Restriction map for
pFN21AB7340
(Circular) (Six-base) MAPSORT of: pFN21AB7340.seq Check: 4447 from: 1 to: 8173
pFN21AB7340 8173 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3073 3073
Size: 8173
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5983 278
Size: 53 83 186 936 4447 2468
Fragments arranged by size:
4447 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2640 3717 3861 4517 5516 1937
Size: 703 1077 144 656 999 4594
Fragments arranged by size:
4594 1077 999 703 656 144
Acc65I G'GTAC_C
Cuts at: 5494 5494
Size: 8173
AclI AA'CG_TT
Cuts at: 1563 6300 6673 1563
Size: 4737 373 3063
Fragments arranged by size:
4737 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 2590 3744 4782 6236 7620 7888 1632
Size: 534 424 1154 1038 1454 1384 268 1917
Fragments arranged by size:
1917 1454 1384 1154 1038 534 424 268
AfeI AGC'GCT
Cuts at: 2947 2947
Size: 8173
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7976
AflIII A'CryG_T
Cuts at: 1203 5452 7072 8049 1203
Size: 4249 1620 977 1327
Fragments arranged by size:
4249 1620 1327 977
AgeI A'CCGG_T
Cuts at: 7004 7004
Size: 8173
AhdI GACnn_n'nnGTC
Cuts at: 6902 6902
Size: 8173
AleI CACnn'nnGTG
Cuts at: 1166 4046 4421 1166
Size: 2880 375 4918
Fragments arranged by size:
4918 2880 375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8173
AlwNI CAG_nnn'CTG
Cuts at: 2979 3996 4810 5916 7488 2979
Size: 1017 814 1106 1572 3664
Fragments arranged by size:
3664 1572 1106 1017 814
ApaI G_GGCC'C
Cuts at: 2464 2475 4333 2464
Size: 11 1858 6304
Fragments arranged by size:
6304 1858 11
ApaLI G'TGCA_C
Cuts at: 6230 7386 6230
Size: 1156 7017
ApoI r'AATT_y
Cuts at: 1493 2024 3649 3808 3947 4322 5293 5399 5482
Size: 531 1625 159 139 375 971 106 83
Cuts at: 5482 5708 5860 7969 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 971 531 375 226 159
152 139 106 83
AseI AT'TA_AT
Cuts at: 160 1032 6727 160
Size: 872 5695 1606
Fragments arranged by size:
5695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8173
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2091 2229 2320 3844 5498 1870
Size: 73 90 58 138 91 1524 1654 4545
Fragments arranged by size:
4545 1654 1524 138 91 90 73 58
AvrII C'CTAG_G
Cuts at: 2872 2872
Size: 8173
BamHI G'GATC_C
Cuts at: 2349 2497 4685 5503 5870 2349
Size: 148 2188 818 367 4652
Fragments arranged by size:
4652 2188 818 367 148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2072 2582 3840 4267
Size: 325 217 627 250 35 510 1258 427
Cuts at: 4267 5321 5494 6949 618
Size: 1054 173 1455 1842
Fragments arranged by size:
1842 1455 1258 1054 627 510 427 325
250 217 173 35
BanII G_rGCy'C
Cuts at: 1391 2119 2464 2475 2699 3122 3800 4333 5492
Size: 728 345 11 224 423 678 533 1159
Cuts at: 5492 1391
Size: 4072
Fragments arranged by size:
4072 1159 728 678 533 423 345 224
11
BbeI G_GCGC'C
Cuts at: 2076 2076
Size: 8173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2297 928
Size: 971 398 6804
Fragments arranged by size:
6804 971 398
BbvCI CC'TCA_GC
Cuts at: 3939 4606 3939
Size: 667 7506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2182 2216 2986 3020 6364 6398 1903
Size: 34 245 34 770 34 3344 34 3678
Fragments arranged by size:
3678 3344 770 245 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3733 6065 7275 3733
Size: 2332 1210 4631
Fragments arranged by size:
4631 2332 1210
BclI T'GATC_A
Cuts at: 1548 3427 1548
Size: 1879 6294
BfrBI ATG'CAT
Cuts at: 4105 4105
Size: 8173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6784 136
Size: 107 122 71 631 605 5112 1525
Fragments arranged by size:
5112 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8168 8168
Size: 8173
BlpI GC'TnA_GC
Cuts at: 3069 4887 5590 3069
Size: 1818 703 5652
Fragments arranged by size:
5652 1818 703
Bme1580I G_kGCm'C
Cuts at: 1790 2464 2475 2585 2752 4270 4333 4372 4874
Size: 674 11 110 167 1518 63 39 502
Cuts at: 4874 6234 7390 1790
Size: 1360 1156 2573
Fragments arranged by size:
2573 1518 1360 1156 674 502 167 110
63 39 11
BmgBI CAC'GTC
Cuts at: 1318 2596 3657 1318
Size: 1278 1061 5834
Fragments arranged by size:
5834 1278 1061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2331 3984 4359
Size: 416 53 83 399 24 914 1653 375
Cuts at: 4359 4971 5085 5904 6862 442
Size: 612 114 819 958 1753
Fragments arranged by size:
1753 1653 958 914 819 612 416 399
375 114 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5226 1056
Size: 4170 4003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2967 2967
Size: 8173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2648 2673 3772 4014 4389 4513 4998
Size: 1223 186 25 1099 242 375 124 485
Cuts at: 4998 6833 1239
Size: 1835 2579
Fragments arranged by size:
2579 1835 1223 1099 485 375 242 186
124 25
Bpu10I CC'TnA_GC
Cuts at: 2543 2793 3782 3939 4007 4382 4606 2543
Size: 250 989 157 68 375 224 6110
Fragments arranged by size:
6110 989 375 250 224 157 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6298 7163 7461 6298
Size: 865 298 7010
Fragments arranged by size:
7010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2441 3090 3158 3268 4642 6836 882
Size: 617 942 649 68 110 1374 2194 2219
Fragments arranged by size:
2219 2194 1374 942 649 617 110 68
BsaAI yAC'GTr
Cuts at: 493 1204 2396 5964 493
Size: 711 1192 3568 2702
Fragments arranged by size:
3568 2702 1192 711
BsaBI GATnn'nnATC
Cuts at: 2496 8167 2496
Size: 5671 2502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2097 2684 5980
Size: 53 83 186 936 540 24 587 3296
Cuts at: 5980 6362 6997 275
Size: 382 635 1451
Fragments arranged by size:
3296 1451 936 635 587 540 382 186
83 53 24
BsaWI w'CCGG_w
Cuts at: 2500 2773 2897 5470 6606 7004 7278 7425 7836
Size: 273 124 2573 1136 398 274 147 411
Cuts at: 7836 2500
Size: 2837
Fragments arranged by size:
2837 2573 1136 411 398 274 273 147
124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 4099 4129 4474
Size: 30 165 30 1182 30 1484 30 345
Cuts at: 4474 4504 1178
Size: 30 4847
Fragments arranged by size:
4847 1484 1182 345 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 2459 2943 3378 3567 3570 4017 4104
Size: 1160 113 484 435 189 3 447 87
Cuts at: 4104 4553 1186
Size: 449 4806
Fragments arranged by size:
4806 1160 484 449 447 435 189 113
87 3
BseYI C'CCAG_C
Cuts at: 2360 2428 2723 2864 3189 4208 4768 5141 7376
Size: 68 295 141 325 1019 560 373 2235
Cuts at: 7376 7872 2360
Size: 496 2661
Fragments arranged by size:
2661 2235 1019 560 496 373 325 295
141 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 3239 3849 4236 4617 5193 1163
Size: 573 789 714 610 387 381 576 4143
Fragments arranged by size:
4143 789 714 610 576 573 387 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2113 2241 5623 6384 7412 1540
Size: 275 186 112 128 3382 761 1028 2301
Fragments arranged by size:
3382 2301 1028 761 275 186 128 112
BsiHKAI G_wGCw'C
Cuts at: 2699 3800 6234 6319 7390 2699
Size: 1101 2434 85 1071 3482
Fragments arranged by size:
3482 2434 1101 1071 85
BsmI GAATG_Cn'
Cuts at: 3368 5684 5777 3368
Size: 2316 93 5764
Fragments arranged by size:
5764 2316 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2586 2691 2586
Size: 105 8068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2464 2475 2585 2699 2752 3122
Size: 399 329 345 11 110 114 53 370
Cuts at: 3122 3800 4270 4333 4372 4874 5492 6234 6319
Size: 678 470 63 39 502 618 742 85
Cuts at: 6319 7390 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 678 618 502 470 399
370 345 329 114 110 85 63 53
39 11
BspEI T'CCGG_A
Cuts at: 2773 2897 7836 2773
Size: 124 4939 3110
Fragments arranged by size:
4939 3110 124
BspHI T'CATG_A
Cuts at: 6062 7792 6062
Size: 1730 6443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3073 5528 844
Size: 2229 2455 3489
Fragments arranged by size:
3489 2455 2229
BsrBI CCG'CTC
Cuts at: 1394 1823 2081 6060 1394
Size: 429 258 3979 3507
Fragments arranged by size:
3979 3507 429 258
BsrDI GCAATG_nn'
Cuts at: 65 1248 3002 3032 3820 4599 6668 6842 65
Size: 1183 1754 30 788 779 2069 174 1396
Fragments arranged by size:
2069 1754 1396 1183 788 779 174 30
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2260 2401 6817 7004 1700
Size: 110 236 214 141 4416 187 2869
Fragments arranged by size:
4416 2869 236 214 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8173
BssSI C'ACGA_G
Cuts at: 6233 7245 6233
Size: 1012 7161
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8173
BstBI TT'CG_AA
Cuts at: 6977 6977
Size: 8173
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8173
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 3039 3920 5426 2440
Size: 599 881 1506 5187
Fragments arranged by size:
5187 1506 881 599
BstYI r'GATC_y
Cuts at: 1972 2349 2497 4685 5327 5503 5870 6255 6272
Size: 377 148 2188 642 176 367 385 17
Cuts at: 6272 7724 7810 7822 8168 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2188 1977 1452 642 385 377 367 346
176 148 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2146 513
Size: 552 939 142 6540
Fragments arranged by size:
6540 939 552 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7434
Fragments arranged by size:
7434 683 56
BtsI GCAGTG_nn'
Cuts at: 3026 3832 4218 4435 5308 5689 6483 6503 3026
Size: 806 386 217 873 381 794 20 4696
Fragments arranged by size:
4696 873 806 794 386 381 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8138
DraI TTT'AAA
Cuts at: 4296 4677 5477 4296
Size: 381 800 6992
Fragments arranged by size:
6992 800 381
DraIII CAC_nnn'GTG
Cuts at: 2396 4814 2396
Size: 2418 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7180 809
Size: 507 5864 1802
Fragments arranged by size:
5864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2280 5191
Size: 54 1425 50 275 18 408 42 2911
Cuts at: 5191 5620 6509 8138 8
Size: 429 889 1629 43
Fragments arranged by size:
2911 1629 1425 889 429 408 275 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2238 5620 1537
Size: 275 426 3382 4090
Fragments arranged by size:
4090 3382 426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2473 2847 3110 3402 3603 6103 1353
Size: 1120 374 263 292 201 2500 3423
Fragments arranged by size:
3423 2500 1120 374 292 263 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 6760 7134 7280 387
Size: 1064 138 558 4613 374 146 1280
Fragments arranged by size:
4613 1280 1064 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 2697 3798 2697
Size: 1101 7072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2462 2590 2648 2673 3744 3772
Size: 393 534 296 128 58 25 1071 28
Cuts at: 3772 4014 4389 4513 4782 4998 6236 6833 7620
Size: 242 375 124 269 216 1238 597 787
Cuts at: 7620 7888 1239
Size: 268 1524
Fragments arranged by size:
1524 1238 1071 787 597 534 393 375
296 269 268 242 216 128 124 58
28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2983 2983
Size: 8173
EcoO109I rG'GnC_Cy
Cuts at: 2254 2471 2535 2574 2711 2976 3621 4329 4330
Size: 217 64 39 137 265 645 708 1
Cuts at: 4330 5033 5087 5182 5342 2254
Size: 703 54 95 160 5085
Fragments arranged by size:
5085 708 703 645 265 217 160 137
95 64 54 39 1
EcoRI G'AATT_C
Cuts at: 3649 5293 5482 5860 3649
Size: 1644 189 378 5962
Fragments arranged by size:
5962 1644 378 189
FspI TGC'GCA
Cuts at: 6679 6679
Size: 8173
HaeII r_GCGC'y
Cuts at: 1678 2076 2789 2949 3479 7320 1678
Size: 398 713 160 530 3841 2531
Fragments arranged by size:
3841 2531 713 530 398 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 2341 2373 2585
Size: 32 135 32 631 32 106 32 212
Cuts at: 2585 2617 3300 3332 4835 4867 6814 6846 6888
Size: 32 683 32 1503 32 1947 32 42
Cuts at: 6888 6920 1373
Size: 32 2626
Fragments arranged by size:
2626 1947 1503 683 631 212 135 106
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4848 5517 5763 669
Size: 988 281 2910 669 246 3079
Fragments arranged by size:
3079 2910 988 669 281 246
HindIII A'AGCT_T
Cuts at: 748 3978 748
Size: 3230 4943
HpaI GTT'AAC
Cuts at: 4848 5763 4848
Size: 915 7258
KasI G'GCGC_C
Cuts at: 2072 2072
Size: 8173
KpnI G_GTAC'C
Cuts at: 5498 5498
Size: 8173
MfeI C'AATT_G
Cuts at: 5772 5772
Size: 8173
MluI A'CGCG_T
Cuts at: 8049 8049
Size: 8173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4308 4978 5317 5488 7287 7471 1305
Size: 3003 670 339 171 1799 184 2007
Fragments arranged by size:
3003 2007 1799 670 339 184 171
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 5193 10
Size: 54 1425 343 450 2911 2990
Fragments arranged by size:
2990 2911 1425 450 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3037 3825 4046 4421 4964 5424 6131
Size: 648 1871 788 221 375 543 460 707
Cuts at: 6131 6490 6649 518
Size: 359 159 2042
Fragments arranged by size:
2042 1871 788 707 648 543 460 375
359 221 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2550 2890 3016 4212 5589 6266 7414
Size: 542 402 340 126 1196 1377 677 1148
Cuts at: 7414 7659 1606
Size: 245 2120
Fragments arranged by size:
2120 1377 1196 1148 677 542 402 340
245 126
NaeI GCC'GGC
Cuts at: 2048 2262 2403 2048
Size: 214 141 7818
Fragments arranged by size:
7818 214 141
NarI GG'CG_CC
Cuts at: 2073 2073
Size: 8173
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6682
Fragments arranged by size:
6682 939 552
NdeI CA'TA_TG
Cuts at: 387 5440 387
Size: 5053 3120
NgoMIV G'CCGG_C
Cuts at: 2046 2260 2401 2046
Size: 214 141 7818
Fragments arranged by size:
7818 214 141
NheI G'CTAG_C
Cuts at: 1052 5222 1052
Size: 4170 4003
NotI GC'GGCC_GC
Cuts at: 2238 5620 2238
Size: 3382 4791
NsiI A_TGCA'T
Cuts at: 4107 4107
Size: 8173
NspI r_CATG'y
Cuts at: 1132 1229 2371 5077 5456 5531 7076 1132
Size: 97 1142 2706 379 75 1545 2229
Fragments arranged by size:
2706 2229 1545 1142 379 97 75
PasI CC'CwG_GG
Cuts at: 1348 2578 2587 2618 1348
Size: 1230 9 31 6903
Fragments arranged by size:
6903 1230 31 9
PciI A'CATG_T
Cuts at: 5452 7072 5452
Size: 1620 6553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2069 2804 4814 5200 1108
Size: 961 735 2010 386 4081
Fragments arranged by size:
4081 2010 961 735 386
PfoI T'CCnGG_A
Cuts at: 2439 4300 4529 2439
Size: 1861 229 6083
Fragments arranged by size:
6083 1861 229
PmeI GTTT'AAAC
Cuts at: 5477 5477
Size: 8173
PmlI CAC'GTG
Cuts at: 2396 2396
Size: 8173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 6242 6274 7755 7787 4043
Size: 32 2167 32 1481 32 4429
Fragments arranged by size:
4429 2167 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2535 2574 3621 5033 5182 2254
Size: 281 39 1047 1412 149 5245
Fragments arranged by size:
5245 1412 1047 281 149 39
PshAI GACnn'nnGTC
Cuts at: 1613 3857 1613
Size: 2244 5929
PsiI TTA'TAA
Cuts at: 3456 5743 3456
Size: 2287 5886
PspOMI G'GGCC_C
Cuts at: 2460 2471 4329 2460
Size: 11 1858 6304
Fragments arranged by size:
6304 1858 11
PstI C_TGCA'G
Cuts at: 830 2823 4441 5379 5525 830
Size: 1993 1618 938 146 3478
Fragments arranged by size:
3478 1993 1618 938 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8173
SacI G_AGCT'C
Cuts at: 2699 3800 2699
Size: 1101 7072
SacII CC_GC'GG
Cuts at: 2149 2149
Size: 8173
SalI G'TCGA_C
Cuts at: 1936 5515 1936
Size: 3579 4594
SanDI GG'GwC_CC
Cuts at: 5182 5182
Size: 8173
SapI GCTCTTCn'nnn_
Cuts at: 2847 3110 3402 2847
Size: 263 292 7618
Fragments arranged by size:
7618 292 263
SbfI CC_TGCA'GG
Cuts at: 5525 5525
Size: 8173
ScaI AGT'ACT
Cuts at: 6421 8133 6421
Size: 1712 6461
SexAI A'CCwGG_T
Cuts at: 4831 4831
Size: 8173
SfcI C'TryA_G
Cuts at: 826 1045 2819 4062 4275 4437 5375 5521 6656
Size: 219 1774 1243 213 162 938 146 1135
Cuts at: 6656 7337 7528 826
Size: 681 191 1471
Fragments arranged by size:
1774 1471 1243 1135 938 681 219 213
191 162 146
SfoI GGC'GCC
Cuts at: 2074 2074
Size: 8173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8173
SmaI CCC'GGG
Cuts at: 1872 2035 2093 3846 5500 1872
Size: 163 58 1753 1654 4545
Fragments arranged by size:
4545 1753 1654 163 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6277 7178 7440 820
Size: 197 926 4334 901 262 1553
Fragments arranged by size:
4334 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5964 493
Size: 5471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8173
SphI G_CATG'C
Cuts at: 1132 5531 1132
Size: 4399 3774
SspI AAT'ATT
Cuts at: 5 52 6097 5
Size: 47 6045 2081
Fragments arranged by size:
6045 2081 47
StuI AGG'CCT
Cuts at: 1517 2179 1517
Size: 662 7511
StyI C'CwwG_G
Cuts at: 513 1065 2004 2872 2939 3108 4122 4231 5612
Size: 552 939 868 67 169 1014 109 1381
Cuts at: 5612 513
Size: 3074
Fragments arranged by size:
3074 1381 1014 939 868 552 169 109
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 2465 2600 3203 3413 5155 5432
Size: 1255 731 154 135 603 210 1742 277
Cuts at: 5432 6200 6217 6370 6555 7000 325
Size: 768 17 153 185 445 1498
Fragments arranged by size:
1742 1498 1255 768 731 603 445 277
210 185 154 153 135 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4542 6419 8131
Size: 274 80 33 51 1443 2565 1877 1712
Cuts at: 8131 96
Size: 138
Fragments arranged by size:
2565 1877 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 4612 4644 8133 8165 2324
Size: 32 2256 32 3489 32 2332
Fragments arranged by size:
3489 2332 2256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8173
XbaI T'CTAG_A
Cuts at: 5509 5509
Size: 8173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3286 3349 4811 3286
Size: 63 1462 6648
Fragments arranged by size:
6648 1462 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8173
XmaI C'CCGG_G
Cuts at: 1870 2033 2091 3844 5498 1870
Size: 163 58 1753 1654 4545
Fragments arranged by size:
4545 1753 1654 163 58
XmnI GAAnn'nnTTC
Cuts at: 2924 6302 2924
Size: 3378 4795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5981 276
Size: 53 83 186 936 4447 2468
Fragments arranged by size:
4447 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI BstZ17I Bsu36I EcoRV FalI
FseI FspAI NruI PacI PspXI PsrI PvuII RsrII
SfiI SrfI SwaI