Restriction map for
pFN21AB7310
(Circular) (Six-base) MAPSORT of: pFN21AB7310.seq Check: 3463 from: 1 to: 9529
pFN21AB7310 9529 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4109 4382 4531 5398 5576 5767 4109
Size: 273 149 867 178 191 7871
Fragments arranged by size:
7871 867 273 191 178 149
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7339 278
Size: 53 83 186 936 5803 2468
Fragments arranged by size:
5803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3108 5361 6872 1937
Size: 1171 2253 1511 4594
Fragments arranged by size:
4594 2253 1511 1171
Acc65I G'GTAC_C
Cuts at: 5057 6850 5057
Size: 1793 7736
AclI AA'CG_TT
Cuts at: 1563 7656 8029 1563
Size: 6093 373 3063
Fragments arranged by size:
6093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2617 4496 5808 6038 7592 8976 9244 1632
Size: 985 1879 1312 230 1554 1384 268 1917
Fragments arranged by size:
1917 1879 1554 1384 1312 985 268 230
AfeI AGC'GCT
Cuts at: 6077 6077
Size: 9529
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9332
AflIII A'CryG_T
Cuts at: 1203 8428 9405 1203
Size: 7225 977 1327
Fragments arranged by size:
7225 1327 977
AgeI A'CCGG_T
Cuts at: 8360 8360
Size: 9529
AhdI GACnn_n'nnGTC
Cuts at: 4201 8258 4201
Size: 4057 5472
AleI CACnn'nnGTG
Cuts at: 1166 4025 4052 5021 1166
Size: 2859 27 969 5674
Fragments arranged by size:
5674 2859 969 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4070 4461 4817 4887 1184
Size: 2886 391 356 70 5826
Fragments arranged by size:
5826 2886 391 356 70
AlwNI CAG_nnn'CTG
Cuts at: 3009 3130 3215 3895 4397 4541 4756 4828 4859
Size: 121 85 680 502 144 215 72 31
Cuts at: 4859 5170 5426 5626 5666 5851 6139 7272 8844
Size: 311 256 200 40 185 288 1133 1572
Cuts at: 8844 3009
Size: 3694
Fragments arranged by size:
3694 1572 1133 680 502 311 288 256
215 200 185 144 121 85 72 40
31
ApaI G_GGCC'C
Cuts at: 2653 2733 3555 3713 3982 4321 4360 5106 5145
Size: 80 822 158 269 339 39 746 39
Cuts at: 5145 5417 5789 6421 6674 2653
Size: 272 372 632 253 5508
Fragments arranged by size:
5508 822 746 632 372 339 272 269
253 158 80 39 39
ApaLI G'TGCA_C
Cuts at: 7586 8742 7586
Size: 1156 8373
ApoI r'AATT_y
Cuts at: 1493 5807 6838 7064 7216 9325 1493
Size: 4314 1031 226 152 2109 1697
Fragments arranged by size:
4314 2109 1697 1031 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8083 160
Size: 872 7051 1606
Fragments arranged by size:
7051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9529
AvaI C'yCGr_G
Cuts at: 1870 1943 3060 3267 4149 4306 4735 6153 6215
Size: 73 1117 207 882 157 429 1418 62
Cuts at: 6215 6854 1870
Size: 639 4545
Fragments arranged by size:
4545 1418 1117 882 639 429 207 157
73 62
BamHI G'GATC_C
Cuts at: 3559 3784 6859 7226 3559
Size: 225 3075 367 5862
Fragments arranged by size:
5862 3075 367 225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2643 3227 3380 4121
Size: 325 217 627 350 506 584 153 741
Cuts at: 4121 4153 4270 4301 4445 4519 4871 4909 4936
Size: 32 117 31 144 74 352 38 27
Cuts at: 4936 5057 5588 6376 6629 6850 8305 618
Size: 121 531 788 253 221 1455 1842
Fragments arranged by size:
1842 1455 788 741 627 584 531 506
352 350 325 253 221 217 153 144
121 117 74 38 32 31 27
BanII G_rGCy'C
Cuts at: 1391 2321 2418 2653 2733 2949 2981 3196 3546
Size: 930 97 235 80 216 32 215 350
Cuts at: 3546 3555 3665 3713 3982 4166 4321 4360 4385
Size: 9 110 48 269 184 155 39 25
Cuts at: 4385 4844 4952 5106 5114 5145 5417 5789 6170
Size: 459 108 154 8 31 272 372 381
Cuts at: 6170 6276 6421 6674 6778 6848 1391
Size: 106 145 253 104 70 4072
Fragments arranged by size:
4072 930 459 381 372 350 272 269
253 235 216 215 184 155 154 145
110 108 106 104 97 80 70 48
39 32 31 25 9 8
BbeI G_GCGC'C
Cuts at: 4274 4274
Size: 9529
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6029 6256 928
Size: 971 4130 227 4201
Fragments arranged by size:
4201 4130 971 227
BbvCI CC'TCA_GC
Cuts at: 3631 4095 5317 5763 6597 3631
Size: 464 1222 446 834 6563
Fragments arranged by size:
6563 1222 834 464 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7720 7754 1903
Size: 34 5783 34 3678
Fragments arranged by size:
5783 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7421 8631 7421
Size: 1210 8319
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3096 3236 3550
Size: 107 122 71 631 605 1424 140 314
Cuts at: 3550 3977 4355 5507 6485 8140 136
Size: 427 378 1152 978 1655 1525
Fragments arranged by size:
1655 1525 1424 1152 978 631 605 427
378 314 140 122 107 71
BglII A'GATC_T
Cuts at: 9524 9524
Size: 9529
BlpI GC'TnA_GC
Cuts at: 3049 4744 6946 3049
Size: 1695 2202 5632
Fragments arranged by size:
5632 2202 1695
Bme1580I G_kGCm'C
Cuts at: 1790 2533 2653 2733 2880 2918 3555 3713 3948
Size: 743 120 80 147 38 637 158 235
Cuts at: 3948 3982 4059 4142 4259 4304 4321 4360 4450
Size: 34 77 83 117 45 17 39 90
Cuts at: 4450 4613 4876 5043 5106 5145 5232 5417 5789
Size: 163 263 167 63 39 87 185 372
Cuts at: 5789 6421 6674 7590 8746 1790
Size: 632 253 916 1156 2573
Fragments arranged by size:
2573 1156 916 743 637 632 372 263
253 235 185 167 163 158 147 120
117 90 87 83 80 77 63 45
39 39 38 34 17
BmgBI CAC'GTC
Cuts at: 1318 6349 1318
Size: 5031 4498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2192 2512 3178
Size: 416 53 83 399 24 775 320 666
Cuts at: 3178 3252 3668 4152 4699 5226 5633 7260 8218
Size: 74 416 484 547 527 407 1627 958
Cuts at: 8218 442
Size: 1753
Fragments arranged by size:
1753 1627 958 775 666 547 527 484
416 416 407 399 320 83 74 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9529
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 9529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 3180 3851 5648 5873 5883 6241 6612
Size: 1080 861 671 1797 225 10 358 371
Cuts at: 6612 8189 1239
Size: 1577 2579
Fragments arranged by size:
2579 1797 1577 1080 861 671 371 358
225 10
Bpu10I CC'TnA_GC
Cuts at: 2286 2975 3540 3631 3664 4095 4384 4946 5317
Size: 689 565 91 33 431 289 562 371
Cuts at: 5317 5763 6597 2286
Size: 446 834 5218
Fragments arranged by size:
5218 834 689 565 562 446 431 371
289 91 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3383 5693 7654 8519 8817 3383
Size: 2310 1961 865 298 4095
Fragments arranged by size:
4095 2310 1961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3802 4152 5708 5786 8192 882
Size: 617 2303 350 1556 78 2406 2219
Fragments arranged by size:
2406 2303 2219 1556 617 350 78
BsaAI yAC'GTr
Cuts at: 493 1204 7320 493
Size: 711 6116 2702
Fragments arranged by size:
6116 2702 711
BsaBI GATnn'nnATC
Cuts at: 9523 9523
Size: 9529
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4100 4271 7336 7718
Size: 53 83 186 936 2567 171 3065 382
Cuts at: 7718 8353 275
Size: 635 1451
Fragments arranged by size:
3065 2567 1451 936 635 382 186 171
83 53
BsaWI w'CCGG_w
Cuts at: 2685 7962 8360 8634 8781 9192 2685
Size: 5277 398 274 147 411 3022
Fragments arranged by size:
5277 3022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2977 3007 3927 3957 5259
Size: 30 165 30 1574 30 920 30 1302
Cuts at: 5259 5289 5707 5737 6350 6380 1178
Size: 30 418 30 613 30 4327
Fragments arranged by size:
4327 1574 1302 920 613 418 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2405 3332 3353 3595 3619 3888 3932
Size: 1216 3 927 21 242 24 269 44
Cuts at: 3932 4993 5305 5780 5868 6122 6376 6594 1186
Size: 1061 312 475 88 254 254 218 4121
Fragments arranged by size:
4121 1216 1061 927 475 312 269 254
254 242 218 88 44 24 21 3
BseYI C'CCAG_C
Cuts at: 2141 2178 3737 3866 3982 4171 4252 4397 4460
Size: 37 1559 129 116 189 81 145 63
Cuts at: 4460 4503 4528 4562 4749 4875 4883 4925 4951
Size: 43 25 34 187 126 8 42 26
Cuts at: 4951 5137 5144 5238 5331 5440 5462 5511 5519
Size: 186 7 94 93 109 22 49 8
Cuts at: 5519 5813 5882 5893 6656 6689 6765 6780 8732
Size: 294 69 11 763 33 76 15 1952
Cuts at: 8732 9228 2141
Size: 496 2442
Fragments arranged by size:
2442 1952 1559 763 496 294 189 187
186 145 129 126 116 109 94 93
81 76 69 63 49 43 42 37
34 33 26 25 22 15 11 8
8 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 4557 4629 4742 4883 5045 5183
Size: 573 447 2374 72 113 141 162 138
Cuts at: 5183 5277 5289 5595 1163
Size: 94 12 306 5097
Fragments arranged by size:
5097 2374 573 447 306 162 141 138
113 94 72 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6979 7740 8768 1540
Size: 275 186 4978 761 1028 2301
Fragments arranged by size:
4978 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2949 2981 3196 4922 5114 7590 7675 8746 2949
Size: 32 215 1726 192 2476 85 1071 3732
Fragments arranged by size:
3732 2476 1726 1071 215 192 85 32
BsiWI C'GTAC_G
Cuts at: 5097 5097
Size: 9529
BsmI GAATG_Cn'
Cuts at: 2623 5562 7040 7133 2623
Size: 2939 1478 93 5019
Fragments arranged by size:
5019 2939 1478 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2879 2903 4107 2879
Size: 24 1204 8301
Fragments arranged by size:
8301 1204 24
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2418 2533 2653 2733 2880 2918
Size: 399 531 97 115 120 80 147 38
Cuts at: 2918 2949 2981 3196 3546 3555 3665 3713 3948
Size: 31 32 215 350 9 110 48 235
Cuts at: 3948 3982 4059 4142 4166 4259 4304 4321 4360
Size: 34 77 83 24 93 45 17 39
Cuts at: 4360 4385 4450 4613 4844 4876 4922 4952 5043
Size: 25 65 163 231 32 46 30 91
Cuts at: 5043 5106 5114 5145 5232 5417 5789 6170 6276
Size: 63 8 31 87 185 372 381 106
Cuts at: 6276 6421 6674 6778 6848 7590 7675 8746 1391
Size: 145 253 104 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 531 399 381 372 350
253 235 231 215 185 163 147 145
120 115 110 106 104 97 93 91
87 85 83 80 77 70 65 63
48 46 45 39 38 34 32 32
31 31 30 25 24 17 9 8
BspEI T'CCGG_A
Cuts at: 9192 9192
Size: 9529
BspHI T'CATG_A
Cuts at: 2248 2625 7418 9148 2248
Size: 377 4793 1730 2629
Fragments arranged by size:
4793 2629 1730 377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 2233 4109 4382 4531 5398 5465 5576
Size: 1350 39 1876 273 149 867 67 111
Cuts at: 5576 5767 6884 844
Size: 191 1117 3489
Fragments arranged by size:
3489 1876 1350 1117 867 273 191 149
111 67 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 2896 3412 5770 7416 1394
Size: 429 467 606 516 2358 1646 3507
Fragments arranged by size:
3507 2358 1646 606 516 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3360 4344 8024 8198 65
Size: 1183 2112 984 3680 174 1396
Fragments arranged by size:
3680 2112 1396 1183 984 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4817 8173 8360 1700
Size: 110 3007 3356 187 2869
Fragments arranged by size:
3356 3007 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9529
BssSI C'ACGA_G
Cuts at: 2854 5107 7589 8601 2854
Size: 2253 2482 1012 3782
Fragments arranged by size:
3782 2482 2253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 5047 5170 794
Size: 1449 2804 123 5153
Fragments arranged by size:
5153 2804 1449 123
BstBI TT'CG_AA
Cuts at: 6069 8333 6069
Size: 2264 7265
BstEII G'GTnAC_C
Cuts at: 1184 3415 4018 1184
Size: 2231 603 6695
Fragments arranged by size:
6695 2231 603
BstXI CCAn_nnnn'nTGG
Cuts at: 2860 3689 4933 5718 6202 2860
Size: 829 1244 785 484 6187
Fragments arranged by size:
6187 1244 829 785 484
BstYI r'GATC_y
Cuts at: 1972 2481 2700 3559 3784 6534 6859 7226 7611
Size: 509 219 859 225 2750 325 367 385
Cuts at: 7611 7628 9080 9166 9178 9524 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2750 1977 1452 859 509 385 367 346
325 225 219 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6105 6105
Size: 9529
BtgI C'CryG_G
Cuts at: 513 1065 2053 2382 4014 5568 5727 5796 6195
Size: 552 988 329 1632 1554 159 69 399
Cuts at: 6195 6445 6576 513
Size: 250 131 3466
Fragments arranged by size:
3466 1632 1554 988 552 399 329 250
159 131 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3838 4045 5539 487
Size: 683 56 2612 207 1494 4477
Fragments arranged by size:
4477 2612 1494 683 207 56
BtsI GCAGTG_nn'
Cuts at: 2916 3517 4374 4641 4826 5192 5430 5747 5941
Size: 601 857 267 185 366 238 317 194
Cuts at: 5941 6725 7045 7839 7859 2916
Size: 784 320 794 20 4586
Fragments arranged by size:
4586 857 794 784 601 366 320 317
267 238 194 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2325 2360 2783 2818 3373 3408 529
Size: 35 1761 35 423 35 555 35 6650
Fragments arranged by size:
6650 1761 555 423 35 35 35 35
DraI TTT'AAA
Cuts at: 6833 6833
Size: 9529
DraIII CAC_nnn'GTG
Cuts at: 2200 2264 4237 2200
Size: 64 1973 7492
Fragments arranged by size:
7492 1973 64
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5834 6047 8536 809
Size: 507 4518 213 2489 1802
Fragments arranged by size:
4518 2489 1802 507 213
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2255 3237 3291
Size: 54 1425 50 275 18 425 982 54
Cuts at: 3291 4087 4782 4819 5370 5799 6141 6976 7865
Size: 796 695 37 551 429 342 835 889
Cuts at: 7865 9494 8
Size: 1629 43
Fragments arranged by size:
1629 1425 982 889 835 796 695 551
429 425 342 275 54 54 50 43
37 18
EagI C'GGCC_G
Cuts at: 1537 1812 6976 1537
Size: 275 5164 4090
Fragments arranged by size:
5164 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6066 6176 7459 1353
Size: 4713 110 1283 3423
Fragments arranged by size:
4713 3423 1283 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2361 3114 3583 3940 3948 4675
Size: 1064 138 772 753 469 357 8 727
Cuts at: 4675 8116 8490 8636 387
Size: 3441 374 146 1280
Fragments arranged by size:
3441 1280 1064 772 753 727 469 374
357 146 138 8
EcoICRI GAG'CTC
Cuts at: 2947 2979 3194 5112 2947
Size: 32 215 1918 7364
Fragments arranged by size:
7364 1918 215 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 2617 3180 3851 4496 5648 5808
Size: 393 687 298 563 671 645 1152 160
Cuts at: 5808 5873 5883 6038 6241 6612 7592 8189 8976
Size: 65 10 155 203 371 980 597 787
Cuts at: 8976 9244 1239
Size: 268 1524
Fragments arranged by size:
1524 1152 980 787 687 671 645 597
563 393 371 298 268 203 160 155
65 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 4454 2537
Size: 1917 7612
EcoO109I rG'GnC_Cy
Cuts at: 2022 2324 2650 2729 3041 3200 3451 3551 3552
Size: 302 326 79 312 159 251 100 1
Cuts at: 3552 3710 3842 3978 3979 4317 4318 4356 4357
Size: 158 132 136 1 338 1 38 1
Cuts at: 4357 4726 4768 4990 5141 5270 5344 5413 5530
Size: 369 42 222 151 129 74 69 117
Cuts at: 5530 5574 5785 5786 5848 6049 6220 6418 6670
Size: 44 211 1 62 201 171 198 252
Cuts at: 6670 6671 6749 6803 2022
Size: 1 78 54 4748
Fragments arranged by size:
4748 369 338 326 312 302 252 251
222 211 201 198 171 159 158 151
136 132 129 117 100 79 78 74
69 62 54 44 42 38 1 1
1 1 1 1
EcoRI G'AATT_C
Cuts at: 6838 7216 6838
Size: 378 9151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6043 6043
Size: 9529
FseI GG_CCGG'CC
Cuts at: 4821 4821
Size: 9529
FspI TGC'GCA
Cuts at: 8035 8035
Size: 9529
HaeII r_GCGC'y
Cuts at: 1678 3122 4274 5469 5781 6079 6415 8676 1678
Size: 1444 1152 1195 312 298 336 2261 2531
Fragments arranged by size:
2531 2261 1444 1195 1152 336 312 298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2936 2968 3867 3899 4187
Size: 32 135 32 1364 32 899 32 288
Cuts at: 4187 4219 5259 5291 5707 5739 6212 6244 6668
Size: 32 1040 32 416 32 473 32 424
Cuts at: 6668 6700 8170 8202 8244 8276 1373
Size: 32 1470 32 42 32 2626
Fragments arranged by size:
2626 1470 1364 1040 899 473 424 416
288 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6873 7119 669
Size: 988 281 4935 246 3079
Fragments arranged by size:
4935 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9529
HpaI GTT'AAC
Cuts at: 7119 7119
Size: 9529
KasI G'GCGC_C
Cuts at: 4270 4270
Size: 9529
KpnI G_GTAC'C
Cuts at: 5061 6854 5061
Size: 1793 7736
MfeI C'AATT_G
Cuts at: 7128 7128
Size: 9529
MluI A'CGCG_T
Cuts at: 9405 9405
Size: 9529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 2492 2886 3458 8643 8827 1305
Size: 927 260 394 572 5185 184 2007
Fragments arranged by size:
5185 2007 927 572 394 260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5372 5801 6143 10
Size: 54 1425 343 3540 429 342 3396
Fragments arranged by size:
3540 3396 1425 429 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2451 4025 4052 4799 5021 5567
Size: 648 903 382 1574 27 747 222 546
Cuts at: 5567 7487 7846 8005 518
Size: 1920 359 159 2042
Fragments arranged by size:
2042 1920 1574 903 747 648 546 382
359 222 159 27
MspA1I CmG'CkG
Cuts at: 1606 2240 3078 3256 3707 3870 4171 4556 4566
Size: 634 838 178 451 163 301 385 10
Cuts at: 4566 4753 4900 4929 5033 5148 5242 5515 5903
Size: 187 147 29 104 115 94 273 388
Cuts at: 5903 6261 6635 6815 6945 7622 8770 9015 1606
Size: 358 374 180 130 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 677 634 451 388 385
374 358 301 273 245 187 180 178
163 147 130 115 104 94 29 10
NaeI GCC'GGC
Cuts at: 4819 4819
Size: 9529
NarI GG'CG_CC
Cuts at: 4271 4271
Size: 9529
NcoI C'CATG_G
Cuts at: 513 1065 4014 5796 6445 513
Size: 552 2949 1782 649 3597
Fragments arranged by size:
3597 2949 1782 649 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9529
NgoMIV G'CCGG_C
Cuts at: 4817 4817
Size: 9529
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9529
NotI GC'GGCC_GC
Cuts at: 6976 6976
Size: 9529
NspI r_CATG'y
Cuts at: 1132 1229 2068 2310 2905 4798 6887 8432 1132
Size: 97 839 242 595 1893 2089 1545 2229
Fragments arranged by size:
2229 2089 1893 1545 839 595 242 97
PasI CC'CwG_GG
Cuts at: 1348 2725 2930 3548 3731 4351 4426 4450 4541
Size: 1377 205 618 183 620 75 24 91
Cuts at: 4541 5053 5840 1348
Size: 512 787 5037
Fragments arranged by size:
5037 1377 787 620 618 512 205 183
91 75 24
PciI A'CATG_T
Cuts at: 8428 8428
Size: 9529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2200 2264 2758 3731 4541 1108
Size: 1092 64 494 973 810 6096
Fragments arranged by size:
6096 1092 973 810 494 64
PfoI T'CCnGG_A
Cuts at: 3196 3196
Size: 9529
PmeI GTTT'AAAC
Cuts at: 6833 6833
Size: 9529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7598 7630 9111 9143 7598
Size: 32 1481 32 7984
Fragments arranged by size:
7984 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2324 3041 3200 3451 4768 4990 5270 5574 6049
Size: 717 159 251 1317 222 280 304 475
Cuts at: 6049 6220 6803 2324
Size: 171 583 5050
Fragments arranged by size:
5050 1317 717 583 475 304 280 251
222 171 159
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9529
PsiI TTA'TAA
Cuts at: 7099 7099
Size: 9529
PspOMI G'GGCC_C
Cuts at: 2649 2729 3551 3709 3978 4317 4356 5102 5141
Size: 80 822 158 269 339 39 746 39
Cuts at: 5141 5413 5785 6417 6670 2649
Size: 272 372 632 253 5508
Fragments arranged by size:
5508 822 746 632 372 339 272 269
253 158 80 39 39
PstI C_TGCA'G
Cuts at: 830 3891 4674 5395 6135 6266 6528 6881 830
Size: 3061 783 721 740 131 262 353 3478
Fragments arranged by size:
3478 3061 783 740 721 353 262 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9529
PvuII CAG'CTG
Cuts at: 2240 4753 4900 5242 5903 6261 6815 2240
Size: 2513 147 342 661 358 554 4954
Fragments arranged by size:
4954 2513 661 554 358 342 147
RsrII CG'GwC_CG
Cuts at: 2688 2688
Size: 9529
SacI G_AGCT'C
Cuts at: 2949 2981 3196 5114 2949
Size: 32 215 1918 7364
Fragments arranged by size:
7364 1918 215 32
SalI G'TCGA_C
Cuts at: 1936 6871 1936
Size: 4935 4594
SapI GCTCTTCn'nnn_
Cuts at: 6066 6066
Size: 9529
SbfI CC_TGCA'GG
Cuts at: 6881 6881
Size: 9529
ScaI AGT'ACT
Cuts at: 7777 9489 7777
Size: 1712 7817
SexAI A'CCwGG_T
Cuts at: 2260 4441 2260
Size: 2181 7348
SfcI C'TryA_G
Cuts at: 826 1045 3122 3567 3887 4670 5115 5391 5488
Size: 219 2077 445 320 783 445 276 97
Cuts at: 5488 5904 6131 6262 6524 6877 8012 8693 8884
Size: 416 227 131 262 353 1135 681 191
Cuts at: 8884 826
Size: 1471
Fragments arranged by size:
2077 1471 1135 783 681 445 445 416
353 320 276 262 227 219 191 131
97
SfiI GGCCn_nnn'nGGCC
Cuts at: 3977 5507 3977
Size: 1530 7999
SfoI GGC'GCC
Cuts at: 4272 4272
Size: 9529
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9529
SmaI CCC'GGG
Cuts at: 1872 4308 4737 6155 6856 1872
Size: 2436 429 1418 701 4545
Fragments arranged by size:
4545 2436 1418 701 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3398 5672 7633 8534 8796 820
Size: 197 926 1455 2274 1961 901 262 1553
Fragments arranged by size:
2274 1961 1553 1455 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7320 493
Size: 6827 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9529
SphI G_CATG'C
Cuts at: 1132 6887 1132
Size: 5755 3774
SspI AAT'ATT
Cuts at: 5 52 7453 5
Size: 47 7401 2081
Fragments arranged by size:
7401 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 1517
Size: 879 8650
StyI C'CwwG_G
Cuts at: 513 1065 2111 2538 2733 2988 3231 3759 4014
Size: 552 1046 427 195 255 243 528 255
Cuts at: 4014 4548 5409 5505 5781 5796 6445 6513 6968
Size: 534 861 96 276 15 649 68 455
Cuts at: 6968 513
Size: 3074
Fragments arranged by size:
3074 1046 861 649 552 534 528 455
427 276 255 255 243 195 96 68
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2155 2211 2811 2838 3434 3812 4163
Size: 1255 575 56 600 27 596 378 351
Cuts at: 4163 4487 4591 4663 4939 5323 6703 7556 7573
Size: 324 104 72 276 384 1380 853 17
Cuts at: 7573 7726 7911 8356 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1380 1255 853 600 596 575 445
384 378 351 324 276 185 153 104
72 56 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4482 7775 9487
Size: 274 80 33 51 1443 2505 3293 1712
Cuts at: 9487 96
Size: 138
Fragments arranged by size:
3293 2505 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 2977 3009 3483 3515 3927 3959 9489
Size: 32 685 32 474 32 412 32 5530
Cuts at: 9489 9521 2260
Size: 32 2268
Fragments arranged by size:
5530 2268 685 474 412 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2518 1316
Size: 1202 8327
XbaI T'CTAG_A
Cuts at: 6865 6865
Size: 9529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2805 4132 4358 4757 5566 2197
Size: 608 1327 226 399 809 6160
Fragments arranged by size:
6160 1327 809 608 399 226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9529
XmaI C'CCGG_G
Cuts at: 1870 4306 4735 6153 6854 1870
Size: 2436 429 1418 701 4545
Fragments arranged by size:
4545 2436 1418 701 429
XmnI GAAnn'nnTTC
Cuts at: 2621 7658 2621
Size: 5037 4492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7337 276
Size: 53 83 186 936 5803 2468
Fragments arranged by size:
5803 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI
NruI NsiI PacI PmlI PspXI PsrI SacII SanDI
SrfI SwaI