Restriction map for
pFN21AB7324
(Circular) (Six-base) MAPSORT of: pFN21AB7324.seq Check: 2125 from: 1 to: 8542
pFN21AB7324 8542 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4486 5370 5406 4486
Size: 884 36 7622
Fragments arranged by size:
7622 884 36
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6352 278
Size: 53 83 186 936 4816 2468
Fragments arranged by size:
4816 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5885 1937
Size: 3948 4594
Acc65I G'GTAC_C
Cuts at: 5863 5863
Size: 8542
AclI AA'CG_TT
Cuts at: 1563 6669 7042 1563
Size: 5106 373 3063
Fragments arranged by size:
5106 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2254 3133 3958 4193 5005 5396 6605 7989
Size: 622 879 825 235 812 391 1209 1384
Cuts at: 7989 8257 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1209 879 825 812 622 391
268 235
AfeI AGC'GCT
Cuts at: 3633 5676 3633
Size: 2043 6499
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8345
AflIII A'CryG_T
Cuts at: 1203 4230 5097 7441 8418 1203
Size: 3027 867 2344 977 1327
Fragments arranged by size:
3027 2344 1327 977 867
AgeI A'CCGG_T
Cuts at: 7373 7373
Size: 8542
AhdI GACnn_n'nnGTC
Cuts at: 7271 7271
Size: 8542
AleI CACnn'nnGTG
Cuts at: 1166 2324 4733 4754 1166
Size: 1158 2409 21 4954
Fragments arranged by size:
4954 2409 1158 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4448 4772 1184
Size: 3264 324 4954
Fragments arranged by size:
4954 3264 324
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 3549 3581 3770 3802 3831 3863 3892
Size: 32 1163 32 189 32 29 32 29
Cuts at: 3892 3924 2354
Size: 32 6972
Fragments arranged by size:
6972 1163 189 32 32 32 32 32
29 29
AlwNI CAG_nnn'CTG
Cuts at: 3070 3372 3473 4171 4558 5719 6285 7857 3070
Size: 302 101 698 387 1161 566 1572 3755
Fragments arranged by size:
3755 1572 1161 698 566 387 302 101
ApaI G_GGCC'C
Cuts at: 2144 2718 3401 4142 5243 5685 2144
Size: 574 683 741 1101 442 5001
Fragments arranged by size:
5001 1101 741 683 574 442
ApaLI G'TGCA_C
Cuts at: 2094 4234 4286 5357 6599 7755 2094
Size: 2140 52 1071 1242 1156 2881
Fragments arranged by size:
2881 2140 1242 1156 1071 52
ApoI r'AATT_y
Cuts at: 1493 4336 5851 6077 6229 8338 1493
Size: 2843 1515 226 152 2109 1697
Fragments arranged by size:
2843 2109 1697 1515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7096 160
Size: 872 6064 1606
Fragments arranged by size:
6064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8542
AvaI C'yCGr_G
Cuts at: 1870 1943 2163 2322 2685 2845 3042 3655 4135
Size: 73 220 159 363 160 197 613 480
Cuts at: 4135 4805 4832 5462 5612 5678 5867 1870
Size: 670 27 630 150 66 189 4545
Fragments arranged by size:
4545 670 630 613 480 363 220 197
189 160 159 150 73 66 27
AvrII C'CTAG_G
Cuts at: 2479 2479
Size: 8542
BamHI G'GATC_C
Cuts at: 4848 5872 6239 4848
Size: 1024 367 7151
Fragments arranged by size:
7151 1024 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 2654 2801 3046 3354
Size: 325 217 627 792 75 147 245 308
Cuts at: 3354 4691 4748 4767 4859 4886 5036 5375 5406
Size: 1337 57 19 92 27 150 339 31
Cuts at: 5406 5445 5786 5863 7318 618
Size: 39 341 77 1455 1842
Fragments arranged by size:
1842 1455 1337 792 627 341 339 325
308 245 217 150 147 92 77 75
57 39 31 27 19
BanII G_rGCy'C
Cuts at: 1391 2144 2253 2412 2684 2718 2846 2933 3011
Size: 753 109 159 272 34 128 87 78
Cuts at: 3011 3401 3768 4134 4142 4683 5243 5463 5633
Size: 390 367 366 8 541 560 220 170
Cuts at: 5633 5685 5861 1391
Size: 52 176 4072
Fragments arranged by size:
4072 753 560 541 390 367 366 272
220 176 170 159 128 109 87 78
52 34 8
BbeI G_GCGC'C
Cuts at: 4695 4771 4890 5040 5379 4695
Size: 76 119 150 339 7858
Fragments arranged by size:
7858 339 150 119 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2260 4181 5011 928
Size: 971 361 1921 830 4459
Fragments arranged by size:
4459 1921 971 830 361
BbvCI CC'TCA_GC
Cuts at: 3791 3852 3913 3791
Size: 61 61 8420
Fragments arranged by size:
8420 61 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6733 6767 1903
Size: 34 50 34 4712 34 3678
Fragments arranged by size:
4712 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 6434 7644 3572
Size: 2862 1210 4470
Fragments arranged by size:
4470 2862 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2688 2780
Size: 107 122 71 631 605 437 579 92
Cuts at: 2780 3386 3405 4317 4970 4979 5034 5165 5415
Size: 606 19 912 653 9 55 131 250
Cuts at: 5415 5496 7153 136
Size: 81 1657 1525
Fragments arranged by size:
1657 1525 912 653 631 606 605 579
437 250 131 122 107 92 81 71
55 19 9
BglII A'GATC_T
Cuts at: 8537 8537
Size: 8542
BlpI GC'TnA_GC
Cuts at: 3422 5959 3422
Size: 2537 6005
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2144 2659 2718 3049 3401 4142 4238
Size: 308 46 515 59 331 352 741 96
Cuts at: 4238 4290 4751 5243 5361 5388 5685 6603 7759
Size: 52 461 492 118 27 297 918 1156
Cuts at: 7759 1790
Size: 2573
Fragments arranged by size:
2573 1156 918 741 515 492 461 352
331 308 297 118 96 59 52 46
27
BmgBI CAC'GTC
Cuts at: 1318 5098 1318
Size: 3780 4762
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5216 6273 7231
Size: 416 53 83 399 24 3799 1057 958
Cuts at: 7231 442
Size: 1753
Fragments arranged by size:
3799 1753 1057 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2313 2313
Size: 8542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2666 2716 2762 2812 2956 3019 7202 1239
Size: 1427 50 46 50 144 63 4183 2579
Fragments arranged by size:
4183 2579 1427 144 63 50 50 46
Bpu10I CC'TnA_GC
Cuts at: 3791 3852 3913 4562 3791
Size: 61 61 649 7771
Fragments arranged by size:
7771 649 61 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6667 7532 7830 6667
Size: 865 298 7379
Fragments arranged by size:
7379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3239 7205 882
Size: 617 1740 3966 2219
Fragments arranged by size:
3966 2219 1740 617
BsaAI yAC'GTr
Cuts at: 493 1204 3601 4756 4968 5356 6333 493
Size: 711 2397 1155 212 388 977 2702
Fragments arranged by size:
2702 2397 1155 977 711 388 212
BsaBI GATnn'nnATC
Cuts at: 8536 8536
Size: 8542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2455 2788 4692 4768
Size: 53 83 186 936 922 333 1904 76
Cuts at: 4768 4887 5037 5139 5157 5376 6349 6731 7366
Size: 119 150 102 18 219 973 382 635
Cuts at: 7366 275
Size: 1451
Fragments arranged by size:
1904 1451 973 936 922 635 382 333
219 186 150 119 102 83 76 53
18
BsaWI w'CCGG_w
Cuts at: 6975 7373 7647 7794 8205 6975
Size: 398 274 147 411 7312
Fragments arranged by size:
7312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3454 3484 3549 3579 5450
Size: 30 165 30 2051 30 65 30 1871
Cuts at: 5450 5480 1178
Size: 30 4240
Fragments arranged by size:
4240 2051 1871 165 65 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2909 3221 3449 4823 5384 5471 1186
Size: 1234 489 312 228 1374 561 87 4257
Fragments arranged by size:
4257 1374 1234 561 489 312 228 87
BseYI C'CCAG_C
Cuts at: 2150 2411 2591 2650 2667 2737 3225 3670 4682
Size: 261 180 59 17 70 488 445 1012
Cuts at: 4682 4705 5173 5449 5531 5607 5735 7745 8241
Size: 23 468 276 82 76 128 2010 496
Cuts at: 8241 2150
Size: 2451
Fragments arranged by size:
2451 2010 1012 496 488 468 445 276
261 180 128 82 76 70 59 23
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 2737 3916 4199 4418 4778 5177
Size: 573 706 295 1179 283 219 360 399
Cuts at: 5177 5384 5560 1163
Size: 207 176 4145
Fragments arranged by size:
4145 1179 706 573 399 360 295 283
219 207 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2241 2419 2724 4242 4843 5992
Size: 275 186 240 178 305 1518 601 1149
Cuts at: 5992 6753 7781 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1518 1149 1028 761 601 305 275
240 186 178
BsiHKAI G_wGCw'C
Cuts at: 2098 3077 4042 4134 4185 4238 4290 4911 5070
Size: 979 965 92 51 53 52 621 159
Cuts at: 5070 5361 6603 6688 7759 2098
Size: 291 1242 85 1071 2881
Fragments arranged by size:
2881 1242 1071 979 965 621 291 159
92 85 53 52 51
BsmI GAATG_Cn'
Cuts at: 3986 6053 6146 3986
Size: 2067 93 6382
Fragments arranged by size:
6382 2067 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 2320
Size: 8542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2144 2253 2412 2659 2684 2718
Size: 399 308 46 109 159 247 25 34
Cuts at: 2718 2846 2933 3011 3049 3077 3401 3768 4042
Size: 128 87 78 38 28 324 367 274
Cuts at: 4042 4134 4142 4185 4238 4290 4683 4751 4911
Size: 92 8 43 53 52 393 68 160
Cuts at: 4911 5070 5243 5361 5388 5463 5633 5685 5861
Size: 159 173 118 27 75 170 52 176
Cuts at: 5861 6603 6688 7759 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 367 324 308
274 247 176 173 170 160 159 159
128 118 109 92 87 85 78 75
68 53 52 52 46 43 38 34
28 27 25 8
BspEI T'CCGG_A
Cuts at: 8205 8205
Size: 8542
BspHI T'CATG_A
Cuts at: 6431 8161 6431
Size: 1730 6812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 4486 4773 5268 5370 5406 5897 844
Size: 1559 2083 287 495 102 36 491 3489
Fragments arranged by size:
3489 2083 1559 495 491 287 102 36
BsrBI CCG'CTC
Cuts at: 1394 1823 6429 1394
Size: 429 4606 3507
Fragments arranged by size:
4606 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7037 7211 65
Size: 1183 5789 174 1396
Fragments arranged by size:
5789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 4320 4376 5296 5442 5798 7186
Size: 110 371 2139 56 920 146 356 1388
Cuts at: 7186 7373 1700
Size: 187 2869
Fragments arranged by size:
2869 2139 1388 920 371 356 187 146
110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8542
BssHII G'CGCG_C
Cuts at: 1925 4930 1925
Size: 3005 5537
BssSI C'ACGA_G
Cuts at: 2677 3092 6602 7614 2677
Size: 415 3510 1012 3605
Fragments arranged by size:
3605 3510 1012 415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3473 4558 4877 794
Size: 2679 1085 319 4459
Fragments arranged by size:
4459 2679 1085 319
BstBI TT'CG_AA
Cuts at: 7346 7346
Size: 8542
BstEII G'GTnAC_C
Cuts at: 1184 3749 4498 4726 5602 1184
Size: 2565 749 228 876 4124
Fragments arranged by size:
4124 2565 876 749 228
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2836 3257 3707 5539 2326
Size: 510 421 450 1832 5329
Fragments arranged by size:
5329 1832 510 450 421
BstYI r'GATC_y
Cuts at: 1972 4168 4848 5872 6239 6624 6641 8093 8179
Size: 2196 680 1024 367 385 17 1452 86
Cuts at: 8179 8191 8537 1972
Size: 12 346 1977
Fragments arranged by size:
2196 1977 1452 1024 680 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3432 4114 3432
Size: 682 7860
BtgI C'CryG_G
Cuts at: 513 1065 2014 2718 2757 3250 4051 4221 4843
Size: 552 949 704 39 493 801 170 622
Cuts at: 4843 5216 5598 513
Size: 373 382 3457
Fragments arranged by size:
3457 949 801 704 622 552 493 382
373 170 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7803
Fragments arranged by size:
7803 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 4023 5077 5111 5323 5626 6058 6852 6872
Size: 1925 1054 34 212 303 432 794 20
Cuts at: 6872 2098
Size: 3768
Fragments arranged by size:
3768 1925 1054 794 432 303 212 34
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4089 4124 5267 5302 529
Size: 35 3525 35 1143 35 3769
Fragments arranged by size:
3769 3525 1143 35 35 35
DraI TTT'AAA
Cuts at: 5846 5846
Size: 8542
DraIII CAC_nnn'GTG
Cuts at: 4222 5307 4222
Size: 1085 7457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2122 7549 809
Size: 507 806 5427 1802
Fragments arranged by size:
5427 1802 806 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2416 2721
Size: 54 1425 50 275 18 408 178 305
Cuts at: 2721 3347 3378 3490 3697 4239 4840 4853 4962
Size: 626 31 112 207 542 601 13 109
Cuts at: 4962 5298 5686 5989 6878 8507 8
Size: 336 388 303 889 1629 43
Fragments arranged by size:
1629 1425 889 626 601 542 408 388
336 305 303 275 207 178 112 109
54 50 43 31 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2238 2416 2721 4239 4840 5989 1537
Size: 275 426 178 305 1518 601 1149 4090
Fragments arranged by size:
4090 1518 1149 601 426 305 275 178
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 3527 3996 5066 6472 1353
Size: 951 1223 469 1070 1406 3423
Fragments arranged by size:
3423 1406 1223 1070 951 469
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3557 5357 7129 7503 7649 387
Size: 1064 138 1968 1800 1772 374 146 1280
Fragments arranged by size:
1968 1800 1772 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4132 4132
Size: 8542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2666 2716 2762 2812 2956 3019
Size: 393 622 412 50 46 50 144 63
Cuts at: 3019 3133 3958 4193 5005 5396 6605 7202 7989
Size: 114 825 235 812 391 1209 597 787
Cuts at: 7989 8257 1239
Size: 268 1524
Fragments arranged by size:
1524 1209 825 812 787 622 597 412
393 391 268 235 144 114 63 50
50 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2986 3349 3431 4412 5366 2986
Size: 363 82 981 954 6162
Fragments arranged by size:
6162 981 954 363 82
EcoO109I rG'GnC_Cy
Cuts at: 2140 2194 2378 2991 3322 3369 3481 3652 3661
Size: 54 184 613 331 47 112 171 9
Cuts at: 3661 4078 4510 4588 5240 5816 2140
Size: 417 432 78 652 576 4866
Fragments arranged by size:
4866 652 613 576 432 417 331 184
171 112 78 54 47 9
EcoRI G'AATT_C
Cuts at: 5851 6229 5851
Size: 378 8164
FspI TGC'GCA
Cuts at: 4796 7048 4796
Size: 2252 6290
HaeII r_GCGC'y
Cuts at: 1678 3031 3610 3635 4299 4695 4771 4890 4985
Size: 1353 579 25 664 396 76 119 95
Cuts at: 4985 5040 5379 5678 7689 1678
Size: 55 339 299 2011 2531
Fragments arranged by size:
2531 2011 1353 664 579 396 339 299
119 95 76 55 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3200 3232 4153 4185 7183
Size: 32 135 32 1628 32 921 32 2998
Cuts at: 7183 7215 7257 7289 1373
Size: 32 42 32 2626
Fragments arranged by size:
2998 2626 1628 921 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5886 6132 669
Size: 988 281 3948 246 3079
Fragments arranged by size:
3948 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8542
HpaI GTT'AAC
Cuts at: 6132 6132
Size: 8542
KasI G'GCGC_C
Cuts at: 4691 4767 4886 5036 5375 4691
Size: 76 119 150 339 7858
Fragments arranged by size:
7858 339 150 119 76
KpnI G_GTAC'C
Cuts at: 5867 5867
Size: 8542
MfeI C'AATT_G
Cuts at: 6141 6141
Size: 8542
MluI A'CGCG_T
Cuts at: 4230 8418 4230
Size: 4188 4354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3780 3841 3902 7656 7840 1305
Size: 2475 61 61 3754 184 2007
Fragments arranged by size:
3754 2475 2007 184 61 61
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6720
Fragments arranged by size:
6720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2260 2324 3705 4733 4754 6500 6859
Size: 648 1094 64 1381 1028 21 1746 359
Cuts at: 6859 7018 518
Size: 159 2042
Fragments arranged by size:
2042 1746 1381 1094 1028 648 359 159
64 21
MspA1I CmG'CkG
Cuts at: 1606 2150 2415 2438 3229 3414 3670 3685 3982
Size: 544 265 23 791 185 256 15 297
Cuts at: 3982 4144 4581 4762 4816 4927 5025 5646 5958
Size: 162 437 181 54 111 98 621 312
Cuts at: 5958 6635 7783 8028 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 677 621 544 437 312
297 265 256 245 185 181 162 111
98 54 23 15
NaeI GCC'GGC
Cuts at: 4322 4378 5444 4322
Size: 56 1066 7420
Fragments arranged by size:
7420 1066 56
NarI GG'CG_CC
Cuts at: 4692 4768 4887 5037 5376 4692
Size: 76 119 150 339 7858
Fragments arranged by size:
7858 339 150 119 76
NcoI C'CATG_G
Cuts at: 513 1065 2757 5598 513
Size: 552 1692 2841 3457
Fragments arranged by size:
3457 2841 1692 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8542
NgoMIV G'CCGG_C
Cuts at: 4320 4376 5442 4320
Size: 56 1066 7420
Fragments arranged by size:
7420 1066 56
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8542
NotI GC'GGCC_GC
Cuts at: 5989 5989
Size: 8542
NruI TCG'CGA
Cuts at: 3168 3168
Size: 8542
NspI r_CATG'y
Cuts at: 1132 1229 5121 5900 7445 1132
Size: 97 3892 779 1545 2229
Fragments arranged by size:
3892 2229 1545 779 97
PasI CC'CwG_GG
Cuts at: 1348 2659 2793 4202 4775 5053 5581 1348
Size: 1311 134 1409 573 278 528 4309
Fragments arranged by size:
4309 1409 1311 573 528 278 134
PciI A'CATG_T
Cuts at: 7441 7441
Size: 8542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2150 4420 4461 5008 1108
Size: 1042 2270 41 547 4642
Fragments arranged by size:
4642 2270 1042 547 41
PfoI T'CCnGG_A
Cuts at: 2215 2611 2933 5007 2215
Size: 396 322 2074 5750
Fragments arranged by size:
5750 2074 396 322
PmeI GTTT'AAAC
Cuts at: 5846 5846
Size: 8542
PmlI CAC'GTG
Cuts at: 4968 5356 4968
Size: 388 8154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3549 3581 4895 4927 6611 6643 8124 8156 3549
Size: 32 1314 32 1684 32 1481 32 3935
Fragments arranged by size:
3935 1684 1481 1314 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2378 2991 3481 3652 3661 4588 5816 2378
Size: 613 490 171 9 927 1228 5104
Fragments arranged by size:
5104 1228 927 613 490 171 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8542
PsiI TTA'TAA
Cuts at: 6112 6112
Size: 8542
PspOMI G'GGCC_C
Cuts at: 2140 2714 3397 4138 5239 5681 2140
Size: 574 683 741 1101 442 5001
Fragments arranged by size:
5001 1101 741 683 574 442
PstI C_TGCA'G
Cuts at: 830 3413 3669 3810 3871 3932 4033 4902 5265
Size: 2583 256 141 61 61 101 869 363
Cuts at: 5265 5367 5894 830
Size: 102 527 3478
Fragments arranged by size:
3478 2583 869 527 363 256 141 102
101 61 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8542
PvuII CAG'CTG
Cuts at: 2438 3670 3982 4762 4927 5025 5646 2438
Size: 1232 312 780 165 98 621 5334
Fragments arranged by size:
5334 1232 780 621 312 165 98
SacI G_AGCT'C
Cuts at: 4134 4134
Size: 8542
SalI G'TCGA_C
Cuts at: 1936 5884 1936
Size: 3948 4594
SanDI GG'GwC_CC
Cuts at: 3481 3652 4588 5816 3481
Size: 171 936 1228 6207
Fragments arranged by size:
6207 1228 936 171
SapI GCTCTTCn'nnn_
Cuts at: 2304 3527 2304
Size: 1223 7319
SbfI CC_TGCA'GG
Cuts at: 5265 5367 5894 5265
Size: 102 527 7913
Fragments arranged by size:
7913 527 102
ScaI AGT'ACT
Cuts at: 6790 8502 6790
Size: 1712 6830
SfcI C'TryA_G
Cuts at: 826 1045 3409 3665 3806 3867 3928 4029 4898
Size: 219 2364 256 141 61 61 101 869
Cuts at: 4898 5261 5363 5641 5890 7025 7706 7897 826
Size: 363 102 278 249 1135 681 191 1471
Fragments arranged by size:
2364 1471 1135 869 681 363 278 256
249 219 191 141 102 101 61 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 2780 3386 4970 2780
Size: 606 1584 6352
Fragments arranged by size:
6352 1584 606
SfoI GGC'GCC
Cuts at: 4693 4769 4888 5038 5377 4693
Size: 76 119 150 339 7858
Fragments arranged by size:
7858 339 150 119 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8542
SgrAI Cr'CCGG_yG
Cuts at: 1700 4376 1700
Size: 2676 5866
SmaI CCC'GGG
Cuts at: 1872 2165 2687 3044 3657 5869 1872
Size: 293 522 357 613 2212 4545
Fragments arranged by size:
4545 2212 613 522 357 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6646 7547 7809 820
Size: 197 926 4703 901 262 1553
Fragments arranged by size:
4703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6333 493
Size: 5840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8542
SphI G_CATG'C
Cuts at: 1132 5121 5900 1132
Size: 3989 779 3774
Fragments arranged by size:
3989 3774 779
SspI AAT'ATT
Cuts at: 5 52 6466 5
Size: 47 6414 2081
Fragments arranged by size:
6414 2081 47
StuI AGG'CCT
Cuts at: 1517 3186 3239 3344 3389 5775 1517
Size: 1669 53 105 45 2386 4284
Fragments arranged by size:
4284 2386 1669 105 53 45
StyI C'CwwG_G
Cuts at: 513 1065 2479 2757 3159 3201 3256 3381 5598
Size: 552 1414 278 402 42 55 125 2217
Cuts at: 5598 5754 5981 513
Size: 156 227 3074
Fragments arranged by size:
3074 2217 1414 552 402 278 227 156
125 55 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2586 2642 2963 4788 5247 5463 5594
Size: 1255 1006 56 321 1825 459 216 131
Cuts at: 5594 5621 6569 6586 6739 6924 7369 325
Size: 27 948 17 153 185 445 1498
Fragments arranged by size:
1825 1498 1255 1006 948 459 445 321
216 185 153 131 56 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3266 4737 6788
Size: 274 80 33 51 1443 1289 1471 2051
Cuts at: 6788 8500 96
Size: 1712 138
Fragments arranged by size:
2051 1712 1471 1443 1289 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3717 3749 4032 4064 4090 4122 5049 5081 5468
Size: 32 283 32 26 32 927 32 387
Cuts at: 5468 5500 8502 8534 3717
Size: 32 3002 32 3725
Fragments arranged by size:
3725 3002 927 387 283 32 32 32
32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1316 5015 5713 1316
Size: 3699 698 4145
Fragments arranged by size:
4145 3699 698
XbaI T'CTAG_A
Cuts at: 5878 5878
Size: 8542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4720 4720
Size: 8542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8542
XmaI C'CCGG_G
Cuts at: 1870 2163 2685 3042 3655 5867 1870
Size: 293 522 357 613 2212 4545
Fragments arranged by size:
4545 2212 613 522 357 293
XmnI GAAnn'nnTTC
Cuts at: 6671 6671
Size: 8542
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6350 276
Size: 53 83 186 936 4816 2468
Fragments arranged by size:
4816 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI
FspAI NsiI PacI PspXI PsrI RsrII SacII SexAI
SrfI SwaI