Restriction map for
pFN21AB7317
(Circular) (Six-base) MAPSORT of: pFN21AB7317.seq Check: 1750 from: 1 to: 7720
pFN21AB7317 7720 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2081 2363 4722 2081
Size: 282 2359 5079
Fragments arranged by size:
5079 2359 282
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5530 278
Size: 53 83 186 936 3994 2468
Fragments arranged by size:
3994 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2117 5063 1937
Size: 180 2946 4594
Fragments arranged by size:
4594 2946 180
Acc65I G'GTAC_C
Cuts at: 4566 4842 5041 4566
Size: 276 199 7245
Fragments arranged by size:
7245 276 199
AclI AA'CG_TT
Cuts at: 1563 5847 6220 1563
Size: 4284 373 3063
Fragments arranged by size:
4284 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3556 5783 7167 7435 1632
Size: 1924 2227 1384 268 1917
Fragments arranged by size:
2227 1924 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7523
AflIII A'CryG_T
Cuts at: 1203 2112 2208 3528 4464 6619 7596 1203
Size: 909 96 1320 936 2155 977 1327
Fragments arranged by size:
2155 1327 1320 977 936 909 96
AgeI A'CCGG_T
Cuts at: 6551 6551
Size: 7720
AhdI GACnn_n'nnGTC
Cuts at: 2965 6449 2965
Size: 3484 4236
AleI CACnn'nnGTG
Cuts at: 1166 2075 2510 1166
Size: 909 435 6376
Fragments arranged by size:
6376 909 435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2351 1184
Size: 1167 6553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 7688
AlwNI CAG_nnn'CTG
Cuts at: 2858 5463 7035 2858
Size: 2605 1572 3543
Fragments arranged by size:
3543 2605 1572
ApaI G_GGCC'C
Cuts at: 3052 4984 5007 3052
Size: 1932 23 5765
Fragments arranged by size:
5765 1932 23
ApaLI G'TGCA_C
Cuts at: 5777 6933 5777
Size: 1156 6564
ApoI r'AATT_y
Cuts at: 1493 2101 5029 5255 5407 7516 1493
Size: 608 2928 226 152 2109 1697
Fragments arranged by size:
2928 2109 1697 608 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6274 160
Size: 872 5242 1606
Fragments arranged by size:
5242 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7720
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2274 3272 3392 3805 3885 4100
Size: 73 73 258 998 120 413 80 215
Cuts at: 4100 5045 1870
Size: 945 4545
Fragments arranged by size:
4545 998 945 413 258 215 120 80
73 73
BamHI G'GATC_C
Cuts at: 3906 5050 5417 3906
Size: 1144 367 6209
Fragments arranged by size:
6209 1144 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2351 2471 2570 2995
Size: 325 217 627 495 69 120 99 425
Cuts at: 2995 3149 3518 3769 3970 4238 4566 4734 4842
Size: 154 369 251 201 268 328 168 108
Cuts at: 4842 5013 5041 6496 618
Size: 171 28 1455 1842
Fragments arranged by size:
1842 1455 627 495 425 369 328 325
268 251 217 201 171 168 154 120
108 99 69 28
BanII G_rGCy'C
Cuts at: 1391 2237 3052 3272 3279 3393 3547 3825 4347
Size: 846 815 220 7 114 154 278 522
Cuts at: 4347 4984 5007 5039 1391
Size: 637 23 32 4072
Fragments arranged by size:
4072 846 815 637 522 278 220 154
114 32 23 7
BbeI G_GCGC'C
Cuts at: 2286 2286
Size: 7720
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3775 928
Size: 971 1876 4873
Fragments arranged by size:
4873 1876 971
BbvCI CC'TCA_GC
Cuts at: 2531 2936 2971 3553 2531
Size: 405 35 582 6698
Fragments arranged by size:
6698 582 405 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2429 2463 4355 4389 4634 4668 5911
Size: 34 492 34 1892 34 245 34 1243
Cuts at: 5911 5945 1903
Size: 34 3678
Fragments arranged by size:
3678 1892 1243 492 245 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2160 4862 5612 6822 2160
Size: 2702 750 1210 3058
Fragments arranged by size:
3058 2702 1210 750
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2579 2825
Size: 107 122 71 631 605 388 519 246
Cuts at: 2825 3870 4004 4427 5011 6331 136
Size: 1045 134 423 584 1320 1525
Fragments arranged by size:
1525 1320 1045 631 605 584 519 423
388 246 134 122 107 71
BglII A'GATC_T
Cuts at: 7715 7715
Size: 7720
BlpI GC'TnA_GC
Cuts at: 5137 5137
Size: 7720
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2618 2772 2878 3052 3121 3165 3521
Size: 564 264 154 106 174 69 44 356
Cuts at: 3521 3993 4739 4984 5007 5781 6937 1790
Size: 472 746 245 23 774 1156 2573
Fragments arranged by size:
2573 1156 774 746 564 472 356 264
245 174 154 106 69 44 23
BmgBI CAC'GTC
Cuts at: 1318 2115 4465 4912 1318
Size: 797 2350 447 4126
Fragments arranged by size:
4126 2350 797 447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 3820 5451
Size: 416 53 83 399 24 726 1677 1631
Cuts at: 5451 6409 442
Size: 958 1753
Fragments arranged by size:
1753 1677 1631 958 726 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7720
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4177 4177
Size: 7720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2892 2998 3062 3437 3954 4379 6380
Size: 1166 487 106 64 375 517 425 2001
Cuts at: 6380 1239
Size: 2579
Fragments arranged by size:
2579 2001 1166 517 487 425 375 106
64
Bpu10I CC'TnA_GC
Cuts at: 2531 2936 2971 3266 3553 4049 2531
Size: 405 35 295 287 496 6202
Fragments arranged by size:
6202 496 405 295 287 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 3458 3494 5845 6710 7008 2480
Size: 978 36 2351 865 298 3192
Fragments arranged by size:
3192 2351 978 865 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 3587 4275 4842 6383 882
Size: 617 858 1230 688 567 1541 2219
Fragments arranged by size:
2219 1541 1230 858 688 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 2148 2211 3529 5511 493
Size: 711 944 63 1318 1982 2702
Fragments arranged by size:
2702 1982 1318 944 711 63
BsaBI GATnn'nnATC
Cuts at: 3911 4406 7714 3911
Size: 495 3308 3917
Fragments arranged by size:
3917 3308 495
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 2491 4058 5527
Size: 53 83 186 936 750 208 1567 1469
Cuts at: 5527 5909 6544 275
Size: 382 635 1451
Fragments arranged by size:
1567 1469 1451 936 750 635 382 208
186 83 53
BsaWI w'CCGG_w
Cuts at: 4755 6153 6551 6825 6972 7383 4755
Size: 1398 398 274 147 411 5092
Fragments arranged by size:
5092 1398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2744 2774 2882 2912 2996
Size: 30 165 30 1341 30 108 30 84
Cuts at: 2996 3026 4550 4580 1178
Size: 30 1524 30 4318
Fragments arranged by size:
4318 1524 1341 165 108 84 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 2819 3755 3869 4245 4441 4574 4864
Size: 1546 87 936 114 376 196 133 290
Cuts at: 4864 1186
Size: 4042
Fragments arranged by size:
4042 1546 936 376 290 196 133 114
87
BseYI C'CCAG_C
Cuts at: 2265 2347 2836 3165 4983 6923 7419 2265
Size: 82 489 329 1818 1940 496 2566
Fragments arranged by size:
2566 1940 1818 496 489 329 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2809 2962 2970 3203 4589 1163
Size: 573 1073 153 8 233 1386 4294
Fragments arranged by size:
4294 1386 1073 573 233 153 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 2454 5170 5931 6959 1540
Size: 275 186 54 399 2716 761 1028 2301
Fragments arranged by size:
2716 2301 1028 761 399 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 5781 5866 6937 5781
Size: 85 1071 6564
Fragments arranged by size:
6564 1071 85
BsmI GAATG_Cn'
Cuts at: 4965 5231 5324 4965
Size: 266 93 7361
Fragments arranged by size:
7361 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2271 2498 3376 3505 2271
Size: 227 878 129 6486
Fragments arranged by size:
6486 878 227 129
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2354 2618 2772 2878 3052 3121
Size: 399 447 117 264 154 106 174 69
Cuts at: 3121 3165 3272 3279 3393 3521 3547 3825 3993
Size: 44 107 7 114 128 26 278 168
Cuts at: 3993 4347 4739 4984 5007 5039 5781 5866 6937
Size: 354 392 245 23 32 742 85 1071
Cuts at: 6937 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 447 399 392 354 278
264 245 174 168 154 128 117 114
107 106 85 69 44 32 26 23
7
BspEI T'CCGG_A
Cuts at: 4755 7383 4755
Size: 2628 5092
BspHI T'CATG_A
Cuts at: 2229 5609 7339 2229
Size: 3380 1730 2610
Fragments arranged by size:
3380 2610 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2081 2363 3216 4722 5075 844
Size: 1237 282 853 1506 353 3489
Fragments arranged by size:
3489 1506 1237 853 353 282
BsrBI CCG'CTC
Cuts at: 1394 1823 2722 2809 3147 3352 4513 5607 1394
Size: 429 899 87 338 205 1161 1094 3507
Fragments arranged by size:
3507 1161 1094 899 429 338 205 87
BsrDI GCAATG_nn'
Cuts at: 65 1248 2445 4137 6215 6389 65
Size: 1183 1197 1692 2078 174 1396
Fragments arranged by size:
2078 1692 1396 1197 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2795 4016 4241 4839 5010 6364 6551
Size: 110 985 1221 225 598 171 1354 187
Cuts at: 6551 1700
Size: 2869
Fragments arranged by size:
2869 1354 1221 985 598 225 187 171
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7720
BssHII G'CGCG_C
Cuts at: 1925 2087 4915 1925
Size: 162 2828 4730
Fragments arranged by size:
4730 2828 162
BssSI C'ACGA_G
Cuts at: 2260 2765 5780 6792 2260
Size: 505 3015 1012 3188
Fragments arranged by size:
3188 3015 1012 505
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7720
BstBI TT'CG_AA
Cuts at: 6524 6524
Size: 7720
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7720
BstXI CCAn_nnnn'nTGG
Cuts at: 3196 3211 3589 4834 3196
Size: 15 378 1245 6082
Fragments arranged by size:
6082 1245 378 15
BstYI r'GATC_y
Cuts at: 1972 3639 3906 4401 4953 5050 5417 5802 5819
Size: 1667 267 495 552 97 367 385 17
Cuts at: 5819 7271 7357 7369 7715 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1667 1452 552 495 385 367 346
267 97 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4178 4178
Size: 7720
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2082 2125 2622 3582 3993
Size: 552 939 51 27 43 497 960 411
Cuts at: 3993 4117 4389 4584 4806 513
Size: 124 272 195 222 3427
Fragments arranged by size:
3427 960 939 552 497 411 272 222
195 124 51 43 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4417 487
Size: 683 56 3191 3790
Fragments arranged by size:
3790 3191 683 56
BtsI GCAGTG_nn'
Cuts at: 2524 2661 2797 3082 5236 6030 6050 2524
Size: 137 136 285 2154 794 20 4194
Fragments arranged by size:
4194 2154 794 285 137 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7720
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7685
DraI TTT'AAA
Cuts at: 5024 5024
Size: 7720
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6727 809
Size: 507 5411 1802
Fragments arranged by size:
5411 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2067 2451
Size: 54 1425 50 275 18 222 15 384
Cuts at: 2451 2580 2984 3070 3201 3565 3996 4120 4287
Size: 129 404 86 131 364 431 124 167
Cuts at: 4287 4455 5167 6056 7685 8
Size: 168 712 889 1629 43
Fragments arranged by size:
1629 1425 889 712 431 404 384 364
275 222 168 167 131 129 124 86
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2052 2451 5167 1537
Size: 275 240 399 2716 4090
Fragments arranged by size:
4090 2716 399 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 3692 5650 1353
Size: 2339 1958 3423
Fragments arranged by size:
3423 2339 1958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4085 4881 6307 6681 6827 387
Size: 1064 138 2496 796 1426 374 146 1280
Fragments arranged by size:
2496 1426 1280 1064 796 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2892 2998 3062 3437 3556 3954
Size: 393 773 487 106 64 375 119 398
Cuts at: 3954 4379 5783 6380 7167 7435 1239
Size: 425 1404 597 787 268 1524
Fragments arranged by size:
1524 1404 787 773 597 487 425 398
393 375 268 119 106 64
EcoNI CCTnn'n_nnAGG
Cuts at: 3182 4052 4177 3182
Size: 870 125 6725
Fragments arranged by size:
6725 870 125
EcoO109I rG'GnC_Cy
Cuts at: 2269 2525 3049 4006 4182 4798 5003 5004 2269
Size: 256 524 957 176 616 205 1 4985
Fragments arranged by size:
4985 957 616 524 256 205 176 1
EcoRI G'AATT_C
Cuts at: 5029 5407 5029
Size: 378 7342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2560 2383
Size: 177 7543
FspI TGC'GCA
Cuts at: 2258 2516 2630 6226 2258
Size: 258 114 3596 3752
Fragments arranged by size:
3752 3596 258 114
HaeII r_GCGC'y
Cuts at: 1678 2286 6867 1678
Size: 608 4581 2531
Fragments arranged by size:
4581 2531 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2660 2692 2951 2983 3483
Size: 32 135 32 1088 32 259 32 500
Cuts at: 3483 3515 3739 3771 6361 6393 6435 6467 1373
Size: 32 224 32 2590 32 42 32 2626
Fragments arranged by size:
2626 2590 1088 500 259 224 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2118 3298 4309 5064 5310 669
Size: 988 281 180 1180 1011 755 246 3079
Fragments arranged by size:
3079 1180 1011 988 755 281 246 180
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7720
HpaI GTT'AAC
Cuts at: 5310 5310
Size: 7720
KasI G'GCGC_C
Cuts at: 2282 2282
Size: 7720
KpnI G_GTAC'C
Cuts at: 4570 4846 5045 4570
Size: 276 199 7245
Fragments arranged by size:
7245 276 199
MfeI C'AATT_G
Cuts at: 5319 5319
Size: 7720
MluI A'CGCG_T
Cuts at: 7596 7596
Size: 7720
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2370 3285 6834 7018 1305
Size: 1065 915 3549 184 2007
Fragments arranged by size:
3549 2007 1065 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 3203 3998 10
Size: 54 1425 343 750 621 795 3732
Fragments arranged by size:
3732 1425 795 750 621 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2510 3587 4205 4832 4934 5678
Size: 648 909 435 1077 618 627 102 744
Cuts at: 5678 6037 6196 518
Size: 359 159 2042
Fragments arranged by size:
2042 1077 909 744 648 627 618 435
359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2057 2084 2869 2880 3108 3129 3290 3982
Size: 451 27 785 11 228 21 161 692
Cuts at: 3982 4213 4413 4648 4785 4987 5136 5813 6961
Size: 231 200 235 137 202 149 677 1148
Cuts at: 6961 7206 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 785 692 677 451 245 235
231 228 202 200 161 149 137 27
21 11
NaeI GCC'GGC
Cuts at: 2797 4018 4243 5012 2797
Size: 1221 225 769 5505
Fragments arranged by size:
5505 1221 769 225
NarI GG'CG_CC
Cuts at: 2283 2283
Size: 7720
NcoI C'CATG_G
Cuts at: 513 1065 2004 2622 3582 4584 513
Size: 552 939 618 960 1002 3649
Fragments arranged by size:
3649 1002 960 939 618 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7720
NgoMIV G'CCGG_C
Cuts at: 2795 4016 4241 5010 2795
Size: 1221 225 769 5505
Fragments arranged by size:
5505 1221 769 225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7720
NotI GC'GGCC_GC
Cuts at: 5167 5167
Size: 7720
NspI r_CATG'y
Cuts at: 1132 1229 2338 3141 4225 4249 5078 6623 1132
Size: 97 1109 803 1084 24 829 1545 2229
Fragments arranged by size:
2229 1545 1109 1084 829 803 97 24
PasI CC'CwG_GG
Cuts at: 1348 2858 3114 4921 4999 1348
Size: 1510 256 1807 78 4069
Fragments arranged by size:
4069 1807 1510 256 78
PciI A'CATG_T
Cuts at: 6619 6619
Size: 7720
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3334 3583 3636 4413 1108
Size: 2226 249 53 777 4415
Fragments arranged by size:
4415 2226 777 249 53
PfoI T'CCnGG_A
Cuts at: 2559 3414 3726 2559
Size: 855 312 6553
Fragments arranged by size:
6553 855 312
PmeI GTTT'AAAC
Cuts at: 5024 5024
Size: 7720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 3812 3844 5789 5821 7302 7334 2996
Size: 32 784 32 1945 32 1481 32 3382
Fragments arranged by size:
3382 1945 1481 784 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2525 4182 2269
Size: 256 1657 5807
Fragments arranged by size:
5807 1657 256
PshAI GACnn'nnGTC
Cuts at: 1613 3368 3632 1613
Size: 1755 264 5701
Fragments arranged by size:
5701 1755 264
PsiI TTA'TAA
Cuts at: 5290 5290
Size: 7720
PspOMI G'GGCC_C
Cuts at: 3048 4980 5003 3048
Size: 1932 23 5765
Fragments arranged by size:
5765 1932 23
PspXI vC'TCGA_Gb
Cuts at: 4100 4100
Size: 7720
PstI C_TGCA'G
Cuts at: 830 2747 5072 830
Size: 1917 2325 3478
Fragments arranged by size:
3478 2325 1917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7720
PvuII CAG'CTG
Cuts at: 2869 4213 4987 2869
Size: 1344 774 5602
Fragments arranged by size:
5602 1344 774
RsrII CG'GwC_CG
Cuts at: 2392 2392
Size: 7720
SacII CC_GC'GG
Cuts at: 2058 2085 2058
Size: 27 7693
SalI G'TCGA_C
Cuts at: 1936 2116 5062 1936
Size: 180 2946 4594
Fragments arranged by size:
4594 2946 180
SanDI GG'GwC_CC
Cuts at: 2269 2525 2269
Size: 256 7464
SbfI CC_TGCA'GG
Cuts at: 5072 5072
Size: 7720
ScaI AGT'ACT
Cuts at: 3434 5968 7680 3434
Size: 2534 1712 3474
Fragments arranged by size:
3474 2534 1712
SexAI A'CCwGG_T
Cuts at: 2214 4317 2214
Size: 2103 5617
SfcI C'TryA_G
Cuts at: 826 1045 2743 3712 5068 6203 6884 7075 826
Size: 219 1698 969 1356 1135 681 191 1471
Fragments arranged by size:
1698 1471 1356 1135 969 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3870 4004 4427 3870
Size: 134 423 7163
Fragments arranged by size:
7163 423 134
SfoI GGC'GCC
Cuts at: 2284 2284
Size: 7720
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7720
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7720
SmaI CCC'GGG
Cuts at: 1872 2018 3807 5047 1872
Size: 146 1789 1240 4545
Fragments arranged by size:
4545 1789 1240 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 3473 3509 4100 5824 6725
Size: 197 926 552 978 36 591 1724 901
Cuts at: 6725 6987 820
Size: 262 1553
Fragments arranged by size:
1724 1553 978 926 901 591 552 262
197 36
SnaBI TAC'GTA
Cuts at: 493 5511 493
Size: 5018 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7720
SphI G_CATG'C
Cuts at: 1132 2338 4225 4249 5078 1132
Size: 1206 1887 24 829 3774
Fragments arranged by size:
3774 1887 1206 829 24
SspI AAT'ATT
Cuts at: 5 52 5644 5
Size: 47 5592 2081
Fragments arranged by size:
5592 2081 47
StuI AGG'CCT
Cuts at: 1517 3722 3899 1517
Size: 2205 177 5338
Fragments arranged by size:
5338 2205 177
StyI C'CwwG_G
Cuts at: 513 1065 2004 2479 2622 3582 4584 5159 513
Size: 552 939 475 143 960 1002 575 3074
Fragments arranged by size:
3074 1002 960 939 575 552 475 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2151 2746 4226 4597 5012 5747 5764
Size: 1255 571 595 1480 371 415 735 17
Cuts at: 5764 5917 6102 6547 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1480 1255 735 595 571 445 415
371 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 3432 4146
Size: 274 80 33 51 1443 762 693 714
Cuts at: 4146 5966 7678 96
Size: 1820 1712 138
Fragments arranged by size:
1820 1712 1443 762 714 693 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 2855 2882 2887 2914 3768 3800 7680
Size: 32 79 27 5 27 854 32 3880
Cuts at: 7680 7712 2744
Size: 32 2752
Fragments arranged by size:
3880 2752 854 79 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7720
XbaI T'CTAG_A
Cuts at: 5056 5056
Size: 7720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 3196 4210 2179
Size: 1017 1014 5689
Fragments arranged by size:
5689 1017 1014
XhoI C'TCGA_G
Cuts at: 1943 4100 1943
Size: 2157 5563
XmaI C'CCGG_G
Cuts at: 1870 2016 3805 5045 1870
Size: 146 1789 1240 4545
Fragments arranged by size:
4545 1789 1240 146
XmnI GAAnn'nnTTC
Cuts at: 5849 5849
Size: 7720
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5528 276
Size: 53 83 186 936 3994 2468
Fragments arranged by size:
3994 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI
PsrI SacI SapI SrfI SwaI