Restriction map for
pFN21AB7326
(Circular) (Six-base) MAPSORT of: pFN21AB7326.seq Check: 1016 from: 1 to: 8350
pFN21AB7326 8350 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2261 5602 2261
Size: 3341 5009
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6160 278
Size: 53 83 186 936 4624 2468
Fragments arranged by size:
4624 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2025 5693 1937
Size: 88 3668 4594
Fragments arranged by size:
4594 3668 88
Acc65I G'GTAC_C
Cuts at: 5671 5671
Size: 8350
AclI AA'CG_TT
Cuts at: 1563 6477 6850 1563
Size: 4914 373 3063
Fragments arranged by size:
4914 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5152 6413 7797 8065 1632
Size: 3520 1261 1384 268 1917
Fragments arranged by size:
3520 1917 1384 1261 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8153
AflIII A'CryG_T
Cuts at: 1203 3147 4255 7249 8226 1203
Size: 1944 1108 2994 977 1327
Fragments arranged by size:
2994 1944 1327 1108 977
AgeI A'CCGG_T
Cuts at: 7181 7181
Size: 8350
AhdI GACnn_n'nnGTC
Cuts at: 3040 3151 7079 3040
Size: 111 3928 4311
Fragments arranged by size:
4311 3928 111
AleI CACnn'nnGTG
Cuts at: 1166 4910 5306 1166
Size: 3744 396 4210
Fragments arranged by size:
4210 3744 396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2262 2441 4525 1184
Size: 1078 179 2084 5009
Fragments arranged by size:
5009 2084 1078 179
AlwNI CAG_nnn'CTG
Cuts at: 3836 4051 6093 7665 3836
Size: 215 2042 1572 4521
Fragments arranged by size:
4521 2042 1572 215
ApaLI G'TGCA_C
Cuts at: 3806 6407 7563 3806
Size: 2601 1156 4593
Fragments arranged by size:
4593 2601 1156
ApoI r'AATT_y
Cuts at: 1493 2158 2682 2873 3494 3664 3745 5659 5885
Size: 665 524 191 621 170 81 1914 226
Cuts at: 5885 6037 8146 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1914 1697 665 621 524 226 191
170 152 81
AseI AT'TA_AT
Cuts at: 160 1032 4434 5066 6904 160
Size: 872 3402 632 1838 1606
Fragments arranged by size:
3402 1838 1606 872 632
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 4591 5675 1870
Size: 73 274 2374 1084 4545
Fragments arranged by size:
4545 2374 1084 274 73
BamHI G'GATC_C
Cuts at: 2998 5680 6047 2998
Size: 2682 367 5301
Fragments arranged by size:
5301 2682 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 4054 4990 5671 7126
Size: 325 217 627 533 1734 936 681 1455
Cuts at: 7126 618
Size: 1842
Fragments arranged by size:
1842 1734 1455 936 681 627 533 325
217
BanII G_rGCy'C
Cuts at: 1391 3106 5669 1391
Size: 1715 2563 4072
Fragments arranged by size:
4072 2563 1715
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5236 928
Size: 971 3337 4042
Fragments arranged by size:
4042 3337 971
BbvCI CC'TCA_GC
Cuts at: 4230 4269 4230
Size: 39 8311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6541 6575 1903
Size: 34 4604 34 3678
Fragments arranged by size:
4604 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4954 5649 6242 7452 4954
Size: 695 593 1210 5852
Fragments arranged by size:
5852 1210 695 593
BclI T'GATC_A
Cuts at: 1548 3220 1548
Size: 1672 6678
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4063 5321 6961
Size: 107 122 71 631 605 2391 1258 1640
Cuts at: 6961 136
Size: 1525
Fragments arranged by size:
2391 1640 1525 1258 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8345 8345
Size: 8350
BlpI GC'TnA_GC
Cuts at: 5767 5767
Size: 8350
Bme1580I G_kGCm'C
Cuts at: 1790 3810 5226 6411 7567 1790
Size: 2020 1416 1185 1156 2573
Fragments arranged by size:
2573 2020 1416 1185 1156
BmgBI CAC'GTC
Cuts at: 1318 4026 1318
Size: 2708 5642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4153 5469 5492
Size: 416 53 83 399 24 2736 1316 23
Cuts at: 5492 6081 7039 442
Size: 589 958 1753
Fragments arranged by size:
2736 1753 1316 958 589 416 399 83
53 24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2556 3090 3534 7010 1239
Size: 1317 534 444 3476 2579
Fragments arranged by size:
3476 2579 1317 534 444
Bpu10I CC'TnA_GC
Cuts at: 2295 4230 4269 2295
Size: 1935 39 6376
Fragments arranged by size:
6376 1935 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3231 5416 6475 7340 7638 2957
Size: 274 2185 1059 865 298 3669
Fragments arranged by size:
3669 2185 1059 865 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3551 4795 7013 882
Size: 617 2052 1244 2218 2219
Fragments arranged by size:
2219 2218 2052 1244 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 6141 493
Size: 711 1110 3827 2702
Fragments arranged by size:
3827 2702 1110 711
BsaBI GATnn'nnATC
Cuts at: 8344 8344
Size: 8350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6157 6539 7174 275
Size: 53 83 186 936 4624 382 635 1451
Fragments arranged by size:
4624 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6783 7181 7455 7602 8013 6783
Size: 398 274 147 411 7120
Fragments arranged by size:
7120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8125
Fragments arranged by size:
8125 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 4759 1186
Size: 1228 2345 4777
Fragments arranged by size:
4777 2345 1228
BseYI C'CCAG_C
Cuts at: 2370 3889 3979 7553 8049 2370
Size: 1519 90 3574 496 2671
Fragments arranged by size:
3574 2671 1519 496 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 2913 4007 5379 1163
Size: 573 600 577 1094 1372 4134
Fragments arranged by size:
4134 1372 1094 600 577 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5800 6561 7589 1540
Size: 275 186 3799 761 1028 2301
Fragments arranged by size:
3799 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3106 3810 4709 4907 6411 6496 7567 3106
Size: 704 899 198 1504 85 1071 3889
Fragments arranged by size:
3889 1504 1071 899 704 198 85
BsmI GAATG_Cn'
Cuts at: 2798 5144 5861 5954 2798
Size: 2346 717 93 5194
Fragments arranged by size:
5194 2346 717 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3106 3810 4709 4907 5226 5669 6411
Size: 399 1316 704 899 198 319 443 742
Cuts at: 6411 6496 7567 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1316 1071 899 742 704 443 399
319 198 85
BspEI T'CCGG_A
Cuts at: 8013 8013
Size: 8350
BspHI T'CATG_A
Cuts at: 4924 6239 7969 4924
Size: 1315 1730 5305
Fragments arranged by size:
5305 1730 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2261 5602 5705 844
Size: 1417 3341 103 3489
Fragments arranged by size:
3489 3341 1417 103
BsrBI CCG'CTC
Cuts at: 1394 1823 3196 6237 1394
Size: 429 1373 3041 3507
Fragments arranged by size:
3507 3041 1373 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2234 2430 2466 5580 6845 7019 65
Size: 1183 986 196 36 3114 1265 174 1396
Fragments arranged by size:
3114 1396 1265 1183 986 196 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 6994 7181 1700
Size: 110 5184 187 2869
Fragments arranged by size:
5184 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8350
BssSI C'ACGA_G
Cuts at: 6410 7422 6410
Size: 1012 7338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3836 5312 5594 794
Size: 3042 1476 282 3550
Fragments arranged by size:
3550 3042 1476 282
BstBI TT'CG_AA
Cuts at: 7154 7154
Size: 8350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8350
BstXI CCAn_nnnn'nTGG
Cuts at: 2378 5324 5477 2378
Size: 2946 153 5251
Fragments arranged by size:
5251 2946 153
BstYI r'GATC_y
Cuts at: 1972 2998 3999 5189 5680 6047 6432 6449 7901
Size: 1026 1001 1190 491 367 385 17 1452
Cuts at: 7901 7987 7999 8345 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1190 1026 1001 491 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4771 4771
Size: 8350
BtgI C'CryG_G
Cuts at: 513 1065 2004 2126 3811 513
Size: 552 939 122 1685 5052
Fragments arranged by size:
5052 1685 939 552 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 487
Size: 683 56 1163 6448
Fragments arranged by size:
6448 1163 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2925 4608 4630 5118 5313 5866 6660 6680
Size: 660 1683 22 488 195 553 794 20
Cuts at: 6680 2265
Size: 3935
Fragments arranged by size:
3935 1683 794 660 553 488 195 22
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8315
DraI TTT'AAA
Cuts at: 5654 5654
Size: 8350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7357 809
Size: 507 6041 1802
Fragments arranged by size:
6041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2629 2922 3045
Size: 54 1425 50 275 18 799 293 123
Cuts at: 3045 3191 5797 6686 8315 8
Size: 146 2606 889 1629 43
Fragments arranged by size:
2606 1629 1425 889 799 293 275 146
123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5797 1537
Size: 275 3985 4090
Fragments arranged by size:
4090 3985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3348 3484 4203 4342 4635 4818 4879 6280
Size: 1995 136 719 139 293 183 61 1401
Cuts at: 6280 1353
Size: 3423
Fragments arranged by size:
3423 1995 1401 719 293 183 139 136
61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6937 7311 7457 387
Size: 1064 138 5348 374 146 1280
Fragments arranged by size:
5348 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3104 3104
Size: 8350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2556 3090 3534 5152 6413 7010 7797
Size: 393 924 534 444 1618 1261 597 787
Cuts at: 7797 8065 1239
Size: 268 1524
Fragments arranged by size:
1618 1524 1261 924 787 597 534 444
393 268
EcoO109I rG'GnC_Cy
Cuts at: 2366 3976 2366
Size: 1610 6740
EcoRI G'AATT_C
Cuts at: 5659 6037 5659
Size: 378 7972
FspI TGC'GCA
Cuts at: 6856 6856
Size: 8350
HaeII r_GCGC'y
Cuts at: 1678 7497 1678
Size: 5819 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 3026 3058 3137
Size: 32 135 32 857 32 565 32 79
Cuts at: 3137 3169 3180 3212 3868 3900 4647 4679 6991
Size: 32 11 32 656 32 747 32 2312
Cuts at: 6991 7023 7065 7097 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2312 857 747 656 565 135 79
42 32 32 32 32 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 5694 5940 669
Size: 988 281 3756 246 3079
Fragments arranged by size:
3756 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4695 5238 748
Size: 3947 543 3860
Fragments arranged by size:
3947 3860 543
HpaI GTT'AAC
Cuts at: 5940 5940
Size: 8350
KpnI G_GTAC'C
Cuts at: 5675 5675
Size: 8350
MfeI C'AATT_G
Cuts at: 5949 5949
Size: 8350
MluI A'CGCG_T
Cuts at: 8226 8226
Size: 8350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 3314 4027 4352 4886 5477 5645 7464
Size: 1080 929 713 325 534 591 168 1819
Cuts at: 7464 7648 1305
Size: 184 2007
Fragments arranged by size:
2007 1819 1080 929 713 591 534 325
184 168
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2631 2924 10
Size: 54 1425 343 799 293 5436
Fragments arranged by size:
5436 1425 799 343 293 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3146 3160 3443 4910 5306 5475 6308
Size: 648 1980 14 283 1467 396 169 833
Cuts at: 6308 6667 6826 518
Size: 359 159 2042
Fragments arranged by size:
2042 1980 1467 833 648 396 359 283
169 159 14
MspA1I CmG'CkG
Cuts at: 1606 3554 3983 4564 4973 5766 6443 7591 7836
Size: 1948 429 581 409 793 677 1148 245
Cuts at: 7836 1606
Size: 2120
Fragments arranged by size:
2120 1948 1148 793 677 581 429 409
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3811 513
Size: 552 939 1807 5052
Fragments arranged by size:
5052 1807 939 552
NdeI CA'TA_TG
Cuts at: 387 2035 5034 387
Size: 1648 2999 3703
Fragments arranged by size:
3703 2999 1648
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8350
NotI GC'GGCC_GC
Cuts at: 5797 5797
Size: 8350
NspI r_CATG'y
Cuts at: 1132 1229 3151 3434 4259 5315 5708 7253 1132
Size: 97 1922 283 825 1056 393 1545 2229
Fragments arranged by size:
2229 1922 1545 1056 825 393 283 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8350
PciI A'CATG_T
Cuts at: 3147 4255 7249 3147
Size: 1108 2994 4248
Fragments arranged by size:
4248 2994 1108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8350
PfoI T'CCnGG_A
Cuts at: 2109 2109
Size: 8350
PmeI GTTT'AAAC
Cuts at: 5654 5654
Size: 8350
PmlI CAC'GTG
Cuts at: 2314 2314
Size: 8350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6419 6451 7932 7964 6419
Size: 32 1481 32 6805
Fragments arranged by size:
6805 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8350
PsiI TTA'TAA
Cuts at: 4176 5460 5920 4176
Size: 1284 460 6606
Fragments arranged by size:
6606 1284 460
PspXI vC'TCGA_Gb
Cuts at: 2217 2217
Size: 8350
PstI C_TGCA'G
Cuts at: 830 2272 3828 4603 5113 5702 830
Size: 1442 1556 775 510 589 3478
Fragments arranged by size:
3478 1556 1442 775 589 510
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8350
PvuII CAG'CTG
Cuts at: 3554 3983 4973 3554
Size: 429 990 6931
Fragments arranged by size:
6931 990 429
SacI G_AGCT'C
Cuts at: 3106 3106
Size: 8350
SalI G'TCGA_C
Cuts at: 1936 5692 1936
Size: 3756 4594
SapI GCTCTTCn'nnn_
Cuts at: 3348 4818 3348
Size: 1470 6880
SbfI CC_TGCA'GG
Cuts at: 5702 5702
Size: 8350
ScaI AGT'ACT
Cuts at: 2752 3727 4753 6598 8310 2752
Size: 975 1026 1845 1712 2792
Fragments arranged by size:
2792 1845 1712 1026 975
SfcI C'TryA_G
Cuts at: 826 1045 2268 3097 3824 3995 4599 5109 5698
Size: 219 1223 829 727 171 604 510 589
Cuts at: 5698 6833 7514 7705 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1223 1135 829 727 681 604 589
510 219 191 171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8350
SmaI CCC'GGG
Cuts at: 1872 5677 1872
Size: 3805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2217 2972 3246 5431 6454 7355
Size: 197 926 274 755 274 2185 1023 901
Cuts at: 7355 7617 820
Size: 262 1553
Fragments arranged by size:
2185 1553 1023 926 901 755 274 274
262 197
SnaBI TAC'GTA
Cuts at: 493 6141 493
Size: 5648 2702
SpeI A'CTAG_T
Cuts at: 152 5252 152
Size: 5100 3250
SphI G_CATG'C
Cuts at: 1132 5315 5708 1132
Size: 4183 393 3774
Fragments arranged by size:
4183 3774 393
SspI AAT'ATT
Cuts at: 5 52 2986 4717 6274 5
Size: 47 2934 1731 1557 2081
Fragments arranged by size:
2934 2081 1731 1557 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8350
StyI C'CwwG_G
Cuts at: 513 1065 2004 3210 3811 5789 513
Size: 552 939 1206 601 1978 3074
Fragments arranged by size:
3074 1978 1206 939 601 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4819 6377 6394 6547 6732 7177 325
Size: 1255 3239 1558 17 153 185 445 1498
Fragments arranged by size:
3239 1558 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2557 2750 3725
Size: 274 80 33 51 1443 580 193 975
Cuts at: 3725 4751 5550 6596 8308 96
Size: 1026 799 1046 1712 138
Fragments arranged by size:
1712 1443 1046 1026 975 799 580 274
193 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2472 2504 3984 4016 8310 8342 2472
Size: 32 1480 32 4294 32 2480
Fragments arranged by size:
4294 2480 1480 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4027 1316
Size: 2711 5639
XbaI T'CTAG_A
Cuts at: 3595 4684 5686 3595
Size: 1089 1002 6259
Fragments arranged by size:
6259 1089 1002
XhoI C'TCGA_G
Cuts at: 1943 2217 1943
Size: 274 8076
XmaI C'CCGG_G
Cuts at: 1870 5675 1870
Size: 3805 4545
XmnI GAAnn'nnTTC
Cuts at: 2796 3180 6479 2796
Size: 384 3299 4667
Fragments arranged by size:
4667 3299 384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6158 276
Size: 53 83 186 936 4624 2468
Fragments arranged by size:
4624 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI