Restriction map for
pFN21AB7318
(Circular) (Six-base) MAPSORT of: pFN21AB7318.seq Check: 608 from: 1 to: 8248
pFN21AB7318 8248 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4302 4382 4302
Size: 80 8168
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6058 278
Size: 53 83 186 936 4522 2468
Fragments arranged by size:
4522 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5591 1937
Size: 3654 4594
Acc65I G'GTAC_C
Cuts at: 5569 5569
Size: 8248
AclI AA'CG_TT
Cuts at: 1563 2741 5547 6375 6748 1563
Size: 1178 2806 828 373 3063
Fragments arranged by size:
3063 2806 1178 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 2487 3551 4514 4619 5168 5234 5332
Size: 621 234 1064 963 105 549 66 98
Cuts at: 5332 6311 7695 7963 1632
Size: 979 1384 268 1917
Fragments arranged by size:
1917 1384 1064 979 963 621 549 268
234 105 98 66
AfeI AGC'GCT
Cuts at: 3536 3536
Size: 8248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8051
AflIII A'CryG_T
Cuts at: 1203 3735 7147 8124 1203
Size: 2532 3412 977 1327
Fragments arranged by size:
3412 2532 1327 977
AgeI A'CCGG_T
Cuts at: 2766 5529 7079 2766
Size: 2763 1550 3935
Fragments arranged by size:
3935 2763 1550
AhdI GACnn_n'nnGTC
Cuts at: 6977 6977
Size: 8248
AleI CACnn'nnGTG
Cuts at: 1166 4813 1166
Size: 3647 4601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3993 1184
Size: 2809 5439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3243 3275 3243
Size: 32 8216
AlwNI CAG_nnn'CTG
Cuts at: 3672 4099 4390 4729 4774 4900 5071 5991 7563
Size: 427 291 339 45 126 171 920 1572
Cuts at: 7563 3672
Size: 4357
Fragments arranged by size:
4357 1572 920 427 339 291 171 126
45
ApaLI G'TGCA_C
Cuts at: 6305 7461 6305
Size: 1156 7092
ApoI r'AATT_y
Cuts at: 1493 2231 2514 2571 2672 4152 5557 5783 5935
Size: 738 283 57 101 1480 1405 226 152
Cuts at: 5935 8044 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1480 1405 738 283 226 152
101 57
AseI AT'TA_AT
Cuts at: 160 1032 6802 160
Size: 872 5770 1606
Fragments arranged by size:
5770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
AvaI C'yCGr_G
Cuts at: 1870 1943 3483 3614 5131 5243 5484 5573 1870
Size: 73 1540 131 1517 112 241 89 4545
Fragments arranged by size:
4545 1540 1517 241 131 112 89 73
BamHI G'GATC_C
Cuts at: 5578 5945 5578
Size: 367 7881
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 3355 4280 4484 5569
Size: 325 217 627 810 758 925 204 1085
Cuts at: 5569 7024 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1085 925 810 758 627 325
217 204
BanII G_rGCy'C
Cuts at: 1391 2141 3053 3371 3630 5567 1391
Size: 750 912 318 259 1937 4072
Fragments arranged by size:
4072 1937 912 750 318 259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2887 3305 3794 3882 4349 5016 928
Size: 971 988 418 489 88 467 667 4160
Fragments arranged by size:
4160 988 971 667 489 467 418 88
BbvCI CC'TCA_GC
Cuts at: 2679 3131 4197 4361 4780 2679
Size: 452 1066 164 419 6147
Fragments arranged by size:
6147 1066 452 419 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6439 6473 1903
Size: 34 4502 34 3678
Fragments arranged by size:
4502 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6140 7350 6140
Size: 1210 7038
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6859 136
Size: 107 122 71 631 605 5187 1525
Fragments arranged by size:
5187 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4602 8243 4602
Size: 3641 4607
BlpI GC'TnA_GC
Cuts at: 5665 5665
Size: 8248
Bme1580I G_kGCm'C
Cuts at: 1790 3188 3360 6309 7465 1790
Size: 1398 172 2949 1156 2573
Fragments arranged by size:
2949 2573 1398 1156 172
BmgBI CAC'GTC
Cuts at: 1318 3181 3403 5397 1318
Size: 1863 222 1994 4169
Fragments arranged by size:
4169 1994 1863 222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3097 3958 4705
Size: 416 53 83 399 24 1680 861 747
Cuts at: 4705 5979 6937 442
Size: 1274 958 1753
Fragments arranged by size:
1753 1680 1274 958 861 747 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 5305 2971
Size: 2334 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2941 3533 3692 3914 4112 4456 4757 5048
Size: 1702 592 159 222 198 344 301 291
Cuts at: 5048 5093 5480 6908 1239
Size: 45 387 1428 2579
Fragments arranged by size:
2579 1702 1428 592 387 344 301 291
222 198 159 45
Bpu10I CC'TnA_GC
Cuts at: 2135 2679 3131 4197 4361 4780 4985 4991 2135
Size: 544 452 1066 164 419 205 6 5392
Fragments arranged by size:
5392 1066 544 452 419 205 164 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3182 3293 4014 4720 5462 6373 7238 7536 3182
Size: 111 721 706 742 911 865 298 3894
Fragments arranged by size:
3894 911 865 742 721 706 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3958 6911 882
Size: 617 2459 2953 2219
Fragments arranged by size:
2953 2459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2063 2662 3736 6039 493
Size: 711 859 599 1074 2303 2702
Fragments arranged by size:
2702 2303 1074 859 711 599
BsaBI GATnn'nnATC
Cuts at: 8242 8242
Size: 8248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4005 6055 6437 7072
Size: 53 83 186 936 2472 2050 382 635
Cuts at: 7072 275
Size: 1451
Fragments arranged by size:
2472 2050 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2766 2983 4797 5529 6681 7079 7353 7500 7911
Size: 217 1814 732 1152 398 274 147 411
Cuts at: 7911 2766
Size: 3103
Fragments arranged by size:
3103 1814 1152 732 411 398 274 217
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 3548 3578 1178
Size: 30 165 30 1581 30 534 30 5848
Fragments arranged by size:
5848 1581 534 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2327 2696 2699 3692 4262 5149 1186
Size: 1141 369 3 993 570 887 4285
Fragments arranged by size:
4285 1141 993 887 570 369 3
BseYI C'CCAG_C
Cuts at: 2853 3904 4628 7451 7947 2853
Size: 1051 724 2823 496 3154
Fragments arranged by size:
3154 2823 1051 724 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2783 3434 3929 4445 5357 5489 1163
Size: 573 1047 651 495 516 912 132 3922
Fragments arranged by size:
3922 1047 912 651 573 516 495 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5698 6459 7487 1540
Size: 275 186 3697 761 1028 2301
Fragments arranged by size:
3697 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2068 2141 3630 3637 6309 6394 7465 2068
Size: 73 1489 7 2672 85 1071 2851
Fragments arranged by size:
2851 2672 1489 1071 85 73 7
BsmI GAATG_Cn'
Cuts at: 2317 5509 5759 5852 2317
Size: 3192 250 93 4713
Fragments arranged by size:
4713 3192 250 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3171 3171
Size: 8248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2141 3053 3188 3360 3371 3630
Size: 399 278 73 912 135 172 11 259
Cuts at: 3630 3637 5567 6309 6394 7465 1391
Size: 7 1930 742 85 1071 2174
Fragments arranged by size:
2174 1930 1071 912 742 399 278 259
172 135 85 73 11 7
BspEI T'CCGG_A
Cuts at: 7911 7911
Size: 8248
BspHI T'CATG_A
Cuts at: 3321 5040 6137 7867 3321
Size: 1719 1097 1730 3702
Fragments arranged by size:
3702 1730 1719 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2415 4302 4382 5603 844
Size: 1571 1887 80 1221 3489
Fragments arranged by size:
3489 1887 1571 1221 80
BsrBI CCG'CTC
Cuts at: 1394 1823 4333 6135 1394
Size: 429 2510 1802 3507
Fragments arranged by size:
3507 2510 1802 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2207 6743 6917 65
Size: 1183 959 4536 174 1396
Fragments arranged by size:
4536 1396 1183 959 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2766 5310 5529 6892 7079 1700
Size: 110 956 2544 219 1363 187 2869
Fragments arranged by size:
2869 2544 1363 956 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8248
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8248
BssSI C'ACGA_G
Cuts at: 3866 6308 7320 3866
Size: 2442 1012 4794
Fragments arranged by size:
4794 2442 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8248
BstBI TT'CG_AA
Cuts at: 7052 7052
Size: 8248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8248
BstYI r'GATC_y
Cuts at: 1972 2461 4173 4466 4602 5158 5578 5945 6330
Size: 489 1712 293 136 556 420 367 385
Cuts at: 6330 6347 7799 7885 7897 8243 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1712 1452 556 489 420 385 367
346 293 136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3363 3801 4758 513
Size: 552 2298 438 957 4003
Fragments arranged by size:
4003 2298 957 552 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2346 487
Size: 683 56 1120 6389
Fragments arranged by size:
6389 1120 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 2944 4259 5764 6558 6578 2712
Size: 232 1315 1505 794 20 4382
Fragments arranged by size:
4382 1505 1315 794 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3821 3856 4058 4093 529
Size: 35 3257 35 202 35 4684
Fragments arranged by size:
4684 3257 202 35 35 35
DraI TTT'AAA
Cuts at: 5552 5552
Size: 8248
DraIII CAC_nnn'GTG
Cuts at: 2063 2063
Size: 8248
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3796 7255 809
Size: 507 2480 3459 1802
Fragments arranged by size:
3459 2480 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2726 2966 4449
Size: 54 1425 50 275 18 896 240 1483
Cuts at: 4449 5695 6584 8213 8
Size: 1246 889 1629 43
Fragments arranged by size:
1629 1483 1425 1246 896 889 275 240
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5695 1537
Size: 275 3883 4090
Fragments arranged by size:
4090 3883 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4494 5144 6178 1353
Size: 3141 650 1034 3423
Fragments arranged by size:
3423 3141 1034 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6835 7209 7355 387
Size: 1064 138 5246 374 146 1280
Fragments arranged by size:
5246 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2139 3628 2139
Size: 1489 6759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2487 2941 3533 3551 3692 3914
Size: 393 621 234 454 592 18 141 222
Cuts at: 3914 4112 4456 4514 4619 4757 5048 5093 5168
Size: 198 344 58 105 138 291 45 75
Cuts at: 5168 5234 5332 5480 6311 6908 7695 7963 1239
Size: 66 98 148 831 597 787 268 1524
Fragments arranged by size:
1524 831 787 621 597 592 454 393
344 291 268 234 222 198 148 141
138 105 98 75 66 58 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4244 4244
Size: 8248
EcoO109I rG'GnC_Cy
Cuts at: 2087 3139 3745 3898 5068 2087
Size: 1052 606 153 1170 5267
Fragments arranged by size:
5267 1170 1052 606 153
EcoRI G'AATT_C
Cuts at: 5557 5935 5557
Size: 378 7870
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3871 4097 3871
Size: 226 8022
FspI TGC'GCA
Cuts at: 6754 6754
Size: 8248
HaeII r_GCGC'y
Cuts at: 1678 3502 3538 7395 1678
Size: 1824 36 3857 2531
Fragments arranged by size:
3857 2531 1824 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2984 3016 3035 3067 3197
Size: 32 135 32 1412 32 19 32 130
Cuts at: 3197 3229 3477 3509 4253 4285 4894 4926 5102
Size: 32 248 32 744 32 609 32 176
Cuts at: 5102 5134 5143 5175 6889 6921 6963 6995 1373
Size: 32 9 32 1714 32 42 32 2626
Fragments arranged by size:
2626 1714 1412 744 609 248 176 135
130 42 32 32 32 32 32 32
32 32 32 32 32 32 19 9
HincII GTy'rAC
Cuts at: 669 1657 1938 5592 5838 669
Size: 988 281 3654 246 3079
Fragments arranged by size:
3654 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3938 5024 748
Size: 3190 1086 3972
Fragments arranged by size:
3972 3190 1086
HpaI GTT'AAC
Cuts at: 5838 5838
Size: 8248
KpnI G_GTAC'C
Cuts at: 5573 5573
Size: 8248
MfeI C'AATT_G
Cuts at: 5847 5847
Size: 8248
MluI A'CGCG_T
Cuts at: 8124 8124
Size: 8248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4530 4848 7362 7546 1305
Size: 3225 318 2514 184 2007
Fragments arranged by size:
3225 2514 2007 318 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2968 4451 10
Size: 54 1425 343 1136 1483 3807
Fragments arranged by size:
3807 1483 1425 1136 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2634 3380 4813 6206 6565 6724 518
Size: 648 1468 746 1433 1393 359 159 2042
Fragments arranged by size:
2042 1468 1433 1393 746 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2549 2923 3394 4196 4303 5664 6341 7489
Size: 943 374 471 802 107 1361 677 1148
Cuts at: 7489 7734 1606
Size: 245 2120
Fragments arranged by size:
2120 1361 1148 943 802 677 471 374
245 107
NcoI C'CATG_G
Cuts at: 513 1065 3801 513
Size: 552 2736 4960
Fragments arranged by size:
4960 2736 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8248
NotI GC'GGCC_GC
Cuts at: 5695 5695
Size: 8248
NspI r_CATG'y
Cuts at: 1132 1229 2286 4206 5259 5606 7151 1132
Size: 97 1057 1920 1053 347 1545 2229
Fragments arranged by size:
2229 1920 1545 1057 1053 347 97
PasI CC'CwG_GG
Cuts at: 1348 3922 5176 1348
Size: 2574 1254 4420
Fragments arranged by size:
4420 2574 1254
PciI A'CATG_T
Cuts at: 7147 7147
Size: 8248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2964 4099 4774 5071 1108
Size: 1856 1135 675 297 4285
Fragments arranged by size:
4285 1856 1135 675 297
PfoI T'CCnGG_A
Cuts at: 3708 5064 3708
Size: 1356 6892
PmeI GTTT'AAAC
Cuts at: 5552 5552
Size: 8248
PmlI CAC'GTG
Cuts at: 2063 2662 2063
Size: 599 7649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6317 6349 7830 7862 6317
Size: 32 1481 32 6703
Fragments arranged by size:
6703 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 3139 3898 5068 2087
Size: 1052 759 1170 5267
Fragments arranged by size:
5267 1170 1052 759
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8248
PsiI TTA'TAA
Cuts at: 5818 5818
Size: 8248
PspXI vC'TCGA_Gb
Cuts at: 3483 3483
Size: 8248
PstI C_TGCA'G
Cuts at: 830 2554 3393 3399 4725 4896 5600 830
Size: 1724 839 6 1326 171 704 3478
Fragments arranged by size:
3478 1724 1326 839 704 171 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8248
PvuII CAG'CTG
Cuts at: 2549 3394 4303 2549
Size: 845 909 6494
Fragments arranged by size:
6494 909 845
SacI G_AGCT'C
Cuts at: 2141 3630 2141
Size: 1489 6759
SalI G'TCGA_C
Cuts at: 1936 5590 1936
Size: 3654 4594
SapI GCTCTTCn'nnn_
Cuts at: 5144 5144
Size: 8248
SbfI CC_TGCA'GG
Cuts at: 5600 5600
Size: 8248
ScaI AGT'ACT
Cuts at: 3683 6496 8208 3683
Size: 2813 1712 3723
Fragments arranged by size:
3723 2813 1712
SexAI A'CCwGG_T
Cuts at: 5331 5331
Size: 8248
SfcI C'TryA_G
Cuts at: 826 1045 2550 3389 3395 4721 4885 4892 5596
Size: 219 1505 839 6 1326 164 7 704
Cuts at: 5596 6731 7412 7603 826
Size: 1135 681 191 1471
Fragments arranged by size:
1505 1471 1326 1135 839 704 681 219
191 164 7 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8248
SmaI CCC'GGG
Cuts at: 1872 5575 1872
Size: 3703 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3197 3272 3483 4029 4699 5131
Size: 197 926 1254 75 211 546 670 432
Cuts at: 5131 5477 6352 7253 7515 820
Size: 346 875 901 262 1553
Fragments arranged by size:
1553 1254 926 901 875 670 546 432
346 262 211 197 75
SnaBI TAC'GTA
Cuts at: 493 6039 493
Size: 5546 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8248
SphI G_CATG'C
Cuts at: 1132 5606 1132
Size: 4474 3774
SspI AAT'ATT
Cuts at: 5 52 6172 5
Size: 47 6120 2081
Fragments arranged by size:
6120 2081 47
StuI AGG'CCT
Cuts at: 1517 3447 1517
Size: 1930 6318
StyI C'CwwG_G
Cuts at: 513 1065 2485 3801 5292 5687 513
Size: 552 1420 1316 1491 395 3074
Fragments arranged by size:
3074 1491 1420 1316 552 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4298 4302 4502 4551 5168 6275 6292
Size: 1255 2718 4 200 49 617 1107 17
Cuts at: 6292 6445 6630 7075 325
Size: 153 185 445 1498
Fragments arranged by size:
2718 1498 1255 1107 617 445 200 185
153 49 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3681 3729 6494
Size: 274 80 33 51 1443 1704 48 2765
Cuts at: 6494 8206 96
Size: 1712 138
Fragments arranged by size:
2765 1712 1704 1443 274 138 80 51
48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3548 3580 3882 3914 4504 4536 4647 4679 4831
Size: 32 302 32 590 32 111 32 152
Cuts at: 4831 4863 8208 8240 3548
Size: 32 3345 32 3556
Fragments arranged by size:
3556 3345 590 302 152 111 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8248
XbaI T'CTAG_A
Cuts at: 5584 5584
Size: 8248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4831 5329 4831
Size: 498 7750
XhoI C'TCGA_G
Cuts at: 1943 3483 5131 1943
Size: 1540 1648 5060
Fragments arranged by size:
5060 1648 1540
XmaI C'CCGG_G
Cuts at: 1870 5573 1870
Size: 3703 4545
XmnI GAAnn'nnTTC
Cuts at: 2232 3536 3851 5345 6377 2232
Size: 1304 315 1494 1032 4103
Fragments arranged by size:
4103 1494 1304 1032 315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6056 276
Size: 53 83 186 936 4522 2468
Fragments arranged by size:
4522 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI