1870
Size: 3691 4545
XmnI GAAnn'nnTTC
Cuts at: 2463 4363 6365 2463
Size: 1900 2002 4334
Fragments arranged by size:
4334 2002 1900
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6044 276
Size: 53 83 186 936 4510 2468
Fragments arranged by size:
4510 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI Bsu36I EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI