Restriction map for
pFN21AB7342
(Circular) (Six-base) MAPSORT of: pFN21AB7342.seq Check: 9532 from: 1 to: 7387
pFN21AB7342 7387 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5197 278
Size: 53 83 186 936 3661 2468
Fragments arranged by size:
3661 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3177 4730 1937
Size: 1240 1553 4594
Fragments arranged by size:
4594 1553 1240
Acc65I G'GTAC_C
Cuts at: 4708 4708
Size: 7387
AclI AA'CG_TT
Cuts at: 1563 5514 5887 1563
Size: 3951 373 3063
Fragments arranged by size:
3951 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2194 2766 4239 5450 6834 7102 1632
Size: 479 83 572 1473 1211 1384 268 1917
Fragments arranged by size:
1917 1473 1384 1211 572 479 268 83
AflII C'TTAA_G
Cuts at: 820 1017 3520 820
Size: 197 2503 4687
Fragments arranged by size:
4687 2503 197
AflIII A'CryG_T
Cuts at: 1203 2464 4133 6286 7263 1203
Size: 1261 1669 2153 977 1327
Fragments arranged by size:
2153 1669 1327 1261 977
AgeI A'CCGG_T
Cuts at: 2903 6218 2903
Size: 3315 4072
AhdI GACnn_n'nnGTC
Cuts at: 3753 6116 3753
Size: 2363 5024
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4690 1184
Size: 3506 3881
AlwNI CAG_nnn'CTG
Cuts at: 5130 6702 5130
Size: 1572 5815
ApaI G_GGCC'C
Cuts at: 3410 3410
Size: 7387
ApaLI G'TGCA_C
Cuts at: 3364 5444 6600 3364
Size: 2080 1156 4151
Fragments arranged by size:
4151 2080 1156
ApoI r'AATT_y
Cuts at: 1493 2214 3617 4198 4652 4696 4922 5074 7183
Size: 721 1403 581 454 44 226 152 2109
Cuts at: 7183 1493
Size: 1697
Fragments arranged by size:
2109 1697 1403 721 581 454 226 152
44
AseI AT'TA_AT
Cuts at: 160 1032 3564 5941 160
Size: 872 2532 2377 1606
Fragments arranged by size:
2532 2377 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
AvaI C'yCGr_G
Cuts at: 1870 1943 4712 1870
Size: 73 2769 4545
Fragments arranged by size:
4545 2769 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2892 2925 2892
Size: 33 7354
BamHI G'GATC_C
Cuts at: 4717 5084 4717
Size: 367 7020
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4708 6163 618
Size: 325 217 627 2921 1455 1842
Fragments arranged by size:
2921 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2416 3347 3410 4706 1391
Size: 1025 931 63 1296 4072
Fragments arranged by size:
4072 1296 1025 931 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 4188 4542 928
Size: 971 354 1935 354 3773
Fragments arranged by size:
3773 1935 971 354 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2609 2643 5578 5612 1903
Size: 34 672 34 2935 34 3678
Fragments arranged by size:
3678 2935 672 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 5279 6489 3473
Size: 1806 1210 4371
Fragments arranged by size:
4371 1806 1210
BclI T'GATC_A
Cuts at: 1548 2688 1548
Size: 1140 6247
BfrBI ATG'CAT
Cuts at: 4677 4677
Size: 7387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5998 136
Size: 107 122 71 631 605 4326 1525
Fragments arranged by size:
4326 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7382 7382
Size: 7387
BlpI GC'TnA_GC
Cuts at: 4804 4804
Size: 7387
Bme1580I G_kGCm'C
Cuts at: 1790 3368 3410 5448 6604 1790
Size: 1578 42 2038 1156 2573
Fragments arranged by size:
2573 2038 1578 1156 42
BmgBI CAC'GTC
Cuts at: 1318 3112 1318
Size: 1794 5593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 5118 6076
Size: 416 53 83 399 24 1169 2532 958
Cuts at: 6076 442
Size: 1753
Fragments arranged by size:
2532 1753 1169 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 3408 4248 4646 6047 1239
Size: 941 1228 840 398 1401 2579
Fragments arranged by size:
2579 1401 1228 941 840 398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2375 3056 3604 5512 6377 6675 2156
Size: 219 681 548 1908 865 298 2868
Fragments arranged by size:
2868 1908 865 681 548 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6050 882
Size: 617 4551 2219
Fragments arranged by size:
4551 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5178 493
Size: 711 3974 2702
Fragments arranged by size:
3974 2702 711
BsaBI GATnn'nnATC
Cuts at: 7381 7381
Size: 7387
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5194 5576 6211 275
Size: 53 83 186 936 3661 382 635 1451
Fragments arranged by size:
3661 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2190 2774 2903 5820 6218 6492 6639 7050 2190
Size: 584 129 2917 398 274 147 411 2527
Fragments arranged by size:
2917 2527 584 411 398 274 147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 4221 4251 1178
Size: 30 165 30 1026 30 1762 30 4314
Fragments arranged by size:
4314 1762 1026 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 3150 4532 1186
Size: 991 973 1382 4041
Fragments arranged by size:
4041 1382 991 973
BseYI C'CCAG_C
Cuts at: 3457 4191 6590 7086 3457
Size: 734 2399 496 3758
Fragments arranged by size:
3758 2399 734 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2475 1163
Size: 573 739 6075
Fragments arranged by size:
6075 739 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4606 4837 5598 6626 1540
Size: 275 186 2605 231 761 1028 2301
Fragments arranged by size:
2605 2301 1028 761 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 3347 3368 5448 5533 6604 3347
Size: 21 2080 85 1071 4130
Fragments arranged by size:
4130 2080 1071 85 21
BsmI GAATG_Cn'
Cuts at: 3419 4898 4991 3419
Size: 1479 93 5815
Fragments arranged by size:
5815 1479 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2299 2398 4041 2299
Size: 99 1643 5645
Fragments arranged by size:
5645 1643 99
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2416 3347 3368 3410 4706 5448 5533
Size: 399 626 931 21 42 1296 742 85
Cuts at: 5533 6604 1391
Size: 1071 2174
Fragments arranged by size:
2174 1296 1071 931 742 626 399 85
42 21
BspEI T'CCGG_A
Cuts at: 2774 7050 2774
Size: 4276 3111
BspHI T'CATG_A
Cuts at: 5276 7006 5276
Size: 1730 5657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4742 844
Size: 3898 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5274 1394
Size: 429 3451 3507
Fragments arranged by size:
3507 3451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5882 6056 65
Size: 1183 4634 174 1396
Fragments arranged by size:
4634 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2903 4580 6031 6218 1700
Size: 110 1093 1677 1451 187 2869
Fragments arranged by size:
2869 1677 1451 1093 187 110
BsrGI T'GTAC_A
Cuts at: 96 2591 96
Size: 2495 4892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7387
BssSI C'ACGA_G
Cuts at: 5447 6459 5447
Size: 1012 6375
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2651 3662 794
Size: 1857 1011 4519
Fragments arranged by size:
4519 1857 1011
BstBI TT'CG_AA
Cuts at: 6191 6191
Size: 7387
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7387
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 7387
BstYI r'GATC_y
Cuts at: 1972 2475 3226 3793 4717 5084 5469 5486 6938
Size: 503 751 567 924 367 385 17 1452
Cuts at: 6938 7024 7036 7382 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 924 751 567 503 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3178 3178
Size: 7387
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5896
Fragments arranged by size:
5896 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2100 487
Size: 683 56 874 5774
Fragments arranged by size:
5774 874 683 56
BtsI GCAGTG_nn'
Cuts at: 3663 4146 4903 5697 5717 3663
Size: 483 757 794 20 5333
Fragments arranged by size:
5333 794 757 483 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2667 2702 2791 2826 4369 4404 529
Size: 35 2103 35 89 35 1543 35 3512
Fragments arranged by size:
3512 2103 1543 89 35 35 35 35
DraI TTT'AAA
Cuts at: 4691 4691
Size: 7387
DraIII CAC_nnn'GTG
Cuts at: 2740 2740
Size: 7387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4405 6394 809
Size: 507 3089 1989 1802
Fragments arranged by size:
3089 1989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 3463 3670
Size: 54 1425 50 275 18 348 1285 207
Cuts at: 3670 4315 4578 4834 5723 7352 8
Size: 645 263 256 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 645 348 275 263
256 207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4834 1537
Size: 275 3022 4090
Fragments arranged by size:
4090 3022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 4611 5317 1353
Size: 1007 2251 706 3423
Fragments arranged by size:
3423 2251 1007 706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5974 6348 6494 387
Size: 1064 138 4385 374 146 1280
Fragments arranged by size:
4385 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3345 3345
Size: 7387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2180 2194 2766 3408 4239 4248
Size: 393 479 69 14 572 642 831 9
Cuts at: 4248 4646 5450 6047 6834 7102 1239
Size: 398 804 597 787 268 1524
Fragments arranged by size:
1524 831 804 787 642 597 572 479
398 393 268 69 14 9
EcoO109I rG'GnC_Cy
Cuts at: 3406 3407 4223 3406
Size: 1 816 6570
Fragments arranged by size:
6570 816 1
EcoRI G'AATT_C
Cuts at: 2214 4696 5074 2214
Size: 2482 378 4527
Fragments arranged by size:
4527 2482 378
EcoRV GAT'ATC
Cuts at: 2584 2584
Size: 7387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2123 3491 2123
Size: 1368 6019
FspI TGC'GCA
Cuts at: 5893 5893
Size: 7387
HaeII r_GCGC'y
Cuts at: 1678 6534 1678
Size: 4856 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2730 2762 3596 3628 3739
Size: 32 135 32 1158 32 834 32 111
Cuts at: 3739 3771 6028 6060 6102 6134 1373
Size: 32 2257 32 42 32 2626
Fragments arranged by size:
2626 2257 1158 834 135 111 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3902 3984 4731 4977 669
Size: 988 281 1964 82 747 246 3079
Fragments arranged by size:
3079 1964 988 747 281 246 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7387
HpaI GTT'AAC
Cuts at: 3984 4977 3984
Size: 993 6394
KpnI G_GTAC'C
Cuts at: 4712 4712
Size: 7387
MfeI C'AATT_G
Cuts at: 4986 4986
Size: 7387
MluI A'CGCG_T
Cuts at: 7263 7263
Size: 7387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4299 4401 6501 6685 1305
Size: 2994 102 2100 184 2007
Fragments arranged by size:
2994 2100 2007 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3465 4317 10
Size: 54 1425 343 1633 852 3080
Fragments arranged by size:
3080 1633 1425 852 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3705 4018 4292 5345 5704 5863 518
Size: 648 2539 313 274 1053 359 159 2042
Fragments arranged by size:
2539 2042 1053 648 359 313 274 159
MspA1I CmG'CkG
Cuts at: 1606 3461 4803 5480 6628 6873 1606
Size: 1855 1342 677 1148 245 2120
Fragments arranged by size:
2120 1855 1342 1148 677 245
NaeI GCC'GGC
Cuts at: 4582 4582
Size: 7387
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5896
Fragments arranged by size:
5896 939 552
NdeI CA'TA_TG
Cuts at: 387 3978 387
Size: 3591 3796
NgoMIV G'CCGG_C
Cuts at: 4580 4580
Size: 7387
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7387
NotI GC'GGCC_GC
Cuts at: 4834 4834
Size: 7387
NsiI A_TGCA'T
Cuts at: 4679 4679
Size: 7387
NspI r_CATG'y
Cuts at: 1132 1229 4137 4297 4677 4745 6290 1132
Size: 97 2908 160 380 68 1545 2229
Fragments arranged by size:
2908 2229 1545 380 160 97 68
PasI CC'CwG_GG
Cuts at: 1348 2027 1348
Size: 679 6708
PciI A'CATG_T
Cuts at: 4133 6286 4133
Size: 2153 5234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7387
PmeI GTTT'AAAC
Cuts at: 4691 4691
Size: 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 6969 7001 5456
Size: 32 1481 32 5842
Fragments arranged by size:
5842 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4223 4223
Size: 7387
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7387
PsiI TTA'TAA
Cuts at: 3921 4957 3921
Size: 1036 6351
PspOMI G'GGCC_C
Cuts at: 3406 3406
Size: 7387
PstI C_TGCA'G
Cuts at: 830 2656 4601 4739 830
Size: 1826 1945 138 3478
Fragments arranged by size:
3478 1945 1826 138
PvuI CG_AT'CG
Cuts at: 2001 4606 2001
Size: 2605 4782
PvuII CAG'CTG
Cuts at: 3461 3461
Size: 7387
SacI G_AGCT'C
Cuts at: 3347 3347
Size: 7387
SalI G'TCGA_C
Cuts at: 1936 4729 1936
Size: 2793 4594
SbfI CC_TGCA'GG
Cuts at: 4739 4739
Size: 7387
ScaI AGT'ACT
Cuts at: 3600 5635 7347 3600
Size: 2035 1712 3640
Fragments arranged by size:
3640 2035 1712
SfcI C'TryA_G
Cuts at: 826 1045 2652 3789 4597 4735 5870 6551 6742
Size: 219 1607 1137 808 138 1135 681 191
Cuts at: 6742 826
Size: 1471
Fragments arranged by size:
1607 1471 1137 1135 808 681 219 191
138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7387
SmaI CCC'GGG
Cuts at: 1872 4714 1872
Size: 2842 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2390 3035 3520 3583 5491
Size: 197 926 228 219 645 485 63 1908
Cuts at: 5491 6392 6654 820
Size: 901 262 1553
Fragments arranged by size:
1908 1553 926 901 645 485 262 228
219 197 63
SnaBI TAC'GTA
Cuts at: 493 5178 493
Size: 4685 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7387
SphI G_CATG'C
Cuts at: 1132 4745 1132
Size: 3613 3774
SspI AAT'ATT
Cuts at: 5 52 3643 4252 5311 5
Size: 47 3591 609 1059 2081
Fragments arranged by size:
3591 2081 1059 609 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7387
StyI C'CwwG_G
Cuts at: 513 1065 2004 3475 4401 4826 513
Size: 552 939 1471 926 425 3074
Fragments arranged by size:
3074 1471 939 926 552 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2910 2975 4487 5414 5431 5584 5769
Size: 1255 1330 65 1512 927 17 153 185
Cuts at: 5769 6214 325
Size: 445 1498
Fragments arranged by size:
1512 1498 1330 1255 927 445 185 153
65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 2591 3598
Size: 274 80 33 51 1443 355 259 1007
Cuts at: 3598 4040 5633 7345 96
Size: 442 1593 1712 138
Fragments arranged by size:
1712 1593 1443 1007 442 355 274 259
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 7347 7379 2769
Size: 32 4546 32 2777
Fragments arranged by size:
4546 2777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7387
XbaI T'CTAG_A
Cuts at: 2218 4633 4723 2218
Size: 2415 90 4882
Fragments arranged by size:
4882 2415 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3473 4481 3473
Size: 1008 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7387
XmaI C'CCGG_G
Cuts at: 1870 4712 1870
Size: 2842 4545
XmnI GAAnn'nnTTC
Cuts at: 5516 5516
Size: 7387
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5195 276
Size: 53 83 186 936 3661 2468
Fragments arranged by size:
3661 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI Bsu36I EcoNI FseI FspAI KasI NarI
NruI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI