Restriction map for
pFN21AB7351
(Circular) (Six-base) MAPSORT of: pFN21AB7351.seq Check: 9767 from: 1 to: 8473
pFN21AB7351 8473 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2590 2590
Size: 8473
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5355 6283 278
Size: 53 83 186 936 3819 928 2468
Fragments arranged by size:
3819 2468 936 928 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 4873 5759 5816 1937
Size: 1110 1826 886 57 4594
Fragments arranged by size:
4594 1826 1110 886 57
Acc65I G'GTAC_C
Cuts at: 4771 5794 4771
Size: 1023 7450
AclI AA'CG_TT
Cuts at: 1563 5680 6600 6973 1563
Size: 4117 920 373 3063
Fragments arranged by size:
4117 3063 920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 2997 3836 3875 4274 4542 4571 4652
Size: 1086 279 839 39 399 268 29 81
Cuts at: 4652 4689 5649 6536 7920 8188 1632
Size: 37 960 887 1384 268 1917
Fragments arranged by size:
1917 1384 1086 960 887 839 399 279
268 268 81 39 37 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8276
AflIII A'CryG_T
Cuts at: 1203 7372 8349 1203
Size: 6169 977 1327
Fragments arranged by size:
6169 1327 977
AgeI A'CCGG_T
Cuts at: 7304 7304
Size: 8473
AhdI GACnn_n'nnGTC
Cuts at: 7202 7202
Size: 8473
AleI CACnn'nnGTG
Cuts at: 1166 4596 1166
Size: 3430 5043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5211 1184
Size: 4027 4446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3994 4026 3994
Size: 32 8441
AlwNI CAG_nnn'CTG
Cuts at: 3356 5755 6216 7788 3356
Size: 2399 461 1572 4041
Fragments arranged by size:
4041 2399 1572 461
ApaLI G'TGCA_C
Cuts at: 6530 7686 6530
Size: 1156 7317
ApoI r'AATT_y
Cuts at: 1493 2132 2443 2698 3135 3841 4168 4318 4431
Size: 639 311 255 437 706 327 150 113
Cuts at: 4431 4911 5087 5537 5782 6008 6160 8269 1493
Size: 480 176 450 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 706 639 480 450 437 327
311 255 245 226 176 152 150 113
AseI AT'TA_AT
Cuts at: 160 1032 5556 7027 160
Size: 872 4524 1471 1606
Fragments arranged by size:
4524 1606 1471 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8473
AvaI C'yCGr_G
Cuts at: 1870 1943 4254 5798 1870
Size: 73 2311 1544 4545
Fragments arranged by size:
4545 2311 1544 73
BamHI G'GATC_C
Cuts at: 5101 5803 6170 5101
Size: 702 367 7404
Fragments arranged by size:
7404 702 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4771 5195 5794 7249
Size: 325 217 627 1622 1362 424 599 1455
Cuts at: 7249 618
Size: 1842
Fragments arranged by size:
1842 1622 1455 1362 627 599 424 325
217
BanII G_rGCy'C
Cuts at: 1391 2092 2622 4244 5646 5792 1391
Size: 701 530 1622 1402 146 4072
Fragments arranged by size:
4072 1622 1402 701 530 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2172 2352 3037 4996 5533 928
Size: 971 273 180 685 1959 537 3868
Fragments arranged by size:
3868 1959 971 685 537 273 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3905 3939 6664 6698 1903
Size: 34 50 34 1884 34 2725 34 3678
Fragments arranged by size:
3678 2725 1884 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6365 7575 6365
Size: 1210 7263
BclI T'GATC_A
Cuts at: 1548 3829 1548
Size: 2281 6192
BfrBI ATG'CAT
Cuts at: 3662 3662
Size: 8473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 7084 136
Size: 107 122 71 631 605 487 4925 1525
Fragments arranged by size:
4925 1525 631 605 487 122 107 71
BglII A'GATC_T
Cuts at: 8468 8468
Size: 8473
BlpI GC'TnA_GC
Cuts at: 3300 5890 3300
Size: 2590 5883
Bme1580I G_kGCm'C
Cuts at: 1790 4429 4759 6534 7690 1790
Size: 2639 330 1775 1156 2573
Fragments arranged by size:
2639 2573 1775 1156 330
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 6204 7162
Size: 416 53 83 399 24 2276 2511 958
Cuts at: 7162 442
Size: 1753
Fragments arranged by size:
2511 2276 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2003 3147 3378 7133 1239
Size: 764 1144 231 3755 2579
Fragments arranged by size:
3755 2579 1144 764 231
Bpu10I CC'TnA_GC
Cuts at: 2265 2517 4219 2265
Size: 252 1702 6519
Fragments arranged by size:
6519 1702 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6598 7463 7761 6598
Size: 865 298 7310
Fragments arranged by size:
7310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5462 7136 882
Size: 617 3963 1674 2219
Fragments arranged by size:
3963 2219 1674 617
BsaAI yAC'GTr
Cuts at: 493 1204 6264 493
Size: 711 5060 2702
Fragments arranged by size:
5060 2702 711
BsaBI GATnn'nnATC
Cuts at: 2252 8467 2252
Size: 6215 2258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4870 5352 6280 6662
Size: 53 83 186 936 3337 482 928 382
Cuts at: 6662 7297 275
Size: 635 1451
Fragments arranged by size:
3337 1451 936 928 635 482 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6906 7304 7578 7725 8136 6906
Size: 398 274 147 411 7243
Fragments arranged by size:
7243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8248
Fragments arranged by size:
8248 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 4029 4493 4496 4820 1186
Size: 1603 1240 464 3 324 4839
Fragments arranged by size:
4839 1603 1240 464 324 3
BseYI C'CCAG_C
Cuts at: 7676 8172 7676
Size: 496 7977
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4818 5367 5748 1163
Size: 573 3082 549 381 3888
Fragments arranged by size:
3888 3082 573 549 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5923 6684 7712 1540
Size: 275 186 3922 761 1028 2301
Fragments arranged by size:
3922 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 3163 3364 3760 4039 4244 6534 6619 7690
Size: 1096 201 396 279 205 2290 85 1071
Cuts at: 7690 2067
Size: 2850
Fragments arranged by size:
2850 2290 1096 1071 396 279 205 201
85
BsmI GAATG_Cn'
Cuts at: 2898 5253 5526 5984 6077 2898
Size: 2355 273 458 93 5294
Fragments arranged by size:
5294 2355 458 273 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3313 3313
Size: 8473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2092 2622 3163 3364 3760 4039
Size: 399 277 25 530 541 201 396 279
Cuts at: 4039 4244 4429 4759 5646 5792 6534 6619 7690
Size: 205 185 330 887 146 742 85 1071
Cuts at: 7690 1391
Size: 2174
Fragments arranged by size:
2174 1071 887 742 541 530 399 396
330 279 277 205 201 185 146 85
25
BspEI T'CCGG_A
Cuts at: 8136 8136
Size: 8473
BspHI T'CATG_A
Cuts at: 4735 5035 5598 6362 8092 4735
Size: 300 563 764 1730 5116
Fragments arranged by size:
5116 1730 764 563 300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2590 4587 4783 5828 844
Size: 1746 1997 196 1045 3489
Fragments arranged by size:
3489 1997 1746 1045 196
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 2094 6360 1394
Size: 429 194 77 4266 3507
Fragments arranged by size:
4266 3507 429 194 77
BsrDI GCAATG_nn'
Cuts at: 65 1248 6968 7142 65
Size: 1183 5720 174 1396
Fragments arranged by size:
5720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7117 7304 1700
Size: 110 5307 187 2869
Fragments arranged by size:
5307 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8473
BssSI C'ACGA_G
Cuts at: 5073 6533 7545 5073
Size: 1460 1012 6001
Fragments arranged by size:
6001 1460 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5146 5248 794
Size: 4352 102 4019
Fragments arranged by size:
4352 4019 102
BstBI TT'CG_AA
Cuts at: 7277 7277
Size: 8473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8473
BstYI r'GATC_y
Cuts at: 1972 2467 2971 5101 5803 6170 6555 6572 8024
Size: 495 504 2130 702 367 385 17 1452
Cuts at: 8024 8110 8122 8468 1972
Size: 86 12 346 1977
Fragments arranged by size:
2130 1977 1452 702 504 495 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3048 3048
Size: 8473
Bsu36I CC'TnA_GG
Cuts at: 2308 5001 2308
Size: 2693 5780
BtgI C'CryG_G
Cuts at: 513 1065 4941 513
Size: 552 3876 4045
Fragments arranged by size:
4045 3876 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7734
Fragments arranged by size:
7734 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 5989 6783 6803 3556
Size: 2433 794 20 5226
Fragments arranged by size:
5226 2433 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8438
DraI TTT'AAA
Cuts at: 5777 5777
Size: 8473
DraIII CAC_nnn'GTG
Cuts at: 2071 5771 2071
Size: 3700 4773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7480 809
Size: 507 6164 1802
Fragments arranged by size:
6164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2160 5920
Size: 54 1425 50 275 18 195 135 3760
Cuts at: 5920 6809 8438 8
Size: 889 1629 43
Fragments arranged by size:
3760 1629 1425 889 275 195 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5920 1537
Size: 275 4108 4090
Fragments arranged by size:
4108 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2044 2136 2496 3420 4443 4497 4671 4797
Size: 691 92 360 924 1023 54 174 126
Cuts at: 4797 4857 5159 6403 1353
Size: 60 302 1244 3423
Fragments arranged by size:
3423 1244 1023 924 691 360 302 174
126 92 60 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5642 7060 7434 7580 387
Size: 1064 138 4053 1418 374 146 1280
Fragments arranged by size:
4053 1418 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4242 4242
Size: 8473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2003 2718 2997 3147 3378 3836 3875
Size: 393 371 715 279 150 231 458 39
Cuts at: 3875 4274 4542 4571 4652 4689 5649 6536 7133
Size: 399 268 29 81 37 960 887 597
Cuts at: 7133 7920 8188 1239
Size: 787 268 1524
Fragments arranged by size:
1524 960 887 787 715 597 458 399
393 371 279 268 268 231 150 81
39 37 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3213 3213
Size: 8473
EcoO109I rG'GnC_Cy
Cuts at: 2708 5752 2708
Size: 3044 5429
EcoRI G'AATT_C
Cuts at: 4431 5782 6160 4431
Size: 1351 378 6744
Fragments arranged by size:
6744 1351 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4719 4719
Size: 8473
FspI TGC'GCA
Cuts at: 3548 6979 3548
Size: 3431 5042
HaeII r_GCGC'y
Cuts at: 1678 3567 7620 1678
Size: 1889 4053 2531
Fragments arranged by size:
4053 2531 1889
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 3583 3615 5444
Size: 32 135 32 923 32 1056 32 1829
Cuts at: 5444 5476 5606 5638 7114 7146 7188 7220 1373
Size: 32 130 32 1476 32 42 32 2626
Fragments arranged by size:
2626 1829 1476 1056 923 135 130 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2291 4711 5206 5584 5760 5817
Size: 988 281 353 2420 495 378 176 57
Cuts at: 5817 6063 669
Size: 246 3079
Fragments arranged by size:
3079 2420 988 495 378 353 281 246
176 57
HindIII A'AGCT_T
Cuts at: 748 2228 748
Size: 1480 6993
HpaI GTT'AAC
Cuts at: 6063 6063
Size: 8473
KpnI G_GTAC'C
Cuts at: 4775 5798 4775
Size: 1023 7450
MfeI C'AATT_G
Cuts at: 6072 6072
Size: 8473
MluI A'CGCG_T
Cuts at: 8349 8349
Size: 8473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 2886 3267 3781 4304 7587 7771 1305
Size: 855 726 381 514 523 3283 184 2007
Fragments arranged by size:
3283 2007 855 726 523 514 381 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6651
Fragments arranged by size:
6651 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 4596 6431 6790 6949 518
Size: 648 1038 2392 1835 359 159 2042
Fragments arranged by size:
2392 2042 1835 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 5698 5889 6566 7714 7959 1606
Size: 424 3668 191 677 1148 245 2120
Fragments arranged by size:
3668 2120 1148 677 424 245 191
NcoI C'CATG_G
Cuts at: 513 1065 4941 513
Size: 552 3876 4045
Fragments arranged by size:
4045 3876 552
NdeI CA'TA_TG
Cuts at: 387 2743 4132 4227 387
Size: 2356 1389 95 4633
Fragments arranged by size:
4633 2356 1389 95
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8473
NotI GC'GGCC_GC
Cuts at: 5920 5920
Size: 8473
NsiI A_TGCA'T
Cuts at: 3664 3664
Size: 8473
NspI r_CATG'y
Cuts at: 1132 1229 5831 7376 1132
Size: 97 4602 1545 2229
Fragments arranged by size:
4602 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4014 1348
Size: 2666 5807
PciI A'CATG_T
Cuts at: 7372 7372
Size: 8473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8473
PfoI T'CCnGG_A
Cuts at: 3680 5722 3680
Size: 2042 6431
PmeI GTTT'AAAC
Cuts at: 5777 5777
Size: 8473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6542 6574 8055 8087 6542
Size: 32 1481 32 6928
Fragments arranged by size:
6928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2708 2708
Size: 8473
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8473
PsiI TTA'TAA
Cuts at: 6043 6043
Size: 8473
PstI C_TGCA'G
Cuts at: 830 3745 5825 830
Size: 2915 2080 3478
Fragments arranged by size:
3478 2915 2080
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8473
PvuII CAG'CTG
Cuts at: 5698 5698
Size: 8473
RsrII CG'GwC_CG
Cuts at: 2044 2044
Size: 8473
SacI G_AGCT'C
Cuts at: 4244 4244
Size: 8473
SalI G'TCGA_C
Cuts at: 1936 5758 5815 1936
Size: 3822 57 4594
Fragments arranged by size:
4594 3822 57
SapI GCTCTTCn'nnn_
Cuts at: 2136 4497 4671 2136
Size: 2361 174 5938
Fragments arranged by size:
5938 2361 174
SbfI CC_TGCA'GG
Cuts at: 5825 5825
Size: 8473
ScaI AGT'ACT
Cuts at: 2614 6721 8433 2614
Size: 4107 1712 2654
Fragments arranged by size:
4107 2654 1712
SfcI C'TryA_G
Cuts at: 826 1045 3202 3741 4653 5631 5821 6956 7637
Size: 219 2157 539 912 978 190 1135 681
Cuts at: 7637 7828 826
Size: 191 1471
Fragments arranged by size:
2157 1471 1135 978 912 681 539 219
191 190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8473
SmaI CCC'GGG
Cuts at: 1872 5800 1872
Size: 3928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6577 7478 7740 820
Size: 197 926 4634 901 262 1553
Fragments arranged by size:
4634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6264 493
Size: 5771 2702
SpeI A'CTAG_T
Cuts at: 152 5264 152
Size: 5112 3361
SphI G_CATG'C
Cuts at: 1132 5831 1132
Size: 4699 3774
SspI AAT'ATT
Cuts at: 5 52 6397 5
Size: 47 6345 2081
Fragments arranged by size:
6345 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8473
StyI C'CwwG_G
Cuts at: 513 1065 3568 4941 5912 513
Size: 552 2503 1373 971 3074
Fragments arranged by size:
3074 2503 1373 971 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 3517 3782 6500 6517 6670 6855
Size: 1255 452 1485 265 2718 17 153 185
Cuts at: 6855 7300 325
Size: 445 1498
Fragments arranged by size:
2718 1498 1485 1255 452 445 265 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2612 6719 8431
Size: 274 80 33 51 1443 635 4107 1712
Cuts at: 8431 96
Size: 138
Fragments arranged by size:
4107 1712 1443 635 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8433 8465 8433
Size: 32 8441
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8473
XbaI T'CTAG_A
Cuts at: 2059 4159 5809 2059
Size: 2100 1650 4723
Fragments arranged by size:
4723 2100 1650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8473
XmaI C'CCGG_G
Cuts at: 1870 5798 1870
Size: 3928 4545
XmnI GAAnn'nnTTC
Cuts at: 2751 3034 3249 3689 5681 6602 2751
Size: 283 215 440 1992 921 4622
Fragments arranged by size:
4622 1992 921 440 283 215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5353 6281 276
Size: 53 83 186 936 3819 928 2468
Fragments arranged by size:
3819 2468 936 928 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstXI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI