Restriction map for
pFN21AB7301
(Circular) (Six-base) MAPSORT of: pFN21AB7301.seq Check: 8307 from: 1 to: 7258
pFN21AB7301 7258 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3260 5068 278
Size: 53 83 186 936 1724 1808 2468
Fragments arranged by size:
2468 1808 1724 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3318 4601 1937
Size: 1381 1283 4594
Fragments arranged by size:
4594 1381 1283
Acc65I G'GTAC_C
Cuts at: 4579 4579
Size: 7258
AclI AA'CG_TT
Cuts at: 1563 5385 5758 1563
Size: 3822 373 3063
Fragments arranged by size:
3822 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2784 2964 3147 3252 3317 3984 4044 5321
Size: 1152 180 183 105 65 667 60 1277
Cuts at: 5321 6705 6973 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1277 1152 667 268 183 180
105 65 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7061
AflIII A'CryG_T
Cuts at: 1203 3044 6157 7134 1203
Size: 1841 3113 977 1327
Fragments arranged by size:
3113 1841 1327 977
AgeI A'CCGG_T
Cuts at: 6089 6089
Size: 7258
AhdI GACnn_n'nnGTC
Cuts at: 3256 5987 3256
Size: 2731 4527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7258
AlwNI CAG_nnn'CTG
Cuts at: 2234 4204 5001 6573 2234
Size: 1970 797 1572 2919
Fragments arranged by size:
2919 1970 1572 797
ApaLI G'TGCA_C
Cuts at: 5315 6471 5315
Size: 1156 6102
ApoI r'AATT_y
Cuts at: 1493 2438 2483 3167 3348 3374 3466 4567 4793
Size: 945 45 684 181 26 92 1101 226
Cuts at: 4793 4945 7054 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1101 945 684 226 181 152
92 45 26
AseI AT'TA_AT
Cuts at: 160 1032 4247 5812 160
Size: 872 3215 1565 1606
Fragments arranged by size:
3215 1606 1565 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7258
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 4583 1870
Size: 73 119 2521 4545
Fragments arranged by size:
4545 2521 119 73
BamHI G'GATC_C
Cuts at: 4588 4955 4588
Size: 367 6891
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2175 2576 4579 6034 618
Size: 325 217 627 388 401 2003 1455 1842
Fragments arranged by size:
2003 1842 1455 627 401 388 325 217
BanII G_rGCy'C
Cuts at: 1391 4577 1391
Size: 3186 4072
BbeI G_GCGC'C
Cuts at: 2179 2179
Size: 7258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5449 5483 1903
Size: 34 3512 34 3678
Fragments arranged by size:
3678 3512 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5150 6360 5150
Size: 1210 6048
BclI T'GATC_A
Cuts at: 1548 3547 1548
Size: 1999 5259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5869 136
Size: 107 122 71 631 605 4197 1525
Fragments arranged by size:
4197 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3400 7253 3400
Size: 3853 3405
BlpI GC'TnA_GC
Cuts at: 4675 4675
Size: 7258
Bme1580I G_kGCm'C
Cuts at: 1790 5319 6475 1790
Size: 3529 1156 2573
Fragments arranged by size:
3529 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3047 1318
Size: 1729 5529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3082 4989 5947
Size: 416 53 83 399 24 1665 1907 958
Cuts at: 5947 442
Size: 1753
Fragments arranged by size:
1907 1753 1665 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 4071 5918 1239
Size: 1502 1330 1847 2579
Fragments arranged by size:
2579 1847 1502 1330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4394 5383 6248 6546 4394
Size: 989 865 298 5106
Fragments arranged by size:
5106 989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5921 882
Size: 617 4422 2219
Fragments arranged by size:
4422 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5049 493
Size: 711 3845 2702
Fragments arranged by size:
3845 2702 711
BsaBI GATnn'nnATC
Cuts at: 2241 7252 2241
Size: 5011 2247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2176 3257 5065 5447
Size: 53 83 186 936 643 1081 1808 382
Cuts at: 5447 6082 275
Size: 635 1451
Fragments arranged by size:
1808 1451 1081 936 643 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3331 5691 6089 6363 6510 6921 3331
Size: 2360 398 274 147 411 3668
Fragments arranged by size:
3668 2360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3672 3702 1178
Size: 30 165 30 2269 30 4734
Fragments arranged by size:
4734 2269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2849 2867 3015 3662 3674 4391 1186
Size: 1663 18 148 647 12 717 4053
Fragments arranged by size:
4053 1663 717 647 148 18 12
BseYI C'CCAG_C
Cuts at: 6461 6957 6461
Size: 496 6762
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3867 1163
Size: 573 2131 4554
Fragments arranged by size:
4554 2131 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4708 5469 6497 1540
Size: 275 186 2707 761 1028 2301
Fragments arranged by size:
2707 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4197 5319 5404 6475 4197
Size: 1122 85 1071 4980
Fragments arranged by size:
4980 1122 1071 85
BsmI GAATG_Cn'
Cuts at: 4769 4862 4769
Size: 93 7165
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4197 4577 5319 5404 6475 1391
Size: 399 2407 380 742 85 1071 2174
Fragments arranged by size:
2407 2174 1071 742 399 380 85
BspEI T'CCGG_A
Cuts at: 3331 6921 3331
Size: 3590 3668
BspHI T'CATG_A
Cuts at: 2131 3550 4230 5147 6877 2131
Size: 1419 680 917 1730 2512
Fragments arranged by size:
2512 1730 1419 917 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3754 4613 844
Size: 2910 859 3489
Fragments arranged by size:
3489 2910 859
BsrBI CCG'CTC
Cuts at: 1394 1823 5145 1394
Size: 429 3322 3507
Fragments arranged by size:
3507 3322 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3513 4302 5753 5927 65
Size: 1183 2265 789 1451 174 1396
Fragments arranged by size:
2265 1451 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5902 6089 1700
Size: 110 4092 187 2869
Fragments arranged by size:
4092 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4299 96
Size: 4203 3055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7258
BssSI C'ACGA_G
Cuts at: 3027 5318 6330 3027
Size: 2291 1012 3955
Fragments arranged by size:
3955 2291 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3760 794
Size: 2966 4292
BstBI TT'CG_AA
Cuts at: 6062 6062
Size: 7258
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7258
BstYI r'GATC_y
Cuts at: 1972 3400 4333 4588 4955 5340 5357 6809 6895
Size: 1428 933 255 367 385 17 1452 86
Cuts at: 6895 6907 7253 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1428 933 385 367 346 255
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3444 3444
Size: 7258
BtgI C'CryG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 5611
Fragments arranged by size:
5611 1095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6519
Fragments arranged by size:
6519 683 56
BtsI GCAGTG_nn'
Cuts at: 4774 5568 5588 4774
Size: 794 20 6444
Fragments arranged by size:
6444 794 20
ClaI AT'CG_AT
Cuts at: 1554 2319 1554
Size: 765 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7223
DraI TTT'AAA
Cuts at: 3121 3583 4562 3121
Size: 462 979 5817
Fragments arranged by size:
5817 979 462
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6265 809
Size: 507 4949 1802
Fragments arranged by size:
4949 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4705 5594 7223
Size: 54 1425 50 275 18 2875 889 1629
Cuts at: 7223 8
Size: 43
Fragments arranged by size:
2875 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4705 1537
Size: 275 2893 4090
Fragments arranged by size:
4090 2893 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2545 2620 2826 2844 3186 3585 3639 3651
Size: 1192 75 206 18 342 399 54 12
Cuts at: 3651 3846 3960 3972 5188 1353
Size: 195 114 12 1216 3423
Fragments arranged by size:
3423 1216 1192 399 342 206 195 114
75 54 18 12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5845 6219 6365 387
Size: 1064 138 4256 374 146 1280
Fragments arranged by size:
4256 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 2784 2964 3147 3252 3317 3984
Size: 393 1109 43 180 183 105 65 667
Cuts at: 3984 4044 4071 5321 5918 6705 6973 1239
Size: 60 27 1250 597 787 268 1524
Fragments arranged by size:
1524 1250 1109 787 667 597 393 268
183 180 105 65 60 43 27
EcoO109I rG'GnC_Cy
Cuts at: 2059 2059
Size: 7258
EcoRI G'AATT_C
Cuts at: 4567 4945 4567
Size: 378 6880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4001 4186 4001
Size: 185 7073
FspI TGC'GCA
Cuts at: 5764 5764
Size: 7258
HaeII r_GCGC'y
Cuts at: 1678 2179 6405 1678
Size: 501 4226 2531
Fragments arranged by size:
4226 2531 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 3031 3063 3242
Size: 32 135 32 1178 32 249 32 179
Cuts at: 3242 3265 3274 3297 3440 3472 4067 4099 5899
Size: 23 9 23 143 32 595 32 1800
Cuts at: 5899 5931 5973 6005 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1800 1178 595 249 179 143 135
42 32 32 32 32 32 32 32
32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 4276 4602 4848 669
Size: 988 281 2338 326 246 3079
Fragments arranged by size:
3079 2338 988 326 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7258
HpaI GTT'AAC
Cuts at: 4276 4848 4276
Size: 572 6686
KasI G'GCGC_C
Cuts at: 2175 2175
Size: 7258
KpnI G_GTAC'C
Cuts at: 4583 4583
Size: 7258
MfeI C'AATT_G
Cuts at: 4857 4857
Size: 7258
MluI A'CGCG_T
Cuts at: 7134 7134
Size: 7258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6372 6556 1305
Size: 5067 184 2007
Fragments arranged by size:
5067 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5436
Fragments arranged by size:
5436 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5216 5575 5734 518
Size: 648 4050 359 159 2042
Fragments arranged by size:
4050 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 4674 5351 6499 6744 1606
Size: 432 2636 677 1148 245 2120
Fragments arranged by size:
2636 2120 1148 677 432 245
NarI GG'CG_CC
Cuts at: 2176 2176
Size: 7258
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6706
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7258
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7258
NotI GC'GGCC_GC
Cuts at: 4705 4705
Size: 7258
NspI r_CATG'y
Cuts at: 1132 1229 2999 4616 6161 1132
Size: 97 1770 1617 1545 2229
Fragments arranged by size:
2229 1770 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7258
PciI A'CATG_T
Cuts at: 6157 6157
Size: 7258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7258
PmeI GTTT'AAAC
Cuts at: 4562 4562
Size: 7258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5327 5359 6840 6872 5327
Size: 32 1481 32 5713
Fragments arranged by size:
5713 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2059
Size: 7258
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7258
PsiI TTA'TAA
Cuts at: 4828 4828
Size: 7258
PstI C_TGCA'G
Cuts at: 830 4610 830
Size: 3780 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7258
SalI G'TCGA_C
Cuts at: 1936 4600 1936
Size: 2664 4594
SapI GCTCTTCn'nnn_
Cuts at: 3186 3585 3186
Size: 399 6859
SbfI CC_TGCA'GG
Cuts at: 4610 4610
Size: 7258
ScaI AGT'ACT
Cuts at: 3302 5506 7218 3302
Size: 2204 1712 3342
Fragments arranged by size:
3342 2204 1712
SfcI C'TryA_G
Cuts at: 826 1045 2196 2406 2532 4606 5741 6422 6613
Size: 219 1151 210 126 2074 1135 681 191
Cuts at: 6613 826
Size: 1471
Fragments arranged by size:
2074 1471 1151 1135 681 219 210 191
126
SfoI GGC'GCC
Cuts at: 2177 2177
Size: 7258
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7258
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7258
SmaI CCC'GGG
Cuts at: 1872 4585 1872
Size: 2713 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4373 5362 6263 6525 820
Size: 197 926 2430 989 901 262 1553
Fragments arranged by size:
2430 1553 989 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5049 493
Size: 4556 2702
SpeI A'CTAG_T
Cuts at: 152 2936 152
Size: 2784 4474
SphI G_CATG'C
Cuts at: 1132 2999 4616 1132
Size: 1867 1617 3774
Fragments arranged by size:
3774 1867 1617
SspI AAT'ATT
Cuts at: 5 52 3141 3491 5182 5
Size: 47 3089 350 1691 2081
Fragments arranged by size:
3089 2081 1691 350 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7258
StyI C'CwwG_G
Cuts at: 513 1065 3896 4697 513
Size: 552 2831 801 3074
Fragments arranged by size:
3074 2831 801 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5285 5302 5455 5640 6085 325
Size: 1255 3705 17 153 185 445 1498
Fragments arranged by size:
3705 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3300 4299 5504
Size: 274 80 33 51 1443 1323 999 1205
Cuts at: 5504 7216 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1323 1205 999 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7218 7250 7218
Size: 32 7226
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7258
XbaI T'CTAG_A
Cuts at: 3260 4594 3260
Size: 1334 5924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3249 3249
Size: 7258
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7258
XmaI C'CCGG_G
Cuts at: 1870 4583 1870
Size: 2713 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 5387 4169
Size: 1218 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3258 5066 276
Size: 53 83 186 936 1724 1808 2468
Fragments arranged by size:
2468 1808 1724 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI