Restriction map for
pFN21AB7331
(Circular) (Six-base) MAPSORT of: pFN21AB7331.seq Check: 1591 from: 1 to: 7687
pFN21AB7331 7687 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2308 3381 4448 4756 2308
Size: 1073 1067 308 5239
Fragments arranged by size:
5239 1073 1067 308
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5497 278
Size: 53 83 186 936 3961 2468
Fragments arranged by size:
3961 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2835 4836 5030 1937
Size: 898 2001 194 4594
Fragments arranged by size:
4594 2001 898 194
Acc65I G'GTAC_C
Cuts at: 2389 3659 5008 2389
Size: 1270 1349 5068
Fragments arranged by size:
5068 1349 1270
AclI AA'CG_TT
Cuts at: 1563 5814 6187 1563
Size: 4251 373 3063
Fragments arranged by size:
4251 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2640 3191 3613 4640 5750 7134 7402 1632
Size: 1008 551 422 1027 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 1027 1008 551 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7490
AflIII A'CryG_T
Cuts at: 1203 4130 6586 7563 1203
Size: 2927 2456 977 1327
Fragments arranged by size:
2927 2456 1327 977
AgeI A'CCGG_T
Cuts at: 6518 6518
Size: 7687
AhdI GACnn_n'nnGTC
Cuts at: 6416 6416
Size: 7687
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2507 3584 1184
Size: 1323 1077 5287
Fragments arranged by size:
5287 1323 1077
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2151 2183 2151
Size: 32 7655
AlwNI CAG_nnn'CTG
Cuts at: 2544 2900 5430 7002 2544
Size: 356 2530 1572 3229
Fragments arranged by size:
3229 2530 1572 356
ApaI G_GGCC'C
Cuts at: 2481 3999 4486 2481
Size: 1518 487 5682
Fragments arranged by size:
5682 1518 487
ApaLI G'TGCA_C
Cuts at: 4190 5744 6900 4190
Size: 1554 1156 4977
Fragments arranged by size:
4977 1554 1156
ApoI r'AATT_y
Cuts at: 1493 2228 2743 4996 5222 5374 7483 1493
Size: 735 515 2253 226 152 2109 1697
Fragments arranged by size:
2253 2109 1697 735 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6241 160
Size: 872 5209 1606
Fragments arranged by size:
5209 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7687
AvaI C'yCGr_G
Cuts at: 1870 1943 3304 3972 4061 5012 1870
Size: 73 1361 668 89 951 4545
Fragments arranged by size:
4545 1361 951 668 89 73
AvrII C'CTAG_G
Cuts at: 2500 2500
Size: 7687
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3651 3684 3651
Size: 33 7654
BamHI G'GATC_C
Cuts at: 2625 4241 5017 5384 2625
Size: 1616 776 367 4928
Fragments arranged by size:
4928 1616 776 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 3035 3176 3659 4114
Size: 325 217 627 602 646 141 483 455
Cuts at: 4114 4196 4544 4653 4744 5008 6463 618
Size: 82 348 109 91 264 1455 1842
Fragments arranged by size:
1842 1455 646 627 602 483 455 348
325 264 217 141 109 91 82
BanII G_rGCy'C
Cuts at: 1391 2481 2765 3234 3531 3609 3710 3840 3999
Size: 1090 284 469 297 78 101 130 159
Cuts at: 3999 4172 4486 5006 1391
Size: 173 314 520 4072
Fragments arranged by size:
4072 1090 520 469 314 297 284 173
159 130 101 78
BbeI G_GCGC'C
Cuts at: 3180 3180
Size: 7687
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6716
BbvCI CC'TCA_GC
Cuts at: 2267 2786 2267
Size: 519 7168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3762 3796 5878 5912 1903
Size: 34 50 34 1741 34 2082 34 3678
Fragments arranged by size:
3678 2082 1741 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5579 6789 5579
Size: 1210 6477
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2760 4458 6298
Size: 107 122 71 631 605 1088 1698 1840
Cuts at: 6298 136
Size: 1525
Fragments arranged by size:
1840 1698 1525 1088 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7682 7682
Size: 7687
BlpI GC'TnA_GC
Cuts at: 3500 5104 3500
Size: 1604 6083
Bme1580I G_kGCm'C
Cuts at: 1790 2481 2496 3142 3254 3999 4119 4194 4486
Size: 691 15 646 112 745 120 75 292
Cuts at: 4486 4534 4549 4606 4656 5748 6904 1790
Size: 48 15 57 50 1092 1156 2573
Fragments arranged by size:
2573 1156 1092 745 691 646 292 120
112 75 57 50 48 15 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7687
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2398 3739 5418
Size: 416 53 83 399 24 981 1341 1679
Cuts at: 5418 6376 442
Size: 958 1753
Fragments arranged by size:
1753 1679 1341 981 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3724 3724
Size: 7687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 2443 2457 2589 3279 3719 4347 4741
Size: 1119 85 14 132 690 440 628 394
Cuts at: 4741 4834 4841 6347 1239
Size: 93 7 1506 2579
Fragments arranged by size:
2579 1506 1119 690 628 440 394 132
93 85 14 7
Bpu10I CC'TnA_GC
Cuts at: 2148 2267 2786 3068 4182 2148
Size: 119 519 282 1114 5653
Fragments arranged by size:
5653 1114 519 282 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 3365 4493 5812 6677 6975 2243
Size: 1122 1128 1319 865 298 2955
Fragments arranged by size:
2955 1319 1128 1122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4529 6350 882
Size: 617 1167 1863 1821 2219
Fragments arranged by size:
2219 1863 1821 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 5478 493
Size: 711 4274 2702
Fragments arranged by size:
4274 2702 711
BsaBI GATnn'nnATC
Cuts at: 2624 7681 2624
Size: 5057 2630
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 3177 4348 5494
Size: 53 83 186 936 633 1011 1171 1146
Cuts at: 5494 5876 6511 275
Size: 382 635 1451
Fragments arranged by size:
1451 1171 1146 1011 936 635 633 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2428 4135 6120 6518 6792 6939 7350 2428
Size: 1707 1985 398 274 147 411 2765
Fragments arranged by size:
2765 1985 1707 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 2774 2804 1178
Size: 30 165 30 1020 30 321 30 6061
Fragments arranged by size:
6061 1020 321 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2276 2635 2684 1186
Size: 940 150 359 49 6189
Fragments arranged by size:
6189 940 359 150 49
BseYI C'CCAG_C
Cuts at: 2480 2534 3207 4075 4573 6890 7386 2480
Size: 54 673 868 498 2317 496 2781
Fragments arranged by size:
2781 2317 868 673 498 496 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2286 3359 3802 1163
Size: 573 550 1073 443 5048
Fragments arranged by size:
5048 1073 573 550 443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 3847 5137 5898 6926 1540
Size: 275 186 1282 564 1290 761 1028 2301
Fragments arranged by size:
2301 1290 1282 1028 761 564 275 186
BsiHKAI G_wGCw'C
Cuts at: 3531 4194 4341 5748 5833 6904 3531
Size: 663 147 1407 85 1071 4314
Fragments arranged by size:
4314 1407 1071 663 147 85
BsmI GAATG_Cn'
Cuts at: 5198 5291 5198
Size: 93 7594
BsmBI CGTCTCn'nnnn_
Cuts at: 3295 4968 3295
Size: 1673 6014
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2481 2496 2765 3142 3234 3254 3531
Size: 399 691 15 269 377 92 20 277
Cuts at: 3531 3609 3710 3840 3999 4119 4172 4194 4341
Size: 78 101 130 159 120 53 22 147
Cuts at: 4341 4486 4534 4549 4606 4656 5006 5748 5833
Size: 145 48 15 57 50 350 742 85
Cuts at: 5833 6904 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 377 350 277
269 159 147 145 130 120 101 92
85 78 57 53 50 48 22 20
15 15
BspEI T'CCGG_A
Cuts at: 2428 4135 7350 2428
Size: 1707 3215 2765
Fragments arranged by size:
3215 2765 1707
BspHI T'CATG_A
Cuts at: 5576 7306 5576
Size: 1730 5957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2308 3381 3489 4296 4448 4756 5042 844
Size: 1464 1073 108 807 152 308 286 3489
Fragments arranged by size:
3489 1464 1073 807 308 286 152 108
BsrBI CCG'CTC
Cuts at: 1394 1823 5574 1394
Size: 429 3751 3507
Fragments arranged by size:
3751 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3274 3433 3595 4129 4779 6182 6356
Size: 1183 2026 159 162 534 650 1403 174
Cuts at: 6356 65
Size: 1396
Fragments arranged by size:
2026 1403 1396 1183 650 534 174 162
159
BsrFI r'CCGG_y
Cuts at: 1700 1810 3022 3278 6331 6518 1700
Size: 110 1212 256 3053 187 2869
Fragments arranged by size:
3053 2869 1212 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7687
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7687
BssSI C'ACGA_G
Cuts at: 4597 5747 6759 4597
Size: 1150 1012 5525
Fragments arranged by size:
5525 1150 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4751 794
Size: 3957 3730
BstBI TT'CG_AA
Cuts at: 2452 6491 2452
Size: 4039 3648
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7687
BstXI CCAn_nnnn'nTGG
Cuts at: 4325 4765 4325
Size: 440 7247
BstYI r'GATC_y
Cuts at: 1972 2625 4149 4241 4955 5017 5384 5769 5786
Size: 653 1524 92 714 62 367 385 17
Cuts at: 5786 7238 7324 7336 7682 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1524 1452 714 653 385 367 346
92 86 62 17 12
BstZ17I GTA'TAC
Cuts at: 4837 4837
Size: 7687
Bsu36I CC'TnA_GG
Cuts at: 2999 3354 2999
Size: 355 7332
BtgI C'CryG_G
Cuts at: 513 1065 2082 2379 2585 4026 4200 4374 4519
Size: 552 1017 297 206 1441 174 174 145
Cuts at: 4519 513
Size: 3681
Fragments arranged by size:
3681 1441 1017 552 297 206 174 174
145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4158 4826 487
Size: 683 56 2932 668 3348
Fragments arranged by size:
3348 2932 683 668 56
BtsI GCAGTG_nn'
Cuts at: 2276 2496 2927 5203 5997 6017 2276
Size: 220 431 2276 794 20 3946
Fragments arranged by size:
3946 2276 794 431 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2785 2820 529
Size: 35 2221 35 5396
Fragments arranged by size:
5396 2221 35 35
DraI TTT'AAA
Cuts at: 4991 4991
Size: 7687
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2424 6694 809
Size: 507 1108 4270 1802
Fragments arranged by size:
4270 1802 1108 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2937 3280
Size: 54 1425 50 275 18 504 603 343
Cuts at: 3280 3830 5134 6023 7652 8
Size: 550 1304 889 1629 43
Fragments arranged by size:
1629 1425 1304 889 603 550 504 343
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 5134 1537
Size: 275 1468 1854 4090
Fragments arranged by size:
4090 1854 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3876 4160 5617 1353
Size: 2523 284 1457 3423
Fragments arranged by size:
3423 2523 1457 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6274 6648 6794 387
Size: 1064 138 4685 374 146 1280
Fragments arranged by size:
4685 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3529 3529
Size: 7687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 2443 2457 2589 2640 3191 3279
Size: 393 726 85 14 132 51 551 88
Cuts at: 3279 3613 3719 4347 4640 4741 4834 4841 5750
Size: 334 106 628 293 101 93 7 909
Cuts at: 5750 6347 7134 7402 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 909 787 726 628 597 551 393
334 293 268 132 106 101 93 88
85 51 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 3398 3701 2237
Size: 1161 303 6223
Fragments arranged by size:
6223 1161 303
EcoO109I rG'GnC_Cy
Cuts at: 2074 2417 2477 2564 2729 2918 3445 3493 3926
Size: 343 60 87 165 189 527 48 433
Cuts at: 3926 3995 3996 4065 4267 4285 4483 2074
Size: 69 1 69 202 18 198 5278
Fragments arranged by size:
5278 527 433 343 202 198 189 165
87 69 69 60 48 18 1
EcoRI G'AATT_C
Cuts at: 2228 4996 5374 2228
Size: 2768 378 4541
Fragments arranged by size:
4541 2768 378
FspI TGC'GCA
Cuts at: 3920 6193 3920
Size: 2273 5414
HaeII r_GCGC'y
Cuts at: 1678 2048 3180 6834 1678
Size: 370 1132 3654 2531
Fragments arranged by size:
3654 2531 1132 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 2588 2620 2774
Size: 32 135 32 851 32 133 32 154
Cuts at: 2774 2806 4233 4265 4277 4309 4940 4942 4972
Size: 32 1427 32 12 32 631 2 30
Cuts at: 4972 4974 6328 6360 6402 6434 1373
Size: 2 1354 32 42 32 2626
Fragments arranged by size:
2626 1427 1354 851 631 154 135 133
42 32 32 32 32 32 32 32
32 32 30 12 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3730 5031 5277 669
Size: 988 281 1792 1301 246 3079
Fragments arranged by size:
3079 1792 1301 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7687
HpaI GTT'AAC
Cuts at: 5277 5277
Size: 7687
KasI G'GCGC_C
Cuts at: 3176 3176
Size: 7687
KpnI G_GTAC'C
Cuts at: 2393 3663 5012 2393
Size: 1270 1349 5068
Fragments arranged by size:
5068 1349 1270
MfeI C'AATT_G
Cuts at: 5286 5286
Size: 7687
MluI A'CGCG_T
Cuts at: 7563 7563
Size: 7687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4035 4964 6801 6985 1305
Size: 2730 929 1837 184 2007
Fragments arranged by size:
2730 2007 1837 929 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2336 2939 3832 10
Size: 54 1425 343 504 603 893 3865
Fragments arranged by size:
3865 1425 893 603 504 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 3600 3911 4668 5645 6004 6163
Size: 648 1323 1111 311 757 977 359 159
Cuts at: 6163 518
Size: 2042
Fragments arranged by size:
2042 1323 1111 977 757 648 359 311
159
MspA1I CmG'CkG
Cuts at: 1606 2218 3169 3364 3546 3554 3678 3786 4787
Size: 612 951 195 182 8 124 108 1001
Cuts at: 4787 4879 5103 5780 6928 7173 1606
Size: 92 224 677 1148 245 2120
Fragments arranged by size:
2120 1148 1001 951 677 612 245 224
195 182 124 108 92 8
NarI GG'CG_CC
Cuts at: 3177 3177
Size: 7687
NcoI C'CATG_G
Cuts at: 513 1065 2379 4026 4519 513
Size: 552 1314 1647 493 3681
Fragments arranged by size:
3681 1647 1314 552 493
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7687
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7687
NotI GC'GGCC_GC
Cuts at: 5134 5134
Size: 7687
NspI r_CATG'y
Cuts at: 1132 1229 3878 4134 5045 6590 1132
Size: 97 2649 256 911 1545 2229
Fragments arranged by size:
2649 2229 1545 911 256 97
PasI CC'CwG_GG
Cuts at: 1348 2507 2608 3128 3254 1348
Size: 1159 101 520 126 5781
Fragments arranged by size:
5781 1159 520 126 101
PciI A'CATG_T
Cuts at: 4130 6586 4130
Size: 2456 5231
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3026 3098 4161 4237 4520 1108
Size: 1918 72 1063 76 283 4275
Fragments arranged by size:
4275 1918 1063 283 76 72
PfoI T'CCnGG_A
Cuts at: 2566 2880 4324 4497 2566
Size: 314 1444 173 5756
Fragments arranged by size:
5756 1444 314 173
PmeI GTTT'AAAC
Cuts at: 4991 4991
Size: 7687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5756 5788 7269 7301 5756
Size: 32 1481 32 6142
Fragments arranged by size:
6142 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2417 2564 4065 4267 4285 2417
Size: 147 1501 202 18 5819
Fragments arranged by size:
5819 1501 202 147 18
PshAI GACnn'nnGTC
Cuts at: 1613 4131 1613
Size: 2518 5169
PsiI TTA'TAA
Cuts at: 5257 5257
Size: 7687
PspOMI G'GGCC_C
Cuts at: 2477 3995 4482 2477
Size: 1518 487 5682
Fragments arranged by size:
5682 1518 487
PstI C_TGCA'G
Cuts at: 830 3559 4878 5039 830
Size: 2729 1319 161 3478
Fragments arranged by size:
3478 2729 1319 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7687
PvuII CAG'CTG
Cuts at: 3169 3554 3678 4787 4879 3169
Size: 385 124 1109 92 5977
Fragments arranged by size:
5977 1109 385 124 92
SacI G_AGCT'C
Cuts at: 3531 3531
Size: 7687
SalI G'TCGA_C
Cuts at: 1936 5029 1936
Size: 3093 4594
SanDI GG'GwC_CC
Cuts at: 2417 2417
Size: 7687
SapI GCTCTTCn'nnn_
Cuts at: 4160 4160
Size: 7687
SbfI CC_TGCA'GG
Cuts at: 5039 5039
Size: 7687
ScaI AGT'ACT
Cuts at: 3535 3989 5935 7647 3535
Size: 454 1946 1712 3575
Fragments arranged by size:
3575 1946 1712 454
SexAI A'CCwGG_T
Cuts at: 4230 4230
Size: 7687
SfcI C'TryA_G
Cuts at: 826 1045 2778 3313 3555 4687 4874 5035 6170
Size: 219 1733 535 242 1132 187 161 1135
Cuts at: 6170 6851 7042 826
Size: 681 191 1471
Fragments arranged by size:
1733 1471 1135 1132 681 535 242 219
191 187 161
SfoI GGC'GCC
Cuts at: 3178 3178
Size: 7687
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7687
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7687
SmaI CCC'GGG
Cuts at: 1872 3306 3974 4063 5014 1872
Size: 1434 668 89 951 4545
Fragments arranged by size:
4545 1434 951 668 89
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 3344 4508 5791 6692 6954
Size: 197 926 279 1122 1164 1283 901 262
Cuts at: 6954 820
Size: 1553
Fragments arranged by size:
1553 1283 1164 1122 926 901 279 262
197
SnaBI TAC'GTA
Cuts at: 493 5478 493
Size: 4985 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7687
SphI G_CATG'C
Cuts at: 1132 5045 1132
Size: 3913 3774
SspI AAT'ATT
Cuts at: 5 52 5611 5
Size: 47 5559 2081
Fragments arranged by size:
5559 2081 47
StuI AGG'CCT
Cuts at: 1517 2910 3581 1517
Size: 1393 671 5623
Fragments arranged by size:
5623 1393 671
StyI C'CwwG_G
Cuts at: 513 1065 2379 2409 2500 4026 4519 4740 5126
Size: 552 1314 30 91 1526 493 221 386
Cuts at: 5126 513
Size: 3074
Fragments arranged by size:
3074 1526 1314 552 493 386 221 91
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2999 3141 3221 3658 4587 4696 5714
Size: 1255 1419 142 80 437 929 109 1018
Cuts at: 5714 5731 5884 6069 6514 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1419 1255 1018 929 445 437 185
153 142 109 80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3533 3987 5933
Size: 274 80 33 51 1443 1556 454 1946
Cuts at: 5933 7645 96
Size: 1712 138
Fragments arranged by size:
1946 1712 1556 1443 454 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2268 2300 2302 2334 7647 7679 2268
Size: 32 2 32 5313 32 2276
Fragments arranged by size:
5313 2276 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7687
XbaI T'CTAG_A
Cuts at: 5023 5023
Size: 7687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2564 3155 3652 4220 4372 2377
Size: 187 591 497 568 152 5692
Fragments arranged by size:
5692 591 568 497 187 152
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7687
XmaI C'CCGG_G
Cuts at: 1870 3304 3972 4061 5012 1870
Size: 1434 668 89 951 4545
Fragments arranged by size:
4545 1434 951 668 89
XmnI GAAnn'nnTTC
Cuts at: 5816 5816
Size: 7687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5495 276
Size: 53 83 186 936 3961 2468
Fragments arranged by size:
3961 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BsiWI DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SfiI SrfI SwaI