Restriction map for
pFN21AB7335
(Circular) (Six-base) MAPSORT of: pFN21AB7335.seq Check: 8522 from: 1 to: 7765
pFN21AB7335 7765 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2038 4964 2038
Size: 2926 4839
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5575 278
Size: 53 83 186 936 4039 2468
Fragments arranged by size:
4039 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3024 5108 1937
Size: 1087 2084 4594
Fragments arranged by size:
4594 2084 1087
Acc65I G'GTAC_C
Cuts at: 2248 5086 2248
Size: 2838 4927
AclI AA'CG_TT
Cuts at: 1563 5892 6265 1563
Size: 4329 373 3063
Fragments arranged by size:
4329 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3518 4494 4509 4602 5828 7212 7480
Size: 512 1374 976 15 93 1226 1384 268
Cuts at: 7480 1632
Size: 1917
Fragments arranged by size:
1917 1384 1374 1226 976 512 268 93
15
AfeI AGC'GCT
Cuts at: 4394 4394
Size: 7765
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7568
AflIII A'CryG_T
Cuts at: 1203 6664 7641 1203
Size: 5461 977 1327
Fragments arranged by size:
5461 1327 977
AgeI A'CCGG_T
Cuts at: 6596 6596
Size: 7765
AhdI GACnn_n'nnGTC
Cuts at: 6494 6494
Size: 7765
AleI CACnn'nnGTG
Cuts at: 1166 3629 4596 4724 1166
Size: 2463 967 128 4207
Fragments arranged by size:
4207 2463 967 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7765
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 2211
Size: 32 7733
AlwNI CAG_nnn'CTG
Cuts at: 2506 3554 4201 4792 5508 7080 2506
Size: 1048 647 591 716 1572 3191
Fragments arranged by size:
3191 1572 1048 716 647 591
ApaI G_GGCC'C
Cuts at: 3365 3365
Size: 7765
ApaLI G'TGCA_C
Cuts at: 3429 5822 6978 3429
Size: 2393 1156 4216
Fragments arranged by size:
4216 2393 1156
ApoI r'AATT_y
Cuts at: 1493 3715 5074 5300 5452 7561 1493
Size: 2222 1359 226 152 2109 1697
Fragments arranged by size:
2222 2109 1697 1359 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2939 6319 160
Size: 872 1907 3380 1606
Fragments arranged by size:
3380 1907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7765
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 3366 3799 5090 1870
Size: 73 316 1107 433 1291 4545
Fragments arranged by size:
4545 1291 1107 433 316 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2793 2826 4865 4898 2793
Size: 33 2039 33 5660
Fragments arranged by size:
5660 2039 33 33
BamHI G'GATC_C
Cuts at: 5095 5462 5095
Size: 367 7398
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2248 3187 3337 4288 5086
Size: 325 217 627 461 939 150 951 798
Cuts at: 5086 6541 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 951 939 798 627 461 325
217 150
BanII G_rGCy'C
Cuts at: 1391 3365 4710 5084 1391
Size: 1974 1345 374 4072
Fragments arranged by size:
4072 1974 1345 374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2646 928
Size: 971 747 6047
Fragments arranged by size:
6047 971 747
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3428 3462 3725 3759 5956 5990 1903
Size: 34 1491 34 263 34 2197 34 3678
Fragments arranged by size:
3678 2197 1491 263 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5657 6867 5657
Size: 1210 6555
BclI T'GATC_A
Cuts at: 1548 2787 1548
Size: 1239 6526
BfrBI ATG'CAT
Cuts at: 3742 3987 3742
Size: 245 7520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6376 136
Size: 107 122 71 631 605 4704 1525
Fragments arranged by size:
4704 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2349 2448 7760 2349
Size: 99 5312 2354
Fragments arranged by size:
5312 2354 99
BlpI GC'TnA_GC
Cuts at: 4345 4856 5182 4345
Size: 511 326 6928
Fragments arranged by size:
6928 511 326
Bme1580I G_kGCm'C
Cuts at: 1790 2928 3365 3433 3931 4291 5826 6982 1790
Size: 1138 437 68 498 360 1535 1156 2573
Fragments arranged by size:
2573 1535 1156 1138 498 437 360 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2678 3642 3667
Size: 416 53 83 399 24 1261 964 25
Cuts at: 3667 5496 6454 442
Size: 1829 958 1753
Fragments arranged by size:
1829 1753 1261 964 958 416 399 83
53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7765
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 4717 3614
Size: 1103 6662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3371 3597 3626 3782 3895 3907 4559 4712
Size: 2132 226 29 156 113 12 652 153
Cuts at: 4712 4769 5003 6425 1239
Size: 57 234 1422 2579
Fragments arranged by size:
2579 2132 1422 652 234 226 156 153
113 57 29 12
Bpu10I CC'TnA_GC
Cuts at: 2309 2389 2991 3555 2309
Size: 80 602 564 6519
Fragments arranged by size:
6519 602 564 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 5890 6755 7053 4075
Size: 1815 865 298 4787
Fragments arranged by size:
4787 1815 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3133 4930 6428 882
Size: 617 1634 1797 1498 2219
Fragments arranged by size:
2219 1797 1634 1498 617
BsaAI yAC'GTr
Cuts at: 493 1204 5556 493
Size: 711 4352 2702
Fragments arranged by size:
4352 2702 711
BsaBI GATnn'nnATC
Cuts at: 7759 7759
Size: 7765
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2685 4364 5572 5954
Size: 53 83 186 936 1152 1679 1208 382
Cuts at: 5954 6589 275
Size: 635 1451
Fragments arranged by size:
1679 1451 1208 1152 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6198 6596 6870 7017 7428 6198
Size: 398 274 147 411 6535
Fragments arranged by size:
6535 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 4619 4649 4727
Size: 30 165 30 936 30 2250 30 78
Cuts at: 4727 4757 1178
Size: 30 4186
Fragments arranged by size:
4186 2250 936 165 78 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2464 2904 3032 3535 3838 4448 4962 1186
Size: 1278 440 128 503 303 610 514 3989
Fragments arranged by size:
3989 1278 610 514 503 440 303 128
BseYI C'CCAG_C
Cuts at: 2042 2535 4183 4452 6968 7464 2042
Size: 493 1648 269 2516 496 2343
Fragments arranged by size:
2516 2343 1648 496 493 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 2153 2793 3719 3853 1163
Size: 573 369 48 640 926 134 5075
Fragments arranged by size:
5075 926 640 573 369 134 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5215 5976 7004 1540
Size: 275 186 3214 761 1028 2301
Fragments arranged by size:
3214 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3123 3433 3636 4710 5826 5911 6982 3123
Size: 310 203 1074 1116 85 1071 3906
Fragments arranged by size:
3906 1116 1074 1071 310 203 85
BsiWI C'GTAC_G
Cuts at: 3217 3217
Size: 7765
BsmI GAATG_Cn'
Cuts at: 5276 5369 5276
Size: 93 7672
BsmBI CGTCTCn'nnnn_
Cuts at: 2692 2692
Size: 7765
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2928 3123 3365 3433 3636 3931 4291
Size: 399 1138 195 242 68 203 295 360
Cuts at: 4291 4710 5084 5826 5911 6982 1391
Size: 419 374 742 85 1071 2174
Fragments arranged by size:
2174 1138 1071 742 419 399 374 360
295 242 203 195 85 68
BspEI T'CCGG_A
Cuts at: 7428 7428
Size: 7765
BspHI T'CATG_A
Cuts at: 5654 7384 5654
Size: 1730 6035
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 4964 5120 844
Size: 1194 2926 156 3489
Fragments arranged by size:
3489 2926 1194 156
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 4344 5652 1394
Size: 429 1765 756 1308 3507
Fragments arranged by size:
3507 1765 1308 756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2961 3814 4446 6260 6434 65
Size: 1183 1713 853 632 1814 174 1396
Fragments arranged by size:
1814 1713 1396 1183 853 632 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4027 4209 6409 6596 1700
Size: 110 2217 182 2200 187 2869
Fragments arranged by size:
2869 2217 2200 187 182 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7765
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7765
BssSI C'ACGA_G
Cuts at: 5825 6837 5825
Size: 1012 6753
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7765
BstBI TT'CG_AA
Cuts at: 6569 6569
Size: 7765
BstEII G'GTnAC_C
Cuts at: 1184 2263 2800 1184
Size: 1079 537 6149
Fragments arranged by size:
6149 1079 537
BstXI CCAn_nnnn'nTGG
Cuts at: 3650 3650
Size: 7765
BstYI r'GATC_y
Cuts at: 1972 2349 2423 2448 3837 4065 4806 4968 5095
Size: 377 74 25 1389 228 741 162 127
Cuts at: 5095 5462 5847 5864 7316 7402 7414 7760 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1389 741 385 377 367 346
228 162 127 86 74 25 17 12
Bsu36I CC'TnA_GG
Cuts at: 3357 3357
Size: 7765
BtgI C'CryG_G
Cuts at: 513 1065 2004 2691 3705 4826 513
Size: 552 939 687 1014 1121 3452
Fragments arranged by size:
3452 1121 1014 939 687 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2935 3728 3742 487
Size: 683 56 1709 793 14 4510
Fragments arranged by size:
4510 1709 793 683 56 14
BtsI GCAGTG_nn'
Cuts at: 2744 2834 2860 3067 4322 4516 5281 6075 6095
Size: 90 26 207 1255 194 765 794 20
Cuts at: 6095 2744
Size: 4414
Fragments arranged by size:
4414 1255 794 765 207 194 90 26
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7765
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4311 4346 529
Size: 35 3747 35 3948
Fragments arranged by size:
3948 3747 35 35
DraI TTT'AAA
Cuts at: 5069 5069
Size: 7765
DraIII CAC_nnn'GTG
Cuts at: 2503 2503
Size: 7765
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4958 6772 809
Size: 507 3642 1814 1802
Fragments arranged by size:
3642 1814 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 2917 3258
Size: 54 1425 50 275 18 765 322 341
Cuts at: 3258 5212 6101 7730 8
Size: 1954 889 1629 43
Fragments arranged by size:
1954 1629 1425 889 765 341 322 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5212 1537
Size: 275 3400 4090
Fragments arranged by size:
4090 3400 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3605 4056 4357 4578 4622 5695 1353
Size: 2252 451 301 221 44 1073 3423
Fragments arranged by size:
3423 2252 1073 451 301 221 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6352 6726 6872 387
Size: 1064 138 4763 374 146 1280
Fragments arranged by size:
4763 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4708 4708
Size: 7765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 3371 3518 3597 3626 3782 3895
Size: 393 512 1227 147 79 29 156 113
Cuts at: 3895 3907 4494 4509 4559 4602 4712 4769 5003
Size: 12 587 15 50 43 110 57 234
Cuts at: 5003 5828 6425 7212 7480 1239
Size: 825 597 787 268 1524
Fragments arranged by size:
1524 1227 825 787 597 587 512 393
268 234 156 147 113 110 79 57
50 43 29 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 5056 2582
Size: 2474 5291
EcoO109I rG'GnC_Cy
Cuts at: 2662 2970 3270 3361 3362 3979 4198 4687 4757
Size: 308 300 91 1 617 219 489 70
Cuts at: 4757 4789 4901 2662
Size: 32 112 5526
Fragments arranged by size:
5526 617 489 308 300 219 112 91
70 32 1
EcoRI G'AATT_C
Cuts at: 5074 5452 5074
Size: 378 7387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4291 4499 4291
Size: 208 7557
FspI TGC'GCA
Cuts at: 3059 3317 6271 3059
Size: 258 2954 4553
Fragments arranged by size:
4553 2954 258
FspAI rTGC'GCAy
Cuts at: 3317 3317
Size: 7765
HaeII r_GCGC'y
Cuts at: 1678 2020 3817 4396 6912 1678
Size: 342 1797 579 2516 2531
Fragments arranged by size:
2531 2516 1797 579 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 2689 2721 4637
Size: 32 135 32 843 32 242 32 1916
Cuts at: 4637 4669 6406 6438 6480 6512 1373
Size: 32 1737 32 42 32 2626
Fragments arranged by size:
2626 1916 1737 843 242 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5109 5355 669
Size: 988 281 3171 246 3079
Fragments arranged by size:
3171 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7765
HpaI GTT'AAC
Cuts at: 5355 5355
Size: 7765
KpnI G_GTAC'C
Cuts at: 2252 5090 2252
Size: 2838 4927
MfeI C'AATT_G
Cuts at: 3275 5364 3275
Size: 2089 5676
MluI A'CGCG_T
Cuts at: 7641 7641
Size: 7765
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2283 4504 6879 7063 1305
Size: 978 2221 2375 184 2007
Fragments arranged by size:
2375 2221 2007 978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 2919 3260 10
Size: 54 1425 343 765 322 341 4515
Fragments arranged by size:
4515 1425 765 343 341 322 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2954 3629 4529 4596 4724 5723 6082
Size: 648 1788 675 900 67 128 999 359
Cuts at: 6082 6241 518
Size: 159 2042
Fragments arranged by size:
2042 1788 999 900 675 648 359 159
128 67
MspA1I CmG'CkG
Cuts at: 1606 2385 2539 2702 4580 4879 5181 5858 7006
Size: 779 154 163 1878 299 302 677 1148
Cuts at: 7006 7251 1606
Size: 245 2120
Fragments arranged by size:
2120 1878 1148 779 677 302 299 245
163 154
NcoI C'CATG_G
Cuts at: 513 1065 2004 2691 3705 513
Size: 552 939 687 1014 4573
Fragments arranged by size:
4573 1014 939 687 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7765
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7765
NotI GC'GGCC_GC
Cuts at: 5212 5212
Size: 7765
NsiI A_TGCA'T
Cuts at: 3744 3989 3744
Size: 245 7520
NspI r_CATG'y
Cuts at: 1132 1229 3316 3742 5123 6668 1132
Size: 97 2087 426 1381 1545 2229
Fragments arranged by size:
2229 2087 1545 1381 426 97
PasI CC'CwG_GG
Cuts at: 1348 2201 1348
Size: 853 6912
PciI A'CATG_T
Cuts at: 6664 6664
Size: 7765
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2321 2506 4745 1108
Size: 1213 185 2239 4128
Fragments arranged by size:
4128 2239 1213 185
PfoI T'CCnGG_A
Cuts at: 3759 4785 3759
Size: 1026 6739
PmeI GTTT'AAAC
Cuts at: 5069 5069
Size: 7765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5834 5866 7347 7379 5834
Size: 32 1481 32 6220
Fragments arranged by size:
6220 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2662 2970 3270 4757 4789 4901 2662
Size: 308 300 1487 32 112 5526
Fragments arranged by size:
5526 1487 308 300 112 32
PshAI GACnn'nnGTC
Cuts at: 1613 2491 1613
Size: 878 6887
PsiI TTA'TAA
Cuts at: 5335 5335
Size: 7765
PspOMI G'GGCC_C
Cuts at: 3361 3361
Size: 7765
PspXI vC'TCGA_Gb
Cuts at: 3366 3366
Size: 7765
PstI C_TGCA'G
Cuts at: 830 2493 2556 2610 2751 2912 3498 4511 4579
Size: 1663 63 54 141 161 586 1013 68
Cuts at: 4579 5117 830
Size: 538 3478
Fragments arranged by size:
3478 1663 1013 586 538 161 141 68
63 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7765
PvuII CAG'CTG
Cuts at: 2702 4580 4879 2702
Size: 1878 299 5588
Fragments arranged by size:
5588 1878 299
SacI G_AGCT'C
Cuts at: 4710 4710
Size: 7765
SalI G'TCGA_C
Cuts at: 1936 5107 1936
Size: 3171 4594
SanDI GG'GwC_CC
Cuts at: 3270 3270
Size: 7765
SbfI CC_TGCA'GG
Cuts at: 5117 5117
Size: 7765
ScaI AGT'ACT
Cuts at: 6013 7725 6013
Size: 1712 6053
SexAI A'CCwGG_T
Cuts at: 2499 3123 4315 2499
Size: 624 1192 5949
Fragments arranged by size:
5949 1192 624
SfcI C'TryA_G
Cuts at: 826 1045 2489 2552 2606 2747 2908 3235 3494
Size: 219 1444 63 54 141 161 327 259
Cuts at: 3494 3817 4507 4575 5113 6248 6929 7120 826
Size: 323 690 68 538 1135 681 191 1471
Fragments arranged by size:
1471 1444 1135 690 681 538 327 323
259 219 191 161 141 68 63 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7765
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7765
SmaI CCC'GGG
Cuts at: 1872 2261 3801 5092 1872
Size: 389 1540 1291 4545
Fragments arranged by size:
4545 1540 1291 389
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3366 4054 5869 6770 7032 820
Size: 197 926 1423 688 1815 901 262 1553
Fragments arranged by size:
1815 1553 1423 926 901 688 262 197
SnaBI TAC'GTA
Cuts at: 493 5556 493
Size: 5063 2702
SpeI A'CTAG_T
Cuts at: 152 3889 152
Size: 3737 4028
SphI G_CATG'C
Cuts at: 1132 5123 1132
Size: 3991 3774
SspI AAT'ATT
Cuts at: 5 52 3086 5689 5
Size: 47 3034 2603 2081
Fragments arranged by size:
3034 2603 2081 47
StuI AGG'CCT
Cuts at: 1517 2468 1517
Size: 951 6814
StyI C'CwwG_G
Cuts at: 513 1065 2004 2231 2691 3705 3975 4116 4275
Size: 552 939 227 460 1014 270 141 159
Cuts at: 4275 4329 5204 513
Size: 54 875 3074
Fragments arranged by size:
3074 1014 939 875 552 460 270 227
159 141 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2193 4720 4839 5792 5809 5962 6147
Size: 1255 613 2527 119 953 17 153 185
Cuts at: 6147 6592 325
Size: 445 1498
Fragments arranged by size:
2527 1498 1255 953 613 445 185 153
119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4608 6011 7723
Size: 274 80 33 51 1443 2631 1403 1712
Cuts at: 7723 96
Size: 138
Fragments arranged by size:
2631 1712 1443 1403 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 4619 4651 7725 7757 3521
Size: 32 1066 32 3074 32 3529
Fragments arranged by size:
3529 3074 1066 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3200 1316
Size: 1884 5881
XbaI T'CTAG_A
Cuts at: 5101 5101
Size: 7765
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2852 2852
Size: 7765
XhoI C'TCGA_G
Cuts at: 1943 3366 1943
Size: 1423 6342
XmaI C'CCGG_G
Cuts at: 1870 2259 3799 5090 1870
Size: 389 1540 1291 4545
Fragments arranged by size:
4545 1540 1291 389
XmnI GAAnn'nnTTC
Cuts at: 5894 5894
Size: 7765
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5573 276
Size: 53 83 186 936 4039 2468
Fragments arranged by size:
4039 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BbvCI BstZ17I EcoRV FseI KasI
NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI