Restriction map for
pFN21AB7321
(Circular) (Six-base) MAPSORT of: pFN21AB7321.seq Check: 9715 from: 1 to: 8302
pFN21AB7321 8302 bp
With 182 enzymes: SgfI *
January 27, 2015 16:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4435 4435
Size: 8302
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6112 278
Size: 53 83 186 936 4576 2468
Fragments arranged by size:
4576 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 4951 5645 1937
Size: 741 2273 694 4594
Fragments arranged by size:
4594 2273 741 694
Acc65I G'GTAC_C
Cuts at: 2491 5623 2491
Size: 3132 5170
AclI AA'CG_TT
Cuts at: 1563 6429 6802 1563
Size: 4866 373 3063
Fragments arranged by size:
4866 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 3134 4571 6365 7749 8017 1632
Size: 824 678 1437 1794 1384 268 1917
Fragments arranged by size:
1917 1794 1437 1384 824 678 268
AfeI AGC'GCT
Cuts at: 5389 5389
Size: 8302
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8105
AflIII A'CryG_T
Cuts at: 1203 2413 2891 4600 4788 7201 8178 1203
Size: 1210 478 1709 188 2413 977 1327
Fragments arranged by size:
2413 1709 1327 1210 977 478 188
AgeI A'CCGG_T
Cuts at: 7133 7133
Size: 8302
AhdI GACnn_n'nnGTC
Cuts at: 7031 7031
Size: 8302
AleI CACnn'nnGTG
Cuts at: 1166 3478 1166
Size: 2312 5990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8302
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5099 5131 5099
Size: 32 8270
AlwNI CAG_nnn'CTG
Cuts at: 6045 7617 6045
Size: 1572 6730
ApaLI G'TGCA_C
Cuts at: 6359 7515 6359
Size: 1156 7146
ApoI r'AATT_y
Cuts at: 1493 2119 2554 2618 2870 2883 3120 3404 3593
Size: 626 435 64 252 13 237 284 189
Cuts at: 3593 3926 4016 4123 4876 5611 5837 5989 8098
Size: 333 90 107 753 735 226 152 2109
Cuts at: 8098 1493
Size: 1697
Fragments arranged by size:
2109 1697 753 735 626 435 333 284
252 237 226 189 152 107 90 64
13
AseI AT'TA_AT
Cuts at: 160 1032 6856 160
Size: 872 5824 1606
Fragments arranged by size:
5824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8302
AvaI C'yCGr_G
Cuts at: 1870 1943 5627 1870
Size: 73 3684 4545
Fragments arranged by size:
4545 3684 73
BamHI G'GATC_C
Cuts at: 3722 5632 5999 3722
Size: 1910 367 6025
Fragments arranged by size:
6025 1910 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 5623 7078 618
Size: 325 217 627 704 3132 1455 1842
Fragments arranged by size:
3132 1842 1455 704 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 5223 5621 1391
Size: 637 3195 398 4072
Fragments arranged by size:
4072 3195 637 398
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 2917 4262 928
Size: 971 572 446 1345 4968
Fragments arranged by size:
4968 1345 971 572 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6493 6527 1903
Size: 34 50 34 4472 34 3678
Fragments arranged by size:
4472 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5143 6194 7404 5143
Size: 1051 1210 6041
Fragments arranged by size:
6041 1210 1051
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8302
BfrBI ATG'CAT
Cuts at: 2791 5374 2791
Size: 2583 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6913 136
Size: 107 122 71 631 605 5241 1525
Fragments arranged by size:
5241 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8297 8297
Size: 8302
BlpI GC'TnA_GC
Cuts at: 5719 5719
Size: 8302
Bme1580I G_kGCm'C
Cuts at: 1790 6363 7519 1790
Size: 4573 1156 2573
Fragments arranged by size:
4573 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2490 5302 6033
Size: 416 53 83 399 24 1073 2812 731
Cuts at: 6033 6991 442
Size: 958 1753
Fragments arranged by size:
2812 1753 1073 958 731 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5244 1056
Size: 4188 4114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2544 2544
Size: 8302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4011 4918 5199 5539 6962 1239
Size: 2772 907 281 340 1423 2579
Fragments arranged by size:
2772 2579 1423 907 340 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5109 6427 7292 7590 5109
Size: 1318 865 298 5821
Fragments arranged by size:
5821 1318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6965 882
Size: 617 5466 2219
Fragments arranged by size:
5466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3685 6093 493
Size: 711 2481 2408 2702
Fragments arranged by size:
2702 2481 2408 711
BsaBI GATnn'nnATC
Cuts at: 2444 3846 8296 2444
Size: 1402 4450 2450
Fragments arranged by size:
4450 2450 1402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6109 6491 7126 275
Size: 53 83 186 936 4576 382 635 1451
Fragments arranged by size:
4576 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6735 7133 7407 7554 7965 6735
Size: 398 274 147 411 7072
Fragments arranged by size:
7072 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 2891 2921 3822
Size: 30 165 30 1257 30 201 30 901
Cuts at: 3822 3852 1178
Size: 30 5628
Fragments arranged by size:
5628 1257 901 201 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3209 5147 5150 1186
Size: 2023 1938 3 4338
Fragments arranged by size:
4338 2023 1938 3
BseYI C'CCAG_C
Cuts at: 3057 5265 7505 8001 3057
Size: 2208 2240 496 3358
Fragments arranged by size:
3358 2240 2208 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3728 4410 1163
Size: 573 1992 682 5055
Fragments arranged by size:
5055 1992 682 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5752 6513 7541 1540
Size: 275 186 3751 761 1028 2301
Fragments arranged by size:
3751 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2530 4139 5466 6363 6448 7519 2530
Size: 1609 1327 897 85 1071 3313
Fragments arranged by size:
3313 1609 1327 1071 897 85
BsmI GAATG_Cn'
Cuts at: 5813 5906 5813
Size: 93 8209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2530 4139 5223 5466 5621 6363
Size: 399 238 502 1609 1084 243 155 742
Cuts at: 6363 6448 7519 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1609 1084 1071 742 502 399 243
238 155 85
BspEI T'CCGG_A
Cuts at: 7965 7965
Size: 8302
BspHI T'CATG_A
Cuts at: 2683 2857 5158 6191 7921 2683
Size: 174 2301 1033 1730 3064
Fragments arranged by size:
3064 2301 1730 1033 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4435 5562 5657 844
Size: 3591 1127 95 3489
Fragments arranged by size:
3591 3489 1127 95
BsrBI CCG'CTC
Cuts at: 1394 1823 6189 1394
Size: 429 4366 3507
Fragments arranged by size:
4366 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 2828 3864 4278 4733 4846 6797
Size: 1183 1395 185 1036 414 455 113 1951
Cuts at: 6797 6971 65
Size: 174 1396
Fragments arranged by size:
1951 1396 1395 1183 1036 455 414 185
174 113
BsrFI r'CCGG_y
Cuts at: 1700 1810 6946 7133 1700
Size: 110 5136 187 2869
Fragments arranged by size:
5136 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2603 96
Size: 2507 5795
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8302
BssSI C'ACGA_G
Cuts at: 6362 7374 6362
Size: 1012 7290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2348 2642 3863 4118 794
Size: 1554 294 1221 255 4978
Fragments arranged by size:
4978 1554 1221 294 255
BstBI TT'CG_AA
Cuts at: 7106 7106
Size: 8302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8302
BstYI r'GATC_y
Cuts at: 1972 2779 3722 3847 5632 5999 6384 6401 7853
Size: 807 943 125 1785 367 385 17 1452
Cuts at: 7853 7939 7951 8297 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1785 1452 943 807 385 367 346
125 86 17 12
BstZ17I GTA'TAC
Cuts at: 2679 2679
Size: 8302
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7750
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7563
Fragments arranged by size:
7563 683 56
BtsI GCAGTG_nn'
Cuts at: 3572 3737 4076 5818 6612 6632 3572
Size: 165 339 1742 794 20 5242
Fragments arranged by size:
5242 1742 794 339 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8267
DraI TTT'AAA
Cuts at: 4627 5190 5606 4627
Size: 563 416 7323
Fragments arranged by size:
7323 563 416
DraIII CAC_nnn'GTG
Cuts at: 3370 4654 3370
Size: 1284 7018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7309 809
Size: 507 5993 1802
Fragments arranged by size:
5993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2751 4410 4645
Size: 54 1425 50 275 18 921 1659 235
Cuts at: 4645 5234 5749 6638 8267 8
Size: 589 515 889 1629 43
Fragments arranged by size:
1659 1629 1425 921 889 589 515 275
235 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5749 1537
Size: 275 3937 4090
Fragments arranged by size:
4090 3937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4175 6232 1353
Size: 2822 2057 3423
Fragments arranged by size:
3423 2822 2057
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6889 7263 7409 387
Size: 1064 138 5300 374 146 1280
Fragments arranged by size:
5300 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 3134 4011 4571 4918 5199 5539
Size: 393 824 678 877 560 347 281 340
Cuts at: 5539 6365 6962 7749 8017 1239
Size: 826 597 787 268 1524
Fragments arranged by size:
1524 877 826 824 787 678 597 560
393 347 340 281 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2364 4768 2364
Size: 2404 5898
EcoO109I rG'GnC_Cy
Cuts at: 2431 2431
Size: 8302
EcoRI G'AATT_C
Cuts at: 4876 5611 5989 4876
Size: 735 378 7189
Fragments arranged by size:
7189 735 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2495 2903 2941 3383 4186 2495
Size: 408 38 442 803 6611
Fragments arranged by size:
6611 803 442 408 38
FspI TGC'GCA
Cuts at: 6808 6808
Size: 8302
HaeII r_GCGC'y
Cuts at: 1678 5391 7449 1678
Size: 3713 2058 2531
Fragments arranged by size:
3713 2531 2058
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 2576 2608 3647
Size: 32 135 32 645 32 327 32 1039
Cuts at: 3647 3679 5359 5391 6943 6975 7017 7049 1373
Size: 32 1680 32 1552 32 42 32 2626
Fragments arranged by size:
2626 1680 1552 1039 645 327 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4794 5211 5646 5892 669
Size: 988 281 2856 417 435 246 3079
Fragments arranged by size:
3079 2856 988 435 417 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8302
HpaI GTT'AAC
Cuts at: 5892 5892
Size: 8302
KpnI G_GTAC'C
Cuts at: 2495 5627 2495
Size: 3132 5170
MfeI C'AATT_G
Cuts at: 5901 5901
Size: 8302
MluI A'CGCG_T
Cuts at: 8178 8178
Size: 8302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5077 7416 7600 1305
Size: 3772 2339 184 2007
Fragments arranged by size:
3772 2339 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 4412 4647 10
Size: 54 1425 343 921 1659 235 3665
Fragments arranged by size:
3665 1659 1425 921 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2418 3478 4178 5457 5514 6260 6619
Size: 648 1252 1060 700 1279 57 746 359
Cuts at: 6619 6778 518
Size: 159 2042
Fragments arranged by size:
2042 1279 1252 1060 746 700 648 359
159 57
MspA1I CmG'CkG
Cuts at: 1606 2011 2339 5381 5718 6395 7543 7788 1606
Size: 405 328 3042 337 677 1148 245 2120
Fragments arranged by size:
3042 2120 1148 677 405 337 328 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7750
NdeI CA'TA_TG
Cuts at: 387 3217 3477 387
Size: 2830 260 5212
Fragments arranged by size:
5212 2830 260
NheI G'CTAG_C
Cuts at: 1052 5240 1052
Size: 4188 4114
NotI GC'GGCC_GC
Cuts at: 5749 5749
Size: 8302
NsiI A_TGCA'T
Cuts at: 2793 5376 2793
Size: 2583 5719
NspI r_CATG'y
Cuts at: 1132 1229 2791 2895 4604 4792 5197 5660 7205
Size: 97 1562 104 1709 188 405 463 1545
Cuts at: 7205 1132
Size: 2229
Fragments arranged by size:
2229 1709 1562 1545 463 405 188 104
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8302
PciI A'CATG_T
Cuts at: 2891 4600 4788 7201 2891
Size: 1709 188 2413 3992
Fragments arranged by size:
3992 2413 1709 188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3052 4654 4979 1108
Size: 1944 1602 325 4431
Fragments arranged by size:
4431 1944 1602 325
PfoI T'CCnGG_A
Cuts at: 3667 3667
Size: 8302
PmeI GTTT'AAAC
Cuts at: 5606 5606
Size: 8302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6371 6403 7884 7916 6371
Size: 32 1481 32 6757
Fragments arranged by size:
6757 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2431
Size: 8302
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8302
PsiI TTA'TAA
Cuts at: 2262 5872 2262
Size: 3610 4692
PstI C_TGCA'G
Cuts at: 830 2344 4432 5654 830
Size: 1514 2088 1222 3478
Fragments arranged by size:
3478 2088 1514 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8302
PvuII CAG'CTG
Cuts at: 2339 2339
Size: 8302
SalI G'TCGA_C
Cuts at: 1936 5644 1936
Size: 3708 4594
SbfI CC_TGCA'GG
Cuts at: 5654 5654
Size: 8302
ScaI AGT'ACT
Cuts at: 3448 6550 8262 3448
Size: 3102 1712 3488
Fragments arranged by size:
3488 3102 1712
SfcI C'TryA_G
Cuts at: 826 1045 2340 2727 4078 4250 4428 5418 5650
Size: 219 1295 387 1351 172 178 990 232
Cuts at: 5650 6785 7466 7657 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1351 1295 1135 990 681 387 232
219 191 178 172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8302
SmaI CCC'GGG
Cuts at: 1872 5629 1872
Size: 3757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5124 6406 7307 7569 820
Size: 197 926 3181 1282 901 262 1553
Fragments arranged by size:
3181 1553 1282 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6093 493
Size: 5600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8302
SphI G_CATG'C
Cuts at: 1132 5660 1132
Size: 4528 3774
SspI AAT'ATT
Cuts at: 5 52 6226 5
Size: 47 6174 2081
Fragments arranged by size:
6174 2081 47
StuI AGG'CCT
Cuts at: 1517 5171 1517
Size: 3654 4648
StyI C'CwwG_G
Cuts at: 513 1065 2072 2274 2748 3051 5741 513
Size: 552 1007 202 474 303 2690 3074
Fragments arranged by size:
3074 2690 1007 552 474 303 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3097 5100 5308 6329 6346 6499 6684
Size: 1255 1517 2003 208 1021 17 153 185
Cuts at: 6684 7129 325
Size: 445 1498
Fragments arranged by size:
2003 1517 1498 1255 1021 445 208 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2603 3446 4344
Size: 274 80 33 51 1443 626 843 898
Cuts at: 4344 4926 6548 8260 96
Size: 582 1622 1712 138
Fragments arranged by size:
1712 1622 1443 898 843 626 582 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8262 8294 8262
Size: 32 8270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8302
XbaI T'CTAG_A
Cuts at: 3083 5638 3083
Size: 2555 5747
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2403 3065 4006 2403
Size: 662 941 6699
Fragments arranged by size:
6699 941 662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8302
XmaI C'CCGG_G
Cuts at: 1870 5627 1870
Size: 3757 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 2883 4527 6431 2702
Size: 181 1644 1904 4573
Fragments arranged by size:
4573 1904 1644 181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6110 276
Size: 53 83 186 936 4576 2468
Fragments arranged by size:
4576 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI
BsmBI BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI