Restriction map for
pFN21AB7344
(Circular) (Six-base) MAPSORT of: pFN21AB7344.seq Check: 3907 from: 1 to: 7852
pFN21AB7344 7852 bp
With 182 enzymes: SgfI *
September 8, 2016 14:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5662 278
Size: 53 83 186 936 4126 2468
Fragments arranged by size:
4126 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3250 4717 5195 1937
Size: 1313 1467 478 4594
Fragments arranged by size:
4594 1467 1313 478
Acc65I G'GTAC_C
Cuts at: 5173 5173
Size: 7852
AclI AA'CG_TT
Cuts at: 1563 5979 6352 1563
Size: 4416 373 3063
Fragments arranged by size:
4416 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2005 2598 3975 4565 5915 7299 7567 1632
Size: 373 593 1377 590 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1377 1350 593 590 373 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7655
AflIII A'CryG_T
Cuts at: 1203 6751 7728 1203
Size: 5548 977 1327
Fragments arranged by size:
5548 1327 977
AgeI A'CCGG_T
Cuts at: 6683 6683
Size: 7852
AhdI GACnn_n'nnGTC
Cuts at: 6581 6581
Size: 7852
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3944 1184
Size: 2760 5092
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 7820
AlwNI CAG_nnn'CTG
Cuts at: 3037 5595 7167 3037
Size: 2558 1572 3722
Fragments arranged by size:
3722 2558 1572
ApaLI G'TGCA_C
Cuts at: 4556 5909 7065 4556
Size: 1353 1156 5343
Fragments arranged by size:
5343 1353 1156
ApoI r'AATT_y
Cuts at: 1493 2075 2220 2509 2655 2835 2854 2860 2991
Size: 582 145 289 146 180 19 6 131
Cuts at: 2991 3071 4039 4078 4265 5161 5387 5539 7648
Size: 80 968 39 187 896 226 152 2109
Cuts at: 7648 1493
Size: 1697
Fragments arranged by size:
2109 1697 968 896 582 289 226 187
180 152 146 145 131 80 39 19
6
AseI AT'TA_AT
Cuts at: 160 1032 2310 2331 3441 6406 160
Size: 872 1278 21 1110 2965 1606
Fragments arranged by size:
2965 1606 1278 1110 872 21
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7852
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 4450 4826 5177 1870
Size: 73 924 1583 376 351 4545
Fragments arranged by size:
4545 1583 924 376 351 73
AvrII C'CTAG_G
Cuts at: 4291 4291
Size: 7852
BamHI G'GATC_C
Cuts at: 5182 5549 5182
Size: 367 7485
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 4901 4946 4970 5173
Size: 325 217 627 327 2787 45 24 203
Cuts at: 5173 6628 618
Size: 1455 1842
Fragments arranged by size:
2787 1842 1455 627 327 325 217 203
45 24
BanII G_rGCy'C
Cuts at: 1391 2392 2433 4377 5171 1391
Size: 1001 41 1944 794 4072
Fragments arranged by size:
4072 1944 1001 794 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 2811 4233 4746 928
Size: 971 297 615 1422 513 4034
Fragments arranged by size:
4034 1422 971 615 513 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6043 6077 1903
Size: 34 4106 34 3678
Fragments arranged by size:
4106 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4243 5744 6954 4243
Size: 1501 1210 5141
Fragments arranged by size:
5141 1501 1210
BclI T'GATC_A
Cuts at: 1548 2281 1548
Size: 733 7119
BfrBI ATG'CAT
Cuts at: 3882 3882
Size: 7852
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4944 6463 136
Size: 107 122 71 631 605 3272 1519 1525
Fragments arranged by size:
3272 1525 1519 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3934 7847 3934
Size: 3913 3939
BlpI GC'TnA_GC
Cuts at: 5269 5269
Size: 7852
Bme1580I G_kGCm'C
Cuts at: 1790 4560 4975 5913 7069 1790
Size: 2770 415 938 1156 2573
Fragments arranged by size:
2770 2573 1156 938 415
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7852
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3146 4917 5583
Size: 416 53 83 399 24 1729 1771 666
Cuts at: 5583 6541 442
Size: 958 1753
Fragments arranged by size:
1771 1753 1729 958 666 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3305 1056
Size: 2249 5603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2289 2451 2670 6512 1239
Size: 793 257 162 219 3842 2579
Fragments arranged by size:
3842 2579 793 257 219 162
Bpu10I CC'TnA_GC
Cuts at: 4473 5012 4473
Size: 539 7313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5977 6842 7140 5977
Size: 865 298 6689
Fragments arranged by size:
6689 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6515 882
Size: 617 5016 2219
Fragments arranged by size:
5016 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5643 493
Size: 711 4439 2702
Fragments arranged by size:
4439 2702 711
BsaBI GATnn'nnATC
Cuts at: 3411 7846 3411
Size: 4435 3417
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4138 5659 6041 6676
Size: 53 83 186 936 2605 1521 382 635
Cuts at: 6676 275
Size: 1451
Fragments arranged by size:
2605 1521 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6285 6683 6957 7104 7515 6285
Size: 398 274 147 411 6622
Fragments arranged by size:
6622 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3210 3240 1178
Size: 30 165 30 1807 30 5790
Fragments arranged by size:
5790 1807 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3653 3723 3957 3960 5065 1186
Size: 2467 70 234 3 1105 3973
Fragments arranged by size:
3973 2467 1105 234 70 3
BseYI C'CCAG_C
Cuts at: 4961 7055 7551 4961
Size: 2094 496 5262
Fragments arranged by size:
5262 2094 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3762 1163
Size: 573 2026 5253
Fragments arranged by size:
5253 2026 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5302 6063 7091 1540
Size: 275 186 3301 761 1028 2301
Fragments arranged by size:
3301 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2392 2433 3733 4442 4560 5913 5998 7069 2392
Size: 41 1300 709 118 1353 85 1071 3175
Fragments arranged by size:
3175 1353 1300 1071 709 118 85 41
BsmI GAATG_Cn'
Cuts at: 2128 3884 4002 5363 5456 2128
Size: 1756 118 1361 93 4524
Fragments arranged by size:
4524 1756 1361 118 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2433 3733 4377 4442 4560 4975
Size: 399 602 41 1300 644 65 118 415
Cuts at: 4975 5171 5913 5998 7069 1391
Size: 196 742 85 1071 2174
Fragments arranged by size:
2174 1300 1071 742 644 602 415 399
196 118 85 65 41
BspEI T'CCGG_A
Cuts at: 7515 7515
Size: 7852
BspHI T'CATG_A
Cuts at: 5741 7471 5741
Size: 1730 6122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3306 5207 844
Size: 2462 1901 3489
Fragments arranged by size:
3489 2462 1901
BsrBI CCG'CTC
Cuts at: 1394 1823 4968 5739 1394
Size: 429 3145 771 3507
Fragments arranged by size:
3507 3145 771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2040 6347 6521 65
Size: 1183 792 4307 174 1396
Fragments arranged by size:
4307 1396 1183 792 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6496 6683 1700
Size: 110 4686 187 2869
Fragments arranged by size:
4686 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3634 96
Size: 3538 4314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7852
BssSI C'ACGA_G
Cuts at: 5912 6924 5912
Size: 1012 6840
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3037 794
Size: 2243 5609
BstBI TT'CG_AA
Cuts at: 2659 6656 2659
Size: 3997 3855
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7852
BstXI CCAn_nnnn'nTGG
Cuts at: 5084 5084
Size: 7852
BstYI r'GATC_y
Cuts at: 1972 2298 3934 5182 5549 5934 5951 7403 7489
Size: 326 1636 1248 367 385 17 1452 86
Cuts at: 7489 7501 7847 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1636 1452 1248 385 367 346 326
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4362 4362
Size: 7852
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6361
Fragments arranged by size:
6361 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7113
Fragments arranged by size:
7113 683 56
BtsI GCAGTG_nn'
Cuts at: 4939 5368 6162 6182 4939
Size: 429 794 20 6609
Fragments arranged by size:
6609 794 429 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7852
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7817
DraI TTT'AAA
Cuts at: 2997 5156 2997
Size: 2159 5693
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6859 809
Size: 507 5543 1802
Fragments arranged by size:
5543 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2695 4328 5041
Size: 54 1425 50 275 18 865 1633 713
Cuts at: 5041 5299 6188 7817 8
Size: 258 889 1629 43
Fragments arranged by size:
1633 1629 1425 889 865 713 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5299 1537
Size: 275 3487 4090
Fragments arranged by size:
4090 3487 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 5782 1353
Size: 1536 2893 3423
Fragments arranged by size:
3423 2893 1536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 6439 6813 6959 387
Size: 1064 138 3491 1359 374 146 1280
Fragments arranged by size:
3491 1359 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2390 2431 2390
Size: 41 7811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2032 2289 2451 2598 2670 3975
Size: 393 373 27 257 162 147 72 1305
Cuts at: 3975 4565 5915 6512 7299 7567 1239
Size: 590 1350 597 787 268 1524
Fragments arranged by size:
1524 1350 1305 787 597 590 393 373
268 257 162 147 72 27
EcoNI CCTnn'n_nnAGG
Cuts at: 4361 4361
Size: 7852
EcoO109I rG'GnC_Cy
Cuts at: 4259 4454 4259
Size: 195 7657
EcoRI G'AATT_C
Cuts at: 2075 5161 5539 2075
Size: 3086 378 4388
Fragments arranged by size:
4388 3086 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3552 3552
Size: 7852
FspI TGC'GCA
Cuts at: 6358 6358
Size: 7852
HaeII r_GCGC'y
Cuts at: 1678 4629 6999 1678
Size: 2951 2370 2531
Fragments arranged by size:
2951 2531 2370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 3608 3640 3651
Size: 32 135 32 1163 32 841 32 11
Cuts at: 3651 3666 3683 3698 4429 4461 4723 4755 4795
Size: 15 17 15 731 32 262 32 40
Cuts at: 4795 4827 6493 6525 6567 6599 1373
Size: 32 1666 32 42 32 2626
Fragments arranged by size:
2626 1666 1163 841 731 262 135 42
40 32 32 32 32 32 32 32
32 32 17 15 15 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 5196 5442 669
Size: 988 281 130 3128 246 3079
Fragments arranged by size:
3128 3079 988 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7852
HpaI GTT'AAC
Cuts at: 2068 5442 2068
Size: 3374 4478
KpnI G_GTAC'C
Cuts at: 5177 5177
Size: 7852
MfeI C'AATT_G
Cuts at: 5451 5451
Size: 7852
MluI A'CGCG_T
Cuts at: 7728 7728
Size: 7852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4810 6966 7150 1305
Size: 3505 2156 184 2007
Fragments arranged by size:
3505 2156 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4330 5043 10
Size: 54 1425 343 2498 713 2819
Fragments arranged by size:
2819 2498 1425 713 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4952 5810 6169 6328 518
Size: 648 3786 858 359 159 2042
Fragments arranged by size:
3786 2042 858 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2756 3293 3746 4917 5268 5945 7093 7338
Size: 1150 537 453 1171 351 677 1148 245
Cuts at: 7338 1606
Size: 2120
Fragments arranged by size:
2120 1171 1150 1148 677 537 453 351
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6361
Fragments arranged by size:
6361 939 552
NdeI CA'TA_TG
Cuts at: 387 2293 3178 4111 387
Size: 1906 885 933 4128
Fragments arranged by size:
4128 1906 933 885
NheI G'CTAG_C
Cuts at: 1052 3301 1052
Size: 2249 5603
NotI GC'GGCC_GC
Cuts at: 5299 5299
Size: 7852
NsiI A_TGCA'T
Cuts at: 3884 3884
Size: 7852
NspI r_CATG'y
Cuts at: 1132 1229 2752 5210 6755 1132
Size: 97 1523 2458 1545 2229
Fragments arranged by size:
2458 2229 1545 1523 97
PasI CC'CwG_GG
Cuts at: 1348 3822 4255 1348
Size: 2474 433 4945
Fragments arranged by size:
4945 2474 433
PciI A'CATG_T
Cuts at: 6751 6751
Size: 7852
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2124 2294 4622 1108
Size: 902 114 170 2328 4338
Fragments arranged by size:
4338 2328 902 170 114
PmeI GTTT'AAAC
Cuts at: 5156 5156
Size: 7852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5921 5953 7434 7466 5921
Size: 32 1481 32 6307
Fragments arranged by size:
6307 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4259 4454 4259
Size: 195 7657
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7852
PsiI TTA'TAA
Cuts at: 2100 2600 5422 2100
Size: 500 2822 4530
Fragments arranged by size:
4530 2822 500
PstI C_TGCA'G
Cuts at: 830 5204 830
Size: 4374 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7852
PvuII CAG'CTG
Cuts at: 3293 3746 3293
Size: 453 7399
SacI G_AGCT'C
Cuts at: 2392 2433 2392
Size: 41 7811
SalI G'TCGA_C
Cuts at: 1936 5194 1936
Size: 3258 4594
SbfI CC_TGCA'GG
Cuts at: 5204 5204
Size: 7852
ScaI AGT'ACT
Cuts at: 3532 6100 7812 3532
Size: 2568 1712 3572
Fragments arranged by size:
3572 2568 1712
SexAI A'CCwGG_T
Cuts at: 4456 4456
Size: 7852
SfcI C'TryA_G
Cuts at: 826 1045 3402 5200 6335 7016 7207 826
Size: 219 2357 1798 1135 681 191 1471
Fragments arranged by size:
2357 1798 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7852
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7852
SmaI CCC'GGG
Cuts at: 1872 5179 1872
Size: 3307 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 5956 6857 7119 820
Size: 197 926 924 3089 901 262 1553
Fragments arranged by size:
3089 1553 926 924 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5643 493
Size: 5150 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7852
SphI G_CATG'C
Cuts at: 1132 5210 1132
Size: 4078 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5776 5
Size: 47 3393 2331 2081
Fragments arranged by size:
3393 2331 2081 47
StuI AGG'CCT
Cuts at: 1517 2422 1517
Size: 905 6947
StyI C'CwwG_G
Cuts at: 513 1065 2004 3351 3385 4291 5291 513
Size: 552 939 1347 34 906 1000 3074
Fragments arranged by size:
3074 1347 1000 939 906 552 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2742 4407 4532 4946 5879 5896 6049
Size: 1255 1162 1665 125 414 933 17 153
Cuts at: 6049 6234 6679 325
Size: 185 445 1498
Fragments arranged by size:
1665 1498 1255 1162 933 445 414 185
153 125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3360 3530 3634
Size: 274 80 33 51 1443 1383 170 104
Cuts at: 3634 4462 6098 7810 96
Size: 828 1636 1712 138
Fragments arranged by size:
1712 1636 1443 1383 828 274 170 138
104 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2750 2782 4903 4935 7812 7844 2750
Size: 32 2121 32 2877 32 2758
Fragments arranged by size:
2877 2758 2121 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4519 1316
Size: 3203 4649
XbaI T'CTAG_A
Cuts at: 2425 2677 5188 2425
Size: 252 2511 5089
Fragments arranged by size:
5089 2511 252
XhoI C'TCGA_G
Cuts at: 1943 2867 1943
Size: 924 6928
XmaI C'CCGG_G
Cuts at: 1870 5177 1870
Size: 3307 4545
XmnI GAAnn'nnTTC
Cuts at: 4039 5981 4039
Size: 1942 5910
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5660 276
Size: 53 83 186 936 4126 2468
Fragments arranged by size:
4126 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI