Restriction map for
pFN21AB7347
(Circular) (Six-base) MAPSORT of: pFN21AB7347.seq Check: 1739 from: 1 to: 8014
pFN21AB7347 8014 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3098 3098
Size: 8014
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4191 5824 278
Size: 53 83 186 936 2655 1633 2468
Fragments arranged by size:
2655 2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4767 5357 1937
Size: 2830 590 4594
Fragments arranged by size:
4594 2830 590
Acc65I G'GTAC_C
Cuts at: 3296 5335 3296
Size: 2039 5975
AclI AA'CG_TT
Cuts at: 1563 6141 6514 1563
Size: 4578 373 3063
Fragments arranged by size:
4578 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2976 3710 3869 4025 5159 6077 7461 7729
Size: 1344 734 159 156 1134 918 1384 268
Cuts at: 7729 1632
Size: 1917
Fragments arranged by size:
1917 1384 1344 1134 918 734 268 159
156
AfeI AGC'GCT
Cuts at: 2301 2774 2301
Size: 473 7541
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7817
AflIII A'CryG_T
Cuts at: 1203 4857 6913 7890 1203
Size: 3654 2056 977 1327
Fragments arranged by size:
3654 2056 1327 977
AgeI A'CCGG_T
Cuts at: 6845 6845
Size: 8014
AhdI GACnn_n'nnGTC
Cuts at: 6743 6743
Size: 8014
AleI CACnn'nnGTG
Cuts at: 1166 2726 1166
Size: 1560 6454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4955 5267 1184
Size: 3771 312 3931
Fragments arranged by size:
3931 3771 312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 7982
AlwNI CAG_nnn'CTG
Cuts at: 2581 4573 5757 7329 2581
Size: 1992 1184 1572 3266
Fragments arranged by size:
3266 1992 1572 1184
ApaI G_GGCC'C
Cuts at: 2101 2186 2626 2715 2917 2101
Size: 85 440 89 202 7198
Fragments arranged by size:
7198 440 202 89 85
ApaLI G'TGCA_C
Cuts at: 6071 7227 6071
Size: 1156 6858
ApoI r'AATT_y
Cuts at: 1493 3695 5323 5549 5701 7810 1493
Size: 2202 1628 226 152 2109 1697
Fragments arranged by size:
2202 2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6568 160
Size: 872 5536 1606
Fragments arranged by size:
5536 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8014
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2094 2211 2309 2625 2682 3273
Size: 73 102 49 117 98 316 57 591
Cuts at: 3273 3807 4262 4622 4807 4945 5101 5267 5339
Size: 534 455 360 185 138 156 166 72
Cuts at: 5339 1870
Size: 4545
Fragments arranged by size:
4545 591 534 455 360 316 185 166
156 138 117 102 98 73 72 57
49
AvrII C'CTAG_G
Cuts at: 3184 3184
Size: 8014
BamHI G'GATC_C
Cuts at: 3265 5344 5711 3265
Size: 2079 367 5568
Fragments arranged by size:
5568 2079 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2446 2480 3047 3296 3468
Size: 325 217 627 659 34 567 249 172
Cuts at: 3468 3557 3599 4330 5150 5335 6790 618
Size: 89 42 731 820 185 1455 1842
Fragments arranged by size:
1842 1455 820 731 659 627 567 325
249 217 185 172 89 42 34
BanII G_rGCy'C
Cuts at: 1391 2052 2074 2101 2186 2218 2333 2626 2664
Size: 661 22 27 85 32 115 293 38
Cuts at: 2664 2683 2715 2917 2989 3204 3281 3765 3881
Size: 19 32 202 72 215 77 484 116
Cuts at: 3881 4257 4292 4623 4698 5333 1391
Size: 376 35 331 75 635 4072
Fragments arranged by size:
4072 661 635 484 376 331 293 215
202 116 115 85 77 75 72 38
35 32 32 27 22 19
BbeI G_GCGC'C
Cuts at: 5154 5154
Size: 8014
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2818 3058 4448 4553 4607 928
Size: 971 919 240 1390 105 54 4335
Fragments arranged by size:
4335 1390 971 919 240 105 54
BbvCI CC'TCA_GC
Cuts at: 4590 4590
Size: 8014
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6205 6239 1903
Size: 34 4268 34 3678
Fragments arranged by size:
4268 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4355 5906 7116 4355
Size: 1551 1210 5253
Fragments arranged by size:
5253 1551 1210
BclI T'GATC_A
Cuts at: 1548 2730 1548
Size: 1182 6832
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 2277 2687
Size: 107 122 71 631 605 568 37 410
Cuts at: 2687 3452 6625 136
Size: 765 3173 1525
Fragments arranged by size:
3173 1525 765 631 605 568 410 122
107 71 37
BglII A'GATC_T
Cuts at: 8009 8009
Size: 8014
BlpI GC'TnA_GC
Cuts at: 5431 5431
Size: 8014
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2186 2451 2483 2626 2715 2917 3560
Size: 311 85 265 32 143 89 202 643
Cuts at: 3560 3604 4237 4889 6075 7231 1790
Size: 44 633 652 1186 1156 2573
Fragments arranged by size:
2573 1186 1156 652 643 633 311 265
202 143 89 85 44 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5745 6703 442
Size: 416 53 83 399 24 4328 958 1753
Fragments arranged by size:
4328 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8014
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 4311 4501 3025
Size: 1286 190 6538
Fragments arranged by size:
6538 1286 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2697 2804 3407 3740 4181 4271 4496 4517
Size: 1458 107 603 333 441 90 225 21
Cuts at: 4517 4550 5306 6674 1239
Size: 33 756 1368 2579
Fragments arranged by size:
2579 1458 1368 756 603 441 333 225
107 90 33 21
Bpu10I CC'TnA_GC
Cuts at: 4145 4590 4145
Size: 445 7569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2973 4028 5089 6139 7004 7302 2973
Size: 1055 1061 1050 865 298 3685
Fragments arranged by size:
3685 1061 1055 1050 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3037 3231 3499 3862 6677 882
Size: 617 1538 194 268 363 2815 2219
Fragments arranged by size:
2815 2219 1538 617 363 268 194
BsaAI yAC'GTr
Cuts at: 493 1204 5062 5805 493
Size: 711 3858 743 2702
Fragments arranged by size:
3858 2702 743 711
BsaBI GATnn'nnATC
Cuts at: 8008 8008
Size: 8014
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2324 4188 4791 5151
Size: 53 83 186 936 791 1864 603 360
Cuts at: 5151 5821 6203 6838 275
Size: 670 382 635 1451
Fragments arranged by size:
1864 1451 936 791 670 635 603 382
360 186 83 53
BsaWI w'CCGG_w
Cuts at: 2038 2383 3125 4096 6447 6845 7119 7266 7677
Size: 345 742 971 2351 398 274 147 411
Cuts at: 7677 2038
Size: 2375
Fragments arranged by size:
2375 2351 971 742 411 398 345 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3120 3150 3666 3696 4610
Size: 30 165 30 1717 30 516 30 914
Cuts at: 4610 4640 1178
Size: 30 4552
Fragments arranged by size:
4552 1717 914 516 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 2810 3204 3212 3884 4538 1186
Size: 1594 30 394 8 672 654 4662
Fragments arranged by size:
4662 1594 672 654 394 30 8
BseYI C'CCAG_C
Cuts at: 2335 2544 2755 2800 2901 3440 3632 3727 4153
Size: 209 211 45 101 539 192 95 426
Cuts at: 4153 4365 4818 7217 7713 2335
Size: 212 453 2399 496 2636
Fragments arranged by size:
2636 2399 539 496 453 426 212 211
209 192 101 95 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3853 3959 4295 4925 1163
Size: 573 2117 106 336 630 4252
Fragments arranged by size:
4252 2117 630 573 336 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5300 5464 6225 7253 1540
Size: 275 186 3299 164 761 1028 2301
Fragments arranged by size:
3299 2301 1028 761 275 186 164
BsiHKAI G_wGCw'C
Cuts at: 2931 3765 3867 4257 5075 5095 6075 6160 7231
Size: 834 102 390 818 20 980 85 1071
Cuts at: 7231 2931
Size: 3714
Fragments arranged by size:
3714 1071 980 834 818 390 102 85
20
BsmI GAATG_Cn'
Cuts at: 2537 5525 5618 2537
Size: 2988 93 4933
Fragments arranged by size:
4933 2988 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3186 3186
Size: 8014
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2074 2101 2186 2218 2333 2451
Size: 399 262 22 27 85 32 115 118
Cuts at: 2451 2483 2626 2664 2683 2715 2917 2931 2989
Size: 32 143 38 19 32 202 14 58
Cuts at: 2989 3204 3281 3560 3604 3765 3867 3881 4237
Size: 215 77 279 44 161 102 14 356
Cuts at: 4237 4257 4292 4623 4698 4889 5075 5095 5333
Size: 20 35 331 75 191 186 20 238
Cuts at: 5333 6075 6160 7231 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 331 279 262
238 215 202 191 186 161 143 118
115 102 85 85 77 75 58 44
38 35 32 32 32 27 22 20
20 19 14 14
BspEI T'CCGG_A
Cuts at: 2038 3125 7677 2038
Size: 1087 4552 2375
Fragments arranged by size:
4552 2375 1087
BspHI T'CATG_A
Cuts at: 5903 7633 5903
Size: 1730 6284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3019 3098 3151 4722 5226 5369 844
Size: 2175 79 53 1571 504 143 3489
Fragments arranged by size:
3489 2175 1571 504 143 79 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 2116 4112 5272 5901 1394
Size: 429 204 89 1996 1160 629 3507
Fragments arranged by size:
3507 1996 1160 629 429 204 89
BsrDI GCAATG_nn'
Cuts at: 65 1248 6509 6683 65
Size: 1183 5261 174 1396
Fragments arranged by size:
5261 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2403 3240 3293 3471 4181 4554 6658
Size: 110 593 837 53 178 710 373 2104
Cuts at: 6658 6845 1700
Size: 187 2869
Fragments arranged by size:
2869 2104 837 710 593 373 187 178
110 53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8014
BssHII G'CGCG_C
Cuts at: 1925 2549 1925
Size: 624 7390
BssSI C'ACGA_G
Cuts at: 2375 3346 6074 7086 2375
Size: 971 2728 1012 3303
Fragments arranged by size:
3303 2728 1012 971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2749 4282 794
Size: 1955 1533 4526
Fragments arranged by size:
4526 1955 1533
BstBI TT'CG_AA
Cuts at: 6818 6818
Size: 8014
BstEII G'GTnAC_C
Cuts at: 1184 2563 1184
Size: 1379 6635
BstXI CCAn_nnnn'nTGG
Cuts at: 3551 4826 3551
Size: 1275 6739
BstYI r'GATC_y
Cuts at: 1972 3265 4501 5259 5344 5711 6096 6113 7565
Size: 1293 1236 758 85 367 385 17 1452
Cuts at: 7565 7651 7663 8009 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1293 1236 758 385 367 346
86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2693 4911 2693
Size: 2218 5796
BtgI C'CryG_G
Cuts at: 513 1065 2484 2508 2610 2655 3522 4057 4063
Size: 552 1419 24 102 45 867 535 6
Cuts at: 4063 4320 4395 4881 4985 5239 513
Size: 257 75 486 104 254 3288
Fragments arranged by size:
3288 1419 867 552 535 486 257 254
104 102 75 45 24 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7275
Fragments arranged by size:
7275 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 3865 4237 4367 5530 6324 6344 2733
Size: 1132 372 130 1163 794 20 4403
Fragments arranged by size:
4403 1163 1132 794 372 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3066 3101 529
Size: 35 2502 35 5442
Fragments arranged by size:
5442 2502 35 35
DraI TTT'AAA
Cuts at: 5318 5318
Size: 8014
DraIII CAC_nnn'GTG
Cuts at: 2749 2749
Size: 8014
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7021 809
Size: 507 5705 1802
Fragments arranged by size:
5705 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2241 2405
Size: 54 1425 50 275 18 399 12 164
Cuts at: 2405 2880 4060 4179 4200 5297 5461 6350 7979
Size: 475 1180 119 21 1097 164 889 1629
Cuts at: 7979 8
Size: 43
Fragments arranged by size:
1629 1425 1180 1097 889 475 399 275
164 164 119 54 50 43 21 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 5297 5461 1537
Size: 275 3485 164 4090
Fragments arranged by size:
4090 3485 275 164
EarI CTCTTCn'nnn_
Cuts at: 1353 3888 5944 1353
Size: 2535 2056 3423
Fragments arranged by size:
3423 2535 2056
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2146 2489 3280 3713 6601 6975
Size: 1064 138 557 343 791 433 2888 374
Cuts at: 6975 7121 387
Size: 146 1280
Fragments arranged by size:
2888 1280 1064 791 557 433 374 343
146 138
EcoICRI GAG'CTC
Cuts at: 3763 4255 3763
Size: 492 7522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 2804 2976 3407 3710 3740 3869
Size: 393 1065 107 172 431 303 30 129
Cuts at: 3869 4025 4181 4271 4496 4517 4550 5159 5306
Size: 156 156 90 225 21 33 609 147
Cuts at: 5306 6077 6674 7461 7729 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 1065 787 771 609 597 431 393
303 268 225 172 156 156 147 129
107 90 33 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 2783 3836 3956 2696
Size: 87 1053 120 6754
Fragments arranged by size:
6754 1053 120 87
EcoO109I rG'GnC_Cy
Cuts at: 2098 2176 2183 2578 2622 2689 2913 3163 3181
Size: 78 7 395 44 67 224 250 18
Cuts at: 3181 3444 3821 3889 4150 2098
Size: 263 377 68 261 5962
Fragments arranged by size:
5962 395 377 263 261 250 224 78
68 67 44 18 7
EcoRI G'AATT_C
Cuts at: 5323 5701 5323
Size: 378 7636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 8014
FspI TGC'GCA
Cuts at: 2981 3159 4925 6520 2981
Size: 178 1766 1595 4475
Fragments arranged by size:
4475 1766 1595 178
FspAI rTGC'GCAy
Cuts at: 4925 4925
Size: 8014
HaeII r_GCGC'y
Cuts at: 1678 2303 2776 2998 4168 5154 7161 1678
Size: 625 473 222 1170 986 2007 2531
Fragments arranged by size:
2531 2007 1170 986 625 473 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4026 4058 4561 4593 6655
Size: 32 135 32 2454 32 503 32 2062
Cuts at: 6655 6687 6729 6761 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2454 2062 503 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5014 5358 5604 669
Size: 988 281 3076 344 246 3079
Fragments arranged by size:
3079 3076 988 344 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8014
HpaI GTT'AAC
Cuts at: 5604 5604
Size: 8014
KasI G'GCGC_C
Cuts at: 5150 5150
Size: 8014
KpnI G_GTAC'C
Cuts at: 3300 5339 3300
Size: 2039 5975
MfeI C'AATT_G
Cuts at: 5613 5613
Size: 8014
MluI A'CGCG_T
Cuts at: 7890 7890
Size: 8014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7128 7312 1305
Size: 5823 184 2007
Fragments arranged by size:
5823 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4202 10
Size: 54 1425 343 2370 3822
Fragments arranged by size:
3822 2370 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 2738 3257 3488 3549 4208 5060
Size: 648 1560 12 519 231 61 659 852
Cuts at: 5060 5972 6331 6490 518
Size: 912 359 159 2042
Fragments arranged by size:
2042 1560 912 852 659 648 519 359
231 159 61 12
MspA1I CmG'CkG
Cuts at: 1606 2510 3134 3627 4123 4822 4987 5430 6107
Size: 904 624 493 496 699 165 443 677
Cuts at: 6107 7255 7500 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 904 699 677 624 496 493
443 245 165
NaeI GCC'GGC
Cuts at: 2405 4183 2405
Size: 1778 6236
NarI GG'CG_CC
Cuts at: 5151 5151
Size: 8014
NcoI C'CATG_G
Cuts at: 513 1065 2655 4320 4395 513
Size: 552 1590 1665 75 4132
Fragments arranged by size:
4132 1665 1590 552 75
NdeI CA'TA_TG
Cuts at: 387 4905 387
Size: 4518 3496
NgoMIV G'CCGG_C
Cuts at: 2403 4181 2403
Size: 1778 6236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8014
NotI GC'GGCC_GC
Cuts at: 5461 5461
Size: 8014
NspI r_CATG'y
Cuts at: 1132 1229 3262 3487 3613 4861 4900 5372 6917
Size: 97 2033 225 126 1248 39 472 1545
Cuts at: 6917 1132
Size: 2229
Fragments arranged by size:
2229 2033 1545 1248 472 225 126 97
39
PasI CC'CwG_GG
Cuts at: 1348 2187 2451 2715 3577 3701 1348
Size: 839 264 264 862 124 5661
Fragments arranged by size:
5661 862 839 264 264 124
PciI A'CATG_T
Cuts at: 4857 6913 4857
Size: 2056 5958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3044 3643 1108
Size: 1936 599 5479
Fragments arranged by size:
5479 1936 599
PfoI T'CCnGG_A
Cuts at: 2781 3150 3756 4509 2781
Size: 369 606 753 6286
Fragments arranged by size:
6286 753 606 369
PmeI GTTT'AAAC
Cuts at: 5318 5318
Size: 8014
PmlI CAC'GTG
Cuts at: 5062 5062
Size: 8014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6083 6115 7596 7628 6083
Size: 32 1481 32 6469
Fragments arranged by size:
6469 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2578 3163 3181 3889 4150 2176
Size: 402 585 18 708 261 6040
Fragments arranged by size:
6040 708 585 402 261 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8014
PsiI TTA'TAA
Cuts at: 5584 5584
Size: 8014
PspOMI G'GGCC_C
Cuts at: 2097 2182 2622 2711 2913 2097
Size: 85 440 89 202 7198
Fragments arranged by size:
7198 440 202 89 85
PstI C_TGCA'G
Cuts at: 830 2142 3027 5256 5366 830
Size: 1312 885 2229 110 3478
Fragments arranged by size:
3478 2229 1312 885 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8014
PvuII CAG'CTG
Cuts at: 3134 4123 3134
Size: 989 7025
SacI G_AGCT'C
Cuts at: 3765 4257 3765
Size: 492 7522
SacII CC_GC'GG
Cuts at: 2511 4988 2511
Size: 2477 5537
SalI G'TCGA_C
Cuts at: 1936 5356 1936
Size: 3420 4594
SanDI GG'GwC_CC
Cuts at: 4150 4150
Size: 8014
SbfI CC_TGCA'GG
Cuts at: 5256 5366 5256
Size: 110 7904
ScaI AGT'ACT
Cuts at: 4484 4628 6262 7974 4484
Size: 144 1634 1712 4524
Fragments arranged by size:
4524 1712 1634 144
SexAI A'CCwGG_T
Cuts at: 5109 5109
Size: 8014
SfcI C'TryA_G
Cuts at: 826 1045 2138 3023 5252 5362 6497 7178 7369
Size: 219 1093 885 2229 110 1135 681 191
Cuts at: 7369 826
Size: 1471
Fragments arranged by size:
2229 1471 1135 1093 885 681 219 191
110
SfiI GGCCn_nnn'nGGCC
Cuts at: 3452 3452
Size: 8014
SfoI GGC'GCC
Cuts at: 5152 5152
Size: 8014
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8014
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8014
SmaI CCC'GGG
Cuts at: 1872 2047 2096 2627 2684 3275 5341 1872
Size: 175 49 531 57 591 2066 4545
Fragments arranged by size:
4545 2066 591 531 175 57 49
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2988 4043 5068 6118 7019 7281
Size: 197 926 1045 1055 1025 1050 901 262
Cuts at: 7281 820
Size: 1553
Fragments arranged by size:
1553 1055 1050 1045 1025 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 5805 493
Size: 5312 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8014
SphI G_CATG'C
Cuts at: 1132 4900 5372 1132
Size: 3768 472 3774
Fragments arranged by size:
3774 3768 472
SspI AAT'ATT
Cuts at: 5 52 5938 5
Size: 47 5886 2081
Fragments arranged by size:
5886 2081 47
StuI AGG'CCT
Cuts at: 1517 2876 4595 4938 1517
Size: 1359 1719 343 4593
Fragments arranged by size:
4593 1719 1359 343
StyI C'CwwG_G
Cuts at: 513 1065 2655 2664 2877 3184 4311 4320 4395
Size: 552 1590 9 213 307 1127 9 75
Cuts at: 4395 5453 513
Size: 1058 3074
Fragments arranged by size:
3074 1590 1127 1058 552 307 213 75
9 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2575 2747 2825 3109 3561 3587 6041
Size: 1255 995 172 78 284 452 26 2454
Cuts at: 6041 6058 6211 6396 6841 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 995 452 445 284 185
172 153 78 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4482 4626 4647
Size: 274 80 33 51 1443 2505 144 21
Cuts at: 4647 6260 7972 96
Size: 1613 1712 138
Fragments arranged by size:
2505 1712 1613 1443 274 144 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3120 3152 4109 4141 4854 4886 5074 5106 7974
Size: 32 957 32 713 32 188 32 2868
Cuts at: 7974 8006 3120
Size: 32 3128
Fragments arranged by size:
3128 2868 957 713 188 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3062 5009 1316
Size: 1746 1947 4321
Fragments arranged by size:
4321 1947 1746
XbaI T'CTAG_A
Cuts at: 5350 5350
Size: 8014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2662 3259 3640 4705 4861 2662
Size: 597 381 1065 156 5815
Fragments arranged by size:
5815 1065 597 381 156
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8014
XmaI C'CCGG_G
Cuts at: 1870 2045 2094 2625 2682 3273 5339 1870
Size: 175 49 531 57 591 2066 4545
Fragments arranged by size:
4545 2066 591 531 175 57 49
XmnI GAAnn'nnTTC
Cuts at: 3695 6143 3695
Size: 2448 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4189 5822 276
Size: 53 83 186 936 2655 1633 2468
Fragments arranged by size:
2655 2468 1633 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI
NsiI PacI PspXI PsrI RsrII SapI SrfI SwaI