Restriction map for
pFN21AB7334
(Circular) (Six-base) MAPSORT of: pFN21AB7334.seq Check: 6835 from: 1 to: 9028
pFN21AB7334 9028 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2178 6838 278
Size: 53 83 186 936 642 4660 2468
Fragments arranged by size:
4660 2468 936 642 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3996 5443 6371 1937
Size: 2059 1447 928 4594
Fragments arranged by size:
4594 2059 1447 928
Acc65I G'GTAC_C
Cuts at: 2496 6349 2496
Size: 3853 5175
AclI AA'CG_TT
Cuts at: 1563 7155 7528 1563
Size: 5592 373 3063
Fragments arranged by size:
5592 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3129 4161 4608 4995 5560 5799 7091 8475
Size: 1497 1032 447 387 565 239 1292 1384
Cuts at: 8475 8743 1632
Size: 268 1917
Fragments arranged by size:
1917 1497 1384 1292 1032 565 447 387
268 239
AfeI AGC'GCT
Cuts at: 2099 2099
Size: 9028
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8831
AflIII A'CryG_T
Cuts at: 1203 4553 7927 8904 1203
Size: 3350 3374 977 1327
Fragments arranged by size:
3374 3350 1327 977
AgeI A'CCGG_T
Cuts at: 7859 7859
Size: 9028
AhdI GACnn_n'nnGTC
Cuts at: 7757 7757
Size: 9028
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5504 1184
Size: 4320 4708
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 8996
AlwNI CAG_nnn'CTG
Cuts at: 2307 5249 6771 8343 2307
Size: 2942 1522 1572 2992
Fragments arranged by size:
2992 2942 1572 1522
ApaLI G'TGCA_C
Cuts at: 7085 8241 7085
Size: 1156 7872
ApoI r'AATT_y
Cuts at: 1493 2411 3059 3364 3475 4118 4467 4495 5327
Size: 918 648 305 111 643 349 28 832
Cuts at: 5327 6337 6563 6715 8824 1493
Size: 1010 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 918 832 648 643 349
305 226 152 111 28
AseI AT'TA_AT
Cuts at: 160 1032 2427 7582 160
Size: 872 1395 5155 1606
Fragments arranged by size:
5155 1606 1395 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9028
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2676 6353 1870
Size: 73 110 623 3677 4545
Fragments arranged by size:
4545 3677 623 110 73
AvrII C'CTAG_G
Cuts at: 5984 5984
Size: 9028
BamHI G'GATC_C
Cuts at: 6358 6725 6358
Size: 367 8661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2496 6349 7804 618
Size: 325 217 627 374 335 3853 1455 1842
Fragments arranged by size:
3853 1842 1455 627 374 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2214 6083 6131 6347 1391
Size: 823 3869 48 216 4072
Fragments arranged by size:
4072 3869 823 216 48
BbeI G_GCGC'C
Cuts at: 2165 2165
Size: 9028
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4292 5667 5737 928
Size: 971 2393 1375 70 4219
Fragments arranged by size:
4219 2393 1375 971 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7219 7253 1903
Size: 34 5282 34 3678
Fragments arranged by size:
5282 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4742 6920 8130 4742
Size: 2178 1210 5640
Fragments arranged by size:
5640 2178 1210
BclI T'GATC_A
Cuts at: 1548 2277 2548 4782 1548
Size: 729 271 2234 5794
Fragments arranged by size:
5794 2234 729 271
BfrBI ATG'CAT
Cuts at: 2574 2574
Size: 9028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5473 7639 136
Size: 107 122 71 631 605 3801 2166 1525
Fragments arranged by size:
3801 2166 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3649 4334 9023 3649
Size: 685 4689 3654
Fragments arranged by size:
4689 3654 685
BlpI GC'TnA_GC
Cuts at: 6445 6445
Size: 9028
Bme1580I G_kGCm'C
Cuts at: 1790 2066 7089 8245 1790
Size: 276 5023 1156 2573
Fragments arranged by size:
5023 2573 1156 276
BmgBI CAC'GTC
Cuts at: 1318 5699 1318
Size: 4381 4647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6759 7717 442
Size: 416 53 83 399 24 5342 958 1753
Fragments arranged by size:
5342 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5795 1056
Size: 4739 4289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4195 4195
Size: 9028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2441 3591 7688 1239
Size: 926 276 1150 4097 2579
Fragments arranged by size:
4097 2579 1150 926 276
Bpu10I CC'TnA_GC
Cuts at: 2139 4713 2139
Size: 2574 6454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3530 5334 7153 8018 8316 3530
Size: 1804 1819 865 298 4242
Fragments arranged by size:
4242 1819 1804 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7691 882
Size: 617 6192 2219
Fragments arranged by size:
6192 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4556 6819 493
Size: 711 3352 2263 2702
Fragments arranged by size:
3352 2702 2263 711
BsaBI GATnn'nnATC
Cuts at: 2403 2511 4784 5958 9022 2403
Size: 108 2273 1174 3064 2409
Fragments arranged by size:
3064 2409 2273 1174 108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2162 2175 6835 7217
Size: 53 83 186 936 629 13 4660 382
Cuts at: 7217 7852 275
Size: 635 1451
Fragments arranged by size:
4660 1451 936 635 629 382 186 83
53 13
BsaWI w'CCGG_w
Cuts at: 5907 6027 7461 7859 8133 8280 8691 5907
Size: 120 1434 398 274 147 411 6244
Fragments arranged by size:
6244 1434 411 398 274 147 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5124 5154 1178
Size: 30 165 30 3721 30 5052
Fragments arranged by size:
5052 3721 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 2447 2717 2786 4094 4316 4646 4764
Size: 845 416 270 69 1308 222 330 118
Cuts at: 4764 5148 5151 5549 5561 5876 5879 5882 5939
Size: 384 3 398 12 315 3 3 57
Cuts at: 5939 5942 6278 1186
Size: 3 336 3936
Fragments arranged by size:
3936 1308 845 416 398 384 336 330
315 270 222 118 69 57 12 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2687 8231 8727 2687
Size: 5544 496 2988
Fragments arranged by size:
5544 2988 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8455
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6478 7239 8267 1540
Size: 275 186 4477 761 1028 2301
Fragments arranged by size:
4477 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 6014 6256 7089 7174 8245 2214
Size: 3800 242 833 85 1071 2997
Fragments arranged by size:
3800 2997 1071 833 242 85
BsmI GAATG_Cn'
Cuts at: 2360 3956 6539 6632 2360
Size: 1596 2583 93 4756
Fragments arranged by size:
4756 2583 1596 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 5909 2459
Size: 3450 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2214 6014 6083 6131 6256 6347
Size: 399 276 148 3800 69 48 125 91
Cuts at: 6347 7089 7174 8245 1391
Size: 742 85 1071 2174
Fragments arranged by size:
3800 2174 1071 742 399 276 148 125
91 85 69 48
BspEI T'CCGG_A
Cuts at: 8691 8691
Size: 9028
BspHI T'CATG_A
Cuts at: 3310 6917 8647 3310
Size: 3607 1730 3691
Fragments arranged by size:
3691 3607 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 6383 844
Size: 1283 4256 3489
Fragments arranged by size:
4256 3489 1283
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 6915 1394
Size: 429 326 4766 3507
Fragments arranged by size:
4766 3507 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 4585 7523 7697 65
Size: 1183 3337 2938 174 1396
Fragments arranged by size:
3337 2938 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5268 7672 7859 1700
Size: 110 3458 2404 187 2869
Fragments arranged by size:
3458 2869 2404 187 110
BsrGI T'GTAC_A
Cuts at: 96 2529 96
Size: 2433 6595
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9028
BssSI C'ACGA_G
Cuts at: 7088 8100 7088
Size: 1012 8016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5249 794
Size: 4455 4573
BstBI TT'CG_AA
Cuts at: 5818 7832 5818
Size: 2014 7014
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9028
BstXI CCAn_nnnn'nTGG
Cuts at: 2465 4070 2465
Size: 1605 7423
BstYI r'GATC_y
Cuts at: 1972 2998 3285 3567 3649 4334 5345 5398 6358
Size: 1026 287 282 82 685 1011 53 960
Cuts at: 6358 6725 7110 7127 8579 8665 8677 9023 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1026 1011 960 685 385 367
346 287 282 86 82 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 3873 3873
Size: 9028
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7537
Fragments arranged by size:
7537 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8289
Fragments arranged by size:
8289 683 56
BtsI GCAGTG_nn'
Cuts at: 3297 4152 4356 4609 6544 7338 7358 3297
Size: 855 204 253 1935 794 20 4967
Fragments arranged by size:
4967 1935 855 794 253 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4675 4710 529
Size: 35 4111 35 4847
Fragments arranged by size:
4847 4111 35 35
DraI TTT'AAA
Cuts at: 6332 6332
Size: 9028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3197 8035 809
Size: 507 1881 4838 1802
Fragments arranged by size:
4838 1881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3976 5143 5465
Size: 54 1425 50 275 18 2146 1167 322
Cuts at: 5465 6475 7364 8993 8
Size: 1010 889 1629 43
Fragments arranged by size:
2146 1629 1425 1167 1010 889 322 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6475 1537
Size: 275 4663 4090
Fragments arranged by size:
4663 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2566 2677 2814 3264 3405 3661 3699 4293
Size: 1213 111 137 450 141 256 38 594
Cuts at: 4293 4425 4623 5307 5526 5681 5748 5868 6042
Size: 132 198 684 219 155 67 120 174
Cuts at: 6042 6255 6958 1353
Size: 213 703 3423
Fragments arranged by size:
3423 1213 703 684 594 450 256 219
213 198 174 155 141 137 132 120
111 67 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5391 7615 7989 8135 387
Size: 1064 138 3802 2224 374 146 1280
Fragments arranged by size:
3802 2224 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2212 2212
Size: 9028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2441 3129 3591 4161 4608 4995
Size: 393 533 276 688 462 570 447 387
Cuts at: 4995 5560 5799 7091 7688 8475 8743 1239
Size: 565 239 1292 597 787 268 1524
Fragments arranged by size:
1524 1292 787 688 597 570 565 533
462 447 393 387 276 268 239
EcoNI CCTnn'n_nnAGG
Cuts at: 4013 4183 4013
Size: 170 8858
EcoO109I rG'GnC_Cy
Cuts at: 3206 5989 3206
Size: 2783 6245
EcoRI G'AATT_C
Cuts at: 5327 6337 6715 5327
Size: 1010 378 7640
Fragments arranged by size:
7640 1010 378
EcoRV GAT'ATC
Cuts at: 4221 5158 4221
Size: 937 8091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6293 6293
Size: 9028
FspI TGC'GCA
Cuts at: 7534 7534
Size: 9028
HaeII r_GCGC'y
Cuts at: 1678 2101 2165 3739 8175 1678
Size: 423 64 1574 4436 2531
Fragments arranged by size:
4436 2531 1574 423 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 3559 3591 4210
Size: 32 135 32 454 32 1501 32 619
Cuts at: 4210 4242 4366 4398 4531 4563 5390 5422 5525
Size: 32 124 32 133 32 827 32 103
Cuts at: 5525 5557 7669 7701 7743 7775 1373
Size: 32 2112 32 42 32 2626
Fragments arranged by size:
2626 2112 1501 827 619 454 135 133
124 103 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4437 5040 6372 6618 669
Size: 988 281 2499 603 1332 246 3079
Fragments arranged by size:
3079 2499 1332 988 603 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9028
HpaI GTT'AAC
Cuts at: 6618 6618
Size: 9028
KasI G'GCGC_C
Cuts at: 2161 2161
Size: 9028
KpnI G_GTAC'C
Cuts at: 2500 6353 2500
Size: 3853 5175
MfeI C'AATT_G
Cuts at: 2463 2501 5091 6627 2463
Size: 38 2590 1536 4864
Fragments arranged by size:
4864 2590 1536 38
MluI A'CGCG_T
Cuts at: 8904 8904
Size: 9028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3254 4208 8142 8326 1305
Size: 1949 954 3934 184 2007
Fragments arranged by size:
3934 2007 1949 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3978 10
Size: 54 1425 343 2146 5060
Fragments arranged by size:
5060 2146 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2569 5952 6986 7345 7504 518
Size: 648 1403 3383 1034 359 159 2042
Fragments arranged by size:
3383 2042 1403 1034 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2110 3325 3881 5246 6444 7121 8269
Size: 418 86 1215 556 1365 1198 677 1148
Cuts at: 8269 8514 1606
Size: 245 2120
Fragments arranged by size:
2120 1365 1215 1198 1148 677 556 418
245 86
NaeI GCC'GGC
Cuts at: 5270 5270
Size: 9028
NarI GG'CG_CC
Cuts at: 2162 2162
Size: 9028
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7537
Fragments arranged by size:
7537 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9028
NgoMIV G'CCGG_C
Cuts at: 5268 5268
Size: 9028
NheI G'CTAG_C
Cuts at: 1052 5791 1052
Size: 4739 4289
NotI GC'GGCC_GC
Cuts at: 6475 6475
Size: 9028
NsiI A_TGCA'T
Cuts at: 2576 2576
Size: 9028
NspI r_CATG'y
Cuts at: 1132 1229 6386 7931 1132
Size: 97 5157 1545 2229
Fragments arranged by size:
5157 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9028
PciI A'CATG_T
Cuts at: 7927 7927
Size: 9028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9028
PmeI GTTT'AAAC
Cuts at: 6332 6332
Size: 9028
PmlI CAC'GTG
Cuts at: 4556 4556
Size: 9028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2117 2149 7097 7129 8610 8642 2117
Size: 32 4948 32 1481 32 2503
Fragments arranged by size:
4948 2503 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3206 5989 3206
Size: 2783 6245
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9028
PsiI TTA'TAA
Cuts at: 6598 6598
Size: 9028
PstI C_TGCA'G
Cuts at: 830 2160 2257 4621 6380 830
Size: 1330 97 2364 1759 3478
Fragments arranged by size:
3478 2364 1759 1330 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9028
PvuII CAG'CTG
Cuts at: 3325 3881 5246 3325
Size: 556 1365 7107
Fragments arranged by size:
7107 1365 556
SacI G_AGCT'C
Cuts at: 2214 2214
Size: 9028
SalI G'TCGA_C
Cuts at: 1936 6370 1936
Size: 4434 4594
SapI GCTCTTCn'nnn_
Cuts at: 2566 2566
Size: 9028
SbfI CC_TGCA'GG
Cuts at: 6380 6380
Size: 9028
ScaI AGT'ACT
Cuts at: 3844 3967 7276 8988 3844
Size: 123 3309 1712 3884
Fragments arranged by size:
3884 3309 1712 123
SfcI C'TryA_G
Cuts at: 826 1045 2156 2253 2802 3418 4617 5065 6091
Size: 219 1111 97 549 616 1199 448 1026
Cuts at: 6091 6376 7511 8192 8383 826
Size: 285 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 1111 1026 681 616 549
448 285 219 191 97
SfoI GGC'GCC
Cuts at: 2163 2163
Size: 9028
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9028
SgrAI Cr'CCGG_yG
Cuts at: 1700 5268 1700
Size: 3568 5460
SmaI CCC'GGG
Cuts at: 1872 2055 6355 1872
Size: 183 4300 4545
Fragments arranged by size:
4545 4300 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3509 5349 7132 8033 8295 820
Size: 197 926 1566 1840 1783 901 262 1553
Fragments arranged by size:
1840 1783 1566 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6819 493
Size: 6326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9028
SphI G_CATG'C
Cuts at: 1132 6386 1132
Size: 5254 3774
SspI AAT'ATT
Cuts at: 5 52 2273 2422 3073 4571 5516 6952 5
Size: 47 2221 149 651 1498 945 1436 2081
Fragments arranged by size:
2221 2081 1498 1436 945 651 149 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9028
StyI C'CwwG_G
Cuts at: 513 1065 2004 5984 6186 6467 513
Size: 552 939 3980 202 281 3074
Fragments arranged by size:
3980 3074 939 552 281 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7055 7072 7225 7410 7855 325
Size: 1255 5475 17 153 185 445 1498
Fragments arranged by size:
5475 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2490 2529 2638
Size: 274 80 33 51 1443 513 39 109
Cuts at: 2638 3842 3959 3965 5719 7274 8986 96
Size: 1204 117 6 1754 1555 1712 138
Fragments arranged by size:
1754 1712 1555 1443 1204 513 274 138
117 109 80 51 39 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6022 6054 6220 6252 8988 9020 6022
Size: 32 166 32 2736 32 6030
Fragments arranged by size:
6030 2736 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9028
XbaI T'CTAG_A
Cuts at: 2815 3847 4261 4349 6364 2815
Size: 1032 414 88 2015 5479
Fragments arranged by size:
5479 2015 1032 414 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3019 4363 4797 3019
Size: 1344 434 7250
Fragments arranged by size:
7250 1344 434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9028
XmaI C'CCGG_G
Cuts at: 1870 2053 6353 1870
Size: 183 4300 4545
Fragments arranged by size:
4545 4300 183
XmnI GAAnn'nnTTC
Cuts at: 3831 5734 7157 3831
Size: 1903 1423 5702
Fragments arranged by size:
5702 1903 1423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2176 6836 276
Size: 53 83 186 936 642 4660 2468
Fragments arranged by size:
4660 2468 936 642 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbvCI BsiWI BstZ17I DraIII
FseI FspAI NruI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI