Restriction map for
pFN21AB7328
(Circular) (Six-base) MAPSORT of: pFN21AB7328.seq Check: 7212 from: 1 to: 8782
pFN21AB7328 8782 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6592 278
Size: 53 83 186 936 5056 2468
Fragments arranged by size:
5056 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2226 2246 3855 4275 6125 1937
Size: 289 20 1609 420 1850 4594
Fragments arranged by size:
4594 1850 1609 420 289 20
Acc65I G'GTAC_C
Cuts at: 4377 6103 4377
Size: 1726 7056
AclI AA'CG_TT
Cuts at: 1563 6909 7282 1563
Size: 5346 373 3063
Fragments arranged by size:
5346 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 3231 4420 5353 6039 6845 8229 8497
Size: 479 1120 1189 933 686 806 1384 268
Cuts at: 8497 1632
Size: 1917
Fragments arranged by size:
1917 1384 1189 1120 933 806 686 479
268
AfeI AGC'GCT
Cuts at: 4429 4429
Size: 8782
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8585
AflIII A'CryG_T
Cuts at: 1203 2368 4353 7681 8658 1203
Size: 1165 1985 3328 977 1327
Fragments arranged by size:
3328 1985 1327 1165 977
AgeI A'CCGG_T
Cuts at: 7613 7613
Size: 8782
AhdI GACnn_n'nnGTC
Cuts at: 3892 7511 3892
Size: 3619 5163
AleI CACnn'nnGTG
Cuts at: 1166 2189 5261 1166
Size: 1023 3072 4687
Fragments arranged by size:
4687 3072 1023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4724 4756 4724
Size: 32 8750
AlwNI CAG_nnn'CTG
Cuts at: 3299 4443 4996 5692 6044 6525 8097 3299
Size: 1144 553 696 352 481 1572 3984
Fragments arranged by size:
3984 1572 1144 696 553 481 352
ApaI G_GGCC'C
Cuts at: 2548 2787 4494 2548
Size: 239 1707 6836
Fragments arranged by size:
6836 1707 239
ApaLI G'TGCA_C
Cuts at: 6839 7995 6839
Size: 1156 7626
ApoI r'AATT_y
Cuts at: 1493 2354 3243 4268 5252 6091 6317 6469 8578
Size: 861 889 1025 984 839 226 152 2109
Cuts at: 8578 1493
Size: 1697
Fragments arranged by size:
2109 1697 1025 984 889 861 839 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7336 160
Size: 872 6304 1606
Fragments arranged by size:
6304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8782
AvaI C'yCGr_G
Cuts at: 1870 1943 5808 6107 1870
Size: 73 3865 299 4545
Fragments arranged by size:
4545 3865 299 73
AvrII C'CTAG_G
Cuts at: 3583 3583
Size: 8782
BamHI G'GATC_C
Cuts at: 4129 5611 5650 6112 6479 4129
Size: 1482 39 462 367 6432
Fragments arranged by size:
6432 1482 462 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4377 6103 7558 618
Size: 325 217 627 2590 1726 1455 1842
Fragments arranged by size:
2590 1842 1726 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2548 2787 3566 4181 4475 4494 4691 4932
Size: 1157 239 779 615 294 19 197 241
Cuts at: 4932 5815 6101 1391
Size: 883 286 4072
Fragments arranged by size:
4072 1157 883 779 615 294 286 241
239 197 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 2527 3294 3953 4612 5583 928
Size: 971 200 428 767 659 659 971 4127
Fragments arranged by size:
4127 971 971 767 659 659 428 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6973 7007 1903
Size: 34 5036 34 3678
Fragments arranged by size:
5036 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6674 7884 6674
Size: 1210 7572
BclI T'GATC_A
Cuts at: 1548 2937 3706 1548
Size: 1389 769 6624
Fragments arranged by size:
6624 1389 769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7393 136
Size: 107 122 71 631 605 5721 1525
Fragments arranged by size:
5721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2035 3525 4677 8777 2035
Size: 1490 1152 4100 2040
Fragments arranged by size:
4100 2040 1490 1152
BlpI GC'TnA_GC
Cuts at: 5201 6199 5201
Size: 998 7784
Bme1580I G_kGCm'C
Cuts at: 1790 2548 2787 4494 6843 7999 1790
Size: 758 239 1707 2349 1156 2573
Fragments arranged by size:
2573 2349 1707 1156 758 239
BmgBI CAC'GTC
Cuts at: 1318 3925 5974 1318
Size: 2607 2049 4126
Fragments arranged by size:
4126 2607 2049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 6513 7471
Size: 416 53 83 399 24 682 4414 958
Cuts at: 7471 442
Size: 1753
Fragments arranged by size:
4414 1753 958 682 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2485 4397 5834 2347
Size: 138 1912 1437 5295
Fragments arranged by size:
5295 1912 1437 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 3731 4206 4392 5714 7442 1239
Size: 1196 1296 475 186 1322 1728 2579
Fragments arranged by size:
2579 1728 1322 1296 1196 475 186
Bpu10I CC'TnA_GC
Cuts at: 3758 3953 4290 5273 3758
Size: 195 337 983 7267
Fragments arranged by size:
7267 983 337 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4916 6907 7772 8070 4916
Size: 1991 865 298 5628
Fragments arranged by size:
5628 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3771 4460 7445 882
Size: 617 2272 689 2985 2219
Fragments arranged by size:
2985 2272 2219 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 2344 3913 4356 6573 493
Size: 711 1140 1569 443 2217 2702
Fragments arranged by size:
2702 2217 1569 1140 711 443
BsaBI GATnn'nnATC
Cuts at: 2381 8776 2381
Size: 6395 2387
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3906 4602 6589 6971
Size: 53 83 186 936 2373 696 1987 382
Cuts at: 6971 7606 275
Size: 635 1451
Fragments arranged by size:
2373 1987 1451 936 696 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2580 5477 7215 7613 7887 8034 8445 2580
Size: 2897 1738 398 274 147 411 2917
Fragments arranged by size:
2917 2897 1738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4964 4994 1178
Size: 30 165 30 3561 30 4966
Fragments arranged by size:
4966 3561 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3442 3476 3947 4088 4639 4932 4972 5039
Size: 2256 34 471 141 551 293 40 67
Cuts at: 5039 5140 1186
Size: 101 4828
Fragments arranged by size:
4828 2256 551 471 293 141 101 67
40 34
BseYI C'CCAG_C
Cuts at: 2084 4450 5348 7985 8481 2084
Size: 2366 898 2637 496 2385
Fragments arranged by size:
2637 2385 2366 898 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3476 5905 1163
Size: 573 1740 2429 4040
Fragments arranged by size:
4040 2429 1740 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 3900 6232 6993 8021 1540
Size: 275 186 1864 35 2332 761 1028 2301
Fragments arranged by size:
2332 2301 1864 1028 761 275 186 35
BsiHKAI G_wGCw'C
Cuts at: 2667 3566 4296 4932 5068 5815 6843 6928 7999
Size: 899 730 636 136 747 1028 85 1071
Cuts at: 7999 2667
Size: 3450
Fragments arranged by size:
3450 1071 1028 899 747 730 636 136
85
BsiWI C'GTAC_G
Cuts at: 2763 2763
Size: 8782
BsmI GAATG_Cn'
Cuts at: 5309 5562 6293 6386 5309
Size: 253 731 93 7705
Fragments arranged by size:
7705 731 253 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3103 5234 3103
Size: 2131 6651
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 2667 2787 3566 4181 4296 4475
Size: 399 758 119 120 779 615 115 179
Cuts at: 4475 4494 4691 4932 5068 5815 6101 6843 6928
Size: 19 197 241 136 747 286 742 85
Cuts at: 6928 7999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 779 758 747 742 615 399
286 241 197 179 136 120 119 115
85 19
BspEI T'CCGG_A
Cuts at: 5477 8445 5477
Size: 2968 5814
BspHI T'CATG_A
Cuts at: 5100 5706 6671 8401 5100
Size: 606 965 1730 5481
Fragments arranged by size:
5481 1730 965 606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 3885 4116 5677 6137 844
Size: 2089 952 231 1561 460 3489
Fragments arranged by size:
3489 2089 1561 952 460 231
BsrBI CCG'CTC
Cuts at: 1394 1823 3799 5205 6669 1394
Size: 429 1976 1406 1464 3507
Fragments arranged by size:
3507 1976 1464 1406 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3174 3210 4161 7277 7451 65
Size: 1183 1926 36 951 3116 174 1396
Fragments arranged by size:
3116 1926 1396 1183 951 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 2188 2208 2425 4953 7426 7613
Size: 110 242 136 20 217 2528 2473 187
Cuts at: 7613 1700
Size: 2869
Fragments arranged by size:
2869 2528 2473 242 217 187 136 110
20
BsrGI T'GTAC_A
Cuts at: 96 4566 96
Size: 4470 4312
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8782
BssSI C'ACGA_G
Cuts at: 4826 6842 7854 4826
Size: 2016 1012 5754
Fragments arranged by size:
5754 2016 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8782
BstBI TT'CG_AA
Cuts at: 7586 7586
Size: 8782
BstEII G'GTnAC_C
Cuts at: 1184 3976 4956 5134 1184
Size: 2792 980 178 4832
Fragments arranged by size:
4832 2792 980 178
BstXI CCAn_nnnn'nTGG
Cuts at: 2140 4639 4750 2140
Size: 2499 111 6172
Fragments arranged by size:
6172 2499 111
BstYI r'GATC_y
Cuts at: 1972 2035 2060 3525 3657 4129 4677 5611 5650
Size: 63 25 1465 132 472 548 934 39
Cuts at: 5650 6112 6479 6864 6881 8333 8419 8431 8777
Size: 462 367 385 17 1452 86 12 346
Cuts at: 8777 1972
Size: 1977
Fragments arranged by size:
1977 1465 1452 934 548 472 462 385
367 346 132 86 63 39 25 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3129 3900 5262 5353 513
Size: 552 939 1125 771 1362 91 3942
Fragments arranged by size:
3942 1362 1125 939 771 552 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5920 6068 487
Size: 683 56 4694 148 3201
Fragments arranged by size:
4694 3201 683 148 56
BtsI GCAGTG_nn'
Cuts at: 4569 6298 7092 7112 4569
Size: 1729 794 20 6239
Fragments arranged by size:
6239 1729 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8747
DraI TTT'AAA
Cuts at: 6086 6086
Size: 8782
DraIII CAC_nnn'GTG
Cuts at: 2347 2347
Size: 8782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7789 809
Size: 507 6473 1802
Fragments arranged by size:
6473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 2574 2844
Size: 54 1425 50 275 18 558 186 270
Cuts at: 2844 3897 4869 6229 7118 8747 8
Size: 1053 972 1360 889 1629 43
Fragments arranged by size:
1629 1425 1360 1053 972 889 558 275
270 186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3897 6229 1537
Size: 275 2085 2332 4090
Fragments arranged by size:
4090 2332 2085 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2295 4397 4412 5820 6712 1353
Size: 942 2102 15 1408 892 3423
Fragments arranged by size:
3423 2102 1408 942 892 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 7369 7743 7889 387
Size: 1064 138 482 5298 374 146 1280
Fragments arranged by size:
5298 1280 1064 482 374 146 138
EcoICRI GAG'CTC
Cuts at: 3564 4930 5813 3564
Size: 1366 883 6533
Fragments arranged by size:
6533 1366 883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2435 3231 3731 4206 4392 4420
Size: 393 479 324 796 500 475 186 28
Cuts at: 4420 5353 5714 6039 6845 7442 8229 8497 1239
Size: 933 361 325 806 597 787 268 1524
Fragments arranged by size:
1524 933 806 796 787 597 500 479
475 393 361 325 324 268 186 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2119 2929 3749 2119
Size: 810 820 7152
Fragments arranged by size:
7152 820 810
EcoO109I rG'GnC_Cy
Cuts at: 2018 2318 2466 2545 2783 3754 3802 4491 4993
Size: 300 148 79 238 971 48 689 502
Cuts at: 4993 5328 2018
Size: 335 5472
Fragments arranged by size:
5472 971 689 502 335 300 238 148
79 48
EcoRI G'AATT_C
Cuts at: 6091 6469 6091
Size: 378 8404
EcoRV GAT'ATC
Cuts at: 2805 5557 2805
Size: 2752 6030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3943 3943
Size: 8782
FspI TGC'GCA
Cuts at: 7288 7288
Size: 8782
HaeII r_GCGC'y
Cuts at: 1678 2597 4087 4431 7929 1678
Size: 919 1490 344 3498 2531
Fragments arranged by size:
3498 2531 1490 919 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 3102 3134 3878
Size: 32 135 32 699 32 799 32 744
Cuts at: 3878 3910 4457 4489 7423 7455 7497 7529 1373
Size: 32 547 32 2934 32 42 32 2626
Fragments arranged by size:
2934 2626 799 744 699 547 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2247 6126 6372 669
Size: 988 281 309 3879 246 3079
Fragments arranged by size:
3879 3079 988 309 281 246
HindIII A'AGCT_T
Cuts at: 748 3502 748
Size: 2754 6028
HpaI GTT'AAC
Cuts at: 6372 6372
Size: 8782
KpnI G_GTAC'C
Cuts at: 4381 6107 4381
Size: 1726 7056
MfeI C'AATT_G
Cuts at: 6381 6381
Size: 8782
MluI A'CGCG_T
Cuts at: 2368 8658 2368
Size: 6290 2492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3885 4443 5443 7896 8080 1305
Size: 2580 558 1000 2453 184 2007
Fragments arranged by size:
2580 2453 2007 1000 558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 2846 4871 10
Size: 54 1425 343 558 456 2025 3921
Fragments arranged by size:
3921 2025 1425 558 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2408 3669 4592 4637 5013 5261
Size: 648 1023 219 1261 923 45 376 248
Cuts at: 5261 5670 5720 6740 7099 7258 518
Size: 409 50 1020 359 159 2042
Fragments arranged by size:
2042 1261 1023 1020 923 648 409 376
359 248 219 159 50 45
MspA1I CmG'CkG
Cuts at: 1606 3598 5200 5794 6198 6875 8023 8268 1606
Size: 1992 1602 594 404 677 1148 245 2120
Fragments arranged by size:
2120 1992 1602 1148 677 594 404 245
NaeI GCC'GGC
Cuts at: 2054 2054
Size: 8782
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7291
Fragments arranged by size:
7291 939 552
NdeI CA'TA_TG
Cuts at: 387 2251 387
Size: 1864 6918
NgoMIV G'CCGG_C
Cuts at: 2052 2052
Size: 8782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8782
NotI GC'GGCC_GC
Cuts at: 6229 6229
Size: 8782
NspI r_CATG'y
Cuts at: 1132 1229 4553 6140 7685 1132
Size: 97 3324 1587 1545 2229
Fragments arranged by size:
3324 2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4495 1348
Size: 3147 5635
PciI A'CATG_T
Cuts at: 7681 7681
Size: 8782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5343 5528 1108
Size: 4235 185 4362
Fragments arranged by size:
4362 4235 185
PfoI T'CCnGG_A
Cuts at: 4222 4923 4222
Size: 701 8081
PmeI GTTT'AAAC
Cuts at: 6086 6086
Size: 8782
PmlI CAC'GTG
Cuts at: 2344 3913 2344
Size: 1569 7213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6851 6883 8364 8396 6851
Size: 32 1481 32 7237
Fragments arranged by size:
7237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2018 2318 2466 3754 5328 2018
Size: 300 148 1288 1574 5472
Fragments arranged by size:
5472 1574 1288 300 148
PshAI GACnn'nnGTC
Cuts at: 1613 3968 4542 1613
Size: 2355 574 5853
Fragments arranged by size:
5853 2355 574
PsiI TTA'TAA
Cuts at: 6352 6352
Size: 8782
PspOMI G'GGCC_C
Cuts at: 2544 2783 4490 2544
Size: 239 1707 6836
Fragments arranged by size:
6836 1707 239
PstI C_TGCA'G
Cuts at: 830 3603 4800 6134 830
Size: 2773 1197 1334 3478
Fragments arranged by size:
3478 2773 1334 1197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8782
PvuII CAG'CTG
Cuts at: 3598 5200 5794 3598
Size: 1602 594 6586
Fragments arranged by size:
6586 1602 594
SacI G_AGCT'C
Cuts at: 3566 4932 5815 3566
Size: 1366 883 6533
Fragments arranged by size:
6533 1366 883
SalI G'TCGA_C
Cuts at: 1936 2245 6124 1936
Size: 309 3879 4594
Fragments arranged by size:
4594 3879 309
SapI GCTCTTCn'nnn_
Cuts at: 5820 5820
Size: 8782
SbfI CC_TGCA'GG
Cuts at: 6134 6134
Size: 8782
ScaI AGT'ACT
Cuts at: 5161 7030 8742 5161
Size: 1869 1712 5201
Fragments arranged by size:
5201 1869 1712
SfcI C'TryA_G
Cuts at: 826 1045 3599 3989 4796 4881 6130 7265 7946
Size: 219 2554 390 807 85 1249 1135 681
Cuts at: 7946 8137 826
Size: 191 1471
Fragments arranged by size:
2554 1471 1249 1135 807 681 390 219
191 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8782
SgrAI Cr'CCGG_yG
Cuts at: 1700 2188 1700
Size: 488 8294
SmaI CCC'GGG
Cuts at: 1872 6109 1872
Size: 4237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4931 6886 7787 8049 820
Size: 197 926 2988 1955 901 262 1553
Fragments arranged by size:
2988 1955 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6573 493
Size: 6080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8782
SphI G_CATG'C
Cuts at: 1132 6140 1132
Size: 5008 3774
SspI AAT'ATT
Cuts at: 5 52 6706 5
Size: 47 6654 2081
Fragments arranged by size:
6654 2081 47
StuI AGG'CCT
Cuts at: 1517 2330 5175 1517
Size: 813 2845 5124
Fragments arranged by size:
5124 2845 813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2604 3441 3583 4743 5337 6221
Size: 552 939 600 837 142 1160 594 884
Cuts at: 6221 513
Size: 3074
Fragments arranged by size:
3074 1160 939 884 837 600 594 552
142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2000 2146 3293 4532 4552 4985 6809
Size: 1255 420 146 1147 1239 20 433 1824
Cuts at: 6809 6826 6979 7164 7609 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 1239 1147 445 433 420
185 153 146 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4566 5159 7028
Size: 274 80 33 51 1443 2589 593 1869
Cuts at: 7028 8740 96
Size: 1712 138
Fragments arranged by size:
2589 1869 1712 1443 593 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4270 4302 4471 4498 4503 4530 4964 4996 8742
Size: 32 169 27 5 27 434 32 3746
Cuts at: 8742 8774 4270
Size: 32 4278
Fragments arranged by size:
4278 3746 434 169 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8782
XbaI T'CTAG_A
Cuts at: 6118 6118
Size: 8782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2651 3202 4993 5860 2651
Size: 551 1791 867 5573
Fragments arranged by size:
5573 1791 867 551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8782
XmaI C'CCGG_G
Cuts at: 1870 6107 1870
Size: 4237 4545
XmnI GAAnn'nnTTC
Cuts at: 5473 6911 5473
Size: 1438 7344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6590 276
Size: 53 83 186 936 5056 2468
Fragments arranged by size:
5056 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BbvCI BfrBI BstZ17I Bsu36I
FseI FspAI KasI NarI NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI