Restriction map for
pFN21AB7298
(Circular) (Six-base) MAPSORT of: pFN21AB7298.seq Check: 8344 from: 1 to: 7756
pFN21AB7298 7756 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5566 278
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2133 5099 1937
Size: 196 2966 4594
Fragments arranged by size:
4594 2966 196
Acc65I G'GTAC_C
Cuts at: 5077 5077
Size: 7756
AclI AA'CG_TT
Cuts at: 1563 5883 6256 1563
Size: 4320 373 3063
Fragments arranged by size:
4320 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3032 4887 5819 7203 7471 1632
Size: 1400 1855 932 1384 268 1917
Fragments arranged by size:
1917 1855 1400 1384 932 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7559
AflIII A'CryG_T
Cuts at: 1203 2592 3336 5046 6655 7632 1203
Size: 1389 744 1710 1609 977 1327
Fragments arranged by size:
1710 1609 1389 1327 977 744
AgeI A'CCGG_T
Cuts at: 6587 6587
Size: 7756
AhdI GACnn_n'nnGTC
Cuts at: 6485 6485
Size: 7756
AleI CACnn'nnGTG
Cuts at: 1166 2861 3320 1166
Size: 1695 459 5602
Fragments arranged by size:
5602 1695 459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2108 1184
Size: 924 6832
AlwNI CAG_nnn'CTG
Cuts at: 2251 3055 3693 3973 4910 4961 5499 7071 2251
Size: 804 638 280 937 51 538 1572 2936
Fragments arranged by size:
2936 1572 937 804 638 538 280 51
ApaI G_GGCC'C
Cuts at: 3706 3706
Size: 7756
ApaLI G'TGCA_C
Cuts at: 3002 3506 5813 6969 3002
Size: 504 2307 1156 3789
Fragments arranged by size:
3789 2307 1156 504
ApoI r'AATT_y
Cuts at: 1493 2495 5065 5291 5443 7552 1493
Size: 1002 2570 226 152 2109 1697
Fragments arranged by size:
2570 2109 1697 1002 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6310 160
Size: 872 5278 1606
Fragments arranged by size:
5278 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
AvaI C'yCGr_G
Cuts at: 1870 1943 2618 2755 4047 4266 5081 1870
Size: 73 675 137 1292 219 815 4545
Fragments arranged by size:
4545 1292 815 675 219 137 73
BamHI G'GATC_C
Cuts at: 5086 5453 5086
Size: 367 7389
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 3156 3232 3392 4576
Size: 325 217 627 541 828 76 160 1184
Cuts at: 4576 5077 6532 618
Size: 501 1455 1842
Fragments arranged by size:
1842 1455 1184 828 627 541 501 325
217 160 76
BanII G_rGCy'C
Cuts at: 1391 2160 3706 4079 4274 5075 1391
Size: 769 1546 373 195 801 4072
Fragments arranged by size:
4072 1546 801 769 373 195
BbeI G_GCGC'C
Cuts at: 3160 3396 4580 3160
Size: 236 1184 6336
Fragments arranged by size:
6336 1184 236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 2518 3020 928
Size: 971 299 320 502 5664
Fragments arranged by size:
5664 971 502 320 299
BbvCI CC'TCA_GC
Cuts at: 3993 4094 4250 4428 4518 4623 4799 3993
Size: 101 156 178 90 105 176 6950
Fragments arranged by size:
6950 178 176 156 105 101 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2927 2961 3629 3663 5947
Size: 34 50 34 906 34 668 34 2284
Cuts at: 5947 5981 1903
Size: 34 3678
Fragments arranged by size:
3678 2284 906 668 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4846 5648 6858 4846
Size: 802 1210 5744
Fragments arranged by size:
5744 1210 802
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2553 2562 2571
Size: 107 122 71 631 605 881 9 9
Cuts at: 2571 3776 4034 4228 4290 4619 6367 136
Size: 1205 258 194 62 329 1748 1525
Fragments arranged by size:
1748 1525 1205 881 631 605 329 258
194 122 107 71 62 9 9
BglII A'GATC_T
Cuts at: 3363 4662 7751 3363
Size: 1299 3089 3368
Fragments arranged by size:
3368 3089 1299
BlpI GC'TnA_GC
Cuts at: 2317 3974 4694 5173 2317
Size: 1657 720 479 4900
Fragments arranged by size:
4900 1657 720 479
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2619 3006 3237 3510 3706 5817 6973
Size: 324 505 387 231 273 196 2111 1156
Cuts at: 6973 1790
Size: 2573
Fragments arranged by size:
2573 2111 1156 505 387 324 273 231
196
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2863 4457 5487
Size: 416 53 83 399 24 1446 1594 1030
Cuts at: 5487 6445 442
Size: 958 1753
Fragments arranged by size:
1753 1594 1446 1030 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7756
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 7756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 3077 3248 4832 4965 6416 1239
Size: 1046 792 171 1584 133 1451 2579
Fragments arranged by size:
2579 1584 1451 1046 792 171 133
Bpu10I CC'TnA_GC
Cuts at: 3876 3993 4094 4250 4428 4518 4623 4799 3876
Size: 117 101 156 178 90 105 176 6833
Fragments arranged by size:
6833 178 176 156 117 105 101 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3473 3512 3827 4945 5038 5881 6746 7044 3473
Size: 39 315 1118 93 843 865 298 4185
Fragments arranged by size:
4185 1118 865 843 315 298 93 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 2652 4921 6419 882
Size: 617 720 433 2269 1498 2219
Fragments arranged by size:
2269 2219 1498 720 617 433
BsaAI yAC'GTr
Cuts at: 493 1204 3561 5547 493
Size: 711 2357 1986 2702
Fragments arranged by size:
2702 2357 1986 711
BsaBI GATnn'nnATC
Cuts at: 2348 7750 2348
Size: 5402 2354
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3157 3393 3585 4577
Size: 53 83 186 936 1624 236 192 992
Cuts at: 4577 5563 5945 6580 275
Size: 986 382 635 1451
Fragments arranged by size:
1624 1451 992 986 936 635 382 236
192 186 83 53
BsaWI w'CCGG_w
Cuts at: 4465 6189 6587 6861 7008 7419 4465
Size: 1724 398 274 147 411 4802
Fragments arranged by size:
4802 1724 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2660 2690 3881
Size: 30 165 30 1047 30 180 30 1191
Cuts at: 3881 3911 4218 4248 4367 4397 4830 4860 1178
Size: 30 307 30 119 30 433 30 4074
Fragments arranged by size:
4074 1191 1047 433 307 180 165 119
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 2651 2883 4141 4144 4310 4315 4640
Size: 1462 3 232 1258 3 166 5 325
Cuts at: 4640 1186
Size: 4302
Fragments arranged by size:
4302 1462 1258 325 232 166 5 3
3
BseYI C'CCAG_C
Cuts at: 4125 4255 4598 4738 4756 4841 4879 6959 7455
Size: 130 343 140 18 85 38 2080 496
Cuts at: 7455 4125
Size: 4426
Fragments arranged by size:
4426 2080 496 343 140 130 85 38
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 3094 3361 3698 1163
Size: 573 501 857 267 337 5221
Fragments arranged by size:
5221 857 573 501 337 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2173 3524 5206 5967 6995 1540
Size: 275 186 172 1351 1682 761 1028 2301
Fragments arranged by size:
2301 1682 1351 1028 761 275 186 172
BsiHKAI G_wGCw'C
Cuts at: 2160 2283 3006 3090 3510 3816 4409 5817 5902
Size: 123 723 84 420 306 593 1408 85
Cuts at: 5902 6973 2160
Size: 1071 2943
Fragments arranged by size:
2943 1408 1071 723 593 420 306 123
85 84
BsmI GAATG_Cn'
Cuts at: 5267 5360 5267
Size: 93 7663
BsmBI CGTCTCn'nnnn_
Cuts at: 2680 4676 2680
Size: 1996 5760
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2160 2283 2619 3006 3090 3237
Size: 399 324 46 123 336 387 84 147
Cuts at: 3237 3510 3706 3816 4079 4274 4409 5075 5817
Size: 273 196 110 263 195 135 666 742
Cuts at: 5817 5902 6973 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 399 387 336 324
273 263 196 195 147 135 123 110
85 84 46
BspEI T'CCGG_A
Cuts at: 4465 7419 4465
Size: 2954 4802
BspHI T'CATG_A
Cuts at: 2013 5645 7375 2013
Size: 3632 1730 2394
Fragments arranged by size:
3632 2394 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2204 2973 4074 5111 844
Size: 1360 769 1101 1037 3489
Fragments arranged by size:
3489 1360 1101 1037 769
BsrBI CCG'CTC
Cuts at: 1394 1823 3061 3459 3526 4134 4569 5643 1394
Size: 429 1238 398 67 608 435 1074 3507
Fragments arranged by size:
3507 1238 1074 608 435 429 398 67
BsrDI GCAATG_nn'
Cuts at: 65 1248 3630 6251 6425 65
Size: 1183 2382 2621 174 1396
Fragments arranged by size:
2621 2382 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2543 4647 6400 6587 1700
Size: 110 198 535 2104 1753 187 2869
Fragments arranged by size:
2869 2104 1753 535 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7756
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7756
BssSI C'ACGA_G
Cuts at: 5816 6828 5816
Size: 1012 6744
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2251 3514 3538 3693 794
Size: 1457 1263 24 155 4857
Fragments arranged by size:
4857 1457 1263 155 24
BstBI TT'CG_AA
Cuts at: 6560 6560
Size: 7756
BstEII G'GTnAC_C
Cuts at: 1184 2728 1184
Size: 1544 6212
BstYI r'GATC_y
Cuts at: 1972 3363 3654 4662 5086 5453 5838 5855 7307
Size: 1391 291 1008 424 367 385 17 1452
Cuts at: 7307 7393 7405 7751 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1391 1008 424 385 367 346
291 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4001 4001
Size: 7756
BtgI C'CryG_G
Cuts at: 513 1065 2443 2637 2811 3518 3760 4896 513
Size: 552 1378 194 174 707 242 1136 3373
Fragments arranged by size:
3373 1378 1136 707 552 242 194 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 2791 3268 487
Size: 683 56 815 750 477 4975
Fragments arranged by size:
4975 815 750 683 477 56
BtsI GCAGTG_nn'
Cuts at: 3082 4873 5272 6066 6086 3082
Size: 1791 399 794 20 4752
Fragments arranged by size:
4752 1791 794 399 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2050 2085 529
Size: 35 1486 35 6200
Fragments arranged by size:
6200 1486 35 35
DraI TTT'AAA
Cuts at: 5060 5060
Size: 7756
DraIII CAC_nnn'GTG
Cuts at: 3409 3409
Size: 7756
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 809
Size: 507 5447 1802
Fragments arranged by size:
5447 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2545 2554
Size: 54 1425 50 275 18 267 448 9
Cuts at: 2554 2875 2919 3521 3834 4366 4611 5203 6092
Size: 321 44 602 313 532 245 592 889
Cuts at: 6092 7721 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 602 592 532 448 321
313 275 267 245 54 50 44 43
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3521 5203 1537
Size: 275 1709 1682 4090
Fragments arranged by size:
4090 1709 1682 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2828 5686 1353
Size: 1475 2858 3423
Fragments arranged by size:
3423 2858 1475
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6343 6717 6863 387
Size: 1064 138 4754 374 146 1280
Fragments arranged by size:
4754 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2158 2158
Size: 7756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 3032 3077 3248 4832 4887 4965
Size: 393 653 747 45 171 1584 55 78
Cuts at: 4965 5819 6416 7203 7471 1239
Size: 854 597 787 268 1524
Fragments arranged by size:
1584 1524 854 787 747 653 597 393
268 171 78 55 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3845 4070 4727 3845
Size: 225 657 6874
Fragments arranged by size:
6874 657 225
EcoO109I rG'GnC_Cy
Cuts at: 3484 3703 3853 3997 4051 4221 4522 4563 4742
Size: 219 150 144 54 170 301 41 179
Cuts at: 4742 4973 3484
Size: 231 6267
Fragments arranged by size:
6267 301 231 219 179 170 150 144
54 41
EcoRI G'AATT_C
Cuts at: 5065 5443 5065
Size: 378 7378
EcoRV GAT'ATC
Cuts at: 2401 2836 3871 2401
Size: 435 1035 6286
Fragments arranged by size:
6286 1035 435
FspI TGC'GCA
Cuts at: 6262 6262
Size: 7756
HaeII r_GCGC'y
Cuts at: 1678 3160 3279 3396 4580 6903 1678
Size: 1482 119 117 1184 2323 2531
Fragments arranged by size:
2531 2323 1482 1184 119 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3855 3887 3994 4026 4218
Size: 32 135 32 2283 32 107 32 192
Cuts at: 4218 4250 4654 4686 6397 6429 6471 6503 1373
Size: 32 404 32 1711 32 42 32 2626
Fragments arranged by size:
2626 2283 1711 404 192 135 107 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3571 5100 5346 669
Size: 988 281 1633 1529 246 3079
Fragments arranged by size:
3079 1633 1529 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7756
HpaI GTT'AAC
Cuts at: 5346 5346
Size: 7756
KasI G'GCGC_C
Cuts at: 3156 3392 4576 3156
Size: 236 1184 6336
Fragments arranged by size:
6336 1184 236
KpnI G_GTAC'C
Cuts at: 5081 5081
Size: 7756
MfeI C'AATT_G
Cuts at: 5355 5355
Size: 7756
MluI A'CGCG_T
Cuts at: 7632 7632
Size: 7756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 6870 7054 1305
Size: 861 4704 184 2007
Fragments arranged by size:
4704 2007 861 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 2921 3836 4368 4613
Size: 54 1425 343 267 822 915 532 245
Cuts at: 4613 10
Size: 3153
Fragments arranged by size:
3153 1425 915 822 532 343 267 245
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 2861 2987 3320 3503 3635 3786
Size: 648 1644 51 126 333 183 132 151
Cuts at: 3786 5714 6073 6232 518
Size: 1928 359 159 2042
Fragments arranged by size:
2042 1928 1644 648 359 333 183 159
151 132 126 51
MspA1I CmG'CkG
Cuts at: 1606 2202 2445 3184 3520 3576 3664 3973 4517
Size: 596 243 739 336 56 88 309 544
Cuts at: 4517 4798 5172 5849 6997 7242 1606
Size: 281 374 677 1148 245 2120
Fragments arranged by size:
2120 1148 739 677 596 544 374 336
309 281 245 243 88 56
NaeI GCC'GGC
Cuts at: 4649 4649
Size: 7756
NarI GG'CG_CC
Cuts at: 3157 3393 4577 3157
Size: 236 1184 6336
Fragments arranged by size:
6336 1184 236
NcoI C'CATG_G
Cuts at: 513 1065 4896 513
Size: 552 3831 3373
Fragments arranged by size:
3831 3373 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7756
NgoMIV G'CCGG_C
Cuts at: 4647 4647
Size: 7756
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7756
NotI GC'GGCC_GC
Cuts at: 3521 5203 3521
Size: 1682 6074
NruI TCG'CGA
Cuts at: 2785 2785
Size: 7756
NspI r_CATG'y
Cuts at: 1132 1229 3448 3541 5114 6659 1132
Size: 97 2219 93 1573 1545 2229
Fragments arranged by size:
2229 2219 1573 1545 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7756
PciI A'CATG_T
Cuts at: 6655 6655
Size: 7756
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2426 2917 3130 1108
Size: 1318 491 213 5734
Fragments arranged by size:
5734 1318 491 213
PfoI T'CCnGG_A
Cuts at: 2046 2334 4848 4917 2046
Size: 288 2514 69 4885
Fragments arranged by size:
4885 2514 288 69
PmeI GTTT'AAAC
Cuts at: 5060 5060
Size: 7756
PmlI CAC'GTG
Cuts at: 3561 3561
Size: 7756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5825 5857 7338 7370 5825
Size: 32 1481 32 6211
Fragments arranged by size:
6211 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3484 3853 3997 4051 4742 4973 3484
Size: 369 144 54 691 231 6267
Fragments arranged by size:
6267 691 369 231 144 54
PshAI GACnn'nnGTC
Cuts at: 1613 4473 1613
Size: 2860 4896
PsiI TTA'TAA
Cuts at: 5326 5326
Size: 7756
PspOMI G'GGCC_C
Cuts at: 3702 3702
Size: 7756
PstI C_TGCA'G
Cuts at: 830 2201 2247 3051 3663 3777 3969 4731 5108
Size: 1371 46 804 612 114 192 762 377
Cuts at: 5108 830
Size: 3478
Fragments arranged by size:
3478 1371 804 762 612 377 192 114
46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7756
PvuII CAG'CTG
Cuts at: 2202 3664 3973 4517 4798 2202
Size: 1462 309 544 281 5160
Fragments arranged by size:
5160 1462 544 309 281
SacI G_AGCT'C
Cuts at: 2160 2160
Size: 7756
SacII CC_GC'GG
Cuts at: 2446 3521 2446
Size: 1075 6681
SalI G'TCGA_C
Cuts at: 1936 5098 1936
Size: 3162 4594
SanDI GG'GwC_CC
Cuts at: 4742 4973 4742
Size: 231 7525
SapI GCTCTTCn'nnn_
Cuts at: 2828 2828
Size: 7756
SbfI CC_TGCA'GG
Cuts at: 3777 5108 3777
Size: 1331 6425
ScaI AGT'ACT
Cuts at: 6004 7716 6004
Size: 1712 6044
SexAI A'CCwGG_T
Cuts at: 2924 2924
Size: 7756
SfcI C'TryA_G
Cuts at: 826 1045 2197 2243 2668 3047 3659 3773 3965
Size: 219 1152 46 425 379 612 114 192
Cuts at: 3965 4584 4727 5104 6239 6920 7111 826
Size: 619 143 377 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 619 612 425 379
377 219 192 191 143 114 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 2553 2562 4228 2553
Size: 9 1666 6081
Fragments arranged by size:
6081 1666 9
SfoI GGC'GCC
Cuts at: 3158 3394 4578 3158
Size: 236 1184 6336
Fragments arranged by size:
6336 1184 236
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7756
SmaI CCC'GGG
Cuts at: 1872 4049 5083 1872
Size: 2177 1034 4545
Fragments arranged by size:
4545 2177 1034
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3488 3527 3806 4924 5017 5860
Size: 197 926 1545 39 279 1118 93 843
Cuts at: 5860 6761 7023 820
Size: 901 262 1553
Fragments arranged by size:
1553 1545 1118 926 901 843 279 262
197 93 39
SnaBI TAC'GTA
Cuts at: 493 5547 493
Size: 5054 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7756
SphI G_CATG'C
Cuts at: 1132 3541 5114 1132
Size: 2409 1573 3774
Fragments arranged by size:
3774 2409 1573
SspI AAT'ATT
Cuts at: 5 52 5680 5
Size: 47 5628 2081
Fragments arranged by size:
5628 2081 47
StuI AGG'CCT
Cuts at: 1517 4549 4994 1517
Size: 3032 445 4279
Fragments arranged by size:
4279 3032 445
StyI C'CwwG_G
Cuts at: 513 1065 2434 2470 2679 4559 4896 5195 513
Size: 552 1369 36 209 1880 337 299 3074
Fragments arranged by size:
3074 1880 1369 552 337 299 209 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2490 3094 4770 4855 5783 5800 5953
Size: 1255 910 604 1676 85 928 17 153
Cuts at: 5953 6138 6583 325
Size: 185 445 1498
Fragments arranged by size:
1676 1498 1255 928 910 604 445 185
153 85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 3294 3447
Size: 274 80 33 51 1443 645 672 153
Cuts at: 3447 6002 7714 96
Size: 2555 1712 138
Fragments arranged by size:
2555 1712 1443 672 645 274 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2450 2482 4367 4399 4830 4862 7716 7748 2450
Size: 32 1885 32 431 32 2854 32 2458
Fragments arranged by size:
2854 2458 1885 431 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7756
XbaI T'CTAG_A
Cuts at: 5092 5092
Size: 7756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4606 4606
Size: 7756
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7756
XmaI C'CCGG_G
Cuts at: 1870 4047 5081 1870
Size: 2177 1034 4545
Fragments arranged by size:
4545 2177 1034
XmnI GAAnn'nnTTC
Cuts at: 3365 5885 3365
Size: 2520 5236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5564 276
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I FalI FseI FspAI NsiI PacI PspXI
PsrI RsrII SrfI SwaI