Restriction map for
pFN21AB7352
(Circular) (Six-base) MAPSORT of: pFN21AB7352.seq Check: 9052 from: 1 to: 7234
pFN21AB7352 7234 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4369 4369
Size: 7234
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5044 278
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2940 4577 1937
Size: 1003 1637 4594
Fragments arranged by size:
4594 1637 1003
Acc65I G'GTAC_C
Cuts at: 3204 4555 3204
Size: 1351 5883
AclI AA'CG_TT
Cuts at: 1563 5361 5734 1563
Size: 3798 373 3063
Fragments arranged by size:
3798 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2654 3302 3614 3937 4134 5297 6681
Size: 563 459 648 312 323 197 1163 1384
Cuts at: 6681 6949 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1163 648 563 459 323 312
268 197
AfeI AGC'GCT
Cuts at: 2051 2051
Size: 7234
AflII C'TTAA_G
Cuts at: 820 1017 2276 820
Size: 197 1259 5778
Fragments arranged by size:
5778 1259 197
AflIII A'CryG_T
Cuts at: 1203 6133 7110 1203
Size: 4930 977 1327
Fragments arranged by size:
4930 1327 977
AgeI A'CCGG_T
Cuts at: 6065 6065
Size: 7234
AhdI GACnn_n'nnGTC
Cuts at: 5963 5963
Size: 7234
AleI CACnn'nnGTG
Cuts at: 1166 3060 4292 1166
Size: 1894 1232 4108
Fragments arranged by size:
4108 1894 1232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3684 4065 1184
Size: 2500 381 4353
Fragments arranged by size:
4353 2500 381
AlwNI CAG_nnn'CTG
Cuts at: 2173 4018 4312 4977 6549 2173
Size: 1845 294 665 1572 2858
Fragments arranged by size:
2858 1845 1572 665 294
ApaI G_GGCC'C
Cuts at: 3526 4067 3526
Size: 541 6693
ApaLI G'TGCA_C
Cuts at: 5291 6447 5291
Size: 1156 6078
ApoI r'AATT_y
Cuts at: 1493 2285 3191 3748 4543 4769 4921 7030 1493
Size: 792 906 557 795 226 152 2109 1697
Fragments arranged by size:
2109 1697 906 795 792 557 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5788 160
Size: 872 4756 1606
Fragments arranged by size:
4756 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2208 2367 3962 4559 1870
Size: 73 204 61 159 1595 597 4545
Fragments arranged by size:
4545 1595 597 204 159 73 61
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2543 2576 2543
Size: 33 7201
BamHI G'GATC_C
Cuts at: 2079 2854 4564 4931 2079
Size: 775 1710 367 4382
Fragments arranged by size:
4382 1710 775 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3204 4555 6010 618
Size: 325 217 627 1417 1351 1455 1842
Fragments arranged by size:
1842 1455 1417 1351 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2680 2935 3335 3438 3526 4067 4230
Size: 700 589 255 400 103 88 541 163
Cuts at: 4230 4553 1391
Size: 323 4072
Fragments arranged by size:
4072 700 589 541 400 323 255 163
103 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5425 5459 1903
Size: 34 3488 34 3678
Fragments arranged by size:
3678 3488 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3039 3284 5126 6336 3039
Size: 245 1842 1210 3937
Fragments arranged by size:
3937 1842 1210 245
BclI T'GATC_A
Cuts at: 1548 2307 1548
Size: 759 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2413 5845 136
Size: 107 122 71 631 605 741 3432 1525
Fragments arranged by size:
3432 1525 741 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2805 4095 7229 2805
Size: 1290 3134 2810
Fragments arranged by size:
3134 2810 1290
BlpI GC'TnA_GC
Cuts at: 3038 4224 4651 3038
Size: 1186 427 5621
Fragments arranged by size:
5621 1186 427
Bme1580I G_kGCm'C
Cuts at: 1790 3526 4054 4067 4509 5295 6451 1790
Size: 1736 528 13 442 786 1156 2573
Fragments arranged by size:
2573 1736 1156 786 528 442 13
BmgBI CAC'GTC
Cuts at: 1318 3884 1318
Size: 2566 4668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4965 5923
Size: 416 53 83 399 24 2447 1101 958
Cuts at: 5923 442
Size: 1753
Fragments arranged by size:
2447 1753 1101 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 3988 2756
Size: 1232 6002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2183 2870 3571 4505 5894 1239
Size: 815 129 687 701 934 1389 2579
Fragments arranged by size:
2579 1389 934 815 701 687 129
Bpu10I CC'TnA_GC
Cuts at: 2085 2613 4056 4273 4434 2085
Size: 528 1443 217 161 4885
Fragments arranged by size:
4885 1443 528 217 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2952 3985 5359 6224 6522 2952
Size: 1033 1374 865 298 3664
Fragments arranged by size:
3664 1374 1033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5897 882
Size: 617 4398 2219
Fragments arranged by size:
4398 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 4294 5025 493
Size: 711 2237 853 731 2702
Fragments arranged by size:
2702 2237 853 731 711
BsaBI GATnn'nnATC
Cuts at: 7228 7228
Size: 7234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5041 5423 6058 275
Size: 53 83 186 936 3508 382 635 1451
Fragments arranged by size:
3508 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5667 6065 6339 6486 6897 5667
Size: 398 274 147 411 6004
Fragments arranged by size:
6004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2920 2950 1178
Size: 30 165 30 1517 30 5462
Fragments arranged by size:
5462 1517 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2730 3299 1186
Size: 949 595 569 5121
Fragments arranged by size:
5121 949 595 569
BseYI C'CCAG_C
Cuts at: 2223 4414 6437 6933 2223
Size: 2191 2023 496 2524
Fragments arranged by size:
2524 2191 2023 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3323 3644 4055 4397 1163
Size: 573 1587 321 411 342 4000
Fragments arranged by size:
4000 1587 573 411 342 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4684 5445 6473 1540
Size: 275 186 2683 761 1028 2301
Fragments arranged by size:
2683 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 4230 4533 5295 5380 6451 2091
Size: 2139 303 762 85 1071 2874
Fragments arranged by size:
2874 2139 1071 762 303 85
BsmI GAATG_Cn'
Cuts at: 4745 4838 4745
Size: 93 7141
BsmBI CGTCTCn'nnnn_
Cuts at: 2030 4041 2030
Size: 2011 5223
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2680 2935 3335 3438 3526 4054
Size: 399 301 589 255 400 103 88 528
Cuts at: 4054 4067 4230 4509 4533 4553 5295 5380 6451
Size: 13 163 279 24 20 742 85 1071
Cuts at: 6451 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 589 528 400 399 301
279 255 163 103 88 85 24 20
13
BspEI T'CCGG_A
Cuts at: 6897 6897
Size: 7234
BspHI T'CATG_A
Cuts at: 5123 6853 5123
Size: 1730 5504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2781 4369 4589 844
Size: 1937 1588 220 3489
Fragments arranged by size:
3489 1937 1588 220
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 2077 5121 1394
Size: 429 235 19 3044 3507
Fragments arranged by size:
3507 3044 429 235 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 3114 3909 5729 5903 65
Size: 1183 1866 795 1820 174 1396
Fragments arranged by size:
1866 1820 1396 1183 795 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5878 6065 1700
Size: 110 4068 187 2869
Fragments arranged by size:
4068 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7234
BssSI C'ACGA_G
Cuts at: 4397 5294 6306 4397
Size: 897 1012 5325
Fragments arranged by size:
5325 1012 897
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3832 794
Size: 3038 4196
BstBI TT'CG_AA
Cuts at: 6038 6038
Size: 7234
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7234
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2797
Size: 7234
BstYI r'GATC_y
Cuts at: 1972 2079 2805 2854 3594 4095 4359 4564 4931
Size: 107 726 49 740 501 264 205 367
Cuts at: 4931 5316 5333 6785 6871 6883 7229 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 740 726 501 385 367 346
264 205 107 86 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2189 2189
Size: 7234
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5743
Fragments arranged by size:
5743 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2101 2533 487
Size: 683 56 875 432 5188
Fragments arranged by size:
5188 875 683 432 56
BtsI GCAGTG_nn'
Cuts at: 3809 4054 4750 5544 5564 3809
Size: 245 696 794 20 5479
Fragments arranged by size:
5479 794 696 245 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7199
DraI TTT'AAA
Cuts at: 4538 4538
Size: 7234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6241 809
Size: 507 4925 1802
Fragments arranged by size:
4925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2313 3018
Size: 54 1425 50 275 18 276 207 705
Cuts at: 3018 4213 4681 5570 7199 8
Size: 1195 468 889 1629 43
Fragments arranged by size:
1629 1425 1195 889 705 468 276 275
207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4681 1537
Size: 275 2869 4090
Fragments arranged by size:
4090 2869 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3124 3345 3633 4184 4260 4292 5164 1353
Size: 1771 221 288 551 76 32 872 3423
Fragments arranged by size:
3423 1771 872 551 288 221 76 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3266 3608 4367 5821 6195 6341
Size: 1064 138 1677 342 759 1454 374 146
Cuts at: 6341 387
Size: 1280
Fragments arranged by size:
1677 1454 1280 1064 759 374 342 146
138
EcoICRI GAG'CTC
Cuts at: 2089 4228 2089
Size: 2139 5095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2183 2195 2654 2870 3302 3571
Size: 393 422 129 12 459 216 432 269
Cuts at: 3571 3614 3937 4134 4505 5297 5894 6681 6949
Size: 43 323 197 371 792 597 787 268
Cuts at: 6949 1239
Size: 1524
Fragments arranged by size:
1524 792 787 597 459 432 422 393
371 323 269 268 216 197 129 43
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 7234
EcoO109I rG'GnC_Cy
Cuts at: 2213 3487 3522 4063 4423 2213
Size: 1274 35 541 360 5024
Fragments arranged by size:
5024 1274 541 360 35
EcoRI G'AATT_C
Cuts at: 2285 3191 4543 4921 2285
Size: 906 1352 378 4598
Fragments arranged by size:
4598 1352 906 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 7234
FspI TGC'GCA
Cuts at: 5740 5740
Size: 7234
HaeII r_GCGC'y
Cuts at: 1678 2053 3506 6381 1678
Size: 375 1453 2875 2531
Fragments arranged by size:
2875 2531 1453 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2667 2699 2922 2954 3134
Size: 32 135 32 1095 32 223 32 180
Cuts at: 3134 3166 3383 3415 3638 3670 5875 5907 5949
Size: 32 217 32 223 32 2205 32 42
Cuts at: 5949 5981 1373
Size: 32 2626
Fragments arranged by size:
2626 2205 1095 223 223 217 180 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4578 4824 669
Size: 988 281 2640 246 3079
Fragments arranged by size:
3079 2640 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7234
HpaI GTT'AAC
Cuts at: 4824 4824
Size: 7234
KpnI G_GTAC'C
Cuts at: 3208 4559 3208
Size: 1351 5883
MfeI C'AATT_G
Cuts at: 4833 4833
Size: 7234
MluI A'CGCG_T
Cuts at: 7110 7110
Size: 7234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2701 3327 4002 6348 6532 1305
Size: 1396 626 675 2346 184 2007
Fragments arranged by size:
2346 2007 1396 675 626 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 3020 10
Size: 54 1425 343 483 705 4224
Fragments arranged by size:
4224 1425 705 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3060 3699 3722 4292 5192 5551 5710
Size: 648 1894 639 23 570 900 359 159
Cuts at: 5710 518
Size: 2042
Fragments arranged by size:
2042 1894 900 648 639 570 359 159
23
MspA1I CmG'CkG
Cuts at: 1606 2101 2227 2399 2797 4069 4112 4650 5327
Size: 495 126 172 398 1272 43 538 677
Cuts at: 5327 6475 6720 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1272 1148 677 538 495 398 245
172 126 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5743
Fragments arranged by size:
5743 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7234
NotI GC'GGCC_GC
Cuts at: 4681 4681
Size: 7234
NspI r_CATG'y
Cuts at: 1132 1229 3832 4210 4306 4592 6137 1132
Size: 97 2603 378 96 286 1545 2229
Fragments arranged by size:
2603 2229 1545 378 286 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7234
PciI A'CATG_T
Cuts at: 6133 6133
Size: 7234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7234
PfoI T'CCnGG_A
Cuts at: 4365 4365
Size: 7234
PmeI GTTT'AAAC
Cuts at: 4538 4538
Size: 7234
PmlI CAC'GTG
Cuts at: 4294 4294
Size: 7234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 6816 6848 5303
Size: 32 1481 32 5689
Fragments arranged by size:
5689 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3487 4423 3487
Size: 936 6298
PshAI GACnn'nnGTC
Cuts at: 1613 2234 2399 1613
Size: 621 165 6448
Fragments arranged by size:
6448 621 165
PsiI TTA'TAA
Cuts at: 2343 4804 2343
Size: 2461 4773
PspOMI G'GGCC_C
Cuts at: 3522 4063 3522
Size: 541 6693
PstI C_TGCA'G
Cuts at: 830 3084 3453 3576 3837 4044 4144 4586 830
Size: 2254 369 123 261 207 100 442 3478
Fragments arranged by size:
3478 2254 442 369 261 207 123 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7234
PvuII CAG'CTG
Cuts at: 2101 2227 2399 2797 4112 2101
Size: 126 172 398 1315 5223
Fragments arranged by size:
5223 1315 398 172 126
SacI G_AGCT'C
Cuts at: 2091 4230 2091
Size: 2139 5095
SalI G'TCGA_C
Cuts at: 1936 4576 1936
Size: 2640 4594
SapI GCTCTTCn'nnn_
Cuts at: 3633 4292 3633
Size: 659 6575
SbfI CC_TGCA'GG
Cuts at: 4586 4586
Size: 7234
ScaI AGT'ACT
Cuts at: 5482 7194 5482
Size: 1712 5522
SfcI C'TryA_G
Cuts at: 826 1045 2410 3080 3449 3491 3572 3833 4040
Size: 219 1365 670 369 42 81 261 207
Cuts at: 4040 4140 4342 4582 5717 6398 6589 826
Size: 100 202 240 1135 681 191 1471
Fragments arranged by size:
1471 1365 1135 681 670 369 261 240
219 207 202 191 100 81 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7234
SmaI CCC'GGG
Cuts at: 1872 2369 4561 1872
Size: 497 2192 4545
Fragments arranged by size:
4545 2192 497
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2967 4000 5338 6239 6501
Size: 197 926 333 691 1033 1338 901 262
Cuts at: 6501 820
Size: 1553
Fragments arranged by size:
1553 1338 1033 926 901 691 333 262
197
SnaBI TAC'GTA
Cuts at: 493 5025 493
Size: 4532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7234
SphI G_CATG'C
Cuts at: 1132 4306 4592 1132
Size: 3174 286 3774
Fragments arranged by size:
3774 3174 286
SspI AAT'ATT
Cuts at: 5 52 2596 5158 5
Size: 47 2544 2562 2081
Fragments arranged by size:
2562 2544 2081 47
StuI AGG'CCT
Cuts at: 1517 4271 1517
Size: 2754 4480
StyI C'CwwG_G
Cuts at: 513 1065 2004 4476 4673 513
Size: 552 939 2472 197 3074
Fragments arranged by size:
3074 2472 939 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2280 3553 4304 5261 5278 5431 5616
Size: 1255 700 1273 751 957 17 153 185
Cuts at: 5616 6061 325
Size: 445 1498
Fragments arranged by size:
1498 1273 1255 957 751 700 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5480 7192 96
Size: 274 80 33 51 1443 3503 1712 138
Fragments arranged by size:
3503 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 2920 2952 7194 7226 2846
Size: 32 42 32 4242 32 2854
Fragments arranged by size:
4242 2854 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7234
XbaI T'CTAG_A
Cuts at: 4570 4570
Size: 7234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 2308 3325 2128
Size: 180 1017 6037
Fragments arranged by size:
6037 1017 180
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7234
XmaI C'CCGG_G
Cuts at: 1870 2367 4559 1870
Size: 497 2192 4545
Fragments arranged by size:
4545 2192 497
XmnI GAAnn'nnTTC
Cuts at: 5363 5363
Size: 7234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5042 276
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI