Restriction map for
pFN21AB7330
(Circular) (Six-base) MAPSORT of: pFN21AB7330.seq Check: 6927 from: 1 to: 7321
pFN21AB7330 7321 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3705 5131 278
Size: 53 83 186 936 2169 1426 2468
Fragments arranged by size:
2468 2169 1426 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2034 4044 4664 1937
Size: 97 2010 620 4594
Fragments arranged by size:
4594 2010 620 97
Acc65I G'GTAC_C
Cuts at: 2463 4642 2463
Size: 2179 5142
AclI AA'CG_TT
Cuts at: 1563 5448 5821 1563
Size: 3885 373 3063
Fragments arranged by size:
3885 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2978 4393 4402 5384 6768 7036 1632
Size: 452 894 1415 9 982 1384 268 1917
Fragments arranged by size:
1917 1415 1384 982 894 452 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7124
AflIII A'CryG_T
Cuts at: 1203 2298 4359 6220 7197 1203
Size: 1095 2061 1861 977 1327
Fragments arranged by size:
2061 1861 1327 1095 977
AgeI A'CCGG_T
Cuts at: 6152 6152
Size: 7321
AhdI GACnn_n'nnGTC
Cuts at: 3739 6050 3739
Size: 2311 5010
AleI CACnn'nnGTG
Cuts at: 1166 2809 1166
Size: 1643 5678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2470 3086 1184
Size: 1286 616 5419
Fragments arranged by size:
5419 1286 616
AlwNI CAG_nnn'CTG
Cuts at: 2620 5064 6636 2620
Size: 2444 1572 3305
Fragments arranged by size:
3305 2444 1572
ApaI G_GGCC'C
Cuts at: 2380 3276 3794 3827 4557 4614 2380
Size: 896 518 33 730 57 5087
Fragments arranged by size:
5087 896 730 518 57 33
ApaLI G'TGCA_C
Cuts at: 5378 6534 5378
Size: 1156 6165
ApoI r'AATT_y
Cuts at: 1493 2239 4630 4856 5008 7117 1493
Size: 746 2391 226 152 2109 1697
Fragments arranged by size:
2391 2109 1697 746 226 152
AscI GG'CGCG_CC
Cuts at: 2094 2094
Size: 7321
AseI AT'TA_AT
Cuts at: 160 1032 5875 160
Size: 872 4843 1606
Fragments arranged by size:
4843 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7321
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 2109 3345 3422 3604 3845 3872
Size: 73 101 65 1236 77 182 241 27
Cuts at: 3872 4318 4334 4646 1870
Size: 446 16 312 4545
Fragments arranged by size:
4545 1236 446 312 241 182 101 77
73 65 27 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4525 4558 4525
Size: 33 7288
BamHI G'GATC_C
Cuts at: 4651 5018 4651
Size: 367 6954
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2463 2651 3442 3503
Size: 325 217 627 405 271 188 791 61
Cuts at: 3503 4003 4111 4190 4477 4642 6097 618
Size: 500 108 79 287 165 1455 1842
Fragments arranged by size:
1842 1455 791 627 500 405 325 287
271 217 188 165 108 79 61
BanII G_rGCy'C
Cuts at: 1391 2369 2380 2672 3276 3794 3827 4557 4614
Size: 978 11 292 604 518 33 730 57
Cuts at: 4614 4640 1391
Size: 26 4072
Fragments arranged by size:
4072 978 730 604 518 292 57 33
26 11
BbeI G_GCGC'C
Cuts at: 2196 4007 4115 4481 2196
Size: 1811 108 366 5036
Fragments arranged by size:
5036 1811 366 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3325 4032 928
Size: 971 1426 707 4217
Fragments arranged by size:
4217 1426 971 707
BbvCI CC'TCA_GC
Cuts at: 2165 3185 2165
Size: 1020 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5512 5546 1903
Size: 34 3575 34 3678
Fragments arranged by size:
3678 3575 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5213 6423 5213
Size: 1210 6111
BclI T'GATC_A
Cuts at: 1548 2820 1548
Size: 1272 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3348 5932 136
Size: 107 122 71 631 605 1676 2584 1525
Fragments arranged by size:
2584 1676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7316 7316
Size: 7321
BlpI GC'TnA_GC
Cuts at: 4738 4738
Size: 7321
Bme1580I G_kGCm'C
Cuts at: 1790 2380 3114 3276 3447 3481 3794 3827 4557
Size: 590 734 162 171 34 313 33 730
Cuts at: 4557 4614 5382 6538 1790
Size: 57 768 1156 2573
Fragments arranged by size:
2573 1156 768 734 730 590 313 171
162 57 34 33
BmgBI CAC'GTC
Cuts at: 1318 3694 4362 4597 1318
Size: 2376 668 235 4042
Fragments arranged by size:
4042 2376 668 235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4423 5052 6010
Size: 416 53 83 399 24 3006 629 958
Cuts at: 6010 442
Size: 1753
Fragments arranged by size:
3006 1753 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 3733 2383
Size: 1350 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2494 3055 3133 3197 3322 3475 4063
Size: 1040 215 561 78 64 125 153 588
Cuts at: 4063 4418 5981 1239
Size: 355 1563 2579
Fragments arranged by size:
2579 1563 1040 588 561 355 215 153
125 78 64
Bpu10I CC'TnA_GC
Cuts at: 2165 3185 2165
Size: 1020 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 3179 5446 6311 6609 2471
Size: 708 2267 865 298 3183
Fragments arranged by size:
3183 2267 865 708 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3959 5984 882
Size: 617 2460 2025 2219
Fragments arranged by size:
2460 2219 2025 617
BsaAI yAC'GTr
Cuts at: 493 1204 2299 3081 5112 493
Size: 711 1095 782 2031 2702
Fragments arranged by size:
2702 2031 1095 782 711
BsaBI GATnn'nnATC
Cuts at: 2519 3950 7315 2519
Size: 1431 3365 2525
Fragments arranged by size:
3365 2525 1431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 3702 4004 4112
Size: 53 83 186 936 660 1509 302 108
Cuts at: 4112 4478 5128 5510 6145 275
Size: 366 650 382 635 1451
Fragments arranged by size:
1509 1451 936 660 650 635 382 366
302 186 108 83 53
BsaWI w'CCGG_w
Cuts at: 2595 5754 6152 6426 6573 6984 2595
Size: 3159 398 274 147 411 2932
Fragments arranged by size:
3159 2932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3059 3089 3131 3161 4178
Size: 30 165 30 1656 30 42 30 1017
Cuts at: 4178 4199 4208 4229 4502 4514 4532 4544 1178
Size: 21 9 21 273 12 18 12 3955
Fragments arranged by size:
3955 1656 1017 273 165 42 30 30
30 30 21 21 18 12 12 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4406 1186
Size: 3220 4101
BseYI C'CCAG_C
Cuts at: 2274 2665 3404 3434 3793 3854 4602 6524 7020
Size: 391 739 30 359 61 748 1922 496
Cuts at: 7020 2274
Size: 2575
Fragments arranged by size:
2575 1922 748 739 496 391 359 61
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 3056 3286 1163
Size: 573 294 1026 230 5198
Fragments arranged by size:
5198 1026 573 294 230
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2267 3831 4122 4256 4473 4771
Size: 275 186 266 1564 291 134 217 298
Cuts at: 4771 5532 6560 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1564 1028 761 298 291 275 266
217 186 134
BsiHKAI G_wGCw'C
Cuts at: 2369 2532 5382 5467 6538 2369
Size: 163 2850 85 1071 3152
Fragments arranged by size:
3152 2850 1071 163 85
BsmI GAATG_Cn'
Cuts at: 4832 4925 4832
Size: 93 7228
BsmBI CGTCTCn'nnnn_
Cuts at: 3249 3249
Size: 7321
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2380 2532 2672 3114 3276 3447
Size: 399 579 11 152 140 442 162 171
Cuts at: 3447 3481 3794 3827 4557 4614 4640 5382 5467
Size: 34 313 33 730 57 26 742 85
Cuts at: 5467 6538 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 730 579 442 399 313
171 162 152 140 85 57 34 33
26 11
BspEI T'CCGG_A
Cuts at: 2595 6984 2595
Size: 4389 2932
BspHI T'CATG_A
Cuts at: 5210 6940 5210
Size: 1730 5591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3078 3880 4353 4676 844
Size: 2234 802 473 323 3489
Fragments arranged by size:
3489 2234 802 473 323
BsrBI CCG'CTC
Cuts at: 1394 1823 2049 2263 4252 5208 1394
Size: 429 226 214 1989 956 3507
Fragments arranged by size:
3507 1989 956 429 226 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 4503 5816 5990 65
Size: 1183 1293 1962 1313 174 1396
Fragments arranged by size:
1962 1396 1313 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2132 2460 2558 2640 3722 4000 4574
Size: 110 322 328 98 82 1082 278 574
Cuts at: 4574 5965 6152 1700
Size: 1391 187 2869
Fragments arranged by size:
2869 1391 1082 574 328 322 278 187
110 98 82
BsrGI T'GTAC_A
Cuts at: 96 3117 96
Size: 3021 4300
BssHII G'CGCG_C
Cuts at: 1925 2094 1925
Size: 169 7152
BssSI C'ACGA_G
Cuts at: 2324 4247 4388 5381 6393 2324
Size: 1923 141 993 1012 3252
Fragments arranged by size:
3252 1923 1012 993 141
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2620 3264 794
Size: 1826 644 4851
Fragments arranged by size:
4851 1826 644
BstBI TT'CG_AA
Cuts at: 6125 6125
Size: 7321
BstEII G'GTnAC_C
Cuts at: 1184 3103 3399 1184
Size: 1919 296 5106
Fragments arranged by size:
5106 1919 296
BstXI CCAn_nnnn'nTGG
Cuts at: 3862 3862
Size: 7321
BstYI r'GATC_y
Cuts at: 1972 2436 4651 5018 5403 5420 6872 6958 6970
Size: 464 2215 367 385 17 1452 86 12
Cuts at: 6970 7316 1972
Size: 346 1977
Fragments arranged by size:
2215 1977 1452 464 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2035 2035
Size: 7321
BtgI C'CryG_G
Cuts at: 513 1065 2004 2467 3900 4089 4186 4200 4210
Size: 552 939 463 1433 189 97 14 10
Cuts at: 4210 4380 4473 513
Size: 170 93 3361
Fragments arranged by size:
3361 1433 939 552 463 189 170 97
93 14 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2518 487
Size: 683 56 1292 5290
Fragments arranged by size:
5290 1292 683 56
BtsI GCAGTG_nn'
Cuts at: 2703 4837 5631 5651 2703
Size: 2134 794 20 4373
Fragments arranged by size:
4373 2134 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7286
DraI TTT'AAA
Cuts at: 4625 4625
Size: 7321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6328 809
Size: 507 5012 1802
Fragments arranged by size:
5012 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2264 2553 3724
Size: 54 1425 50 275 18 434 289 1171
Cuts at: 3724 3828 4119 4470 4569 4768 5657 7286 8
Size: 104 291 351 99 199 889 1629 43
Fragments arranged by size:
1629 1425 1171 889 434 351 291 289
275 199 104 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2264 3828 4119 4470 4768 1537
Size: 275 452 1564 291 351 298 4090
Fragments arranged by size:
4090 1564 452 351 298 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2357 2990 5251 1353
Size: 1004 633 2261 3423
Fragments arranged by size:
3423 2261 1004 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3016 3851 4131 4252 5908 6282
Size: 1064 138 1427 835 280 121 1656 374
Cuts at: 6282 6428 387
Size: 146 1280
Fragments arranged by size:
1656 1427 1280 1064 835 374 280 146
138 121
EcoICRI GAG'CTC
Cuts at: 2367 2367
Size: 7321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2279 2494 2978 3055 3133 3197
Size: 393 452 195 215 484 77 78 64
Cuts at: 3197 3322 3475 4063 4393 4402 4418 5384 5981
Size: 125 153 588 330 9 16 966 597
Cuts at: 5981 6768 7036 1239
Size: 787 268 1524
Fragments arranged by size:
1524 966 787 597 588 484 452 393
330 268 215 195 153 125 78 77
64 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4304 4304
Size: 7321
EcoO109I rG'GnC_Cy
Cuts at: 2143 2376 2377 2815 3144 3226 3272 3273 3372
Size: 233 1 438 329 82 46 1 99
Cuts at: 3372 3595 3790 3823 3850 4329 4497 4554 4583
Size: 223 195 33 27 479 168 57 29
Cuts at: 4583 2143
Size: 4881
Fragments arranged by size:
4881 479 438 329 233 223 195 168
99 82 57 46 33 29 27 1
1
EcoRI G'AATT_C
Cuts at: 4630 5008 4630
Size: 378 6943
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 3910 3934 2089
Size: 1821 24 5476
Fragments arranged by size:
5476 1821 24
FspI TGC'GCA
Cuts at: 4226 5827 4226
Size: 1601 5720
FspAI rTGC'GCAy
Cuts at: 4226 4226
Size: 7321
HaeII r_GCGC'y
Cuts at: 1678 2123 2196 3411 4007 4115 4481 6468 1678
Size: 445 73 1215 596 108 366 1987 2531
Fragments arranged by size:
2531 1987 1215 596 445 366 108 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3059 3091 3725 3757 4199
Size: 32 135 32 1487 32 634 32 442
Cuts at: 4199 4231 4502 4514 4534 4546 5962 5994 6036
Size: 32 271 12 20 12 1416 32 42
Cuts at: 6036 6068 1373
Size: 32 2626
Fragments arranged by size:
2626 1487 1416 634 442 271 135 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4045 4665 4911 669
Size: 988 281 2107 620 246 3079
Fragments arranged by size:
3079 2107 988 620 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7321
HpaI GTT'AAC
Cuts at: 4911 4911
Size: 7321
KasI G'GCGC_C
Cuts at: 2192 4003 4111 4477 2192
Size: 1811 108 366 5036
Fragments arranged by size:
5036 1811 366 108
KpnI G_GTAC'C
Cuts at: 2467 4646 2467
Size: 2179 5142
MfeI C'AATT_G
Cuts at: 4920 4920
Size: 7321
MluI A'CGCG_T
Cuts at: 7197 7197
Size: 7321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4557 6435 6619 1305
Size: 3252 1878 184 2007
Fragments arranged by size:
3252 2007 1878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5499
Fragments arranged by size:
5499 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 2546 2809 5279 5638 5797 518
Size: 648 1359 21 263 2470 359 159 2042
Fragments arranged by size:
2470 2042 1359 648 359 263 159 21
MspA1I CmG'CkG
Cuts at: 1606 3166 3175 3943 4413 4461 4737 5414 6562
Size: 1560 9 768 470 48 276 677 1148
Cuts at: 6562 6807 1606
Size: 245 2120
Fragments arranged by size:
2120 1560 1148 768 677 470 276 245
48 9
NaeI GCC'GGC
Cuts at: 2134 2560 3724 4002 2134
Size: 426 1164 278 5453
Fragments arranged by size:
5453 1164 426 278
NarI GG'CG_CC
Cuts at: 2193 4004 4112 4478 2193
Size: 1811 108 366 5036
Fragments arranged by size:
5036 1811 366 108
NcoI C'CATG_G
Cuts at: 513 1065 2004 3900 513
Size: 552 939 1896 3934
Fragments arranged by size:
3934 1896 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7321
NgoMIV G'CCGG_C
Cuts at: 2132 2558 3722 4000 2132
Size: 426 1164 278 5453
Fragments arranged by size:
5453 1164 426 278
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7321
NotI GC'GGCC_GC
Cuts at: 2264 4768 2264
Size: 2504 4817
NspI r_CATG'y
Cuts at: 1132 1229 3739 3940 4043 4679 6224 1132
Size: 97 2510 201 103 636 1545 2229
Fragments arranged by size:
2510 2229 1545 636 201 103 97
PasI CC'CwG_GG
Cuts at: 1348 2566 1348
Size: 1218 6103
PciI A'CATG_T
Cuts at: 6220 6220
Size: 7321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3453 3781 4155 1108
Size: 2345 328 374 4274
Fragments arranged by size:
4274 2345 374 328
PfoI T'CCnGG_A
Cuts at: 2388 2388
Size: 7321
PmeI GTTT'AAAC
Cuts at: 4625 4625
Size: 7321
PmlI CAC'GTG
Cuts at: 3081 3081
Size: 7321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5390 5422 6903 6935 5390
Size: 32 1481 32 5776
Fragments arranged by size:
5776 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3226 3372 3595 3850 4583 3226
Size: 146 223 255 733 5964
Fragments arranged by size:
5964 733 255 223 146
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7321
PsiI TTA'TAA
Cuts at: 4891 4891
Size: 7321
PspOMI G'GGCC_C
Cuts at: 2376 3272 3790 3823 4553 4610 2376
Size: 896 518 33 730 57 5087
Fragments arranged by size:
5087 896 730 518 57 33
PspXI vC'TCGA_Gb
Cuts at: 3422 3422
Size: 7321
PstI C_TGCA'G
Cuts at: 830 2238 2616 2932 3762 4328 4673 830
Size: 1408 378 316 830 566 345 3478
Fragments arranged by size:
3478 1408 830 566 378 345 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7321
PvuII CAG'CTG
Cuts at: 3175 4461 3175
Size: 1286 6035
SacI G_AGCT'C
Cuts at: 2369 2369
Size: 7321
SalI G'TCGA_C
Cuts at: 1936 4043 4663 1936
Size: 2107 620 4594
Fragments arranged by size:
4594 2107 620
SanDI GG'GwC_CC
Cuts at: 3372 4583 3372
Size: 1211 6110
SapI GCTCTTCn'nnn_
Cuts at: 2357 2357
Size: 7321
SbfI CC_TGCA'GG
Cuts at: 2932 4673 2932
Size: 1741 5580
ScaI AGT'ACT
Cuts at: 2426 3089 3716 5569 7281 2426
Size: 663 627 1853 1712 2466
Fragments arranged by size:
2466 1853 1712 663 627
SfcI C'TryA_G
Cuts at: 826 1045 2234 2612 2928 3758 4324 4669 5804
Size: 219 1189 378 316 830 566 345 1135
Cuts at: 5804 6485 6676 826
Size: 681 191 1471
Fragments arranged by size:
1471 1189 1135 830 681 566 378 345
316 219 191
SfoI GGC'GCC
Cuts at: 2194 4005 4113 4479 2194
Size: 1811 108 366 5036
Fragments arranged by size:
5036 1811 366 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7321
SgrAI Cr'CCGG_yG
Cuts at: 1700 4574 1700
Size: 2874 4447
SmaI CCC'GGG
Cuts at: 1872 2111 3606 4320 4648 1872
Size: 239 1495 714 328 4545
Fragments arranged by size:
4545 1495 714 328 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3194 3422 5425 6326 6588
Size: 197 926 543 708 228 2003 901 262
Cuts at: 6588 820
Size: 1553
Fragments arranged by size:
2003 1553 926 901 708 543 262 228
197
SnaBI TAC'GTA
Cuts at: 493 5112 493
Size: 4619 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7321
SphI G_CATG'C
Cuts at: 1132 4679 1132
Size: 3547 3774
SspI AAT'ATT
Cuts at: 5 52 5245 5
Size: 47 5193 2081
Fragments arranged by size:
5193 2081 47
StuI AGG'CCT
Cuts at: 1517 3018 3974 1517
Size: 1501 956 4864
Fragments arranged by size:
4864 1501 956
StyI C'CwwG_G
Cuts at: 513 1065 2004 3360 3819 3900 4434 4760 513
Size: 552 939 1356 459 81 534 326 3074
Fragments arranged by size:
3074 1356 939 552 534 459 326 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3418 3605 3983 5348 5365 5518 5703
Size: 1255 1838 187 378 1365 17 153 185
Cuts at: 5703 6148 325
Size: 445 1498
Fragments arranged by size:
1838 1498 1365 1255 445 378 187 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2754 3087
Size: 274 80 33 51 1443 447 330 333
Cuts at: 3087 3117 3714 5567 7279 96
Size: 30 597 1853 1712 138
Fragments arranged by size:
1853 1712 1443 597 447 333 330 274
138 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4178 4210 7281 7313 4178
Size: 32 3071 32 4186
Fragments arranged by size:
4186 3071 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7321
XbaI T'CTAG_A
Cuts at: 4657 4657
Size: 7321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 4378 4441 2548
Size: 1830 63 5428
Fragments arranged by size:
5428 1830 63
XhoI C'TCGA_G
Cuts at: 1943 3422 1943
Size: 1479 5842
XmaI C'CCGG_G
Cuts at: 1870 2109 3604 4318 4646 1870
Size: 239 1495 714 328 4545
Fragments arranged by size:
4545 1495 714 328 239
XmnI GAAnn'nnTTC
Cuts at: 2504 4103 5450 2504
Size: 1599 1347 4375
Fragments arranged by size:
4375 1599 1347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3703 5129 276
Size: 53 83 186 936 2169 1426 2468
Fragments arranged by size:
2468 2169 1426 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BfrBI BsiWI Bsu36I DraIII
EcoRV FseI NruI NsiI PacI PsrI RsrII SacII
SexAI SfiI SrfI SwaI