(Circular) (Six-base) MAPSORT of: pFN21AB7348.seq Check: 854 from: 1 to: 8062 pFN21AB7348 8062 bp With 182 enzymes: SgfI * May 6, 2010 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4930 4930 Size: 8062 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5872 278 Size: 53 83 186 936 4336 2468 Fragments arranged by size: 4336 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2686 3108 3268 4622 5405 1937 Size: 749 422 160 1354 783 4594 Fragments arranged by size: 4594 1354 783 749 422 160 Acc65I G'GTAC_C Cuts at: 5383 5383 Size: 8062 AclI AA'CG_TT Cuts at: 1563 6189 6562 1563 Size: 4626 373 3063 Fragments arranged by size: 4626 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3045 4681 4714 5177 6125 7509 7777 1632 Size: 1413 1636 33 463 948 1384 268 1917 Fragments arranged by size: 1917 1636 1413 1384 948 463 268 33 AflII C'TTAA_G Cuts at: 820 1017 2111 3830 820 Size: 197 1094 1719 5052 Fragments arranged by size: 5052 1719 1094 197 AflIII A'CryG_T Cuts at: 1203 6961 7938 1203 Size: 5758 977 1327 Fragments arranged by size: 5758 1327 977 AgeI A'CCGG_T Cuts at: 6893 6893 Size: 8062 AhdI GACnn_n'nnGTC Cuts at: 6791 6791 Size: 8062 AleI CACnn'nnGTG Cuts at: 1166 2507 5015 1166 Size: 1341 2508 4213 Fragments arranged by size: 4213 2508 1341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2229 1184 Size: 1045 7017 AlwNI CAG_nnn'CTG Cuts at: 5805 7377 5805 Size: 1572 6490 ApaI G_GGCC'C Cuts at: 3281 3281 Size: 8062 ApaLI G'TGCA_C Cuts at: 2521 2790 2821 3622 6119 7275 2521 Size: 269 31 801 2497 1156 3308 Fragments arranged by size: 3308 2497 1156 801 269 31 ApoI r'AATT_y Cuts at: 1493 3079 3188 4007 4168 4300 4377 4537 4575 Size: 1586 109 819 161 132 77 160 38 Cuts at: 4575 4681 4864 5371 5597 5749 7858 1493 Size: 106 183 507 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 819 507 226 183 161 160 152 132 109 106 77 38 AseI AT'TA_AT Cuts at: 160 1032 3009 6616 160 Size: 872 1977 3607 1606 Fragments arranged by size: 3607 1977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8062 AvaI C'yCGr_G Cuts at: 1870 1943 5387 1870 Size: 73 3444 4545 Fragments arranged by size: 4545 3444 73 BamHI G'GATC_C Cuts at: 4690 5392 5759 4690 Size: 702 367 6993 Fragments arranged by size: 6993 702 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 3098 5383 6838 618 Size: 325 217 627 244 1067 2285 1455 1842 Fragments arranged by size: 2285 1842 1455 1067 627 325 244 217 BanII G_rGCy'C Cuts at: 1391 2055 3281 4159 5196 5381 1391 Size: 664 1226 878 1037 185 4072 Fragments arranged by size: 4072 1226 1037 878 664 185 BbeI G_GCGC'C Cuts at: 2035 2035 Size: 8062 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3237 3648 3951 928 Size: 971 1338 411 303 5039 Fragments arranged by size: 5039 1338 971 411 303 BbvCI CC'TCA_GC Cuts at: 4899 4899 Size: 8062 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6253 6287 1903 Size: 34 4316 34 3678 Fragments arranged by size: 4316 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5031 5954 7164 5031 Size: 923 1210 5929 Fragments arranged by size: 5929 1210 923 BclI T'GATC_A Cuts at: 1548 2511 2766 2962 4273 5205 1548 Size: 963 255 196 1311 932 4405 Fragments arranged by size: 4405 1311 963 932 255 196 BfrBI ATG'CAT Cuts at: 2643 3263 3903 4916 2643 Size: 620 640 1013 5789 Fragments arranged by size: 5789 1013 640 620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6673 136 Size: 107 122 71 631 605 5001 1525 Fragments arranged by size: 5001 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5036 8057 5036 Size: 3021 5041 BlpI GC'TnA_GC Cuts at: 2571 5479 2571 Size: 2908 5154 Bme1580I G_kGCm'C Cuts at: 1790 2525 2794 2825 3103 3281 3626 6123 7279 Size: 735 269 31 278 178 345 2497 1156 Cuts at: 7279 1790 Size: 2573 Fragments arranged by size: 2573 2497 1156 735 345 278 269 178 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2798 5091 5793 Size: 416 53 83 399 24 1381 2293 702 Cuts at: 5793 6751 442 Size: 958 1753 Fragments arranged by size: 2293 1753 1381 958 702 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2978 1056 Size: 1922 6140 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5245 5245 Size: 8062 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 3837 6722 1239 Size: 819 1779 2885 2579 Fragments arranged by size: 2885 2579 1779 819 Bpu10I CC'TnA_GC Cuts at: 4899 4899 Size: 8062 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 5375 6187 7052 7350 2477 Size: 2898 812 865 298 3189 Fragments arranged by size: 3189 2898 865 812 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2927 4196 6725 882 Size: 617 1428 1269 2529 2219 Fragments arranged by size: 2529 2219 1428 1269 617 BsaAI yAC'GTr Cuts at: 493 1204 5853 493 Size: 711 4649 2702 Fragments arranged by size: 4649 2702 711 BsaBI GATnn'nnATC Cuts at: 5222 8056 5222 Size: 2834 5228 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2032 5869 6251 6886 Size: 53 83 186 936 499 3837 382 635 Cuts at: 6886 275 Size: 1451 Fragments arranged by size: 3837 1451 936 635 499 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6495 6893 7167 7314 7725 6495 Size: 398 274 147 411 6832 Fragments arranged by size: 6832 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7837 Fragments arranged by size: 7837 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 2394 3240 3447 1186 Size: 1193 15 846 207 5801 Fragments arranged by size: 5801 1193 846 207 15 BseYI C'CCAG_C Cuts at: 7265 7761 7265 Size: 496 7566 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 3639 1163 Size: 573 705 1198 5586 Fragments arranged by size: 5586 1198 705 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5512 6273 7301 1540 Size: 275 186 3511 761 1028 2301 Fragments arranged by size: 3511 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2525 2794 2825 3626 4159 5196 6123 6208 7279 Size: 269 31 801 533 1037 927 85 1071 Cuts at: 7279 2525 Size: 3308 Fragments arranged by size: 3308 1071 1037 927 801 533 269 85 31 BsmI GAATG_Cn' Cuts at: 2645 3905 3934 5573 5666 2645 Size: 1260 29 1639 93 5041 Fragments arranged by size: 5041 1639 1260 93 29 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2525 2794 2825 3103 3281 3626 Size: 399 265 470 269 31 278 178 345 Cuts at: 3626 4159 5196 5381 6123 6208 7279 1391 Size: 533 1037 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 1037 742 533 470 399 345 278 269 265 185 178 85 31 BspEI T'CCGG_A Cuts at: 7725 7725 Size: 8062 BspHI T'CATG_A Cuts at: 2929 4128 4860 5951 7681 2929 Size: 1199 732 1091 1730 3310 Fragments arranged by size: 3310 1730 1199 1091 732 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4930 5417 844 Size: 4086 487 3489 Fragments arranged by size: 4086 3489 487 BsrBI CCG'CTC Cuts at: 1394 1823 5949 1394 Size: 429 4126 3507 Fragments arranged by size: 4126 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2514 2543 4074 4423 4508 4706 4893 Size: 1183 1266 29 1531 349 85 198 187 Cuts at: 4893 6557 6731 65 Size: 1664 174 1396 Fragments arranged by size: 1664 1531 1396 1266 1183 349 198 187 174 85 29 BsrFI r'CCGG_y Cuts at: 1700 1810 5151 6706 6893 1700 Size: 110 3341 1555 187 2869 Fragments arranged by size: 3341 2869 1555 187 110 BsrGI T'GTAC_A Cuts at: 96 3151 96 Size: 3055 5007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8062 BssSI C'ACGA_G Cuts at: 6122 7134 6122 Size: 1012 7050 BstAPI GCAn_nnn'nTGC Cuts at: 794 2554 794 Size: 1760 6302 BstBI TT'CG_AA Cuts at: 6866 6866 Size: 8062 BstEII G'GTnAC_C Cuts at: 1184 3437 1184 Size: 2253 5809 BstYI r'GATC_y Cuts at: 1972 4175 4690 5036 5161 5392 5759 6144 6161 Size: 2203 515 346 125 231 367 385 17 Cuts at: 6161 7613 7699 7711 8057 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2203 1977 1452 515 385 367 346 346 231 125 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2018 2018 Size: 8062 BtgI C'CryG_G Cuts at: 513 1065 3570 513 Size: 552 2505 5005 Fragments arranged by size: 5005 2505 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7323 Fragments arranged by size: 7323 683 56 BtsI GCAGTG_nn' Cuts at: 3651 4502 5578 6372 6392 3651 Size: 851 1076 794 20 5321 Fragments arranged by size: 5321 1076 851 794 20 ClaI AT'CG_AT Cuts at: 1554 3018 1554 Size: 1464 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2845 2880 529 Size: 35 2281 35 5711 Fragments arranged by size: 5711 2281 35 35 DraI TTT'AAA Cuts at: 5366 5366 Size: 8062 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7069 809 Size: 507 5753 1802 Fragments arranged by size: 5753 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5509 6398 8027 Size: 54 1425 50 275 18 3679 889 1629 Cuts at: 8027 8 Size: 43 Fragments arranged by size: 3679 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5509 1537 Size: 275 3697 4090 Fragments arranged by size: 4090 3697 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2664 4919 5992 1353 Size: 1311 2255 1073 3423 Fragments arranged by size: 3423 2255 1311 1073 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6649 7023 7169 387 Size: 1064 138 5060 374 146 1280 Fragments arranged by size: 5060 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4157 5194 4157 Size: 1037 7025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 3045 3837 4681 4714 5177 6125 Size: 393 426 987 792 844 33 463 948 Cuts at: 6125 6722 7509 7777 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 987 948 844 792 787 597 463 426 393 268 33 EcoNI CCTnn'n_nnAGG Cuts at: 3391 3391 Size: 8062 EcoO109I rG'GnC_Cy Cuts at: 3277 3278 3277 Size: 1 8061 EcoRI G'AATT_C Cuts at: 4007 4300 5371 5749 4007 Size: 293 1071 378 6320 Fragments arranged by size: 6320 1071 378 293 EcoRV GAT'ATC Cuts at: 2880 4750 2880 Size: 1870 6192 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3458 3458 Size: 8062 FspI TGC'GCA Cuts at: 6568 6568 Size: 8062 HaeII r_GCGC'y Cuts at: 1678 2035 7209 1678 Size: 357 5174 2531 Fragments arranged by size: 5174 2531 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2389 2421 3329 3361 3826 Size: 32 135 32 817 32 908 32 465 Cuts at: 3826 3858 4991 5023 6703 6735 6777 6809 1373 Size: 32 1133 32 1680 32 42 32 2626 Fragments arranged by size: 2626 1680 1133 908 817 465 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2911 5406 5652 669 Size: 988 281 973 2495 246 3079 Fragments arranged by size: 3079 2495 988 973 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8062 HpaI GTT'AAC Cuts at: 2911 5652 2911 Size: 2741 5321 KasI G'GCGC_C Cuts at: 2031 2031 Size: 8062 KpnI G_GTAC'C Cuts at: 5387 5387 Size: 8062 MfeI C'AATT_G Cuts at: 4512 4851 5661 4512 Size: 339 810 6913 Fragments arranged by size: 6913 810 339 MluI A'CGCG_T Cuts at: 7938 7938 Size: 8062 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4408 4572 7176 7360 1305 Size: 3103 164 2604 184 2007 Fragments arranged by size: 3103 2604 2007 184 164 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6240 Fragments arranged by size: 6240 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2507 4199 4457 5015 6020 6379 Size: 648 1251 90 1692 258 558 1005 359 Cuts at: 6379 6538 518 Size: 159 2042 Fragments arranged by size: 2042 1692 1251 1005 648 558 359 258 159 90 MspA1I CmG'CkG Cuts at: 1606 2507 5478 6155 7303 7548 1606 Size: 901 2971 677 1148 245 2120 Fragments arranged by size: 2971 2120 1148 901 677 245 NarI GG'CG_CC Cuts at: 2032 2032 Size: 8062 NcoI C'CATG_G Cuts at: 513 1065 3570 513 Size: 552 2505 5005 Fragments arranged by size: 5005 2505 552 NdeI CA'TA_TG Cuts at: 387 2137 4161 387 Size: 1750 2024 4288 Fragments arranged by size: 4288 2024 1750 NheI G'CTAG_C Cuts at: 1052 2974 1052 Size: 1922 6140 NotI GC'GGCC_GC Cuts at: 5509 5509 Size: 8062 NsiI A_TGCA'T Cuts at: 2645 3265 3905 4918 2645 Size: 620 640 1013 5789 Fragments arranged by size: 5789 1013 640 620 NspI r_CATG'y Cuts at: 1132 1229 3267 3931 4916 5420 6965 1132 Size: 97 2038 664 985 504 1545 2229 Fragments arranged by size: 2229 2038 1545 985 664 504 97 PasI CC'CwG_GG Cuts at: 1348 2056 2848 1348 Size: 708 792 6562 Fragments arranged by size: 6562 792 708 PciI A'CATG_T Cuts at: 6961 6961 Size: 8062 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8062 PfoI T'CCnGG_A Cuts at: 5175 5175 Size: 8062 PmeI GTTT'AAAC Cuts at: 5366 5366 Size: 8062 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6131 6163 7644 7676 6131 Size: 32 1481 32 6517 Fragments arranged by size: 6517 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8062 PsiI TTA'TAA Cuts at: 5632 5632 Size: 8062 PspOMI G'GGCC_C Cuts at: 3277 3277 Size: 8062 PstI C_TGCA'G Cuts at: 830 2218 4497 5414 830 Size: 1388 2279 917 3478 Fragments arranged by size: 3478 2279 1388 917 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8062 PvuII CAG'CTG Cuts at: 2507 2507 Size: 8062 SacI G_AGCT'C Cuts at: 4159 5196 4159 Size: 1037 7025 SalI G'TCGA_C Cuts at: 1936 5404 1936 Size: 3468 4594 SapI GCTCTTCn'nnn_ Cuts at: 2664 2664 Size: 8062 SbfI CC_TGCA'GG Cuts at: 5414 5414 Size: 8062 ScaI AGT'ACT Cuts at: 6310 8022 6310 Size: 1712 6350 SfcI C'TryA_G Cuts at: 826 1045 2214 2319 4493 4623 5410 6545 7226 Size: 219 1169 105 2174 130 787 1135 681 Cuts at: 7226 7417 826 Size: 191 1471 Fragments arranged by size: 2174 1471 1169 1135 787 681 219 191 130 105 SfoI GGC'GCC Cuts at: 2033 2033 Size: 8062 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8062 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8062 SmaI CCC'GGG Cuts at: 1872 5389 1872 Size: 3517 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 2456 3830 5354 6166 7067 Size: 197 926 168 345 1374 1524 812 901 Cuts at: 7067 7329 820 Size: 262 1553 Fragments arranged by size: 1553 1524 1374 926 901 812 345 262 197 168 SnaBI TAC'GTA Cuts at: 493 5853 493 Size: 5360 2702 SpeI A'CTAG_T Cuts at: 152 4268 152 Size: 4116 3946 SphI G_CATG'C Cuts at: 1132 5420 1132 Size: 4288 3774 SspI AAT'ATT Cuts at: 5 52 3073 4121 4842 5136 5986 5 Size: 47 3021 1048 721 294 850 2081 Fragments arranged by size: 3021 2081 1048 850 721 294 47 StuI AGG'CCT Cuts at: 1517 3746 1517 Size: 2229 5833 StyI C'CwwG_G Cuts at: 513 1065 3570 5501 513 Size: 552 2505 1931 3074 Fragments arranged by size: 3074 2505 1931 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2861 3231 6089 6106 6259 6444 6889 Size: 1255 1281 370 2858 17 153 185 445 Cuts at: 6889 325 Size: 1498 Fragments arranged by size: 2858 1498 1281 1255 445 370 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3151 3245 6308 Size: 274 80 33 51 1443 1174 94 3063 Cuts at: 6308 8020 96 Size: 1712 138 Fragments arranged by size: 3063 1712 1443 1174 274 138 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8022 8054 8022 Size: 32 8030 Tth111I GACn'n_nGTC Cuts at: 1316 3725 1316 Size: 2409 5653 XbaI T'CTAG_A Cuts at: 5398 5398 Size: 8062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 8062 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8062 XmaI C'CCGG_G Cuts at: 1870 5387 1870 Size: 3517 4545 XmnI GAAnn'nnTTC Cuts at: 6191 6191 Size: 8062 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5870 276 Size: 53 83 186 936 4336 2468 Fragments arranged by size: 4336 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI