Restriction map for
pFN21AB7350
(Circular) (Six-base) MAPSORT of: pFN21AB7350.seq Check: 2820 from: 1 to: 7942
pFN21AB7350 7942 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2702 2915 3302 4070 4688 2702
Size: 213 387 768 618 5956
Fragments arranged by size:
5956 768 618 387 213
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5752 278
Size: 53 83 186 936 4216 2468
Fragments arranged by size:
4216 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5285 1937
Size: 3348 4594
Acc65I G'GTAC_C
Cuts at: 5263 5263
Size: 7942
AclI AA'CG_TT
Cuts at: 1563 6069 6442 1563
Size: 4506 373 3063
Fragments arranged by size:
4506 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2623 3674 3796 4078 4871 5144 6005 7389
Size: 991 1051 122 282 793 273 861 1384
Cuts at: 7389 7657 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1051 991 861 793 282 273
268 122
AfeI AGC'GCT
Cuts at: 2370 3188 2370
Size: 818 7124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7745
AflIII A'CryG_T
Cuts at: 1203 2907 6841 7818 1203
Size: 1704 3934 977 1327
Fragments arranged by size:
3934 1704 1327 977
AgeI A'CCGG_T
Cuts at: 6773 6773
Size: 7942
AhdI GACnn_n'nnGTC
Cuts at: 3700 6671 3700
Size: 2971 4971
AleI CACnn'nnGTG
Cuts at: 1166 4790 1166
Size: 3624 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4278 4492 1184
Size: 3094 214 4634
Fragments arranged by size:
4634 3094 214
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4400 4432 4400
Size: 32 7910
AlwNI CAG_nnn'CTG
Cuts at: 2338 2577 2671 3232 3435 3526 4594 5685 7257
Size: 239 94 561 203 91 1068 1091 1572
Cuts at: 7257 2338
Size: 3023
Fragments arranged by size:
3023 1572 1091 1068 561 239 203 94
91
ApaI G_GGCC'C
Cuts at: 2260 4512 2260
Size: 2252 5690
ApaLI G'TGCA_C
Cuts at: 5999 7155 5999
Size: 1156 6786
ApoI r'AATT_y
Cuts at: 1493 2530 5251 5477 5629 7738 1493
Size: 1037 2721 226 152 2109 1697
Fragments arranged by size:
2721 2109 1697 1037 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6496 160
Size: 872 5464 1606
Fragments arranged by size:
5464 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7942
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 2711 5267 1870
Size: 73 711 57 2556 4545
Fragments arranged by size:
4545 2556 711 73 57
BamHI G'GATC_C
Cuts at: 2715 5272 5639 2715
Size: 2557 367 5018
Fragments arranged by size:
5018 2557 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2814 2989 3514 4203 4487
Size: 325 217 627 1027 175 525 689 284
Cuts at: 4487 5263 6718 618
Size: 776 1455 1842
Fragments arranged by size:
1842 1455 1027 776 689 627 525 325
284 217 175
BanII G_rGCy'C
Cuts at: 1391 2260 2828 3119 3652 4512 4716 5261 1391
Size: 869 568 291 533 860 204 545 4072
Fragments arranged by size:
4072 869 860 568 545 533 291 204
BbeI G_GCGC'C
Cuts at: 4491 4491
Size: 7942
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2629 4144 5038 928
Size: 971 730 1515 894 3832
Fragments arranged by size:
3832 1515 971 894 730
BbvCI CC'TCA_GC
Cuts at: 2455 2894 3507 5168 2455
Size: 439 613 1661 5229
Fragments arranged by size:
5229 1661 613 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3736 3770 6133 6167 1903
Size: 34 1799 34 2363 34 3678
Fragments arranged by size:
3678 2363 1799 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5834 7044 5834
Size: 1210 6732
BclI T'GATC_A
Cuts at: 1548 4917 1548
Size: 3369 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2553 6553 136
Size: 107 122 71 631 605 881 4000 1525
Fragments arranged by size:
4000 1525 881 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4413 7937 4413
Size: 3524 4418
BlpI GC'TnA_GC
Cuts at: 2759 3068 3587 5359 2759
Size: 309 519 1772 5342
Fragments arranged by size:
5342 1772 519 309
Bme1580I G_kGCm'C
Cuts at: 1790 2260 2994 3791 4512 4685 5231 6003 7159
Size: 470 734 797 721 173 546 772 1156
Cuts at: 7159 1790
Size: 2573
Fragments arranged by size:
2573 1156 797 772 734 721 546 470
173
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2109 2823 2929
Size: 416 53 83 399 24 692 714 106
Cuts at: 2929 4697 5673 6631 442
Size: 1768 976 958 1753
Fragments arranged by size:
1768 1753 976 958 714 692 416 399
106 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7942
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2784 3175 3325 5011 2784
Size: 391 150 1686 5715
Fragments arranged by size:
5715 1686 391 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2413 3133 3141 4488 5021 6602 1239
Size: 1092 82 720 8 1347 533 1581 2579
Fragments arranged by size:
2579 1581 1347 1092 720 533 82 8
Bpu10I CC'TnA_GC
Cuts at: 2455 2798 2894 3507 5168 2455
Size: 343 96 613 1661 5229
Fragments arranged by size:
5229 1661 613 343 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4311 4710 6067 6932 7230 4311
Size: 399 1357 865 298 5023
Fragments arranged by size:
5023 1357 865 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2101 2380 3708 3761 4855 6605 882
Size: 617 602 279 1328 53 1094 1750 2219
Fragments arranged by size:
2219 1750 1328 1094 617 602 279 53
BsaAI yAC'GTr
Cuts at: 493 1204 3210 3346 3604 5733 493
Size: 711 2006 136 258 2129 2702
Fragments arranged by size:
2702 2129 2006 711 258 136
BsaBI GATnn'nnATC
Cuts at: 7936 7936
Size: 7942
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3306 4488 5043 5749
Size: 53 83 186 936 1773 1182 555 706
Cuts at: 5749 6131 6766 275
Size: 382 635 1451
Fragments arranged by size:
1773 1451 1182 936 706 635 555 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4297 6375 6773 7047 7194 7605 4297
Size: 2078 398 274 147 411 4634
Fragments arranged by size:
4634 2078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2016 2046 2427 2457 2714
Size: 30 165 30 613 30 381 30 257
Cuts at: 2714 2744 2815 2845 3382 3412 3863 3893 4400
Size: 30 71 30 537 30 451 30 507
Cuts at: 4400 4430 1178
Size: 30 4690
Fragments arranged by size:
4690 613 537 507 451 381 257 165
71 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2303 3403 3524 5203 1186
Size: 1114 3 1100 121 1679 3925
Fragments arranged by size:
3925 1679 1114 1100 121 3
BseYI C'CCAG_C
Cuts at: 2056 2733 3083 4024 4885 4909 4966 5126 7145
Size: 677 350 941 861 24 57 160 2019
Cuts at: 7145 7641 2056
Size: 496 2357
Fragments arranged by size:
2357 2019 941 861 677 496 350 160
57 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3421 1163
Size: 573 1685 5684
Fragments arranged by size:
5684 1685 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5392 6153 7181 1540
Size: 275 186 3391 761 1028 2301
Fragments arranged by size:
3391 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 3593 4668 6003 6088 7159 3318
Size: 275 1075 1335 85 1071 4101
Fragments arranged by size:
4101 1335 1075 1071 275 85
BsiWI C'GTAC_G
Cuts at: 3210 3210
Size: 7942
BsmI GAATG_Cn'
Cuts at: 4757 5199 5453 5546 4757
Size: 442 254 93 7153
Fragments arranged by size:
7153 442 254 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2905 2905
Size: 7942
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2260 2828 2994 3119 3318 3593 3652
Size: 399 470 568 166 125 199 275 59
Cuts at: 3652 3791 4512 4668 4685 4716 5231 5261 6003
Size: 139 721 156 17 31 515 30 742
Cuts at: 6003 6088 7159 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 721 568 515 470 399
275 199 166 156 139 125 85 59
31 30 17
BspEI T'CCGG_A
Cuts at: 4297 7605 4297
Size: 3308 4634
BspHI T'CATG_A
Cuts at: 5831 7561 5831
Size: 1730 6212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 2915 3231 3302 4070 4688 5297 844
Size: 1858 213 316 71 768 618 609 3489
Fragments arranged by size:
3489 1858 768 618 609 316 213 71
BsrBI CCG'CTC
Cuts at: 1394 1823 4310 4522 5829 1394
Size: 429 2487 212 1307 3507
Fragments arranged by size:
3507 2487 1307 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 6437 6611 65
Size: 1183 5189 174 1396
Fragments arranged by size:
5189 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3421 6586 6773 1700
Size: 110 1611 3165 187 2869
Fragments arranged by size:
3165 2869 1611 187 110
BsrGI T'GTAC_A
Cuts at: 96 2386 96
Size: 2290 5652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7942
BssSI C'ACGA_G
Cuts at: 6002 7014 6002
Size: 1012 6930
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3786 794
Size: 2992 4950
BstBI TT'CG_AA
Cuts at: 6746 6746
Size: 7942
BstEII G'GTnAC_C
Cuts at: 1184 2061 4082 1184
Size: 877 2021 5044
Fragments arranged by size:
5044 2021 877
BstXI CCAn_nnnn'nTGG
Cuts at: 2351 2624 3616 3848 5143 2351
Size: 273 992 232 1295 5150
Fragments arranged by size:
5150 1295 992 273 232
BstYI r'GATC_y
Cuts at: 1972 2324 2715 3522 4413 5163 5272 5639 6024
Size: 352 391 807 891 750 109 367 385
Cuts at: 6024 6041 7493 7579 7591 7937 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 891 807 750 391 385 367
352 346 109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2810 3300 4739 2810
Size: 490 1439 6013
Fragments arranged by size:
6013 1439 490
BtgI C'CryG_G
Cuts at: 513 1065 2004 2878 3105 4839 4998 5204 513
Size: 552 939 874 227 1734 159 206 3251
Fragments arranged by size:
3251 1734 939 874 552 227 206 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7203
Fragments arranged by size:
7203 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 3899 4233 4260 5458 6252 6272 2024
Size: 1875 334 27 1198 794 20 3694
Fragments arranged by size:
3694 1875 1198 794 334 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2603 2638 4674 4709 529
Size: 35 2039 35 2036 35 3762
Fragments arranged by size:
3762 2039 2036 35 35 35
DraI TTT'AAA
Cuts at: 5246 5246
Size: 7942
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2860 6949 809
Size: 507 1544 4089 1802
Fragments arranged by size:
4089 1802 1544 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2227 2881
Size: 54 1425 50 275 18 207 190 654
Cuts at: 2881 3340 3606 4218 4464 4563 4806 4842 4974
Size: 459 266 612 246 99 243 36 132
Cuts at: 4974 5389 6278 7907 8
Size: 415 889 1629 43
Fragments arranged by size:
1629 1425 889 654 612 459 415 275
266 246 243 207 190 132 99 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 5389 1537
Size: 275 3577 4090
Fragments arranged by size:
4090 3577 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2470 2484 4175 5872 1353
Size: 699 418 14 1691 1697 3423
Fragments arranged by size:
3423 1697 1691 699 418 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4415 6529 6903 7049 387
Size: 1064 138 2826 2114 374 146 1280
Fragments arranged by size:
2826 2114 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2413 2623 3133 3141 3674 3796
Size: 393 699 82 210 510 8 533 122
Cuts at: 3796 4078 4488 4871 5021 5144 6005 6602 7389
Size: 282 410 383 150 123 861 597 787
Cuts at: 7389 7657 1239
Size: 268 1524
Fragments arranged by size:
1524 861 787 699 597 533 510 410
393 383 282 268 210 150 123 122
82 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 2975 4742 2282
Size: 693 1767 5482
Fragments arranged by size:
5482 1767 693
EcoO109I rG'GnC_Cy
Cuts at: 2141 2682 3253 3355 3682 4508 4509 4591 4880
Size: 541 571 102 327 826 1 82 289
Cuts at: 4880 2141
Size: 5203
Fragments arranged by size:
5203 826 571 541 327 289 102 82
1
EcoRI G'AATT_C
Cuts at: 5251 5629 5251
Size: 378 7564
FspI TGC'GCA
Cuts at: 5099 6448 5099
Size: 1349 6593
HaeII r_GCGC'y
Cuts at: 1678 2372 3190 4319 4358 4491 4900 7089 1678
Size: 694 818 1129 39 133 409 2189 2531
Fragments arranged by size:
2531 2189 1129 818 694 409 133 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2425 2457 2758 2790 2815
Size: 32 135 32 853 32 301 32 25
Cuts at: 2815 2847 3686 3718 4095 4127 6583 6615 6657
Size: 32 839 32 377 32 2456 32 42
Cuts at: 6657 6689 1373
Size: 32 2626
Fragments arranged by size:
2626 2456 853 839 377 301 135 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2518 5286 5532 669
Size: 988 281 580 2768 246 3079
Fragments arranged by size:
3079 2768 988 580 281 246
HindIII A'AGCT_T
Cuts at: 748 3135 5091 748
Size: 2387 1956 3599
Fragments arranged by size:
3599 2387 1956
HpaI GTT'AAC
Cuts at: 5532 5532
Size: 7942
KasI G'GCGC_C
Cuts at: 4487 4487
Size: 7942
KpnI G_GTAC'C
Cuts at: 5267 5267
Size: 7942
MfeI C'AATT_G
Cuts at: 5541 5541
Size: 7942
MluI A'CGCG_T
Cuts at: 7818 7818
Size: 7942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2928 3594 4740 7056 7240 1305
Size: 1623 666 1146 2316 184 2007
Fragments arranged by size:
2316 2007 1623 1146 666 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 3342 3608 4220 4565
Size: 54 1425 343 207 1303 266 612 345
Cuts at: 4565 4808 4844 4976 10
Size: 243 36 132 2976
Fragments arranged by size:
2976 1425 1303 612 345 343 266 243
207 132 54 36
MslI CAynn'nnrTG
Cuts at: 518 1166 4790 4817 4844 5900 6259 6418 518
Size: 648 3624 27 27 1056 359 159 2042
Fragments arranged by size:
3624 2042 1056 648 359 159 27 27
MspA1I CmG'CkG
Cuts at: 1606 2737 3031 3067 3107 3435 3535 3844 4612
Size: 1131 294 36 40 328 100 309 768
Cuts at: 4612 4633 5172 5358 6035 7183 7428 1606
Size: 21 539 186 677 1148 245 2120
Fragments arranged by size:
2120 1148 1131 768 677 539 328 309
294 245 186 100 40 36 21
NarI GG'CG_CC
Cuts at: 4488 4488
Size: 7942
NcoI C'CATG_G
Cuts at: 513 1065 2004 4839 5204 513
Size: 552 939 2835 365 3251
Fragments arranged by size:
3251 2835 939 552 365
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7942
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7942
NotI GC'GGCC_GC
Cuts at: 5389 5389
Size: 7942
NspI r_CATG'y
Cuts at: 1132 1229 2911 3780 5191 5300 6845 1132
Size: 97 1682 869 1411 109 1545 2229
Fragments arranged by size:
2229 1682 1545 1411 869 109 97
PasI CC'CwG_GG
Cuts at: 1348 2112 2819 2953 3735 4708 1348
Size: 764 707 134 782 973 4582
Fragments arranged by size:
4582 973 782 764 707 134
PciI A'CATG_T
Cuts at: 2907 6841 2907
Size: 3934 4008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7942
PfoI T'CCnGG_A
Cuts at: 2337 2429 3276 2337
Size: 92 847 7003
Fragments arranged by size:
7003 847 92
PmeI GTTT'AAAC
Cuts at: 5246 5246
Size: 7942
PmlI CAC'GTG
Cuts at: 3346 3346
Size: 7942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 6011 6043 7524 7556 4400
Size: 32 1579 32 1481 32 4786
Fragments arranged by size:
4786 1579 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2682 3253 3682 4880 2141
Size: 541 571 429 1198 5203
Fragments arranged by size:
5203 1198 571 541 429
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7942
PsiI TTA'TAA
Cuts at: 5512 5512
Size: 7942
PspOMI G'GGCC_C
Cuts at: 2256 4508 2256
Size: 2252 5690
PstI C_TGCA'G
Cuts at: 830 2127 2707 2742 2847 3228 3486 3837 3906
Size: 1297 580 35 105 381 258 351 69
Cuts at: 3906 5294 830
Size: 1388 3478
Fragments arranged by size:
3478 1388 1297 580 381 351 258 105
69 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7942
PvuII CAG'CTG
Cuts at: 2737 3031 3067 3435 3535 3844 4612 4633 5172
Size: 294 36 368 100 309 768 21 539
Cuts at: 5172 2737
Size: 5507
Fragments arranged by size:
5507 768 539 368 309 294 100 36
21
SacII CC_GC'GG
Cuts at: 3108 3108
Size: 7942
SalI G'TCGA_C
Cuts at: 1936 5284 1936
Size: 3348 4594
SanDI GG'GwC_CC
Cuts at: 2141 2682 3253 4880 2141
Size: 541 571 1627 5203
Fragments arranged by size:
5203 1627 571 541
SbfI CC_TGCA'GG
Cuts at: 5294 5294
Size: 7942
ScaI AGT'ACT
Cuts at: 6190 7902 6190
Size: 1712 6230
SexAI A'CCwGG_T
Cuts at: 3698 3698
Size: 7942
SfcI C'TryA_G
Cuts at: 826 1045 2123 2703 2738 2843 3224 3482 3833
Size: 219 1078 580 35 105 381 258 351
Cuts at: 3833 3871 3902 5290 6425 7106 7297 826
Size: 38 31 1388 1135 681 191 1471
Fragments arranged by size:
1471 1388 1135 1078 681 580 381 351
258 219 191 105 38 35 31
SfoI GGC'GCC
Cuts at: 4489 4489
Size: 7942
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7942
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7942
SmaI CCC'GGG
Cuts at: 1872 2656 2713 5269 1872
Size: 784 57 2556 4545
Fragments arranged by size:
4545 2556 784 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4326 4725 6046 6947 7209 820
Size: 197 926 2383 399 1321 901 262 1553
Fragments arranged by size:
2383 1553 1321 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 5733 493
Size: 5240 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7942
SphI G_CATG'C
Cuts at: 1132 3780 5300 1132
Size: 2648 1520 3774
Fragments arranged by size:
3774 2648 1520
SspI AAT'ATT
Cuts at: 5 52 5866 5
Size: 47 5814 2081
Fragments arranged by size:
5814 2081 47
StuI AGG'CCT
Cuts at: 1517 2476 1517
Size: 959 6983
StyI C'CwwG_G
Cuts at: 513 1065 2004 2238 2471 3333 3609 4839 5204
Size: 552 939 234 233 862 276 1230 365
Cuts at: 5204 5381 513
Size: 177 3074
Fragments arranged by size:
3074 1230 939 862 552 365 276 234
233 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2832 4361 4957 5969 5986 6139 6324
Size: 1255 1252 1529 596 1012 17 153 185
Cuts at: 6324 6769 325
Size: 445 1498
Fragments arranged by size:
1529 1498 1255 1252 1012 596 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 2931 4377
Size: 274 80 33 51 1443 409 545 1446
Cuts at: 4377 6188 7900 96
Size: 1811 1712 138
Fragments arranged by size:
1811 1712 1446 1443 545 409 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2032 2048 2064 2427 2459 3237 3269 3382
Size: 16 16 16 363 32 778 32 113
Cuts at: 3382 3414 7902 7934 2016
Size: 32 4488 32 2024
Fragments arranged by size:
4488 2024 778 363 113 32 32 32
32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 2301 3410 3680 1316
Size: 985 1109 270 5578
Fragments arranged by size:
5578 1109 985 270
XbaI T'CTAG_A
Cuts at: 5278 5278
Size: 7942
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2409 2621 3091 3601 3925 4051 5140 2409
Size: 212 470 510 324 126 1089 5211
Fragments arranged by size:
5211 1089 510 470 324 212 126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7942
XmaI C'CCGG_G
Cuts at: 1870 2654 2711 5267 1870
Size: 784 57 2556 4545
Fragments arranged by size:
4545 2556 784 57
XmnI GAAnn'nnTTC
Cuts at: 5199 6071 5199
Size: 872 7070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5750 276
Size: 53 83 186 936 4216 2468
Fragments arranged by size:
4216 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI