Restriction map for
pFN21AB7297
(Circular) (Six-base) MAPSORT of: pFN21AB7297.seq Check: 6490 from: 1 to: 8383
pFN21AB7297 8383 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2995 4214 4978 2995
Size: 1219 764 6400
Fragments arranged by size:
6400 1219 764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6193 278
Size: 53 83 186 936 4657 2468
Fragments arranged by size:
4657 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5726 1937
Size: 3789 4594
Acc65I G'GTAC_C
Cuts at: 5704 5704
Size: 8383
AclI AA'CG_TT
Cuts at: 1563 6510 6883 1563
Size: 4947 373 3063
Fragments arranged by size:
4947 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4443 6446 7830 8098 1632
Size: 2811 2003 1384 268 1917
Fragments arranged by size:
2811 2003 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8186
AflIII A'CryG_T
Cuts at: 1203 2614 7282 8259 1203
Size: 1411 4668 977 1327
Fragments arranged by size:
4668 1411 1327 977
AgeI A'CCGG_T
Cuts at: 7214 7214
Size: 8383
AhdI GACnn_n'nnGTC
Cuts at: 7112 7112
Size: 8383
AleI CACnn'nnGTG
Cuts at: 1166 4674 1166
Size: 3508 4875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2288 2807 3135 3915 4550 5526 1184
Size: 1104 519 328 780 635 976 4041
Fragments arranged by size:
4041 1104 976 780 635 519 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 8351
AlwNI CAG_nnn'CTG
Cuts at: 2195 2514 2660 2962 4050 4819 5048 5330 5402
Size: 319 146 302 1088 769 229 282 72
Cuts at: 5402 5609 6126 7698 2195
Size: 207 517 1572 2880
Fragments arranged by size:
2880 1572 1088 769 517 319 302 282
229 207 146 72
ApaI G_GGCC'C
Cuts at: 3102 3579 3795 3859 4416 4585 5312 3102
Size: 477 216 64 557 169 727 6173
Fragments arranged by size:
6173 727 557 477 216 169 64
ApaLI G'TGCA_C
Cuts at: 3600 3890 6440 7596 3600
Size: 290 2550 1156 4387
Fragments arranged by size:
4387 2550 1156 290
ApoI r'AATT_y
Cuts at: 1493 5692 5918 6070 8179 1493
Size: 4199 226 152 2109 1697
Fragments arranged by size:
4199 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6937 160
Size: 872 5905 1606
Fragments arranged by size:
5905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
AvaI C'yCGr_G
Cuts at: 1870 1943 5018 5305 5564 5708 1870
Size: 73 3075 287 259 144 4545
Fragments arranged by size:
4545 3075 287 259 144 73
BamHI G'GATC_C
Cuts at: 2396 4611 5713 6080 2396
Size: 2215 1102 367 4699
Fragments arranged by size:
4699 2215 1102 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2310 3289 3916 4226 4763
Size: 325 217 627 523 979 627 310 537
Cuts at: 4763 5146 5651 5704 7159 618
Size: 383 505 53 1455 1842
Fragments arranged by size:
1842 1455 979 627 627 537 523 505
383 325 310 217 53
BanII G_rGCy'C
Cuts at: 1391 3102 3351 3516 3579 3683 3795 3859 4201
Size: 1711 249 165 63 104 112 64 342
Cuts at: 4201 4416 4585 5199 5312 5476 5702 1391
Size: 215 169 614 113 164 226 4072
Fragments arranged by size:
4072 1711 614 342 249 226 215 169
165 164 113 112 104 64 63
BbeI G_GCGC'C
Cuts at: 2314 3293 2314
Size: 979 7404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5212 5640 928
Size: 971 3313 428 3671
Fragments arranged by size:
3671 3313 971 428
BbvCI CC'TCA_GC
Cuts at: 3187 3187
Size: 8383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2993 3027 4896 4930 6574 6608 1903
Size: 34 1056 34 1869 34 1644 34 3678
Fragments arranged by size:
3678 1869 1644 1056 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4803 5473 6275 7485 4803
Size: 670 802 1210 5701
Fragments arranged by size:
5701 1210 802 670
BclI T'GATC_A
Cuts at: 1548 2937 1548
Size: 1389 6994
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2449 4439 5137
Size: 107 122 71 631 605 777 1990 698
Cuts at: 5137 6994 136
Size: 1857 1525
Fragments arranged by size:
1990 1857 1525 777 698 631 605 122
107 71
BglII A'GATC_T
Cuts at: 8378 8378
Size: 8383
BlpI GC'TnA_GC
Cuts at: 3054 3350 5800 3054
Size: 296 2450 5637
Fragments arranged by size:
5637 2450 296
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3022 3102 3163 3579 3604 3795 3833
Size: 343 889 80 61 416 25 191 38
Cuts at: 3833 3859 3894 3921 4416 4585 4914 5312 6444
Size: 26 35 27 495 169 329 398 1132
Cuts at: 6444 7600 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1132 889 495 416 398 343
329 191 169 80 61 38 35 27
26 25
BmgBI CAC'GTC
Cuts at: 1318 3985 1318
Size: 2667 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4918 4942 6114
Size: 416 53 83 399 24 3501 24 1172
Cuts at: 6114 7072 442
Size: 958 1753
Fragments arranged by size:
3501 1753 1172 958 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2227 2490 2594 3020 3365 3697 4274 4382
Size: 988 263 104 426 345 332 577 108
Cuts at: 4382 4562 5553 5563 5649 7043 1239
Size: 180 991 10 86 1394 2579
Fragments arranged by size:
2579 1394 991 988 577 426 345 332
263 180 108 104 86 10
Bpu10I CC'TnA_GC
Cuts at: 2963 3187 3787 2963
Size: 224 600 7559
Fragments arranged by size:
7559 600 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4187 4385 4556 6508 7373 7671 4187
Size: 198 171 1952 865 298 4899
Fragments arranged by size:
4899 1952 865 298 198 171
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2691 3203 3260 4641 7046 882
Size: 617 1192 512 57 1381 2405 2219
Fragments arranged by size:
2405 2219 1381 1192 617 512 57
BsaAI yAC'GTr
Cuts at: 493 1204 2170 3220 6174 493
Size: 711 966 1050 2954 2702
Fragments arranged by size:
2954 2702 1050 966 711
BsaBI GATnn'nnATC
Cuts at: 8377 8377
Size: 8383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2311 3290 3939 5129
Size: 53 83 186 936 778 979 649 1190
Cuts at: 5129 6190 6572 7207 275
Size: 1061 382 635 1451
Fragments arranged by size:
1451 1190 1061 979 936 778 649 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6816 7214 7488 7635 8046 6816
Size: 398 274 147 411 7153
Fragments arranged by size:
7153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 2993 3023 3350
Size: 30 165 30 1044 30 516 30 327
Cuts at: 3350 3380 3743 3773 4082 4112 5078 5108 1178
Size: 30 363 30 309 30 966 30 4453
Fragments arranged by size:
4453 1044 966 516 363 327 309 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3166 3175 3371 3839 4559 4620 4769 1186
Size: 1980 9 196 468 720 61 149 4800
Fragments arranged by size:
4800 1980 720 468 196 149 61 9
BseYI C'CCAG_C
Cuts at: 2182 2713 2734 3015 3027 3044 3411 3763 3858
Size: 531 21 281 12 17 367 352 95
Cuts at: 3858 3912 4171 4219 4578 4789 4918 4933 5499
Size: 54 259 48 359 211 129 15 566
Cuts at: 5499 7586 8082 2182
Size: 2087 496 2483
Fragments arranged by size:
2483 2087 566 531 496 367 359 352
281 259 211 129 95 54 48 21
17 15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2258 2339 2657 2864 3279 3320
Size: 573 339 183 81 318 207 415 41
Cuts at: 3320 3553 4232 4682 4817 5017 5185 1163
Size: 233 679 450 135 200 168 4361
Fragments arranged by size:
4361 679 573 450 415 339 318 233
207 200 183 168 135 81 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5245 5833 6594 7622 1540
Size: 275 186 3244 588 761 1028 2301
Fragments arranged by size:
3244 2301 1028 761 588 275 186
BsiHKAI G_wGCw'C
Cuts at: 3060 3225 3351 3604 3683 3894 4201 4539 4733
Size: 165 126 253 79 211 307 338 194
Cuts at: 4733 4955 5241 5476 6444 6529 7600 3060
Size: 222 286 235 968 85 1071 3843
Fragments arranged by size:
3843 1071 968 338 307 286 253 235
222 211 194 165 126 85 79
BsmI GAATG_Cn'
Cuts at: 2490 3497 3904 5894 5987 2490
Size: 1007 407 1990 93 4886
Fragments arranged by size:
4886 1990 1007 407 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 3022 3060 3102 3163 3225 3351
Size: 399 343 889 38 42 61 62 126
Cuts at: 3351 3516 3579 3604 3683 3795 3833 3859 3894
Size: 165 63 25 79 112 38 26 35
Cuts at: 3894 3921 4201 4416 4539 4585 4733 4914 4955
Size: 27 280 215 123 46 148 181 41
Cuts at: 4955 5199 5241 5312 5476 5702 6444 6529 7600
Size: 244 42 71 164 226 742 85 1071
Cuts at: 7600 1391
Size: 2174
Fragments arranged by size:
2174 1071 889 742 399 343 280 244
226 215 181 165 164 148 126 123
112 85 79 71 63 62 61 46
42 42 41 38 38 35 27 26
25
BspEI T'CCGG_A
Cuts at: 8046 8046
Size: 8383
BspHI T'CATG_A
Cuts at: 2865 3205 3993 6272 8002 2865
Size: 340 788 2279 1730 3246
Fragments arranged by size:
3246 2279 1730 788 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 2679 2718 2995 3497 4214 4515 4978
Size: 1199 636 39 277 502 717 301 463
Cuts at: 4978 5738 844
Size: 760 3489
Fragments arranged by size:
3489 1199 760 717 636 502 463 301
277 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2578 2749 6270 1394
Size: 429 755 171 3521 3507
Fragments arranged by size:
3521 3507 755 429 171
BsrDI GCAATG_nn'
Cuts at: 65 1248 4428 5023 6878 7052 65
Size: 1183 3180 595 1855 174 1396
Fragments arranged by size:
3180 1855 1396 1183 595 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3212 4295 4635 4707 4855 4878 5271
Size: 110 1402 1083 340 72 148 23 393
Cuts at: 5271 7027 7214 1700
Size: 1756 187 2869
Fragments arranged by size:
2869 1756 1402 1083 393 340 187 148
110 72 23
BsrGI T'GTAC_A
Cuts at: 96 2463 96
Size: 2367 6016
BssHII G'CGCG_C
Cuts at: 1925 2019 1925
Size: 94 8289
BssSI C'ACGA_G
Cuts at: 6443 7455 6443
Size: 1012 7371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2195 2501 3615 5146 5402 794
Size: 1401 306 1114 1531 256 3775
Fragments arranged by size:
3775 1531 1401 1114 306 256
BstBI TT'CG_AA
Cuts at: 7187 7187
Size: 8383
BstEII G'GTnAC_C
Cuts at: 1184 4291 1184
Size: 3107 5276
BstXI CCAn_nnnn'nTGG
Cuts at: 2713 3176 4360 4926 5447 2713
Size: 463 1184 566 521 5649
Fragments arranged by size:
5649 1184 566 521 463
BstYI r'GATC_y
Cuts at: 1972 2396 4215 4611 5713 6080 6465 6482 7934
Size: 424 1819 396 1102 367 385 17 1452
Cuts at: 7934 8020 8032 8378 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1819 1452 1102 424 396 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3103 3149 4884 5610 3103
Size: 46 1735 726 5876
Fragments arranged by size:
5876 1735 726 46
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 2827 4353 5097 513
Size: 552 939 63 760 1526 744 3799
Fragments arranged by size:
3799 1526 939 760 744 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7644
Fragments arranged by size:
7644 683 56
BtsI GCAGTG_nn'
Cuts at: 3461 3481 3628 3869 4147 5147 5342 5899 6693
Size: 20 147 241 278 1000 195 557 794
Cuts at: 6693 6713 3461
Size: 20 5131
Fragments arranged by size:
5131 1000 794 557 278 241 195 147
20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4582 4617 529
Size: 35 4018 35 4295
Fragments arranged by size:
4295 4018 35 35
DraI TTT'AAA
Cuts at: 5687 5687
Size: 8383
DraIII CAC_nnn'GTG
Cuts at: 2173 3073 4660 2173
Size: 900 1587 5896
Fragments arranged by size:
5896 1587 900
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2244 4899 5216 7390 809
Size: 507 928 2655 317 2174 1802
Fragments arranged by size:
2655 2174 1802 928 507 317
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3214 3661 4857
Size: 54 1425 50 275 18 1384 447 1196
Cuts at: 4857 5449 5830 6719 8348 8
Size: 592 381 889 1629 43
Fragments arranged by size:
1629 1425 1384 1196 889 592 447 381
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5830 1537
Size: 275 4018 4090
Fragments arranged by size:
4090 4018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4682 6313 1353
Size: 3329 1631 3423
Fragments arranged by size:
3423 3329 1631
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3986 4124 4694 6970 7344 7490
Size: 1064 138 2397 138 570 2276 374 146
Cuts at: 7490 387
Size: 1280
Fragments arranged by size:
2397 2276 1280 1064 570 374 146 138
138
EcoICRI GAG'CTC
Cuts at: 3349 3681 4199 5474 3349
Size: 332 518 1275 6258
Fragments arranged by size:
6258 1275 518 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2490 2594 3020 3365 3697 4274
Size: 393 595 263 104 426 345 332 577
Cuts at: 4274 4382 4443 4562 5553 5563 5649 6446 7043
Size: 108 61 119 991 10 86 797 597
Cuts at: 7043 7830 8098 1239
Size: 787 268 1524
Fragments arranged by size:
1524 991 797 787 597 595 577 426
393 345 332 268 263 119 108 104
86 61 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 3299 3002
Size: 297 8086
EcoO109I rG'GnC_Cy
Cuts at: 2286 2668 3040 3099 3504 3575 3576 3791 3837
Size: 382 372 59 405 71 1 215 46
Cuts at: 3837 4036 4413 4504 4582 5309 5381 5389 5496
Size: 199 377 91 78 727 72 8 107
Cuts at: 5496 2286
Size: 5173
Fragments arranged by size:
5173 727 405 382 377 372 215 199
107 91 78 72 71 59 46 8
1
EcoRI G'AATT_C
Cuts at: 5692 6070 5692
Size: 378 8005
EcoRV GAT'ATC
Cuts at: 5184 5184
Size: 8383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 8383
FspI TGC'GCA
Cuts at: 6889 6889
Size: 8383
HaeII r_GCGC'y
Cuts at: 1678 2314 3037 3293 4571 4714 7530 1678
Size: 636 723 256 1278 143 2816 2531
Fragments arranged by size:
2816 2531 1278 723 636 256 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2145 2177 3167 3199 4082
Size: 32 135 32 573 32 990 32 883
Cuts at: 4082 4114 4596 4628 4959 4991 5471 5488 5503
Size: 32 482 32 331 32 480 17 15
Cuts at: 5503 5520 7024 7056 7098 7130 1373
Size: 17 1504 32 42 32 2626
Fragments arranged by size:
2626 1504 990 883 573 482 480 331
135 42 32 32 32 32 32 32
32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 5727 5973 669
Size: 988 281 3789 246 3079
Fragments arranged by size:
3789 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8383
HpaI GTT'AAC
Cuts at: 5973 5973
Size: 8383
KasI G'GCGC_C
Cuts at: 2310 3289 2310
Size: 979 7404
KpnI G_GTAC'C
Cuts at: 5708 5708
Size: 8383
MfeI C'AATT_G
Cuts at: 5982 5982
Size: 8383
MluI A'CGCG_T
Cuts at: 8259 8259
Size: 8383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7497 7681 1305
Size: 6192 184 2007
Fragments arranged by size:
6192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3663 10
Size: 54 1425 343 1831 4730
Fragments arranged by size:
4730 1831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2483 2789 2864 3134 3174 3897
Size: 648 1044 273 306 75 270 40 723
Cuts at: 3897 4277 4352 4674 5445 6341 6700 6859 518
Size: 380 75 322 771 896 359 159 2042
Fragments arranged by size:
2042 1044 896 771 723 648 380 359
322 306 273 270 159 75 75 40
MspA1I CmG'CkG
Cuts at: 1606 2069 2498 2604 2734 3333 3526 3583 3912
Size: 463 429 106 130 599 193 57 329
Cuts at: 3912 4410 4984 5799 6476 7624 7869 1606
Size: 498 574 815 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 599 574 498 463
429 329 245 193 130 106 57
NaeI GCC'GGC
Cuts at: 4637 4709 4857 4637
Size: 72 148 8163
Fragments arranged by size:
8163 148 72
NarI GG'CG_CC
Cuts at: 2311 3290 2311
Size: 979 7404
NcoI C'CATG_G
Cuts at: 513 1065 2004 2827 4353 513
Size: 552 939 823 1526 4543
Fragments arranged by size:
4543 1526 939 823 552
NdeI CA'TA_TG
Cuts at: 387 3658 387
Size: 3271 5112
NgoMIV G'CCGG_C
Cuts at: 4635 4707 4855 4635
Size: 72 148 8163
Fragments arranged by size:
8163 148 72
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8383
NotI GC'GGCC_GC
Cuts at: 5830 5830
Size: 8383
NspI r_CATG'y
Cuts at: 1132 1229 2618 2765 2843 3139 5741 7286 1132
Size: 97 1389 147 78 296 2602 1545 2229
Fragments arranged by size:
2602 2229 1545 1389 296 147 97 78
PasI CC'CwG_GG
Cuts at: 1348 2902 3833 5492 1348
Size: 1554 931 1659 4239
Fragments arranged by size:
4239 1659 1554 931
PciI A'CATG_T
Cuts at: 2614 7282 2614
Size: 4668 3715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3770 4657 5330 5531 1108
Size: 2662 887 673 201 3960
Fragments arranged by size:
3960 2662 887 673 201
PfoI T'CCnGG_A
Cuts at: 3683 4539 5362 5413 5540 3683
Size: 856 823 51 127 6526
Fragments arranged by size:
6526 856 823 127 51
PmeI GTTT'AAAC
Cuts at: 5687 5687
Size: 8383
PmlI CAC'GTG
Cuts at: 2170 3220 2170
Size: 1050 7333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6452 6484 7965 7997 6452
Size: 32 1481 32 6838
Fragments arranged by size:
6838 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2286 2668 3040 3504 3837 4036 4504 5381 5389
Size: 382 372 464 333 199 468 877 8
Cuts at: 5389 5496 2286
Size: 107 5173
Fragments arranged by size:
5173 877 468 464 382 372 333 199
107 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8383
PsiI TTA'TAA
Cuts at: 5953 5953
Size: 8383
PspOMI G'GGCC_C
Cuts at: 3098 3575 3791 3855 4412 4581 5308 3098
Size: 477 216 64 557 169 727 6173
Fragments arranged by size:
6173 727 557 477 216 169 64
PstI C_TGCA'G
Cuts at: 830 2138 2200 3338 4028 4154 4592 4772 5063
Size: 1308 62 1138 690 126 438 180 291
Cuts at: 5063 5398 5407 5735 830
Size: 335 9 328 3478
Fragments arranged by size:
3478 1308 1138 690 438 335 328 291
180 126 62 9
PvuI CG_AT'CG
Cuts at: 2001 5245 2001
Size: 3244 5139
PvuII CAG'CTG
Cuts at: 2498 2734 3526 3912 2498
Size: 236 792 386 6969
Fragments arranged by size:
6969 792 386 236
SacI G_AGCT'C
Cuts at: 3351 3683 4201 5476 3351
Size: 332 518 1275 6258
Fragments arranged by size:
6258 1275 518 332
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 8383
SalI G'TCGA_C
Cuts at: 1936 5725 1936
Size: 3789 4594
SanDI GG'GwC_CC
Cuts at: 2286 4036 5496 2286
Size: 1750 1460 5173
Fragments arranged by size:
5173 1750 1460
SbfI CC_TGCA'GG
Cuts at: 5735 5735
Size: 8383
ScaI AGT'ACT
Cuts at: 6631 8343 6631
Size: 1712 6671
SexAI A'CCwGG_T
Cuts at: 3807 3999 4287 3807
Size: 192 288 7903
Fragments arranged by size:
7903 288 192
SfcI C'TryA_G
Cuts at: 826 1045 2134 2196 2779 3334 4024 4150 4588
Size: 219 1089 62 583 555 690 126 438
Cuts at: 4588 4768 5059 5394 5403 5731 6866 7547 7738
Size: 180 291 335 9 328 1135 681 191
Cuts at: 7738 826
Size: 1471
Fragments arranged by size:
1471 1135 1089 690 681 583 555 438
335 328 291 219 191 180 126 62
9
SfoI GGC'GCC
Cuts at: 2312 3291 2312
Size: 979 7404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
SgrAI Cr'CCGG_yG
Cuts at: 1700 4707 1700
Size: 3007 5376
SmaI CCC'GGG
Cuts at: 1872 5566 5710 1872
Size: 3694 144 4545
Fragments arranged by size:
4545 3694 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4202 4400 4571 6487 7388 7650
Size: 197 926 2259 198 171 1916 901 262
Cuts at: 7650 820
Size: 1553
Fragments arranged by size:
2259 1916 1553 926 901 262 198 197
171
SnaBI TAC'GTA
Cuts at: 493 6174 493
Size: 5681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8383
SphI G_CATG'C
Cuts at: 1132 2765 5741 1132
Size: 1633 2976 3774
Fragments arranged by size:
3774 2976 1633
SspI AAT'ATT
Cuts at: 5 52 2920 6307 5
Size: 47 2868 3387 2081
Fragments arranged by size:
3387 2868 2081 47
StuI AGG'CCT
Cuts at: 1517 4181 4598 5303 1517
Size: 2664 417 705 4597
Fragments arranged by size:
4597 2664 705 417
StyI C'CwwG_G
Cuts at: 513 1065 2004 2827 4317 4353 4389 5576 5822
Size: 552 939 823 1490 36 36 1187 246
Cuts at: 5822 513
Size: 3074
Fragments arranged by size:
3074 1490 1187 939 823 552 246 36
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 2292 3904 4735 5134 6410 6427
Size: 1255 659 53 1612 831 399 1276 17
Cuts at: 6427 6580 6765 7210 325
Size: 153 185 445 1498
Fragments arranged by size:
1612 1498 1276 1255 831 659 445 399
185 153 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2463 6629 8341
Size: 274 80 33 51 1443 486 4166 1712
Cuts at: 8341 96
Size: 138
Fragments arranged by size:
4166 1712 1443 486 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 2882 2914 2993 3025 3241 3273 3440
Size: 32 462 32 79 32 216 32 167
Cuts at: 3440 3472 3583 3615 4103 4135 5078 5110 8343
Size: 32 111 32 488 32 943 32 3233
Cuts at: 8343 8375 2388
Size: 32 2396
Fragments arranged by size:
3233 2396 943 488 462 216 167 111
79 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5048 1316
Size: 3732 4651
XbaI T'CTAG_A
Cuts at: 5719 5719
Size: 8383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2380 4435 5139 2174
Size: 206 2055 704 5418
Fragments arranged by size:
5418 2055 704 206
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8383
XmaI C'CCGG_G
Cuts at: 1870 5564 5708 1870
Size: 3694 144 4545
Fragments arranged by size:
4545 3694 144
XmnI GAAnn'nnTTC
Cuts at: 6512 6512
Size: 8383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6191 276
Size: 53 83 186 936 4657 2468
Fragments arranged by size:
4657 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SapI SfiI SrfI SwaI