Restriction map for
pFN21AB7308
(Circular) (Six-base) MAPSORT of: pFN21AB7308.seq Check: 441 from: 1 to: 7171
pFN21AB7308 7171 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2320 2320
Size: 7171
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3903 4045 4514 1937
Size: 1966 142 469 4594
Fragments arranged by size:
4594 1966 469 142
Acc65I G'GTAC_C
Cuts at: 3384 4492 3384
Size: 1108 6063
AclI AA'CG_TT
Cuts at: 1563 2936 5298 5671 1563
Size: 1373 2362 373 3063
Fragments arranged by size:
3063 2362 1373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2858 3144 3312 4249 4418 5234 6618
Size: 413 813 286 168 937 169 816 1384
Cuts at: 6618 6886 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 937 816 813 413 286 268
169 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6974
AflIII A'CryG_T
Cuts at: 1203 3540 3779 4230 6070 7047 1203
Size: 2337 239 451 1840 977 1327
Fragments arranged by size:
2337 1840 1327 977 451 239
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 3899 5900 3899
Size: 2001 5170
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4087 1184
Size: 2903 4268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4201 4233 4201
Size: 32 7139
AlwNI CAG_nnn'CTG
Cuts at: 3112 4914 6486 3112
Size: 1802 1572 3797
Fragments arranged by size:
3797 1802 1572
ApaLI G'TGCA_C
Cuts at: 5228 6384 5228
Size: 1156 6015
ApoI r'AATT_y
Cuts at: 1493 2029 2093 2446 3263 3721 3815 3834 3992
Size: 536 64 353 817 458 94 19 158
Cuts at: 3992 4480 4706 4858 6967 1493
Size: 488 226 152 2109 1697
Fragments arranged by size:
2109 1697 817 536 488 458 353 226
158 152 94 64 19
AseI AT'TA_AT
Cuts at: 160 1032 2432 5725 160
Size: 872 1400 3293 1606
Fragments arranged by size:
3293 1606 1400 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 4028 4496 1870
Size: 73 2085 468 4545
Fragments arranged by size:
4545 2085 468 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3376 3409 3376
Size: 33 7138
BamHI G'GATC_C
Cuts at: 4501 4868 4501
Size: 367 6804
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3384 4492 5947 618
Size: 325 217 627 1597 1108 1455 1842
Fragments arranged by size:
1842 1597 1455 1108 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3326 4446 4490 1391
Size: 1935 1120 44 4072
Fragments arranged by size:
4072 1935 1120 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2267 928
Size: 971 368 5832
Fragments arranged by size:
5832 971 368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5362 5396 1903
Size: 34 3425 34 3678
Fragments arranged by size:
3678 3425 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2215 4325 5063 6273 2215
Size: 2110 738 1210 3113
Fragments arranged by size:
3113 2110 1210 738
BclI T'GATC_A
Cuts at: 1548 2379 3850 1548
Size: 831 1471 4869
Fragments arranged by size:
4869 1471 831
BfrBI ATG'CAT
Cuts at: 2132 2132
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5782 136
Size: 107 122 71 631 605 4110 1525
Fragments arranged by size:
4110 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3790 7166 3790
Size: 3376 3795
BlpI GC'TnA_GC
Cuts at: 3065 4588 3065
Size: 1523 5648
Bme1580I G_kGCm'C
Cuts at: 1790 2581 3560 3746 5232 6388 1790
Size: 791 979 186 1486 1156 2573
Fragments arranged by size:
2573 1486 1156 979 791 186
BmgBI CAC'GTC
Cuts at: 1318 4231 1318
Size: 2913 4258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 3329 3570
Size: 416 53 83 399 24 654 1258 241
Cuts at: 3570 3719 4902 5860 442
Size: 149 1183 958 1753
Fragments arranged by size:
1753 1258 1183 958 654 416 399 241
149 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3180 5831 1239
Size: 1941 2651 2579
Fragments arranged by size:
2651 2579 1941
Bpu10I CC'TnA_GC
Cuts at: 4270 4270
Size: 7171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3399 3824 5296 6161 6459 3399
Size: 425 1472 865 298 4111
Fragments arranged by size:
4111 1472 865 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3309 4253 5834 882
Size: 617 1810 944 1581 2219
Fragments arranged by size:
2219 1810 1581 944 617
BsaAI yAC'GTr
Cuts at: 493 1204 4962 493
Size: 711 3758 2702
Fragments arranged by size:
3758 2702 711
BsaBI GATnn'nnATC
Cuts at: 3350 7165 3350
Size: 3815 3356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4978 5360 5995 275
Size: 53 83 186 936 3445 382 635 1451
Fragments arranged by size:
3445 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4189 4335 5604 6002 6276 6423 6834 4189
Size: 146 1269 398 274 147 411 4526
Fragments arranged by size:
4526 1269 411 398 274 147 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4201 4231 1178
Size: 30 165 30 2798 30 4118
Fragments arranged by size:
4118 2798 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2999 3523 1186
Size: 1813 524 4834
Fragments arranged by size:
4834 1813 524
BseYI C'CCAG_C
Cuts at: 4350 6374 6870 4350
Size: 2024 496 4651
Fragments arranged by size:
4651 2024 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3300 1163
Size: 573 1564 5034
Fragments arranged by size:
5034 1564 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4621 5382 6410 1540
Size: 275 186 2620 761 1028 2301
Fragments arranged by size:
2620 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4446 5232 5317 6388 4446
Size: 786 85 1071 5229
Fragments arranged by size:
5229 1071 786 85
BsmI GAATG_Cn'
Cuts at: 4682 4775 4682
Size: 93 7078
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2581 3326 3560 3746 4446 4490 5232
Size: 399 791 745 234 186 700 44 742
Cuts at: 5232 5317 6388 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 791 745 742 700 399 234
186 85 44
BspEI T'CCGG_A
Cuts at: 6834 6834
Size: 7171
BspHI T'CATG_A
Cuts at: 3682 4380 5060 6790 3682
Size: 698 680 1730 4063
Fragments arranged by size:
4063 1730 698 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 4526 844
Size: 1476 2206 3489
Fragments arranged by size:
3489 2206 1476
BsrBI CCG'CTC
Cuts at: 1394 1823 5058 1394
Size: 429 3235 3507
Fragments arranged by size:
3507 3235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5666 5840 65
Size: 1183 4418 174 1396
Fragments arranged by size:
4418 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5815 6002 1700
Size: 110 4005 187 2869
Fragments arranged by size:
4005 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 5231 6243 5231
Size: 1012 6159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3166 794
Size: 2372 4799
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 2386 1184
Size: 1202 5969
BstYI r'GATC_y
Cuts at: 1972 2767 3790 4501 4868 5253 5270 6722 6808
Size: 795 1023 711 367 385 17 1452 86
Cuts at: 6808 6820 7166 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1023 795 711 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 4046 4046
Size: 7171
Bsu36I CC'TnA_GG
Cuts at: 3657 3798 3657
Size: 141 7030
BtgI C'CryG_G
Cuts at: 513 1065 2004 3388 513
Size: 552 939 1384 4296
Fragments arranged by size:
4296 1384 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 4687 5481 5501 2049
Size: 2638 794 20 3719
Fragments arranged by size:
3719 2638 794 20
ClaI AT'CG_AT
Cuts at: 1554 3547 1554
Size: 1993 5178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 2976 4475 2976
Size: 1499 5672
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6178 809
Size: 507 4862 1802
Fragments arranged by size:
4862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4618 5507 7136
Size: 54 1425 50 275 18 2788 889 1629
Cuts at: 7136 8
Size: 43
Fragments arranged by size:
2788 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4618 1537
Size: 275 2806 4090
Fragments arranged by size:
4090 2806 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3002 3331 5101 1353
Size: 1649 329 1770 3423
Fragments arranged by size:
3423 1770 1649 329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5758 6132 6278 387
Size: 1064 138 4169 374 146 1280
Fragments arranged by size:
4169 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4444 4444
Size: 7171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2858 3144 3180 3312 4249 4418
Size: 393 413 813 286 36 132 937 169
Cuts at: 4418 5234 5831 6618 6886 1239
Size: 816 597 787 268 1524
Fragments arranged by size:
1524 937 816 813 787 597 413 393
286 268 169 132 36
EcoO109I rG'GnC_Cy
Cuts at: 4054 4054
Size: 7171
EcoRI G'AATT_C
Cuts at: 2029 2446 4480 4858 2029
Size: 417 2034 378 4342
Fragments arranged by size:
4342 2034 417 378
EcoRV GAT'ATC
Cuts at: 2608 2608
Size: 7171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3809 3809
Size: 7171
FspI TGC'GCA
Cuts at: 5677 5677
Size: 7171
HaeII r_GCGC'y
Cuts at: 1678 6318 1678
Size: 4640 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 2689 2721 3029
Size: 32 135 32 695 32 390 32 308
Cuts at: 3029 3061 3209 3241 3242 3274 3332 3364 3504
Size: 32 148 32 1 32 58 32 140
Cuts at: 3504 3536 3664 3696 3885 3917 4330 4362 5812
Size: 32 128 32 189 32 413 32 1450
Cuts at: 5812 5844 5886 5918 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1450 695 413 390 308 189 148
140 135 128 58 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4515 4761 669
Size: 988 281 2577 246 3079
Fragments arranged by size:
3079 2577 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2472 748
Size: 1724 5447
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 3388 4496 3388
Size: 1108 6063
MfeI C'AATT_G
Cuts at: 3285 4770 3285
Size: 1485 5686
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6285 6469 1305
Size: 4980 184 2007
Fragments arranged by size:
4980 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5349
Fragments arranged by size:
5349 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3687 3911 5129 5488 5647 518
Size: 648 1143 1378 224 1218 359 159 2042
Fragments arranged by size:
2042 1378 1218 1143 648 359 224 159
MspA1I CmG'CkG
Cuts at: 1606 3112 4587 5264 6412 6657 1606
Size: 1506 1475 677 1148 245 2120
Fragments arranged by size:
2120 1506 1475 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3388 513
Size: 552 939 1384 4296
Fragments arranged by size:
4296 1384 939 552
NdeI CA'TA_TG
Cuts at: 387 2541 387
Size: 2154 5017
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NsiI A_TGCA'T
Cuts at: 2134 2134
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2270 3544 3783 4529 6074 1132
Size: 97 1041 1274 239 746 1545 2229
Fragments arranged by size:
2229 1545 1274 1041 746 239 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7171
PciI A'CATG_T
Cuts at: 3540 3779 6070 3540
Size: 239 2291 4641
Fragments arranged by size:
4641 2291 239
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2214 4179 1108
Size: 1106 1965 4100
Fragments arranged by size:
4100 1965 1106
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3960 3992 4201 4233 5240 5272 6753 6785 3960
Size: 32 209 32 1007 32 1481 32 4346
Fragments arranged by size:
4346 1481 1007 209 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4054 4054
Size: 7171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4401 4741 4401
Size: 340 6831
PstI C_TGCA'G
Cuts at: 830 4523 830
Size: 3693 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 3112 3112
Size: 7171
SacI G_AGCT'C
Cuts at: 4446 4446
Size: 7171
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SapI GCTCTTCn'nnn_
Cuts at: 3331 3331
Size: 7171
SbfI CC_TGCA'GG
Cuts at: 4523 4523
Size: 7171
ScaI AGT'ACT
Cuts at: 2176 5419 7131 2176
Size: 3243 1712 2216
Fragments arranged by size:
3243 2216 1712
SexAI A'CCwGG_T
Cuts at: 3469 3469
Size: 7171
SfcI C'TryA_G
Cuts at: 826 1045 2422 4519 5654 6335 6526 826
Size: 219 1377 2097 1135 681 191 1471
Fragments arranged by size:
2097 1471 1377 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 4498 1872
Size: 2626 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3414 3839 4028 5275 6176 6438
Size: 197 926 1471 425 189 1247 901 262
Cuts at: 6438 820
Size: 1553
Fragments arranged by size:
1553 1471 1247 926 901 425 262 197
189
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7171
SphI G_CATG'C
Cuts at: 1132 4529 1132
Size: 3397 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5095 5
Size: 47 3810 1233 2081
Fragments arranged by size:
3810 2081 1233 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7171
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 3388 4610 513
Size: 552 939 501 883 1222 3074
Fragments arranged by size:
3074 1222 939 883 552 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5198 5215 5368 5553 5998 325
Size: 1255 3618 17 153 185 445 1498
Fragments arranged by size:
3618 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2174 2774 5417
Size: 274 80 33 51 1443 197 600 2643
Cuts at: 5417 7129 96
Size: 1712 138
Fragments arranged by size:
2643 1712 1443 600 274 197 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3457 3489 7131 7163 3457
Size: 32 3642 32 3465
Fragments arranged by size:
3642 3465 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7171
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2398
Size: 7171
XhoI C'TCGA_G
Cuts at: 1943 4028 1943
Size: 2085 5086
XmaI C'CCGG_G
Cuts at: 1870 4496 1870
Size: 2626 4545
XmnI GAAnn'nnTTC
Cuts at: 2871 3273 3792 5300 2871
Size: 402 519 1508 4742
Fragments arranged by size:
4742 1508 519 402
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BplI BsiWI
BsmBI BstXI DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI