Restriction map for
pFN21AB7329
(Circular) (Six-base) MAPSORT of: pFN21AB7329.seq Check: 2665 from: 1 to: 7405
pFN21AB7329 7405 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3498 3498
Size: 7405
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3365 5215 278
Size: 53 83 186 936 1829 1850 2468
Fragments arranged by size:
2468 1850 1829 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 3542 4748 1937
Size: 747 858 1206 4594
Fragments arranged by size:
4594 1206 858 747
Acc65I G'GTAC_C
Cuts at: 4522 4726 4522
Size: 204 7201
AclI AA'CG_TT
Cuts at: 1563 5532 5905 1563
Size: 3969 373 3063
Fragments arranged by size:
3969 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2263 2367 3227 3269 3950 4502 5468 6852
Size: 631 104 860 42 681 552 966 1384
Cuts at: 6852 7120 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 966 860 681 631 552 268
104 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7208
AflIII A'CryG_T
Cuts at: 1203 6304 7281 1203
Size: 5101 977 1327
Fragments arranged by size:
5101 1327 977
AgeI A'CCGG_T
Cuts at: 6236 6236
Size: 7405
AhdI GACnn_n'nnGTC
Cuts at: 6134 6134
Size: 7405
AleI CACnn'nnGTG
Cuts at: 1166 4361 1166
Size: 3195 4210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7405
AlwNI CAG_nnn'CTG
Cuts at: 4367 5148 6720 4367
Size: 781 1572 5052
Fragments arranged by size:
5052 1572 781
ApaI G_GGCC'C
Cuts at: 4595 4595
Size: 7405
ApaLI G'TGCA_C
Cuts at: 5462 6618 5462
Size: 1156 6249
ApoI r'AATT_y
Cuts at: 1493 2560 3163 3784 4714 4940 5092 7201 1493
Size: 1067 603 621 930 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 930 621 603 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2460 5959 160
Size: 872 1428 3499 1606
Fragments arranged by size:
3499 1606 1428 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7405
AvaI C'yCGr_G
Cuts at: 1870 1943 3823 4170 4730 1870
Size: 73 1880 347 560 4545
Fragments arranged by size:
4545 1880 560 347 73
BamHI G'GATC_C
Cuts at: 4735 5102 4735
Size: 367 7038
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4522 4570 4726 6181 618
Size: 325 217 627 2735 48 156 1455 1842
Fragments arranged by size:
2735 1842 1455 627 325 217 156 48
BanII G_rGCy'C
Cuts at: 1391 3382 3674 4595 4724 1391
Size: 1991 292 921 129 4072
Fragments arranged by size:
4072 1991 921 292 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 2201 3215 3365 3721 3807 4345
Size: 971 294 8 1014 150 356 86 538
Cuts at: 4345 928
Size: 3988
Fragments arranged by size:
3988 1014 971 538 356 294 150 86
8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3257 3291 5596 5630 1903
Size: 34 1320 34 2305 34 3678
Fragments arranged by size:
3678 2305 1320 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5297 6507 5297
Size: 1210 6195
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7405
BfrBI ATG'CAT
Cuts at: 2089 2844 2089
Size: 755 6650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6016 136
Size: 107 122 71 631 605 4344 1525
Fragments arranged by size:
4344 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7400 7400
Size: 7405
BlpI GC'TnA_GC
Cuts at: 4822 4822
Size: 7405
Bme1580I G_kGCm'C
Cuts at: 1790 3999 4276 4595 5466 6622 1790
Size: 2209 277 319 871 1156 2573
Fragments arranged by size:
2573 2209 1156 871 319 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4114 4180 5136
Size: 416 53 83 399 24 2697 66 956
Cuts at: 5136 6094 442
Size: 958 1753
Fragments arranged by size:
2697 1753 958 956 416 399 83 66
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7405
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3482
Size: 7405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2837 3933 6065 1239
Size: 802 796 1096 2132 2579
Fragments arranged by size:
2579 2132 1096 802 796
Bpu10I CC'TnA_GC
Cuts at: 2967 2967
Size: 7405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3154 3371 4514 5530 6395 6693 3154
Size: 217 1143 1016 865 298 3866
Fragments arranged by size:
3866 1143 1016 865 298 217
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3567 4225 6068 882
Size: 617 2068 658 1843 2219
Fragments arranged by size:
2219 2068 1843 658 617
BsaAI yAC'GTr
Cuts at: 493 1204 3197 5196 493
Size: 711 1993 1999 2702
Fragments arranged by size:
2702 1999 1993 711
BsaBI GATnn'nnATC
Cuts at: 7399 7399
Size: 7405
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2544 3362 5212 5594
Size: 53 83 186 936 1011 818 1850 382
Cuts at: 5594 6229 275
Size: 635 1451
Fragments arranged by size:
1850 1451 1011 936 818 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3919 5838 6236 6510 6657 7068 3919
Size: 1919 398 274 147 411 4256
Fragments arranged by size:
4256 1919 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3347 3377 4606 4636 1178
Size: 30 165 30 1944 30 1229 30 3947
Fragments arranged by size:
3947 1944 1229 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2259 3362 1186
Size: 1073 1103 5229
Fragments arranged by size:
5229 1103 1073
BseYI C'CCAG_C
Cuts at: 2700 4625 4665 6608 7104 2700
Size: 1925 40 1943 496 3001
Fragments arranged by size:
3001 1943 1925 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3470 4476 4517 1163
Size: 573 1734 1006 41 4051
Fragments arranged by size:
4051 1734 1006 573 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4552 4855 5616 6644 1540
Size: 275 186 2551 303 761 1028 2301
Fragments arranged by size:
2551 2301 1028 761 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 5466 5551 6622 2865
Size: 2601 85 1071 3648
Fragments arranged by size:
3648 2601 1071 85
BsmI GAATG_Cn'
Cuts at: 2089 3894 4916 5009 2089
Size: 1805 1022 93 4485
Fragments arranged by size:
4485 1805 1022 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3389 3389
Size: 7405
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2865 3382 3674 3999 4276 4595 4724
Size: 399 1075 517 292 325 277 319 129
Cuts at: 4724 5466 5551 6622 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1075 1071 742 517 399 325 319
292 277 129 85
BspEI T'CCGG_A
Cuts at: 3919 7068 3919
Size: 3149 4256
BspHI T'CATG_A
Cuts at: 4255 5294 7024 4255
Size: 1039 1730 4636
Fragments arranged by size:
4636 1730 1039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3498 3604 4760 844
Size: 2654 106 1156 3489
Fragments arranged by size:
3489 2654 1156 106
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 4078 4554 5292 1394
Size: 429 230 2025 476 738 3507
Fragments arranged by size:
3507 2025 738 476 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 3010 5900 6074 65
Size: 1183 1762 2890 174 1396
Fragments arranged by size:
2890 1762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4547 6049 6236 1700
Size: 110 2737 1502 187 2869
Fragments arranged by size:
2869 2737 1502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7405
BssSI C'ACGA_G
Cuts at: 5465 6477 5465
Size: 1012 6393
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4181 4367 794
Size: 3387 186 3832
Fragments arranged by size:
3832 3387 186
BstBI TT'CG_AA
Cuts at: 6209 6209
Size: 7405
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7405
BstXI CCAn_nnnn'nTGG
Cuts at: 4042 4396 4042
Size: 354 7051
BstYI r'GATC_y
Cuts at: 1972 2218 3046 3073 3462 3916 4404 4435 4443
Size: 246 828 27 389 454 488 31 8
Cuts at: 4443 4735 5102 5487 5504 6956 7042 7054 7400
Size: 292 367 385 17 1452 86 12 346
Cuts at: 7400 1972
Size: 1977
Fragments arranged by size:
1977 1452 828 488 454 389 385 367
346 292 246 86 31 27 17 12
8
BstZ17I GTA'TAC
Cuts at: 2685 2685
Size: 7405
BtgI C'CryG_G
Cuts at: 513 1065 2004 2779 3999 4476 513
Size: 552 939 775 1220 477 3442
Fragments arranged by size:
3442 1220 939 775 552 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6666
Fragments arranged by size:
6666 683 56
BtsI GCAGTG_nn'
Cuts at: 3318 3561 4299 4356 4368 4574 4921 5715 5735
Size: 243 738 57 12 206 347 794 20
Cuts at: 5735 3318
Size: 4988
Fragments arranged by size:
4988 794 738 347 243 206 57 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7405
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7370
DraI TTT'AAA
Cuts at: 3790 4709 3790
Size: 919 6486
DraIII CAC_nnn'GTG
Cuts at: 4570 4570
Size: 7405
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 6412 809
Size: 507 3164 1932 1802
Fragments arranged by size:
3164 1932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2776 4338 4386
Size: 54 1425 50 275 18 946 1562 48
Cuts at: 4386 4549 4852 5741 7370 8
Size: 163 303 889 1629 43
Fragments arranged by size:
1629 1562 1425 946 889 303 275 163
54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4549 4852 1537
Size: 275 2737 303 4090
Fragments arranged by size:
4090 2737 303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 5335 1353
Size: 965 3017 3423
Fragments arranged by size:
3423 3017 965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4587 5992 6366 6512 387
Size: 1064 138 2998 1405 374 146 1280
Fragments arranged by size:
2998 1405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2263 2367 2837 3227 3269 3933
Size: 393 409 222 104 470 390 42 664
Cuts at: 3933 3950 4502 5468 6065 6852 7120 1239
Size: 17 552 966 597 787 268 1524
Fragments arranged by size:
1524 966 787 664 597 552 470 409
393 390 268 222 104 42 17
EcoNI CCTnn'n_nnAGG
Cuts at: 4599 4599
Size: 7405
EcoO109I rG'GnC_Cy
Cuts at: 4591 4592 4650 4591
Size: 1 58 7346
Fragments arranged by size:
7346 58 1
EcoRI G'AATT_C
Cuts at: 4714 5092 4714
Size: 378 7027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 7405
FspI TGC'GCA
Cuts at: 3893 5911 3893
Size: 2018 5387
HaeII r_GCGC'y
Cuts at: 1678 6552 1678
Size: 4874 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2288 2320 3224
Size: 32 135 32 638 32 46 32 904
Cuts at: 3224 3256 3347 3379 4237 4269 6046 6078 6120
Size: 32 91 32 858 32 1777 32 42
Cuts at: 6120 6152 1373
Size: 32 2626
Fragments arranged by size:
2626 1777 904 858 638 135 91 46
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 4749 4995 669
Size: 988 281 1695 1116 246 3079
Fragments arranged by size:
3079 1695 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7405
HpaI GTT'AAC
Cuts at: 4995 4995
Size: 7405
KpnI G_GTAC'C
Cuts at: 4526 4730 4526
Size: 204 7201
MfeI C'AATT_G
Cuts at: 5004 5004
Size: 7405
MluI A'CGCG_T
Cuts at: 7281 7281
Size: 7405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2996 6519 6703 1305
Size: 1691 3523 184 2007
Fragments arranged by size:
3523 2007 1691 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2778 4340 10
Size: 54 1425 343 946 1562 3075
Fragments arranged by size:
3075 1562 1425 946 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 2595 2613 3335 4254 4361 5363
Size: 648 928 501 18 722 919 107 1002
Cuts at: 5363 5722 5881 518
Size: 359 159 2042
Fragments arranged by size:
2042 1002 928 919 722 648 501 359
159 107 18
MspA1I CmG'CkG
Cuts at: 1606 2302 2635 4319 4821 5498 6646 6891 1606
Size: 696 333 1684 502 677 1148 245 2120
Fragments arranged by size:
2120 1684 1148 696 677 502 333 245
NaeI GCC'GGC
Cuts at: 4549 4549
Size: 7405
NcoI C'CATG_G
Cuts at: 513 1065 2004 2779 4476 513
Size: 552 939 775 1697 3442
Fragments arranged by size:
3442 1697 939 775 552
NdeI CA'TA_TG
Cuts at: 387 3157 387
Size: 2770 4635
NgoMIV G'CCGG_C
Cuts at: 4547 4547
Size: 7405
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7405
NotI GC'GGCC_GC
Cuts at: 4852 4852
Size: 7405
NsiI A_TGCA'T
Cuts at: 2091 2846 2091
Size: 755 6650
NspI r_CATG'y
Cuts at: 1132 1229 3026 4763 6308 1132
Size: 97 1797 1737 1545 2229
Fragments arranged by size:
2229 1797 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4646 1348
Size: 3298 4107
PciI A'CATG_T
Cuts at: 6304 6304
Size: 7405
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4126 1108
Size: 3018 4387
PfoI T'CCnGG_A
Cuts at: 3939 4446 3939
Size: 507 6898
PmeI GTTT'AAAC
Cuts at: 4709 4709
Size: 7405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 6987 7019 5474
Size: 32 1481 32 5860
Fragments arranged by size:
5860 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4650 4650
Size: 7405
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7405
PsiI TTA'TAA
Cuts at: 3437 4975 3437
Size: 1538 5867
PspOMI G'GGCC_C
Cuts at: 4591 4591
Size: 7405
PstI C_TGCA'G
Cuts at: 830 2994 4363 4515 4581 4757 830
Size: 2164 1369 152 66 176 3478
Fragments arranged by size:
3478 2164 1369 176 152 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7405
PvuII CAG'CTG
Cuts at: 2302 2635 2302
Size: 333 7072
SalI G'TCGA_C
Cuts at: 1936 4747 1936
Size: 2811 4594
SanDI GG'GwC_CC
Cuts at: 4650 4650
Size: 7405
SapI GCTCTTCn'nnn_
Cuts at: 2318 2318
Size: 7405
SbfI CC_TGCA'GG
Cuts at: 4757 4757
Size: 7405
ScaI AGT'ACT
Cuts at: 5653 7365 5653
Size: 1712 5693
SexAI A'CCwGG_T
Cuts at: 4566 4566
Size: 7405
SfcI C'TryA_G
Cuts at: 826 1045 2390 2990 3217 4359 4511 4577 4753
Size: 219 1345 600 227 1142 152 66 176
Cuts at: 4753 5888 6569 6760 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1345 1142 1135 681 600 227 219
191 176 152 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7405
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7405
SmaI CCC'GGG
Cuts at: 1872 4732 1872
Size: 2860 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3169 3386 4529 5509 6410 6672
Size: 197 926 1226 217 1143 980 901 262
Cuts at: 6672 820
Size: 1553
Fragments arranged by size:
1553 1226 1143 980 926 901 262 217
197
SnaBI TAC'GTA
Cuts at: 493 5196 493
Size: 4703 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7405
SphI G_CATG'C
Cuts at: 1132 4763 1132
Size: 3631 3774
SspI AAT'ATT
Cuts at: 5 52 3178 5329 5
Size: 47 3126 2151 2081
Fragments arranged by size:
3126 2151 2081 47
StuI AGG'CCT
Cuts at: 1517 4586 1517
Size: 3069 4336
StyI C'CwwG_G
Cuts at: 513 1065 2004 2779 3742 4093 4241 4476 4485
Size: 552 939 775 963 351 148 235 9
Cuts at: 4485 4844 513
Size: 359 3074
Fragments arranged by size:
3074 963 939 775 552 359 351 235
148 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 3774 4679 5432 5449 5602 5787
Size: 1255 1134 1060 905 753 17 153 185
Cuts at: 5787 6232 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1134 1060 905 753 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2129 2176 4146
Size: 274 80 33 51 1443 152 47 1970
Cuts at: 4146 5651 7363 96
Size: 1505 1712 138
Fragments arranged by size:
1970 1712 1505 1443 274 152 138 80
51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2733 2765 4554 4586 4606 4638 7365 7397 2733
Size: 32 1789 32 20 32 2727 32 2741
Fragments arranged by size:
2741 2727 1789 32 32 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7405
XbaI T'CTAG_A
Cuts at: 4741 4741
Size: 7405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2771 3598 4483 4633 2771
Size: 827 885 150 5543
Fragments arranged by size:
5543 885 827 150
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7405
XmaI C'CCGG_G
Cuts at: 1870 4730 1870
Size: 2860 4545
XmnI GAAnn'nnTTC
Cuts at: 3429 3718 3935 5534 3429
Size: 289 217 1599 5300
Fragments arranged by size:
5300 1599 289 217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3363 5213 276
Size: 53 83 186 936 1829 1850 2468
Fragments arranged by size:
2468 1850 1829 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI