Restriction map for
pFN21AB7306
(Circular) (Six-base) MAPSORT of: pFN21AB7306.seq Check: 1925 from: 1 to: 7897
pFN21AB7306 7897 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3209 3419 3809 3209
Size: 210 390 7297
Fragments arranged by size:
7297 390 210
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5707 278
Size: 53 83 186 936 4171 2468
Fragments arranged by size:
4171 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5240 1937
Size: 3303 4594
Acc65I G'GTAC_C
Cuts at: 5218 5218
Size: 7897
AclI AA'CG_TT
Cuts at: 1563 6024 6397 1563
Size: 4461 373 3063
Fragments arranged by size:
4461 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2874 3066 5960 7344 7612 1632
Size: 1242 192 2894 1384 268 1917
Fragments arranged by size:
2894 1917 1384 1242 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7700
AflIII A'CryG_T
Cuts at: 1203 4322 4854 6796 7773 1203
Size: 3119 532 1942 977 1327
Fragments arranged by size:
3119 1942 1327 977 532
AgeI A'CCGG_T
Cuts at: 6728 6728
Size: 7897
AhdI GACnn_n'nnGTC
Cuts at: 6626 6626
Size: 7897
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7897
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3118 1184
Size: 1934 5963
AlwNI CAG_nnn'CTG
Cuts at: 3700 5640 7212 3700
Size: 1940 1572 4385
Fragments arranged by size:
4385 1940 1572
ApaI G_GGCC'C
Cuts at: 3176 3386 3176
Size: 210 7687
ApaLI G'TGCA_C
Cuts at: 2394 4814 5954 7110 2394
Size: 2420 1140 1156 3181
Fragments arranged by size:
3181 2420 1156 1140
ApoI r'AATT_y
Cuts at: 1493 2099 2150 2649 2793 2839 3497 3905 4556
Size: 606 51 499 144 46 658 408 651
Cuts at: 4556 4757 5206 5432 5584 7693 1493
Size: 201 449 226 152 2109 1697
Fragments arranged by size:
2109 1697 658 651 606 499 449 408
226 201 152 144 51 46
AseI AT'TA_AT
Cuts at: 160 1032 6451 160
Size: 872 5419 1606
Fragments arranged by size:
5419 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7897
AvaI C'yCGr_G
Cuts at: 1870 1943 4170 4371 5222 1870
Size: 73 2227 201 851 4545
Fragments arranged by size:
4545 2227 851 201 73
BamHI G'GATC_C
Cuts at: 5227 5594 5227
Size: 367 7530
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5218 6673 618
Size: 325 217 627 3431 1455 1842
Fragments arranged by size:
3431 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2263 3176 3386 5216 1391
Size: 872 913 210 1830 4072
Fragments arranged by size:
4072 1830 913 872 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2524 2573 3211 4009 4162 4491 4947
Size: 971 625 49 638 798 153 329 456
Cuts at: 4947 928
Size: 3878
Fragments arranged by size:
3878 971 798 638 625 456 329 153
49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6088 6122 1903
Size: 34 4151 34 3678
Fragments arranged by size:
4151 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4312 5789 6999 4312
Size: 1477 1210 5210
Fragments arranged by size:
5210 1477 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7897
BfrBI ATG'CAT
Cuts at: 2764 2980 3400 3577 2764
Size: 216 420 177 7084
Fragments arranged by size:
7084 420 216 177
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2754 3567 3975
Size: 107 122 71 631 605 1082 813 408
Cuts at: 3975 4626 6508 136
Size: 651 1882 1525
Fragments arranged by size:
1882 1525 1082 813 651 631 605 408
122 107 71
BglII A'GATC_T
Cuts at: 7892 7892
Size: 7897
BlpI GC'TnA_GC
Cuts at: 2598 5314 2598
Size: 2716 5181
Bme1580I G_kGCm'C
Cuts at: 1790 2398 2782 3176 3386 4818 5958 7114 1790
Size: 608 384 394 210 1432 1140 1156 2573
Fragments arranged by size:
2573 1432 1156 1140 608 394 384 210
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7897
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4805 5628 6586
Size: 416 53 83 399 24 3388 823 958
Cuts at: 6586 442
Size: 1753
Fragments arranged by size:
3388 1753 958 823 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4267 6557 1239
Size: 3028 2290 2579
Fragments arranged by size:
3028 2579 2290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4917 6022 6887 7185 4917
Size: 1105 865 298 5629
Fragments arranged by size:
5629 1105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6560 882
Size: 617 5061 2219
Fragments arranged by size:
5061 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2199 4190 5688 493
Size: 711 995 1991 1498 2702
Fragments arranged by size:
2702 1991 1498 995 711
BsaBI GATnn'nnATC
Cuts at: 4306 4637 7891 4306
Size: 331 3254 4312
Fragments arranged by size:
4312 3254 331
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5704 6086 6721 275
Size: 53 83 186 936 4171 382 635 1451
Fragments arranged by size:
4171 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6330 6728 7002 7149 7560 6330
Size: 398 274 147 411 6667
Fragments arranged by size:
6667 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3188 3218 3398 3428 3788
Size: 30 165 30 1785 30 180 30 360
Cuts at: 3788 3818 1178
Size: 30 5257
Fragments arranged by size:
5257 1785 360 180 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3320 4116 5159 1186
Size: 2134 796 1043 3924
Fragments arranged by size:
3924 2134 1043 796
BseYI C'CCAG_C
Cuts at: 7100 7596 7100
Size: 496 7401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3724 3925 4351 4576 4771 4879 1163
Size: 573 1988 201 426 225 195 108 4181
Fragments arranged by size:
4181 1988 573 426 225 201 195 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5347 6108 7136 1540
Size: 275 186 3346 761 1028 2301
Fragments arranged by size:
3346 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2263 2398 4818 5958 6043 7114 2263
Size: 135 2420 1140 85 1071 3046
Fragments arranged by size:
3046 2420 1140 1071 135 85
BsmI GAATG_Cn'
Cuts at: 2416 5408 5501 2416
Size: 2992 93 4812
Fragments arranged by size:
4812 2992 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2398 2782 3176 3386 4818 5216
Size: 399 473 135 384 394 210 1432 398
Cuts at: 5216 5958 6043 7114 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1432 1071 742 473 399 398 394
384 210 135 85
BspEI T'CCGG_A
Cuts at: 7560 7560
Size: 7897
BspHI T'CATG_A
Cuts at: 5089 5786 7516 5089
Size: 697 1730 5470
Fragments arranged by size:
5470 1730 697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3209 3419 3809 5252 844
Size: 2365 210 390 1443 3489
Fragments arranged by size:
3489 2365 1443 390 210
BsrBI CCG'CTC
Cuts at: 1394 1823 5784 1394
Size: 429 3961 3507
Fragments arranged by size:
3961 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2595 4537 6392 6566 65
Size: 1183 1347 1942 1855 174 1396
Fragments arranged by size:
1942 1855 1396 1347 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6541 6728 1700
Size: 110 4731 187 2869
Fragments arranged by size:
4731 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2720 96
Size: 2624 5273
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7897
BssSI C'ACGA_G
Cuts at: 3051 5957 6969 3051
Size: 2906 1012 3979
Fragments arranged by size:
3979 2906 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2402 2594 3201 3411 3801 794
Size: 1608 192 607 210 390 4890
Fragments arranged by size:
4890 1608 607 390 210 192
BstBI TT'CG_AA
Cuts at: 4561 4762 6701 4561
Size: 201 1939 5757
Fragments arranged by size:
5757 1939 201
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7897
BstYI r'GATC_y
Cuts at: 1972 4543 5227 5594 5979 5996 7448 7534 7546
Size: 2571 684 367 385 17 1452 86 12
Cuts at: 7546 7892 1972
Size: 346 1977
Fragments arranged by size:
2571 1977 1452 684 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2743 3556 3790 3985 513
Size: 552 1678 813 234 195 4425
Fragments arranged by size:
4425 1678 813 552 234 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3915 487
Size: 683 56 2689 4469
Fragments arranged by size:
4469 2689 683 56
BtsI GCAGTG_nn'
Cuts at: 2494 3112 3499 3712 4759 5413 6207 6227 2494
Size: 618 387 213 1047 654 794 20 4164
Fragments arranged by size:
4164 1047 794 654 618 387 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7862
DraI TTT'AAA
Cuts at: 2305 2610 5034 5201 2305
Size: 305 2424 167 5001
Fragments arranged by size:
5001 2424 305 167
DraIII CAC_nnn'GTG
Cuts at: 2402 2402
Size: 7897
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6904 809
Size: 507 5588 1802
Fragments arranged by size:
5588 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5056 5344 6233
Size: 54 1425 50 275 18 3226 288 889
Cuts at: 6233 7862 8
Size: 1629 43
Fragments arranged by size:
3226 1629 1425 889 288 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5344 1537
Size: 275 3532 4090
Fragments arranged by size:
4090 3532 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 3621 4047 4701 5827 1353
Size: 878 1390 426 654 1126 3423
Fragments arranged by size:
3423 1390 1126 878 654 426
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6484 6858 7004 387
Size: 1064 138 4895 374 146 1280
Fragments arranged by size:
4895 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2261 2261
Size: 7897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2874 3066 4267 5960 6557 7344 7612
Size: 393 1242 192 1201 1693 597 787 268
Cuts at: 7612 1239
Size: 1524
Fragments arranged by size:
1693 1524 1242 1201 787 597 393 268
192
EcoNI CCTnn'n_nnAGG
Cuts at: 4614 4614
Size: 7897
EcoO109I rG'GnC_Cy
Cuts at: 2424 3172 3382 2424
Size: 748 210 6939
Fragments arranged by size:
6939 748 210
EcoRI G'AATT_C
Cuts at: 2649 5206 5584 2649
Size: 2557 378 4962
Fragments arranged by size:
4962 2557 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4033 4033
Size: 7897
FspI TGC'GCA
Cuts at: 6403 6403
Size: 7897
HaeII r_GCGC'y
Cuts at: 1678 7044 1678
Size: 5366 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 3855 3887 4190
Size: 32 135 32 880 32 1371 32 303
Cuts at: 4190 4222 4999 5031 6538 6570 6612 6644 1373
Size: 32 777 32 1507 32 42 32 2626
Fragments arranged by size:
2626 1507 1371 880 777 303 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5241 5487 669
Size: 988 281 3303 246 3079
Fragments arranged by size:
3303 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3119 4583 4881 4898 748
Size: 2371 1464 298 17 3747
Fragments arranged by size:
3747 2371 1464 298 17
HpaI GTT'AAC
Cuts at: 5487 5487
Size: 7897
KpnI G_GTAC'C
Cuts at: 5222 5222
Size: 7897
MfeI C'AATT_G
Cuts at: 5496 5496
Size: 7897
MluI A'CGCG_T
Cuts at: 7773 7773
Size: 7897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7011 7195 1305
Size: 5706 184 2007
Fragments arranged by size:
5706 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5058 10
Size: 54 1425 343 3226 2849
Fragments arranged by size:
3226 2849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3529 5855 6214 6373 518
Size: 648 2363 2326 359 159 2042
Fragments arranged by size:
2363 2326 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4164 4460 5313 5990 7138 7383 1606
Size: 2558 296 853 677 1148 245 2120
Fragments arranged by size:
2558 2120 1148 853 677 296 245
NcoI C'CATG_G
Cuts at: 513 1065 3985 513
Size: 552 2920 4425
Fragments arranged by size:
4425 2920 552
NdeI CA'TA_TG
Cuts at: 387 2380 3091 4624 387
Size: 1993 711 1533 3660
Fragments arranged by size:
3660 1993 1533 711
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7897
NotI GC'GGCC_GC
Cuts at: 5344 5344
Size: 7897
NsiI A_TGCA'T
Cuts at: 2766 2982 3402 3579 2766
Size: 216 420 177 7084
Fragments arranged by size:
7084 420 216 177
NspI r_CATG'y
Cuts at: 1132 1229 3900 4326 4570 4858 5083 5182 5255
Size: 97 2671 426 244 288 225 99 73
Cuts at: 5255 6800 1132
Size: 1545 2229
Fragments arranged by size:
2671 2229 1545 426 288 244 225 99
97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7897
PciI A'CATG_T
Cuts at: 4322 4854 6796 4322
Size: 532 1942 5423
Fragments arranged by size:
5423 1942 532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2744 1108
Size: 1636 6261
PfoI T'CCnGG_A
Cuts at: 3332 3332
Size: 7897
PmeI GTTT'AAAC
Cuts at: 5201 5201
Size: 7897
PmlI CAC'GTG
Cuts at: 2199 2199
Size: 7897
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3675 3707 5966 5998 7479 7511 3675
Size: 32 2259 32 1481 32 4061
Fragments arranged by size:
4061 2259 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2424 2424
Size: 7897
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7897
PsiI TTA'TAA
Cuts at: 5467 5467
Size: 7897
PspOMI G'GGCC_C
Cuts at: 3172 3382 3172
Size: 210 7687
PstI C_TGCA'G
Cuts at: 830 2897 3494 4078 5249 830
Size: 2067 597 584 1171 3478
Fragments arranged by size:
3478 2067 1171 597 584
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7897
PvuII CAG'CTG
Cuts at: 4164 4460 4164
Size: 296 7601
SacI G_AGCT'C
Cuts at: 2263 2263
Size: 7897
SalI G'TCGA_C
Cuts at: 1936 5239 1936
Size: 3303 4594
SanDI GG'GwC_CC
Cuts at: 2424 2424
Size: 7897
SbfI CC_TGCA'GG
Cuts at: 5249 5249
Size: 7897
ScaI AGT'ACT
Cuts at: 6145 7857 6145
Size: 1712 6185
SfcI C'TryA_G
Cuts at: 826 1045 2893 3490 4074 5245 6380 7061 7252
Size: 219 1848 597 584 1171 1135 681 191
Cuts at: 7252 826
Size: 1471
Fragments arranged by size:
1848 1471 1171 1135 681 597 584 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7897
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7897
SmaI CCC'GGG
Cuts at: 1872 5224 1872
Size: 3352 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4371 4932 6001 6902 7164 820
Size: 197 926 2428 561 1069 901 262 1553
Fragments arranged by size:
2428 1553 1069 926 901 561 262 197
SnaBI TAC'GTA
Cuts at: 493 5688 493
Size: 5195 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7897
SphI G_CATG'C
Cuts at: 1132 4570 5083 5182 5255 1132
Size: 3438 513 99 73 3774
Fragments arranged by size:
3774 3438 513 99 73
SspI AAT'ATT
Cuts at: 5 52 5821 5
Size: 47 5769 2081
Fragments arranged by size:
5769 2081 47
StuI AGG'CCT
Cuts at: 1517 3428 1517
Size: 1911 5986
StyI C'CwwG_G
Cuts at: 513 1065 2037 2265 3985 5336 513
Size: 552 972 228 1720 1351 3074
Fragments arranged by size:
3074 1720 1351 972 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5924 5941 6094 6279 6724 325
Size: 1255 4344 17 153 185 445 1498
Fragments arranged by size:
4344 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2720 3290 4166
Size: 274 80 33 51 1443 743 570 876
Cuts at: 4166 4424 5129 6143 7855 96
Size: 258 705 1014 1712 138
Fragments arranged by size:
1712 1443 1014 876 743 705 570 274
258 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7857 7889 7857
Size: 32 7865
Tth111I GACn'n_nGTC
Cuts at: 1316 4385 1316
Size: 3069 4828
XbaI T'CTAG_A
Cuts at: 3775 5233 3775
Size: 1458 6439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3176 3386 3515 4349 3176
Size: 210 129 834 6724
Fragments arranged by size:
6724 834 210 129
XhoI C'TCGA_G
Cuts at: 1943 4371 1943
Size: 2428 5469
XmaI C'CCGG_G
Cuts at: 1870 5222 1870
Size: 3352 4545
XmnI GAAnn'nnTTC
Cuts at: 3690 6026 3690
Size: 2336 5561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5705 276
Size: 53 83 186 936 4171 2468
Fragments arranged by size:
4171 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI