Restriction map for
pFN21AB7316
(Circular) (Six-base) MAPSORT of: pFN21AB7316.seq Check: 9658 from: 1 to: 7996
pFN21AB7316 7996 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5806 278
Size: 53 83 186 936 4270 2468
Fragments arranged by size:
4270 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5339 1937
Size: 3402 4594
Acc65I G'GTAC_C
Cuts at: 5317 5317
Size: 7996
AclI AA'CG_TT
Cuts at: 1563 2397 6123 6496 1563
Size: 834 3726 373 3063
Fragments arranged by size:
3726 3063 834 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3180 4824 6059 7443 7711 1632
Size: 1548 1644 1235 1384 268 1917
Fragments arranged by size:
1917 1644 1548 1384 1235 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7799
AflIII A'CryG_T
Cuts at: 1203 6895 7872 1203
Size: 5692 977 1327
Fragments arranged by size:
5692 1327 977
AgeI A'CCGG_T
Cuts at: 6827 6827
Size: 7996
AhdI GACnn_n'nnGTC
Cuts at: 6725 6725
Size: 7996
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7996
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3520 3552 3520
Size: 32 7964
AlwNI CAG_nnn'CTG
Cuts at: 2089 4544 4793 4826 5739 7311 2089
Size: 2455 249 33 913 1572 2774
Fragments arranged by size:
2774 2455 1572 913 249 33
ApaI G_GGCC'C
Cuts at: 3095 4466 3095
Size: 1371 6625
ApaLI G'TGCA_C
Cuts at: 6053 7209 6053
Size: 1156 6840
ApoI r'AATT_y
Cuts at: 1493 2077 2301 2341 2375 2431 3469 3655 3835
Size: 584 224 40 34 56 1038 186 180
Cuts at: 3835 3879 4189 4498 5116 5305 5531 5683 7792
Size: 44 310 309 618 189 226 152 2109
Cuts at: 7792 1493
Size: 1697
Fragments arranged by size:
2109 1697 1038 618 584 310 309 226
224 189 186 180 152 56 44 40
34
AseI AT'TA_AT
Cuts at: 160 1032 3336 3390 3687 4511 5031 5193 6550
Size: 872 2304 54 297 824 520 162 1357
Cuts at: 6550 160
Size: 1606
Fragments arranged by size:
2304 1606 1357 872 824 520 297 162
54
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7996
AvaI C'yCGr_G
Cuts at: 1870 1943 5321 1870
Size: 73 3378 4545
Fragments arranged by size:
4545 3378 73
BamHI G'GATC_C
Cuts at: 5326 5693 5326
Size: 367 7629
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5317 6772 618
Size: 325 217 627 3530 1455 1842
Fragments arranged by size:
3530 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2152 2556 2723 3095 3848 4466 5315 1391
Size: 761 404 167 372 753 618 849 4072
Fragments arranged by size:
4072 849 761 753 618 404 372 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4245 4391 5235 928
Size: 971 2346 146 844 3689
Fragments arranged by size:
3689 2346 971 844 146
BbvCI CC'TCA_GC
Cuts at: 3774 3774
Size: 7996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6187 6221 1903
Size: 34 4250 34 3678
Fragments arranged by size:
4250 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 4198 4580 5888 7098 2736
Size: 1462 382 1308 1210 3634
Fragments arranged by size:
3634 1462 1308 1210 382
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6607 136
Size: 107 122 71 631 605 4935 1525
Fragments arranged by size:
4935 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2424 7991 2424
Size: 5567 2429
BlpI GC'TnA_GC
Cuts at: 4794 5413 4794
Size: 619 7377
Bme1580I G_kGCm'C
Cuts at: 1790 3095 4466 6057 7213 1790
Size: 1305 1371 1591 1156 2573
Fragments arranged by size:
2573 1591 1371 1305 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4526 4781 5727
Size: 416 53 83 399 24 3109 255 946
Cuts at: 5727 6685 442
Size: 958 1753
Fragments arranged by size:
3109 1753 958 946 416 399 255 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7996
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5185 5185
Size: 7996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 2632 2737 4045 4923 5112 6656 1239
Size: 1164 229 105 1308 878 189 1544 2579
Fragments arranged by size:
2579 1544 1308 1164 878 229 189 105
Bpu10I CC'TnA_GC
Cuts at: 3774 3774
Size: 7996
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2757 4938 6121 6986 7284 2757
Size: 2181 1183 865 298 3469
Fragments arranged by size:
3469 2181 1183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 6659 882
Size: 617 1203 3957 2219
Fragments arranged by size:
3957 2219 1203 617
BsaAI yAC'GTr
Cuts at: 493 1204 2020 2537 3098 5787 493
Size: 711 816 517 561 2689 2702
Fragments arranged by size:
2702 2689 816 711 561 517
BsaBI GATnn'nnATC
Cuts at: 3262 7990 3262
Size: 4728 3268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5803 6185 6820 275
Size: 53 83 186 936 4270 382 635 1451
Fragments arranged by size:
4270 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6429 6827 7101 7248 7659 6429
Size: 398 274 147 411 6766
Fragments arranged by size:
6766 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7771
Fragments arranged by size:
7771 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 3000 3926 5265 1186
Size: 956 858 926 1339 3917
Fragments arranged by size:
3917 1339 956 926 858
BseYI C'CCAG_C
Cuts at: 3250 4885 7199 7695 3250
Size: 1635 2314 496 3551
Fragments arranged by size:
3551 2314 1635 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4896 1163
Size: 573 3160 4263
Fragments arranged by size:
4263 3160 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4004 5446 6207 7235 1540
Size: 275 186 2003 1442 761 1028 2301
Fragments arranged by size:
2301 2003 1442 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2556 4687 6057 6142 7213 2556
Size: 2131 1370 85 1071 3339
Fragments arranged by size:
3339 2131 1370 1071 85
BsmI GAATG_Cn'
Cuts at: 3241 5507 5600 3241
Size: 2266 93 5637
Fragments arranged by size:
5637 2266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4002 4002
Size: 7996
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2556 2723 3095 3848 4466 4687
Size: 399 362 404 167 372 753 618 221
Cuts at: 4687 5315 6057 6142 7213 1391
Size: 628 742 85 1071 2174
Fragments arranged by size:
2174 1071 753 742 628 618 404 399
372 362 221 167 85
BspEI T'CCGG_A
Cuts at: 7659 7659
Size: 7996
BspHI T'CATG_A
Cuts at: 2155 5885 7615 2155
Size: 3730 1730 2536
Fragments arranged by size:
3730 2536 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5351 844
Size: 4507 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2505 5883 1394
Size: 429 682 3378 3507
Fragments arranged by size:
3507 3378 682 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6491 6665 65
Size: 1183 5243 174 1396
Fragments arranged by size:
5243 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6640 6827 1700
Size: 110 4830 187 2869
Fragments arranged by size:
4830 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7996
BssSI C'ACGA_G
Cuts at: 6056 7068 6056
Size: 1012 6984
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7996
BstBI TT'CG_AA
Cuts at: 6800 6800
Size: 7996
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7996
BstXI CCAn_nnnn'nTGG
Cuts at: 2882 2882
Size: 7996
BstYI r'GATC_y
Cuts at: 1972 2424 5141 5326 5693 6078 6095 7547 7633
Size: 452 2717 185 367 385 17 1452 86
Cuts at: 7633 7645 7991 1972
Size: 12 346 1977
Fragments arranged by size:
2717 1977 1452 452 385 367 346 185
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5094 5094
Size: 7996
BtgI C'CryG_G
Cuts at: 513 1065 2227 513
Size: 552 1162 6282
Fragments arranged by size:
6282 1162 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7257
Fragments arranged by size:
7257 683 56
BtsI GCAGTG_nn'
Cuts at: 4545 4687 5512 6306 6326 4545
Size: 142 825 794 20 6215
Fragments arranged by size:
6215 825 794 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7961
DraI TTT'AAA
Cuts at: 3042 5300 3042
Size: 2258 5738
DraIII CAC_nnn'GTG
Cuts at: 2321 2321
Size: 7996
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7003 809
Size: 507 5687 1802
Fragments arranged by size:
5687 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2851 5443 6332
Size: 54 1425 50 275 18 1021 2592 889
Cuts at: 6332 7961 8
Size: 1629 43
Fragments arranged by size:
2592 1629 1425 1021 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5443 1537
Size: 275 3631 4090
Fragments arranged by size:
4090 3631 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2697 4466 5055 5926 1353
Size: 1344 1769 589 871 3423
Fragments arranged by size:
3423 1769 1344 871 589
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6583 6957 7103 387
Size: 1064 138 4994 374 146 1280
Fragments arranged by size:
4994 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2554 2554
Size: 7996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2632 2737 3180 4045 4824 4923
Size: 393 771 229 105 443 865 779 99
Cuts at: 4923 5112 6059 6656 7443 7711 1239
Size: 189 947 597 787 268 1524
Fragments arranged by size:
1524 947 865 787 779 771 597 443
393 268 229 189 105 99
EcoNI CCTnn'n_nnAGG
Cuts at: 4992 4992
Size: 7996
EcoO109I rG'GnC_Cy
Cuts at: 2262 3617 2262
Size: 1355 6641
EcoRI G'AATT_C
Cuts at: 5305 5683 5305
Size: 378 7618
EcoRV GAT'ATC
Cuts at: 2996 4130 2996
Size: 1134 6862
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2618 2804 3129 2618
Size: 186 325 7485
Fragments arranged by size:
7485 325 186
FspI TGC'GCA
Cuts at: 6502 6502
Size: 7996
HaeII r_GCGC'y
Cuts at: 1678 7143 1678
Size: 5465 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4922 4954 5216 5248 6637
Size: 32 135 32 3350 32 262 32 1389
Cuts at: 6637 6669 6711 6743 1373
Size: 32 42 32 2626
Fragments arranged by size:
3350 2626 1389 262 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5340 5586 669
Size: 988 281 3402 246 3079
Fragments arranged by size:
3402 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7996
HpaI GTT'AAC
Cuts at: 5586 5586
Size: 7996
KpnI G_GTAC'C
Cuts at: 5321 5321
Size: 7996
MfeI C'AATT_G
Cuts at: 5595 5595
Size: 7996
MluI A'CGCG_T
Cuts at: 7872 7872
Size: 7996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 3041 3984 4534 7110 7294 1305
Size: 957 779 943 550 2576 184 2007
Fragments arranged by size:
2576 2007 957 943 779 550 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2853 10
Size: 54 1425 343 1021 5153
Fragments arranged by size:
5153 1425 1021 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2880 4247 4532 5954 6313 6472 518
Size: 648 1714 1367 285 1422 359 159 2042
Fragments arranged by size:
2042 1714 1422 1367 648 359 285 159
MspA1I CmG'CkG
Cuts at: 1606 3250 3347 3442 5412 6089 7237 7482 1606
Size: 1644 97 95 1970 677 1148 245 2120
Fragments arranged by size:
2120 1970 1644 1148 677 245 97 95
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7444
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7996
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7996
NotI GC'GGCC_GC
Cuts at: 5443 5443
Size: 7996
NspI r_CATG'y
Cuts at: 1132 1229 5354 6899 1132
Size: 97 4125 1545 2229
Fragments arranged by size:
4125 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7996
PciI A'CATG_T
Cuts at: 6895 6895
Size: 7996
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7996
PfoI T'CCnGG_A
Cuts at: 2039 3067 2039
Size: 1028 6968
PmeI GTTT'AAAC
Cuts at: 5300 5300
Size: 7996
PmlI CAC'GTG
Cuts at: 3098 3098
Size: 7996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6065 6097 7578 7610 6065
Size: 32 1481 32 6451
Fragments arranged by size:
6451 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2262 2262
Size: 7996
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7996
PsiI TTA'TAA
Cuts at: 5566 5566
Size: 7996
PspOMI G'GGCC_C
Cuts at: 3091 4462 3091
Size: 1371 6625
PstI C_TGCA'G
Cuts at: 830 4216 5059 5348 830
Size: 3386 843 289 3478
Fragments arranged by size:
3478 3386 843 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7996
PvuII CAG'CTG
Cuts at: 3347 3347
Size: 7996
SacI G_AGCT'C
Cuts at: 2556 2556
Size: 7996
SalI G'TCGA_C
Cuts at: 1936 5338 1936
Size: 3402 4594
SbfI CC_TGCA'GG
Cuts at: 5348 5348
Size: 7996
ScaI AGT'ACT
Cuts at: 6244 7956 6244
Size: 1712 6284
SexAI A'CCwGG_T
Cuts at: 4081 4428 4081
Size: 347 7649
SfcI C'TryA_G
Cuts at: 826 1045 4212 4989 5055 5344 6479 7160 7351
Size: 219 3167 777 66 289 1135 681 191
Cuts at: 7351 826
Size: 1471
Fragments arranged by size:
3167 1471 1135 777 681 289 219 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7996
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7996
SmaI CCC'GGG
Cuts at: 1872 5323 1872
Size: 3451 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2772 4953 6100 7001 7263 820
Size: 197 926 829 2181 1147 901 262 1553
Fragments arranged by size:
2181 1553 1147 926 901 829 262 197
SnaBI TAC'GTA
Cuts at: 493 2020 2537 5787 493
Size: 1527 517 3250 2702
Fragments arranged by size:
3250 2702 1527 517
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7996
SphI G_CATG'C
Cuts at: 1132 5354 1132
Size: 4222 3774
SspI AAT'ATT
Cuts at: 5 52 2914 4366 5920 5
Size: 47 2862 1452 1554 2081
Fragments arranged by size:
2862 2081 1554 1452 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7996
StyI C'CwwG_G
Cuts at: 513 1065 2634 5435 513
Size: 552 1569 2801 3074
Fragments arranged by size:
3074 2801 1569 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3990 4873 6023 6040 6193 6378 6823
Size: 1255 2410 883 1150 17 153 185 445
Cuts at: 6823 325
Size: 1498
Fragments arranged by size:
2410 1498 1255 1150 883 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3430 6242 7954
Size: 274 80 33 51 1443 1453 2812 1712
Cuts at: 7954 96
Size: 138
Fragments arranged by size:
2812 1712 1453 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7956 7988 7956
Size: 32 7964
Tth111I GACn'n_nGTC
Cuts at: 1316 3974 1316
Size: 2658 5338
XbaI T'CTAG_A
Cuts at: 5332 5332
Size: 7996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2653 2653
Size: 7996
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7996
XmaI C'CCGG_G
Cuts at: 1870 5321 1870
Size: 3451 4545
XmnI GAAnn'nnTTC
Cuts at: 4242 6125 4242
Size: 1883 6113
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5804 276
Size: 53 83 186 936 4270 2468
Fragments arranged by size:
4270 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI