Restriction map for
pFN21AB7322
(Circular) (Six-base) MAPSORT of: pFN21AB7322.seq Check: 3302 from: 1 to: 11761
pFN21AB7322 11761 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4306 5366 4306
Size: 1060 10701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9571 278
Size: 53 83 186 936 8035 2468
Fragments arranged by size:
8035 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 8603 8667 9104 1937
Size: 6666 64 437 4594
Fragments arranged by size:
6666 4594 437 64
Acc65I G'GTAC_C
Cuts at: 9082 9082
Size: 11761
AclI AA'CG_TT
Cuts at: 1563 3771 5991 8542 9888 10261 1563
Size: 2208 2220 2551 1346 373 3063
Fragments arranged by size:
3063 2551 2220 2208 1346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2619 3839 3906 4425 5067 5564 5622
Size: 578 409 1220 67 519 642 497 58
Cuts at: 5622 5853 7604 7815 9824 11208 11476 1632
Size: 231 1751 211 2009 1384 268 1917
Fragments arranged by size:
2009 1917 1751 1384 1220 642 578 519
497 409 268 231 211 67 58
AflII C'TTAA_G
Cuts at: 820 1017 7921 820
Size: 197 6904 4660
Fragments arranged by size:
6904 4660 197
AflIII A'CryG_T
Cuts at: 1203 2941 3139 10660 11637 1203
Size: 1738 198 7521 977 1327
Fragments arranged by size:
7521 1738 1327 977 198
AgeI A'CCGG_T
Cuts at: 10592 10592
Size: 11761
AhdI GACnn_n'nnGTC
Cuts at: 10490 10490
Size: 11761
AleI CACnn'nnGTG
Cuts at: 1166 7256 1166
Size: 6090 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4962 5394 8823 1184
Size: 3778 432 3429 4122
Fragments arranged by size:
4122 3778 3429 432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3756 3788 8527 8559 8560 8592 3756
Size: 32 4739 32 1 32 6925
Fragments arranged by size:
6925 4739 32 32 32 1
AlwNI CAG_nnn'CTG
Cuts at: 2477 2597 4871 4949 5365 5498 9504 11076 2477
Size: 120 2274 78 416 133 4006 1572 3162
Fragments arranged by size:
4006 3162 2274 1572 416 133 120 78
ApaI G_GGCC'C
Cuts at: 2090 8010 2090
Size: 5920 5841
ApaLI G'TGCA_C
Cuts at: 6519 8445 9818 10974 6519
Size: 1926 1373 1156 7306
Fragments arranged by size:
7306 1926 1373 1156
ApoI r'AATT_y
Cuts at: 1493 3200 5252 6694 7129 7822 7840 7945 8128
Size: 1707 2052 1442 435 693 18 105 183
Cuts at: 8128 9070 9296 9448 11557 1493
Size: 942 226 152 2109 1697
Fragments arranged by size:
2109 2052 1707 1697 1442 942 693 435
226 183 152 105 18
AseI AT'TA_AT
Cuts at: 160 1032 3183 6618 10315 160
Size: 872 2151 3435 3697 1606
Fragments arranged by size:
3697 3435 2151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11761
AvaI C'yCGr_G
Cuts at: 1870 1943 3528 4764 8434 9086 1870
Size: 73 1585 1236 3670 652 4545
Fragments arranged by size:
4545 3670 1585 1236 652 73
BamHI G'GATC_C
Cuts at: 6634 9091 9458 6634
Size: 2457 367 8937
Fragments arranged by size:
8937 2457 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 3374 7087 7189 7696
Size: 325 217 627 546 1041 3713 102 507
Cuts at: 7696 8317 9022 9082 10537 618
Size: 621 705 60 1455 1842
Fragments arranged by size:
3713 1842 1455 1041 705 627 621 546
507 325 217 102 60
BanII G_rGCy'C
Cuts at: 1391 2063 2090 2140 4237 4976 5284 7052 8010
Size: 672 27 50 2097 739 308 1768 958
Cuts at: 8010 9080 1391
Size: 1070 4072
Fragments arranged by size:
4072 2097 1768 1070 958 739 672 308
50 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2988 4741 5051 7268 928
Size: 971 1089 1753 310 2217 5421
Fragments arranged by size:
5421 2217 1753 1089 971 310
BbvCI CC'TCA_GC
Cuts at: 6688 6688
Size: 11761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2682 2716 9952 9986 1903
Size: 34 745 34 7236 34 3678
Fragments arranged by size:
7236 3678 745 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2831 3790 8440 9653 10863 2831
Size: 959 4650 1213 1210 3729
Fragments arranged by size:
4650 3729 1213 1210 959
BclI T'GATC_A
Cuts at: 1548 6627 1548
Size: 5079 6682
BfrBI ATG'CAT
Cuts at: 2872 2872
Size: 11761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10372 136
Size: 107 122 71 631 605 8700 1525
Fragments arranged by size:
8700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3868 11756 3868
Size: 7888 3873
BlpI GC'TnA_GC
Cuts at: 9178 9178
Size: 11761
Bme1580I G_kGCm'C
Cuts at: 1790 2090 3217 3379 6523 7993 8010 8320 8449
Size: 300 1127 162 3144 1470 17 310 129
Cuts at: 8449 9025 9822 10978 1790
Size: 576 797 1156 2573
Fragments arranged by size:
3144 2573 1470 1156 1127 797 576 310
300 162 129 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4678 6851 7706
Size: 416 53 83 399 24 3261 2173 855
Cuts at: 7706 9492 10450 442
Size: 1786 958 1753
Fragments arranged by size:
3261 2173 1786 1753 958 855 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2969 3480 5330 1056
Size: 1913 511 1850 7487
Fragments arranged by size:
7487 1913 1850 511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4897 8214 4897
Size: 3317 8444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3054 5178 5967 6576 7671 8151 8862 8925
Size: 1815 2124 789 609 1095 480 711 63
Cuts at: 8925 10421 1239
Size: 1496 2579
Fragments arranged by size:
2579 2124 1815 1496 1095 789 711 609
480 63
Bpu10I CC'TnA_GC
Cuts at: 3223 6381 6688 7074 3223
Size: 3158 307 386 7910
Fragments arranged by size:
7910 3158 386 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3588 3705 5191 5781 9886 10751 11049 3588
Size: 117 1486 590 4105 865 298 4300
Fragments arranged by size:
4300 4105 1486 865 590 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5111 6552 8185 10424 882
Size: 617 3612 1441 1633 2239 2219
Fragments arranged by size:
3612 2239 2219 1633 1441 617
BsaAI yAC'GTr
Cuts at: 493 1204 5297 8552 9552 493
Size: 711 4093 3255 1000 2702
Fragments arranged by size:
4093 3255 2702 1000 711
BsaBI GATnn'nnATC
Cuts at: 7447 11755 7447
Size: 4308 7453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9568 9950 10585 275
Size: 53 83 186 936 8035 382 635 1451
Fragments arranged by size:
8035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2023 2235 3071 6561 10194 10592 10866 11013 11424
Size: 212 836 3490 3633 398 274 147 411
Cuts at: 11424 2023
Size: 2360
Fragments arranged by size:
3633 3490 2360 836 411 398 274 212
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2928 2958 3027 3057 3756
Size: 30 165 30 1525 30 69 30 699
Cuts at: 3756 3786 3954 3984 4402 4432 4742 4772 5833
Size: 30 168 30 418 30 310 30 1061
Cuts at: 5833 5863 7510 7540 7648 7678 8052 8082 8560
Size: 30 1647 30 108 30 374 30 478
Cuts at: 8560 8590 1178
Size: 30 4349
Fragments arranged by size:
4349 1647 1525 1061 699 478 418 374
310 168 165 108 69 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2145 2399 3486 4227 4581 4902 5194 5406
Size: 959 254 1087 741 354 321 292 212
Cuts at: 5406 5648 6495 6676 6900 8343 1186
Size: 242 847 181 224 1443 4604
Fragments arranged by size:
4604 1443 1087 959 847 741 354 321
292 254 242 224 212 181
BseYI C'CCAG_C
Cuts at: 2252 7401 7752 8492 10964 11460 2252
Size: 5149 351 740 2472 496 2553
Fragments arranged by size:
5149 2553 2472 740 496 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2508 2640 4041 4689 5400 7302 8403
Size: 573 772 132 1401 648 711 1902 1101
Cuts at: 8403 8532 1163
Size: 129 4392
Fragments arranged by size:
4392 1902 1401 1101 772 711 648 573
132 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9211 9972 11000 1540
Size: 275 186 7210 761 1028 2301
Fragments arranged by size:
7210 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 3063 4584 4785 5284 6523 8335 8449 9822
Size: 923 1521 201 499 1239 1812 114 1373
Cuts at: 9822 9907 10978 2140
Size: 85 1071 2923
Fragments arranged by size:
2923 1812 1521 1373 1239 1071 923 499
201 114 85
BsmI GAATG_Cn'
Cuts at: 3190 8869 9272 9365 3190
Size: 5679 403 93 5586
Fragments arranged by size:
5679 5586 403 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2157 2157
Size: 11761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2090 2140 3063 3217 3379 4237
Size: 399 273 27 50 923 154 162 858
Cuts at: 4237 4584 4785 4976 5284 6523 7052 7993 8010
Size: 347 201 191 308 1239 529 941 17
Cuts at: 8010 8320 8335 8449 9025 9080 9822 9907 10978
Size: 310 15 114 576 55 742 85 1071
Cuts at: 10978 1391
Size: 2174
Fragments arranged by size:
2174 1239 1071 941 923 858 742 576
529 399 347 310 308 273 201 191
162 154 114 85 55 50 27 17
15
BspEI T'CCGG_A
Cuts at: 11424 11424
Size: 11761
BspHI T'CATG_A
Cuts at: 4498 9650 11380 4498
Size: 5152 1730 4879
Fragments arranged by size:
5152 4879 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 4306 5366 5897 7127 7478 8203 9116
Size: 1346 2116 1060 531 1230 351 725 913
Cuts at: 9116 844
Size: 3489
Fragments arranged by size:
3489 2116 1346 1230 1060 913 725 531
351
BsrBI CCG'CTC
Cuts at: 1394 1823 9648 1394
Size: 429 7825 3507
Fragments arranged by size:
7825 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2874 4365 8503 8754 8874 10256 10430
Size: 1183 1626 1491 4138 251 120 1382 174
Cuts at: 10430 65
Size: 1396
Fragments arranged by size:
4138 1626 1491 1396 1382 1183 251 174
120
BsrFI r'CCGG_y
Cuts at: 1700 1810 10405 10592 1700
Size: 110 8595 187 2869
Fragments arranged by size:
8595 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6320 96
Size: 6224 5537
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11761
BssSI C'ACGA_G
Cuts at: 2157 4239 4535 9821 10833 2157
Size: 2082 296 5286 1012 3085
Fragments arranged by size:
5286 3085 2082 1012 296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2873 794
Size: 2079 9682
BstBI TT'CG_AA
Cuts at: 10565 10565
Size: 11761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11761
BstYI r'GATC_y
Cuts at: 1972 2506 2593 3868 4069 6634 6957 7589 8063
Size: 534 87 1275 201 2565 323 632 474
Cuts at: 8063 8134 9091 9458 9843 9860 11312 11398 11410
Size: 71 957 367 385 17 1452 86 12
Cuts at: 11410 11756 1972
Size: 346 1977
Fragments arranged by size:
2565 1977 1452 1275 957 632 534 474
385 367 346 323 201 87 86 71
17 12
Bsu36I CC'TnA_GG
Cuts at: 2495 4278 8209 2495
Size: 1783 3931 6047
Fragments arranged by size:
6047 3931 1783
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2568 4263 7296 8979 513
Size: 552 939 90 474 1695 3033 1683 3295
Fragments arranged by size:
3295 3033 1695 1683 939 552 474 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6135 487
Size: 683 56 4909 6113
Fragments arranged by size:
6113 4909 683 56
BtsI GCAGTG_nn'
Cuts at: 3309 4044 4256 4553 5010 6726 7120 7314 9277
Size: 735 212 297 457 1716 394 194 1963
Cuts at: 9277 10071 10091 3309
Size: 794 20 4979
Fragments arranged by size:
4979 1963 1716 794 735 457 394 297
212 194 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4596 4631 4698 4733 6954 6989 7443
Size: 35 4032 35 67 35 2221 35 454
Cuts at: 7443 7478 8987 9022 529
Size: 35 1509 35 3268
Fragments arranged by size:
4032 3268 2221 1509 454 67 35 35
35 35 35 35
DraI TTT'AAA
Cuts at: 3205 4227 9065 3205
Size: 1022 4838 5901
Fragments arranged by size:
5901 4838 1022
DraIII CAC_nnn'GTG
Cuts at: 7087 7934 7087
Size: 847 10914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10768 809
Size: 507 9452 1802
Fragments arranged by size:
9452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3337 4126 4531
Size: 54 1425 50 275 18 1507 789 405
Cuts at: 4531 9208 10097 11726 8
Size: 4677 889 1629 43
Fragments arranged by size:
4677 1629 1507 1425 889 789 405 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9208 1537
Size: 275 7396 4090
Fragments arranged by size:
7396 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2376 3480 4323 4342 5705 6231 6472
Size: 1002 21 1104 843 19 1363 526 241
Cuts at: 6472 6897 7623 7903 9691 1353
Size: 425 726 280 1788 3423
Fragments arranged by size:
3423 1788 1363 1104 1002 843 726 526
425 280 241 21 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 2331 10348 10722 10868 387
Size: 1064 138 528 214 8017 374 146 1280
Fragments arranged by size:
8017 1280 1064 528 374 214 146 138
EcoICRI GAG'CTC
Cuts at: 2138 5282 2138
Size: 3144 8617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2619 3054 3839 3906 4425 5067
Size: 393 578 409 435 785 67 519 642
Cuts at: 5067 5178 5564 5622 5853 5967 6576 7604 7671
Size: 111 386 58 231 114 609 1028 67
Cuts at: 7671 7815 8151 8862 8925 9824 10421 11208 11476
Size: 144 336 711 63 899 597 787 268
Cuts at: 11476 1239
Size: 1524
Fragments arranged by size:
1524 1028 899 787 785 711 642 609
597 578 519 435 409 393 386 336
268 231 144 114 111 67 67 63
58
EcoNI CCTnn'n_nnAGG
Cuts at: 3485 3485
Size: 11761
EcoO109I rG'GnC_Cy
Cuts at: 2087 8007 8909 2087
Size: 5920 902 4939
Fragments arranged by size:
5920 4939 902
EcoRI G'AATT_C
Cuts at: 7129 7822 7945 9070 9448 7129
Size: 693 123 1125 378 9442
Fragments arranged by size:
9442 1125 693 378 123
EcoRV GAT'ATC
Cuts at: 6462 6462
Size: 11761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 3079 3918 5828 7242 2482
Size: 597 839 1910 1414 7001
Fragments arranged by size:
7001 1910 1414 839 597
FspI TGC'GCA
Cuts at: 10267 10267
Size: 11761
HaeII r_GCGC'y
Cuts at: 1678 4511 10908 1678
Size: 2833 6397 2531
Fragments arranged by size:
6397 2833 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 2491 2523 2928
Size: 32 135 32 815 32 72 32 405
Cuts at: 2928 2960 3564 3596 4402 4434 4958 4990 5401
Size: 32 604 32 806 32 524 32 411
Cuts at: 5401 5433 5833 5865 6145 6177 6230 6262 6619
Size: 32 400 32 280 32 53 32 357
Cuts at: 6619 6651 7427 7459 7648 7680 8677 8709 10402
Size: 32 776 32 189 32 997 32 1693
Cuts at: 10402 10434 10476 10508 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1693 997 815 806 776 604 524
411 405 400 357 280 189 135 72
53 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2616 4206 6366 8085 8385 9105
Size: 988 281 678 1590 2160 1719 300 720
Cuts at: 9105 9351 669
Size: 246 3079
Fragments arranged by size:
3079 2160 1719 1590 988 720 678 300
281 246
HindIII A'AGCT_T
Cuts at: 748 2532 5810 748
Size: 1784 3278 6699
Fragments arranged by size:
6699 3278 1784
HpaI GTT'AAC
Cuts at: 4206 6366 9351 4206
Size: 2160 2985 6616
Fragments arranged by size:
6616 2985 2160
KpnI G_GTAC'C
Cuts at: 9086 9086
Size: 11761
MfeI C'AATT_G
Cuts at: 6611 9360 6611
Size: 2749 9012
MluI A'CGCG_T
Cuts at: 11637 11637
Size: 11761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 2682 4439 5648 10875 11059 1305
Size: 1314 63 1757 1209 5227 184 2007
Fragments arranged by size:
5227 2007 1757 1314 1209 184 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3339 4128 4533 10
Size: 54 1425 343 1507 789 405 7238
Fragments arranged by size:
7238 1507 1425 789 405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2877 3195 3834 4134 4638 4788 5289
Size: 648 1711 318 639 300 504 150 501
Cuts at: 5289 5349 7256 8799 9719 10078 10237 518
Size: 60 1907 1543 920 359 159 2042
Fragments arranged by size:
2042 1907 1711 1543 920 648 639 504
501 359 318 300 159 150 60
MspA1I CmG'CkG
Cuts at: 1606 2096 2289 3372 4628 4946 6114 6173 8342
Size: 490 193 1083 1256 318 1168 59 2169
Cuts at: 8342 9177 9854 11002 11247 1606
Size: 835 677 1148 245 2120
Fragments arranged by size:
2169 2120 1256 1168 1148 1083 835 677
490 318 245 193 59
NcoI C'CATG_G
Cuts at: 513 1065 2004 4263 513
Size: 552 939 2259 8011
Fragments arranged by size:
8011 2259 939 552
NdeI CA'TA_TG
Cuts at: 387 3022 3811 8236 9045 387
Size: 2635 789 4425 809 3103
Fragments arranged by size:
4425 3103 2635 809 789
NheI G'CTAG_C
Cuts at: 1052 2965 3476 5326 1052
Size: 1913 511 1850 7487
Fragments arranged by size:
7487 1913 1850 511
NotI GC'GGCC_GC
Cuts at: 9208 9208
Size: 11761
NsiI A_TGCA'T
Cuts at: 2874 2874
Size: 11761
NspI r_CATG'y
Cuts at: 1132 1229 2876 2945 3143 3391 3432 3443 6929
Size: 97 1647 69 198 248 41 11 3486
Cuts at: 6929 8804 9119 10664 1132
Size: 1875 315 1545 2229
Fragments arranged by size:
3486 2229 1875 1647 1545 315 248 198
97 69 41 11
PasI CC'CwG_GG
Cuts at: 1348 8681 9018 1348
Size: 7333 337 4091
Fragments arranged by size:
7333 4091 337
PciI A'CATG_T
Cuts at: 2941 3139 10660 2941
Size: 198 7521 4042
Fragments arranged by size:
7521 4042 198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 11761
PfoI T'CCnGG_A
Cuts at: 7585 7585
Size: 11761
PmeI GTTT'AAAC
Cuts at: 9065 9065
Size: 11761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3756 3788 8560 8592 9830 9862 11343 11375 3756
Size: 32 4772 32 1238 32 1481 32 4142
Fragments arranged by size:
4772 4142 1481 1238 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 8909 8909
Size: 11761
PshAI GACnn'nnGTC
Cuts at: 1613 8713 1613
Size: 7100 4661
PsiI TTA'TAA
Cuts at: 9331 9331
Size: 11761
PspOMI G'GGCC_C
Cuts at: 2086 8006 2086
Size: 5920 5841
PspXI vC'TCGA_Gb
Cuts at: 4764 4764
Size: 11761
PstI C_TGCA'G
Cuts at: 830 2047 2551 3763 6119 8812 8938 9113 830
Size: 1217 504 1212 2356 2693 126 175 3478
Fragments arranged by size:
3478 2693 2356 1217 1212 504 175 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11761
PvuII CAG'CTG
Cuts at: 4628 4946 6114 6173 8342 4628
Size: 318 1168 59 2169 8047
Fragments arranged by size:
8047 2169 1168 318 59
SacI G_AGCT'C
Cuts at: 2140 5284 2140
Size: 3144 8617
SacII CC_GC'GG
Cuts at: 2097 2097
Size: 11761
SalI G'TCGA_C
Cuts at: 1936 9103 1936
Size: 7167 4594
SapI GCTCTTCn'nnn_
Cuts at: 4342 4342
Size: 11761
SbfI CC_TGCA'GG
Cuts at: 8812 9113 8812
Size: 301 11460
ScaI AGT'ACT
Cuts at: 2604 10009 11721 2604
Size: 7405 1712 2644
Fragments arranged by size:
7405 2644 1712
SfcI C'TryA_G
Cuts at: 826 1045 2043 2547 3759 6115 6768 8808 8934
Size: 219 998 504 1212 2356 653 2040 126
Cuts at: 8934 9109 10244 10925 11116 826
Size: 175 1135 681 191 1471
Fragments arranged by size:
2356 2040 1471 1212 1135 998 681 653
504 219 191 175 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11761
SmaI CCC'GGG
Cuts at: 1872 9088 1872
Size: 7216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3528 3603 3720 4764 5206 5796
Size: 197 926 1585 75 117 1044 442 590
Cuts at: 5796 7921 9865 10766 11028 820
Size: 2125 1944 901 262 1553
Fragments arranged by size:
2125 1944 1585 1553 1044 926 901 590
442 262 197 117 75
SnaBI TAC'GTA
Cuts at: 493 5297 9552 493
Size: 4804 4255 2702
Fragments arranged by size:
4804 4255 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11761
SphI G_CATG'C
Cuts at: 1132 2876 9119 1132
Size: 1744 6243 3774
Fragments arranged by size:
6243 3774 1744
SspI AAT'ATT
Cuts at: 5 52 4653 4854 9685 5
Size: 47 4601 201 4831 2081
Fragments arranged by size:
4831 4601 2081 201 47
StuI AGG'CCT
Cuts at: 1517 6874 7042 1517
Size: 5357 168 6236
Fragments arranged by size:
6236 5357 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 4263 4698 4716 5035 6968 8323
Size: 552 939 2259 435 18 319 1933 1355
Cuts at: 8323 9200 513
Size: 877 3074
Fragments arranged by size:
3074 2259 1933 1355 939 877 552 435
319 18
SwaI ATTT'AAAT
Cuts at: 4227 4227
Size: 11761
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7336 8725 9788 9805 9958 10143 10588
Size: 1255 5756 1389 1063 17 153 185 445
Cuts at: 10588 325
Size: 1498
Fragments arranged by size:
5756 1498 1389 1255 1063 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2602 3935 4003
Size: 274 80 33 51 1443 625 1333 68
Cuts at: 4003 5575 6320 7925 7951 8762 10007 11719 96
Size: 1572 745 1605 26 811 1245 1712 138
Fragments arranged by size:
1712 1605 1572 1443 1333 1245 811 745
625 274 138 80 68 51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3954 3986 4303 4335 7009 7041 7215 7247 7510
Size: 32 317 32 2674 32 174 32 263
Cuts at: 7510 7542 11721 11753 3954
Size: 32 4179 32 3962
Fragments arranged by size:
4179 3962 2674 317 263 174 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2620 1316
Size: 1304 10457
XbaI T'CTAG_A
Cuts at: 4883 7418 9097 4883
Size: 2535 1679 7547
Fragments arranged by size:
7547 2535 1679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3098 3098
Size: 11761
XhoI C'TCGA_G
Cuts at: 1943 3528 4764 1943
Size: 1585 1236 8940
Fragments arranged by size:
8940 1585 1236
XmaI C'CCGG_G
Cuts at: 1870 9086 1870
Size: 7216 4545
XmnI GAAnn'nnTTC
Cuts at: 2604 5991 6660 9890 2604
Size: 3387 669 3230 4475
Fragments arranged by size:
4475 3387 3230 669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9569 276
Size: 53 83 186 936 8035 2468
Fragments arranged by size:
8035 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PsrI RsrII SanDI SexAI SfiI SfoI SrfI