Restriction map for
pFN21AB7303
(Circular) (Six-base) MAPSORT of: pFN21AB7303.seq Check: 3529 from: 1 to: 8569
pFN21AB7303 8569 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2785 3750 4497 2602
Size: 183 965 747 6674
Fragments arranged by size:
6674 965 747 183
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2067 5705 6379 278
Size: 53 83 186 936 531 3638 674 2468
Fragments arranged by size:
3638 2468 936 674 531 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5912 1937
Size: 3975 4594
Acc65I G'GTAC_C
Cuts at: 2051 5466 5890 2051
Size: 3415 424 4730
Fragments arranged by size:
4730 3415 424
AclI AA'CG_TT
Cuts at: 1563 6696 7069 1563
Size: 5133 373 3063
Fragments arranged by size:
5133 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2827 3073 5026 6632 8016 8284 1632
Size: 1195 246 1953 1606 1384 268 1917
Fragments arranged by size:
1953 1917 1606 1384 1195 268 246
AfeI AGC'GCT
Cuts at: 2744 2744
Size: 8569
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8372
AflIII A'CryG_T
Cuts at: 1203 2306 2419 2589 2649 4902 7468 8445 1203
Size: 1103 113 170 60 2253 2566 977 1327
Fragments arranged by size:
2566 2253 1327 1103 977 170 113 60
AgeI A'CCGG_T
Cuts at: 4513 7400 4513
Size: 2887 5682
AhdI GACnn_n'nnGTC
Cuts at: 4762 7298 4762
Size: 2536 6033
AleI CACnn'nnGTG
Cuts at: 1166 2249 2648 2966 4383 4514 1166
Size: 1083 399 318 1417 131 5221
Fragments arranged by size:
5221 1417 1083 399 318 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 3510 4401 4505 5426 1184
Size: 1023 1303 891 104 921 4327
Fragments arranged by size:
4327 1303 1023 921 891 104
AlwNI CAG_nnn'CTG
Cuts at: 2209 3655 4423 6312 7884 2209
Size: 1446 768 1889 1572 2894
Fragments arranged by size:
2894 1889 1572 1446 768
ApaI G_GGCC'C
Cuts at: 2518 2599 2873 3117 3167 3330 3511 4077 4196
Size: 81 274 244 50 163 181 566 119
Cuts at: 4196 4338 5857 2518
Size: 142 1519 5230
Fragments arranged by size:
5230 1519 566 274 244 181 163 142
119 81 50
ApaLI G'TGCA_C
Cuts at: 3414 3527 4376 5411 6626 7782 3414
Size: 113 849 1035 1215 1156 4201
Fragments arranged by size:
4201 1215 1156 1035 849 113
ApoI r'AATT_y
Cuts at: 1493 2987 5878 6104 6256 8365 1493
Size: 1494 2891 226 152 2109 1697
Fragments arranged by size:
2891 2109 1697 1494 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7123 160
Size: 872 6091 1606
Fragments arranged by size:
6091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8569
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2196 2547 2628 2694 2866 3116
Size: 73 72 181 351 81 66 172 250
Cuts at: 3116 3504 3941 3975 4935 5190 5216 5506 5720
Size: 388 437 34 960 255 26 290 214
Cuts at: 5720 5822 5894 1870
Size: 102 72 4545
Fragments arranged by size:
4545 960 437 388 351 290 255 250
214 181 172 102 81 73 72 72
66 34 26
BamHI G'GATC_C
Cuts at: 5899 6266 5899
Size: 367 8202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2243 2336 2447 2827
Size: 325 217 627 264 192 93 111 380
Cuts at: 2827 3917 4385 4566 4637 4711 5167 5466 5639
Size: 1090 468 181 71 74 456 299 173
Cuts at: 5639 5890 7345 618
Size: 251 1455 1842
Fragments arranged by size:
1842 1455 1090 627 468 456 380 325
299 264 251 217 192 181 173 111
93 74 71
BanII G_rGCy'C
Cuts at: 1391 2518 2599 2873 3117 3133 3167 3330 3511
Size: 1127 81 274 244 16 34 163 181
Cuts at: 3511 4077 4196 4338 4824 5507 5622 5857 5888
Size: 566 119 142 486 683 115 235 31
Cuts at: 5888 1391
Size: 4072
Fragments arranged by size:
4072 1127 683 566 486 274 244 235
181 163 142 119 115 81 34 31
16
BbeI G_GCGC'C
Cuts at: 2831 4715 5171 5643 2831
Size: 1884 456 472 5757
Fragments arranged by size:
5757 1884 472 456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2992 3926 4669 4971 928
Size: 971 1093 934 743 302 4526
Fragments arranged by size:
4526 1093 971 934 743 302
BbvCI CC'TCA_GC
Cuts at: 3445 3445
Size: 8569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6760 6794 1903
Size: 34 4823 34 3678
Fragments arranged by size:
4823 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6461 7671 6461
Size: 1210 7359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8569
BfrBI ATG'CAT
Cuts at: 3491 3491
Size: 8569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2019 2877 3287
Size: 107 122 71 631 605 347 858 410
Cuts at: 3287 3392 4709 4784 5288 5617 5861 7180 136
Size: 105 1317 75 504 329 244 1319 1525
Fragments arranged by size:
1525 1319 1317 858 631 605 504 410
347 329 244 122 107 105 75 71
BglII A'GATC_T
Cuts at: 8564 8564
Size: 8569
BlpI GC'TnA_GC
Cuts at: 3347 3656 4251 4884 5986 3347
Size: 309 595 633 1102 5930
Fragments arranged by size:
5930 1102 633 595 309
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2091 2246 2376 2450 2518 2599 2703
Size: 291 10 155 130 74 68 81 104
Cuts at: 2703 2873 3117 3167 3330 3418 3511 3531 3758
Size: 170 244 50 163 88 93 20 227
Cuts at: 3758 3920 4062 4077 4196 4327 4338 4380 4569
Size: 162 142 15 119 131 11 42 189
Cuts at: 4569 4780 5415 5857 6630 7786 1790
Size: 211 635 442 773 1156 2573
Fragments arranged by size:
2573 1156 773 635 442 291 244 227
211 189 170 163 162 155 142 131
130 119 104 93 88 81 74 68
50 42 20 15 11 10
BmgBI CAC'GTC
Cuts at: 1318 2958 2998 3583 4450 4846 5250 1318
Size: 1640 40 585 867 396 404 4637
Fragments arranged by size:
4637 1640 867 585 404 396 40
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 3988 4464
Size: 416 53 83 399 24 651 1920 476
Cuts at: 4464 5061 6300 7258 442
Size: 597 1239 958 1753
Fragments arranged by size:
1920 1753 1239 958 651 597 476 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8569
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4975 4975
Size: 8569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2200 2291 2426 2552 2836 3014 3305
Size: 950 11 91 135 126 284 178 291
Cuts at: 3305 3374 3686 4970 5477 7229 1239
Size: 69 312 1284 507 1752 2579
Fragments arranged by size:
2579 1752 1284 950 507 312 291 284
178 135 126 91 69 11
Bpu10I CC'TnA_GC
Cuts at: 3445 3879 3445
Size: 434 8135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 6694 7559 7857 2768
Size: 3926 865 298 3480
Fragments arranged by size:
3926 3480 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2313 3623 4016 7232 882
Size: 617 814 1310 393 3216 2219
Fragments arranged by size:
3216 2219 1310 814 617 393
BsaAI yAC'GTr
Cuts at: 493 1204 2092 2309 2592 2650 2749 6360 493
Size: 711 888 217 283 58 99 3611 2702
Fragments arranged by size:
3611 2702 888 711 283 217 99 58
BsaBI GATnn'nnATC
Cuts at: 3186 8563 3186
Size: 5377 3192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2456 2718 2828
Size: 53 83 186 936 531 392 262 110
Cuts at: 2828 3021 4674 4712 5168 5541 5640 5702 6376
Size: 193 1653 38 456 373 99 62 674
Cuts at: 6376 6758 7393 275
Size: 382 635 1451
Fragments arranged by size:
1653 1451 936 674 635 531 456 392
382 373 262 193 186 110 99 83
62 53 38
BsaWI w'CCGG_w
Cuts at: 4513 7002 7400 7674 7821 8232 4513
Size: 2489 398 274 147 411 4850
Fragments arranged by size:
4850 2489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3659 3689 3955 3985 5450
Size: 30 165 30 2256 30 266 30 1465
Cuts at: 5450 5480 1178
Size: 30 4267
Fragments arranged by size:
4267 2256 1465 266 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3371 3683 4700 5582 1186
Size: 2185 312 1017 882 4173
Fragments arranged by size:
4173 2185 1017 882 312
BseYI C'CCAG_C
Cuts at: 2598 2635 2981 3793 4048 4197 5387 7772 8268
Size: 37 346 812 255 149 1190 2385 496
Cuts at: 8268 2598
Size: 2899
Fragments arranged by size:
2899 2385 1190 812 496 346 255 149
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 2414 2513 2771 2813 3659 3722
Size: 573 576 102 99 258 42 846 63
Cuts at: 3722 4424 4736 4871 5024 1163
Size: 702 312 135 153 4708
Fragments arranged by size:
4708 846 702 576 573 312 258 153
135 102 99 63 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2044 2877 3887 3925 5677 6019
Size: 275 186 43 833 1010 38 1752 342
Cuts at: 6019 6780 7808 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1752 1028 1010 833 761 342 275
186 43 38
BsiHKAI G_wGCw'C
Cuts at: 2577 2865 2997 3418 3531 3769 4380 4824 5247
Size: 288 132 421 113 238 611 444 423
Cuts at: 5247 5415 5829 6630 6715 7786 2577
Size: 168 414 801 85 1071 3360
Fragments arranged by size:
3360 1071 801 611 444 423 421 414
288 238 168 132 113 85
BsmI GAATG_Cn'
Cuts at: 6080 6173 6080
Size: 93 8476
BsmBI CGTCTCn'nnnn_
Cuts at: 4391 5351 4391
Size: 960 7609
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2091 2246 2376 2450 2518 2577
Size: 399 291 10 155 130 74 68 59
Cuts at: 2577 2599 2703 2865 2873 2997 3117 3133 3167
Size: 22 104 162 8 124 120 16 34
Cuts at: 3167 3330 3418 3511 3531 3758 3769 3920 4062
Size: 163 88 93 20 227 11 151 142
Cuts at: 4062 4077 4196 4327 4338 4380 4569 4780 4824
Size: 15 119 131 11 42 189 211 44
Cuts at: 4824 5247 5415 5507 5622 5829 5857 5888 6630
Size: 423 168 92 115 207 28 31 742
Cuts at: 6630 6715 7786 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 291 227 211
207 189 168 163 162 155 151 142
131 130 124 120 119 115 104 93
92 88 85 74 68 59 44 42
34 31 28 22 20 16 15 11
11 10 8
BspEI T'CCGG_A
Cuts at: 8232 8232
Size: 8569
BspHI T'CATG_A
Cuts at: 4239 6458 8188 4239
Size: 2219 1730 4620
Fragments arranged by size:
4620 2219 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 2490 2602 2785 3750 4044 4497 4758
Size: 1487 159 112 183 965 294 453 261
Cuts at: 4758 5664 5924 844
Size: 906 260 3489
Fragments arranged by size:
3489 1487 965 906 453 294 261 260
183 159 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 2287 3807 3847 3883 6456 1394
Size: 429 452 12 1520 40 36 2573 3507
Fragments arranged by size:
3507 2573 1520 452 429 40 36 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4245 7064 7238 65
Size: 1183 2997 2819 174 1396
Fragments arranged by size:
2997 2819 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 2876 3402 3886 3920 4513 5463
Size: 110 370 696 526 484 34 593 950
Cuts at: 5463 7213 7400 1700
Size: 1750 187 2869
Fragments arranged by size:
2869 1750 950 696 593 526 484 370
187 110 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8569
BssSI C'ACGA_G
Cuts at: 6629 7641 6629
Size: 1012 7557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8569
BstBI TT'CG_AA
Cuts at: 7373 7373
Size: 8569
BstEII G'GTnAC_C
Cuts at: 1184 4135 5707 1184
Size: 2951 1572 4046
Fragments arranged by size:
4046 2951 1572
BstXI CCAn_nnnn'nTGG
Cuts at: 2348 2677 4111 2348
Size: 329 1434 6806
Fragments arranged by size:
6806 1434 329
BstYI r'GATC_y
Cuts at: 1972 5451 5899 6266 6651 6668 8120 8206 8218
Size: 3479 448 367 385 17 1452 86 12
Cuts at: 8218 8564 1972
Size: 346 1977
Fragments arranged by size:
3479 1977 1452 448 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2382 3264 2382
Size: 882 7687
BtgI C'CryG_G
Cuts at: 513 1065 2004 2044 2347 2434 2676 2764 2832
Size: 552 939 40 303 87 242 88 68
Cuts at: 2832 2961 3057 3366 4069 4164 5084 5623 5644
Size: 129 96 309 703 95 920 539 21
Cuts at: 5644 5865 513
Size: 221 3217
Fragments arranged by size:
3217 939 920 703 552 539 309 303
242 221 129 96 95 88 87 68
40 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3484 487
Size: 683 56 2258 5572
Fragments arranged by size:
5572 2258 683 56
BtsI GCAGTG_nn'
Cuts at: 2264 3680 3934 6085 6879 6899 2264
Size: 1416 254 2151 794 20 3934
Fragments arranged by size:
3934 2151 1416 794 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8534
DraI TTT'AAA
Cuts at: 5873 5873
Size: 8569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7576 809
Size: 507 6260 1802
Fragments arranged by size:
6260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2367 2874
Size: 54 1425 50 275 18 423 114 507
Cuts at: 2874 2923 3294 3363 3384 3884 3922 4523 4554
Size: 49 371 69 21 500 38 601 31
Cuts at: 4554 4890 5609 5674 6016 6905 8534 8
Size: 336 719 65 342 889 1629 43
Fragments arranged by size:
1629 1425 889 719 601 507 500 423
371 342 336 275 114 69 65 54
50 49 43 38 31 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2874 3884 3922 5674 6016 1537
Size: 275 1062 1010 38 1752 342 4090
Fragments arranged by size:
4090 1752 1062 1010 342 275 38
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 3089 6499 1353
Size: 1038 698 3410 3423
Fragments arranged by size:
3423 3410 1038 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 4982 5092 7156 7530 7676
Size: 1064 138 558 2835 110 2064 374 146
Cuts at: 7676 387
Size: 1280
Fragments arranged by size:
2835 2064 1280 1064 558 374 146 138
110
EcoICRI GAG'CTC
Cuts at: 4822 4822
Size: 8569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2200 2291 2426 2552 2827 2836
Size: 393 557 11 91 135 126 275 9
Cuts at: 2836 3014 3073 3305 3374 3686 4970 5026 5477
Size: 178 59 232 69 312 1284 56 451
Cuts at: 5477 6632 7229 8016 8284 1239
Size: 1155 597 787 268 1524
Fragments arranged by size:
1524 1284 1155 787 597 557 451 393
312 275 268 232 178 135 126 91
69 59 56 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2171 2463 3356 5018 2171
Size: 292 893 1662 5722
Fragments arranged by size:
5722 1662 893 292
EcoO109I rG'GnC_Cy
Cuts at: 2119 2353 2479 2514 2515 3034 3140 3163 3164
Size: 234 126 35 1 519 106 23 1
Cuts at: 3164 3327 3508 3711 4074 4193 4702 4723 4939
Size: 163 181 203 363 119 509 21 216
Cuts at: 4939 5280 5289 5384 5767 5776 2119
Size: 341 9 95 383 9 4912
Fragments arranged by size:
4912 519 509 383 363 341 234 216
203 181 163 126 119 106 95 35
23 21 9 9 1 1
EcoRI G'AATT_C
Cuts at: 2987 5878 6256 2987
Size: 2891 378 5300
Fragments arranged by size:
5300 2891 378
FspI TGC'GCA
Cuts at: 2684 7075 2684
Size: 4391 4178
FspAI rTGC'GCAy
Cuts at: 2684 2684
Size: 8569
HaeII r_GCGC'y
Cuts at: 1678 2297 2746 2831 4715 5031 5171 5576 5599
Size: 619 449 85 1884 316 140 405 23
Cuts at: 5599 5643 7716 1678
Size: 44 2073 2531
Fragments arranged by size:
2531 2073 1884 619 449 405 316 140
85 44 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3955 3987 4450 4482 4748
Size: 32 135 32 2383 32 463 32 266
Cuts at: 4748 4780 5184 5216 5671 5703 7210 7242 7284
Size: 32 404 32 455 32 1507 32 42
Cuts at: 7284 7316 1373
Size: 32 2626
Fragments arranged by size:
2626 2383 1507 463 455 404 266 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3307 5913 6159 669
Size: 988 281 1369 2606 246 3079
Fragments arranged by size:
3079 2606 1369 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8569
HpaI GTT'AAC
Cuts at: 6159 6159
Size: 8569
KasI G'GCGC_C
Cuts at: 2827 4711 5167 5639 2827
Size: 1884 456 472 5757
Fragments arranged by size:
5757 1884 472 456
KpnI G_GTAC'C
Cuts at: 2055 5470 5894 2055
Size: 3415 424 4730
Fragments arranged by size:
4730 3415 424
MfeI C'AATT_G
Cuts at: 6168 6168
Size: 8569
MluI A'CGCG_T
Cuts at: 2419 8445 2419
Size: 6026 2543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 7683 7867 1305
Size: 3441 2937 184 2007
Fragments arranged by size:
3441 2937 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 2925 3296 3365 3386
Size: 54 1425 343 423 670 371 69 21
Cuts at: 3386 4556 10
Size: 1170 4023
Fragments arranged by size:
4023 1425 1170 670 423 371 343 69
54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2249 2648 2675 2966 3492 4109
Size: 648 1071 12 399 27 291 526 617
Cuts at: 4109 4163 4373 4383 4470 4514 6527 6886 7045
Size: 54 210 10 87 44 2013 359 159
Cuts at: 7045 518
Size: 2042
Fragments arranged by size:
2042 2013 1071 648 617 526 399 359
291 210 159 87 54 44 27 12
10
MspA1I CmG'CkG
Cuts at: 1606 2209 2847 3017 3043 3065 3346 3643 4201
Size: 603 638 170 26 22 281 297 558
Cuts at: 4201 4216 4737 4810 5046 5086 5391 5406 5646
Size: 15 521 73 236 40 305 15 240
Cuts at: 5646 5985 6662 7810 8055 1606
Size: 339 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 603 558 521 339
305 297 281 245 240 236 170 73
40 26 22 15 15
NaeI GCC'GGC
Cuts at: 2182 2878 3404 2182
Size: 696 526 7347
Fragments arranged by size:
7347 696 526
NarI GG'CG_CC
Cuts at: 2828 4712 5168 5640 2828
Size: 1884 456 472 5757
Fragments arranged by size:
5757 1884 472 456
NcoI C'CATG_G
Cuts at: 513 1065 2004 2676 2832 3366 4069 513
Size: 552 939 672 156 534 703 5013
Fragments arranged by size:
5013 939 703 672 552 534 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8569
NgoMIV G'CCGG_C
Cuts at: 2180 2876 3402 2180
Size: 696 526 7347
Fragments arranged by size:
7347 696 526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8569
NotI GC'GGCC_GC
Cuts at: 6016 6016
Size: 8569
NsiI A_TGCA'T
Cuts at: 3493 3493
Size: 8569
NspI r_CATG'y
Cuts at: 1132 1229 2188 3491 3495 4906 5927 7472 1132
Size: 97 959 1303 4 1411 1021 1545 2229
Fragments arranged by size:
2229 1545 1411 1303 1021 959 97 4
PasI CC'CwG_GG
Cuts at: 1348 2081 3511 3562 3697 5305 5329 1348
Size: 733 1430 51 135 1608 24 4588
Fragments arranged by size:
4588 1608 1430 733 135 51 24
PciI A'CATG_T
Cuts at: 4902 7468 4902
Size: 2566 6003
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 3457 4813 1108
Size: 1101 1248 1356 4864
Fragments arranged by size:
4864 1356 1248 1101
PfoI T'CCnGG_A
Cuts at: 2166 4680 5202 5454 2166
Size: 2514 522 252 5281
Fragments arranged by size:
5281 2514 522 252
PmeI GTTT'AAAC
Cuts at: 5873 5873
Size: 8569
PmlI CAC'GTG
Cuts at: 2092 2309 2592 2650 2092
Size: 217 283 58 8011
Fragments arranged by size:
8011 283 217 58
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6638 6670 8151 8183 6638
Size: 32 1481 32 7024
Fragments arranged by size:
7024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2353 2479 3140 3711 4723 5384 5767 5776
Size: 234 126 661 571 1012 661 383 9
Cuts at: 5776 2119
Size: 4912
Fragments arranged by size:
4912 1012 661 661 571 383 234 126
9
PshAI GACnn'nnGTC
Cuts at: 1613 2217 1613
Size: 604 7965
PsiI TTA'TAA
Cuts at: 6139 6139
Size: 8569
PspOMI G'GGCC_C
Cuts at: 2514 2595 2869 3113 3163 3326 3507 4073 4192
Size: 81 274 244 50 163 181 566 119
Cuts at: 4192 4334 5853 2514
Size: 142 1519 5230
Fragments arranged by size:
5230 1519 566 274 244 181 163 142
119 81 50
PspXI vC'TCGA_Gb
Cuts at: 3941 3941
Size: 8569
PstI C_TGCA'G
Cuts at: 830 3693 4275 4736 5921 830
Size: 2863 582 461 1185 3478
Fragments arranged by size:
3478 2863 1185 582 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8569
PvuII CAG'CTG
Cuts at: 3017 3043 3643 4201 4216 4810 5391 3017
Size: 26 600 558 15 594 581 6195
Fragments arranged by size:
6195 600 594 581 558 26 15
SacI G_AGCT'C
Cuts at: 4824 4824
Size: 8569
SacII CC_GC'GG
Cuts at: 5087 5647 5087
Size: 560 8009
SalI G'TCGA_C
Cuts at: 1936 5911 1936
Size: 3975 4594
SanDI GG'GwC_CC
Cuts at: 5384 5767 5384
Size: 383 8186
SbfI CC_TGCA'GG
Cuts at: 5921 5921
Size: 8569
ScaI AGT'ACT
Cuts at: 6817 8529 6817
Size: 1712 6857
SfcI C'TryA_G
Cuts at: 826 1045 3689 4271 4732 5917 7052 7733 7924
Size: 219 2644 582 461 1185 1135 681 191
Cuts at: 7924 826
Size: 1471
Fragments arranged by size:
2644 1471 1185 1135 681 582 461 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3392 5288 5861 3392
Size: 1896 573 6100
Fragments arranged by size:
6100 1896 573
SfoI GGC'GCC
Cuts at: 2829 4713 5169 5641 2829
Size: 1884 456 472 5757
Fragments arranged by size:
5757 1884 472 456
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8569
SgrAI Cr'CCGG_yG
Cuts at: 1700 4513 1700
Size: 2813 5756
SmaI CCC'GGG
Cuts at: 1872 2198 2630 3506 3977 4937 5722 5896 1872
Size: 326 432 876 471 960 785 174 4545
Fragments arranged by size:
4545 960 876 785 471 432 326 174
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2547 2783 3941 6673 7574 7836
Size: 197 926 604 236 1158 2732 901 262
Cuts at: 7836 820
Size: 1553
Fragments arranged by size:
2732 1553 1158 926 901 604 262 236
197
SnaBI TAC'GTA
Cuts at: 493 6360 493
Size: 5867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8569
SphI G_CATG'C
Cuts at: 1132 2188 3491 5927 1132
Size: 1056 1303 2436 3774
Fragments arranged by size:
3774 2436 1303 1056
SrfI GCCC'GGGC
Cuts at: 3506 3506
Size: 8569
SspI AAT'ATT
Cuts at: 5 52 6493 5
Size: 47 6441 2081
Fragments arranged by size:
6441 2081 47
StuI AGG'CCT
Cuts at: 1517 3269 4856 1517
Size: 1752 1587 5230
Fragments arranged by size:
5230 1752 1587
StyI C'CwwG_G
Cuts at: 513 1065 2004 2676 2832 3366 4069 6008 513
Size: 552 939 672 156 534 703 1939 3074
Fragments arranged by size:
3074 1939 939 703 672 552 534 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 2606 3150 3807 4373 5606 6596
Size: 1255 774 252 544 657 566 1233 990
Cuts at: 6596 6613 6766 6951 7396 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1233 990 774 657 566 544
445 252 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6815 8527 96
Size: 274 80 33 51 1443 4838 1712 138
Fragments arranged by size:
4838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2298 2330 3659 3691 8529 8561 2298
Size: 32 1329 32 4838 32 2306
Fragments arranged by size:
4838 2306 1329 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5540 1316
Size: 4224 4345
XbaI T'CTAG_A
Cuts at: 5905 5905
Size: 8569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 2266 2918 3542 2236
Size: 30 652 624 7263
Fragments arranged by size:
7263 652 624 30
XhoI C'TCGA_G
Cuts at: 1943 2547 3941 1943
Size: 604 1394 6571
Fragments arranged by size:
6571 1394 604
XmaI C'CCGG_G
Cuts at: 1870 2196 2628 3504 3975 4935 5720 5894 1870
Size: 326 432 876 471 960 785 174 4545
Fragments arranged by size:
4545 960 876 785 471 432 326 174
XmnI GAAnn'nnTTC
Cuts at: 6698 6698
Size: 8569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2065 5703 6377 276
Size: 53 83 186 936 531 3638 674 2468
Fragments arranged by size:
3638 2468 936 674 531 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoRV
FalI FseI NruI PacI PsrI RsrII SapI SexAI
SwaI