(Circular) (Six-base) MAPSORT of: pFN21AB7297.seq Check: 6490 from: 1 to: 8383 pFN21AB7297 8383 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2995 4214 4978 2995 Size: 1219 764 6400 Fragments arranged by size: 6400 1219 764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6193 278 Size: 53 83 186 936 4657 2468 Fragments arranged by size: 4657 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5726 1937 Size: 3789 4594 Acc65I G'GTAC_C Cuts at: 5704 5704 Size: 8383 AclI AA'CG_TT Cuts at: 1563 6510 6883 1563 Size: 4947 373 3063 Fragments arranged by size: 4947 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4443 6446 7830 8098 1632 Size: 2811 2003 1384 268 1917 Fragments arranged by size: 2811 2003 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8186 AflIII A'CryG_T Cuts at: 1203 2614 7282 8259 1203 Size: 1411 4668 977 1327 Fragments arranged by size: 4668 1411 1327 977 AgeI A'CCGG_T Cuts at: 7214 7214 Size: 8383 AhdI GACnn_n'nnGTC Cuts at: 7112 7112 Size: 8383 AleI CACnn'nnGTG Cuts at: 1166 4674 1166 Size: 3508 4875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2288 2807 3135 3915 4550 5526 1184 Size: 1104 519 328 780 635 976 4041 Fragments arranged by size: 4041 1104 976 780 635 519 328 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3974 4006 3974 Size: 32 8351 AlwNI CAG_nnn'CTG Cuts at: 2195 2514 2660 2962 4050 4819 5048 5330 5402 Size: 319 146 302 1088 769 229 282 72 Cuts at: 5402 5609 6126 7698 2195 Size: 207 517 1572 2880 Fragments arranged by size: 2880 1572 1088 769 517 319 302 282 229 207 146 72 ApaI G_GGCC'C Cuts at: 3102 3579 3795 3859 4416 4585 5312 3102 Size: 477 216 64 557 169 727 6173 Fragments arranged by size: 6173 727 557 477 216 169 64 ApaLI G'TGCA_C Cuts at: 3600 3890 6440 7596 3600 Size: 290 2550 1156 4387 Fragments arranged by size: 4387 2550 1156 290 ApoI r'AATT_y Cuts at: 1493 5692 5918 6070 8179 1493 Size: 4199 226 152 2109 1697 Fragments arranged by size: 4199 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6937 160 Size: 872 5905 1606 Fragments arranged by size: 5905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8383 AvaI C'yCGr_G Cuts at: 1870 1943 5018 5305 5564 5708 1870 Size: 73 3075 287 259 144 4545 Fragments arranged by size: 4545 3075 287 259 144 73 BamHI G'GATC_C Cuts at: 2396 4611 5713 6080 2396 Size: 2215 1102 367 4699 Fragments arranged by size: 4699 2215 1102 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2310 3289 3916 4226 4763 Size: 325 217 627 523 979 627 310 537 Cuts at: 4763 5146 5651 5704 7159 618 Size: 383 505 53 1455 1842 Fragments arranged by size: 1842 1455 979 627 627 537 523 505 383 325 310 217 53 BanII G_rGCy'C Cuts at: 1391 3102 3351 3516 3579 3683 3795 3859 4201 Size: 1711 249 165 63 104 112 64 342 Cuts at: 4201 4416 4585 5199 5312 5476 5702 1391 Size: 215 169 614 113 164 226 4072 Fragments arranged by size: 4072 1711 614 342 249 226 215 169 165 164 113 112 104 64 63 BbeI G_GCGC'C Cuts at: 2314 3293 2314 Size: 979 7404 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5212 5640 928 Size: 971 3313 428 3671 Fragments arranged by size: 3671 3313 971 428 BbvCI CC'TCA_GC Cuts at: 3187 3187 Size: 8383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2993 3027 4896 4930 6574 6608 1903 Size: 34 1056 34 1869 34 1644 34 3678 Fragments arranged by size: 3678 1869 1644 1056 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4803 5473 6275 7485 4803 Size: 670 802 1210 5701 Fragments arranged by size: 5701 1210 802 670 BclI T'GATC_A Cuts at: 1548 2937 1548 Size: 1389 6994 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2449 4439 5137 Size: 107 122 71 631 605 777 1990 698 Cuts at: 5137 6994 136 Size: 1857 1525 Fragments arranged by size: 1990 1857 1525 777 698 631 605 122 107 71 BglII A'GATC_T Cuts at: 8378 8378 Size: 8383 BlpI GC'TnA_GC Cuts at: 3054 3350 5800 3054 Size: 296 2450 5637 Fragments arranged by size: 5637 2450 296 Bme1580I G_kGCm'C Cuts at: 1790 2133 3022 3102 3163 3579 3604 3795 3833 Size: 343 889 80 61 416 25 191 38 Cuts at: 3833 3859 3894 3921 4416 4585 4914 5312 6444 Size: 26 35 27 495 169 329 398 1132 Cuts at: 6444 7600 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1132 889 495 416 398 343 329 191 169 80 61 38 35 27 26 25 BmgBI CAC'GTC Cuts at: 1318 3985 1318 Size: 2667 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4918 4942 6114 Size: 416 53 83 399 24 3501 24 1172 Cuts at: 6114 7072 442 Size: 958 1753 Fragments arranged by size: 3501 1753 1172 958 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2227 2490 2594 3020 3365 3697 4274 4382 Size: 988 263 104 426 345 332 577 108 Cuts at: 4382 4562 5553 5563 5649 7043 1239 Size: 180 991 10 86 1394 2579 Fragments arranged by size: 2579 1394 991 988 577 426 345 332 263 180 108 104 86 10 Bpu10I CC'TnA_GC Cuts at: 2963 3187 3787 2963 Size: 224 600 7559 Fragments arranged by size: 7559 600 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4187 4385 4556 6508 7373 7671 4187 Size: 198 171 1952 865 298 4899 Fragments arranged by size: 4899 1952 865 298 198 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2691 3203 3260 4641 7046 882 Size: 617 1192 512 57 1381 2405 2219 Fragments arranged by size: 2405 2219 1381 1192 617 512 57 BsaAI yAC'GTr Cuts at: 493 1204 2170 3220 6174 493 Size: 711 966 1050 2954 2702 Fragments arranged by size: 2954 2702 1050 966 711 BsaBI GATnn'nnATC Cuts at: 8377 8377 Size: 8383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2311 3290 3939 5129 Size: 53 83 186 936 778 979 649 1190 Cuts at: 5129 6190 6572 7207 275 Size: 1061 382 635 1451 Fragments arranged by size: 1451 1190 1061 979 936 778 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6816 7214 7488 7635 8046 6816 Size: 398 274 147 411 7153 Fragments arranged by size: 7153 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2447 2477 2993 3023 3350 Size: 30 165 30 1044 30 516 30 327 Cuts at: 3350 3380 3743 3773 4082 4112 5078 5108 1178 Size: 30 363 30 309 30 966 30 4453 Fragments arranged by size: 4453 1044 966 516 363 327 309 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3166 3175 3371 3839 4559 4620 4769 1186 Size: 1980 9 196 468 720 61 149 4800 Fragments arranged by size: 4800 1980 720 468 196 149 61 9 BseYI C'CCAG_C Cuts at: 2182 2713 2734 3015 3027 3044 3411 3763 3858 Size: 531 21 281 12 17 367 352 95 Cuts at: 3858 3912 4171 4219 4578 4789 4918 4933 5499 Size: 54 259 48 359 211 129 15 566 Cuts at: 5499 7586 8082 2182 Size: 2087 496 2483 Fragments arranged by size: 2483 2087 566 531 496 367 359 352 281 259 211 129 95 54 48 21 17 15 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2258 2339 2657 2864 3279 3320 Size: 573 339 183 81 318 207 415 41 Cuts at: 3320 3553 4232 4682 4817 5017 5185 1163 Size: 233 679 450 135 200 168 4361 Fragments arranged by size: 4361 679 573 450 415 339 318 233 207 200 183 168 135 81 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5245 5833 6594 7622 1540 Size: 275 186 3244 588 761 1028 2301 Fragments arranged by size: 3244 2301 1028 761 588 275 186 BsiHKAI G_wGCw'C Cuts at: 3060 3225 3351 3604 3683 3894 4201 4539 4733 Size: 165 126 253 79 211 307 338 194 Cuts at: 4733 4955 5241 5476 6444 6529 7600 3060 Size: 222 286 235 968 85 1071 3843 Fragments arranged by size: 3843 1071 968 338 307 286 253 235 222 211 194 165 126 85 79 BsmI GAATG_Cn' Cuts at: 2490 3497 3904 5894 5987 2490 Size: 1007 407 1990 93 4886 Fragments arranged by size: 4886 1990 1007 407 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 3022 3060 3102 3163 3225 3351 Size: 399 343 889 38 42 61 62 126 Cuts at: 3351 3516 3579 3604 3683 3795 3833 3859 3894 Size: 165 63 25 79 112 38 26 35 Cuts at: 3894 3921 4201 4416 4539 4585 4733 4914 4955 Size: 27 280 215 123 46 148 181 41 Cuts at: 4955 5199 5241 5312 5476 5702 6444 6529 7600 Size: 244 42 71 164 226 742 85 1071 Cuts at: 7600 1391 Size: 2174 Fragments arranged by size: 2174 1071 889 742 399 343 280 244 226 215 181 165 164 148 126 123 112 85 79 71 63 62 61 46 42 42 41 38 38 35 27 26 25 BspEI T'CCGG_A Cuts at: 8046 8046 Size: 8383 BspHI T'CATG_A Cuts at: 2865 3205 3993 6272 8002 2865 Size: 340 788 2279 1730 3246 Fragments arranged by size: 3246 2279 1730 788 340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2043 2679 2718 2995 3497 4214 4515 4978 Size: 1199 636 39 277 502 717 301 463 Cuts at: 4978 5738 844 Size: 760 3489 Fragments arranged by size: 3489 1199 760 717 636 502 463 301 277 39 BsrBI CCG'CTC Cuts at: 1394 1823 2578 2749 6270 1394 Size: 429 755 171 3521 3507 Fragments arranged by size: 3521 3507 755 429 171 BsrDI GCAATG_nn' Cuts at: 65 1248 4428 5023 6878 7052 65 Size: 1183 3180 595 1855 174 1396 Fragments arranged by size: 3180 1855 1396 1183 595 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3212 4295 4635 4707 4855 4878 5271 Size: 110 1402 1083 340 72 148 23 393 Cuts at: 5271 7027 7214 1700 Size: 1756 187 2869 Fragments arranged by size: 2869 1756 1402 1083 393 340 187 148 110 72 23 BsrGI T'GTAC_A Cuts at: 96 2463 96 Size: 2367 6016 BssHII G'CGCG_C Cuts at: 1925 2019 1925 Size: 94 8289 BssSI C'ACGA_G Cuts at: 6443 7455 6443 Size: 1012 7371 BstAPI GCAn_nnn'nTGC Cuts at: 794 2195 2501 3615 5146 5402 794 Size: 1401 306 1114 1531 256 3775 Fragments arranged by size: 3775 1531 1401 1114 306 256 BstBI TT'CG_AA Cuts at: 7187 7187 Size: 8383 BstEII G'GTnAC_C Cuts at: 1184 4291 1184 Size: 3107 5276 BstXI CCAn_nnnn'nTGG Cuts at: 2713 3176 4360 4926 5447 2713 Size: 463 1184 566 521 5649 Fragments arranged by size: 5649 1184 566 521 463 BstYI r'GATC_y Cuts at: 1972 2396 4215 4611 5713 6080 6465 6482 7934 Size: 424 1819 396 1102 367 385 17 1452 Cuts at: 7934 8020 8032 8378 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1819 1452 1102 424 396 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3103 3149 4884 5610 3103 Size: 46 1735 726 5876 Fragments arranged by size: 5876 1735 726 46 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 2827 4353 5097 513 Size: 552 939 63 760 1526 744 3799 Fragments arranged by size: 3799 1526 939 760 744 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7644 Fragments arranged by size: 7644 683 56 BtsI GCAGTG_nn' Cuts at: 3461 3481 3628 3869 4147 5147 5342 5899 6693 Size: 20 147 241 278 1000 195 557 794 Cuts at: 6693 6713 3461 Size: 20 5131 Fragments arranged by size: 5131 1000 794 557 278 241 195 147 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4582 4617 529 Size: 35 4018 35 4295 Fragments arranged by size: 4295 4018 35 35 DraI TTT'AAA Cuts at: 5687 5687 Size: 8383 DraIII CAC_nnn'GTG Cuts at: 2173 3073 4660 2173 Size: 900 1587 5896 Fragments arranged by size: 5896 1587 900 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2244 4899 5216 7390 809 Size: 507 928 2655 317 2174 1802 Fragments arranged by size: 2655 2174 1802 928 507 317 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3214 3661 4857 Size: 54 1425 50 275 18 1384 447 1196 Cuts at: 4857 5449 5830 6719 8348 8 Size: 592 381 889 1629 43 Fragments arranged by size: 1629 1425 1384 1196 889 592 447 381 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5830 1537 Size: 275 4018 4090 Fragments arranged by size: 4090 4018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4682 6313 1353 Size: 3329 1631 3423 Fragments arranged by size: 3423 3329 1631 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3986 4124 4694 6970 7344 7490 Size: 1064 138 2397 138 570 2276 374 146 Cuts at: 7490 387 Size: 1280 Fragments arranged by size: 2397 2276 1280 1064 570 374 146 138 138 EcoICRI GAG'CTC Cuts at: 3349 3681 4199 5474 3349 Size: 332 518 1275 6258 Fragments arranged by size: 6258 1275 518 332 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2227 2490 2594 3020 3365 3697 4274 Size: 393 595 263 104 426 345 332 577 Cuts at: 4274 4382 4443 4562 5553 5563 5649 6446 7043 Size: 108 61 119 991 10 86 797 597 Cuts at: 7043 7830 8098 1239 Size: 787 268 1524 Fragments arranged by size: 1524 991 797 787 597 595 577 426 393 345 332 268 263 119 108 104 86 61 10 EcoNI CCTnn'n_nnAGG Cuts at: 3002 3299 3002 Size: 297 8086 EcoO109I rG'GnC_Cy Cuts at: 2286 2668 3040 3099 3504 3575 3576 3791 3837 Size: 382 372 59 405 71 1 215 46 Cuts at: 3837 4036 4413 4504 4582 5309 5381 5389 5496 Size: 199 377 91 78 727 72 8 107 Cuts at: 5496 2286 Size: 5173 Fragments arranged by size: 5173 727 405 382 377 372 215 199 107 91 78 72 71 59 46 8 1 EcoRI G'AATT_C Cuts at: 5692 6070 5692 Size: 378 8005 EcoRV GAT'ATC Cuts at: 5184 5184 Size: 8383 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3634 3634 Size: 8383 FspI TGC'GCA Cuts at: 6889 6889 Size: 8383 HaeII r_GCGC'y Cuts at: 1678 2314 3037 3293 4571 4714 7530 1678 Size: 636 723 256 1278 143 2816 2531 Fragments arranged by size: 2816 2531 1278 723 636 256 143 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2145 2177 3167 3199 4082 Size: 32 135 32 573 32 990 32 883 Cuts at: 4082 4114 4596 4628 4959 4991 5471 5488 5503 Size: 32 482 32 331 32 480 17 15 Cuts at: 5503 5520 7024 7056 7098 7130 1373 Size: 17 1504 32 42 32 2626 Fragments arranged by size: 2626 1504 990 883 573 482 480 331 135 42 32 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 5727 5973 669 Size: 988 281 3789 246 3079 Fragments arranged by size: 3789 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8383 HpaI GTT'AAC Cuts at: 5973 5973 Size: 8383 KasI G'GCGC_C Cuts at: 2310 3289 2310 Size: 979 7404 KpnI G_GTAC'C Cuts at: 5708 5708 Size: 8383 MfeI C'AATT_G Cuts at: 5982 5982 Size: 8383 MluI A'CGCG_T Cuts at: 8259 8259 Size: 8383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7497 7681 1305 Size: 6192 184 2007 Fragments arranged by size: 6192 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3663 10 Size: 54 1425 343 1831 4730 Fragments arranged by size: 4730 1831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2483 2789 2864 3134 3174 3897 Size: 648 1044 273 306 75 270 40 723 Cuts at: 3897 4277 4352 4674 5445 6341 6700 6859 518 Size: 380 75 322 771 896 359 159 2042 Fragments arranged by size: 2042 1044 896 771 723 648 380 359 322 306 273 270 159 75 75 40 MspA1I CmG'CkG Cuts at: 1606 2069 2498 2604 2734 3333 3526 3583 3912 Size: 463 429 106 130 599 193 57 329 Cuts at: 3912 4410 4984 5799 6476 7624 7869 1606 Size: 498 574 815 677 1148 245 2120 Fragments arranged by size: 2120 1148 815 677 599 574 498 463 429 329 245 193 130 106 57 NaeI GCC'GGC Cuts at: 4637 4709 4857 4637 Size: 72 148 8163 Fragments arranged by size: 8163 148 72 NarI GG'CG_CC Cuts at: 2311 3290 2311 Size: 979 7404 NcoI C'CATG_G Cuts at: 513 1065 2004 2827 4353 513 Size: 552 939 823 1526 4543 Fragments arranged by size: 4543 1526 939 823 552 NdeI CA'TA_TG Cuts at: 387 3658 387 Size: 3271 5112 NgoMIV G'CCGG_C Cuts at: 4635 4707 4855 4635 Size: 72 148 8163 Fragments arranged by size: 8163 148 72 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8383 NotI GC'GGCC_GC Cuts at: 5830 5830 Size: 8383 NspI r_CATG'y Cuts at: 1132 1229 2618 2765 2843 3139 5741 7286 1132 Size: 97 1389 147 78 296 2602 1545 2229 Fragments arranged by size: 2602 2229 1545 1389 296 147 97 78 PasI CC'CwG_GG Cuts at: 1348 2902 3833 5492 1348 Size: 1554 931 1659 4239 Fragments arranged by size: 4239 1659 1554 931 PciI A'CATG_T Cuts at: 2614 7282 2614 Size: 4668 3715 PflMI CCAn_nnn'nTGG Cuts at: 1108 3770 4657 5330 5531 1108 Size: 2662 887 673 201 3960 Fragments arranged by size: 3960 2662 887 673 201 PfoI T'CCnGG_A Cuts at: 3683 4539 5362 5413 5540 3683 Size: 856 823 51 127 6526 Fragments arranged by size: 6526 856 823 127 51 PmeI GTTT'AAAC Cuts at: 5687 5687 Size: 8383 PmlI CAC'GTG Cuts at: 2170 3220 2170 Size: 1050 7333 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6452 6484 7965 7997 6452 Size: 32 1481 32 6838 Fragments arranged by size: 6838 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2286 2668 3040 3504 3837 4036 4504 5381 5389 Size: 382 372 464 333 199 468 877 8 Cuts at: 5389 5496 2286 Size: 107 5173 Fragments arranged by size: 5173 877 468 464 382 372 333 199 107 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8383 PsiI TTA'TAA Cuts at: 5953 5953 Size: 8383 PspOMI G'GGCC_C Cuts at: 3098 3575 3791 3855 4412 4581 5308 3098 Size: 477 216 64 557 169 727 6173 Fragments arranged by size: 6173 727 557 477 216 169 64 PstI C_TGCA'G Cuts at: 830 2138 2200 3338 4028 4154 4592 4772 5063 Size: 1308 62 1138 690 126 438 180 291 Cuts at: 5063 5398 5407 5735 830 Size: 335 9 328 3478 Fragments arranged by size: 3478 1308 1138 690 438 335 328 291 180 126 62 9 PvuI CG_AT'CG Cuts at: 2001 5245 2001 Size: 3244 5139 PvuII CAG'CTG Cuts at: 2498 2734 3526 3912 2498 Size: 236 792 386 6969 Fragments arranged by size: 6969 792 386 236 SacI G_AGCT'C Cuts at: 3351 3683 4201 5476 3351 Size: 332 518 1275 6258 Fragments arranged by size: 6258 1275 518 332 SacII CC_GC'GG Cuts at: 2070 2070 Size: 8383 SalI G'TCGA_C Cuts at: 1936 5725 1936 Size: 3789 4594 SanDI GG'GwC_CC Cuts at: 2286 4036 5496 2286 Size: 1750 1460 5173 Fragments arranged by size: 5173 1750 1460 SbfI CC_TGCA'GG Cuts at: 5735 5735 Size: 8383 ScaI AGT'ACT Cuts at: 6631 8343 6631 Size: 1712 6671 SexAI A'CCwGG_T Cuts at: 3807 3999 4287 3807 Size: 192 288 7903 Fragments arranged by size: 7903 288 192 SfcI C'TryA_G Cuts at: 826 1045 2134 2196 2779 3334 4024 4150 4588 Size: 219 1089 62 583 555 690 126 438 Cuts at: 4588 4768 5059 5394 5403 5731 6866 7547 7738 Size: 180 291 335 9 328 1135 681 191 Cuts at: 7738 826 Size: 1471 Fragments arranged by size: 1471 1135 1089 690 681 583 555 438 335 328 291 219 191 180 126 62 9 SfoI GGC'GCC Cuts at: 2312 3291 2312 Size: 979 7404 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8383 SgrAI Cr'CCGG_yG Cuts at: 1700 4707 1700 Size: 3007 5376 SmaI CCC'GGG Cuts at: 1872 5566 5710 1872 Size: 3694 144 4545 Fragments arranged by size: 4545 3694 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 4202 4400 4571 6487 7388 7650 Size: 197 926 2259 198 171 1916 901 262 Cuts at: 7650 820 Size: 1553 Fragments arranged by size: 2259 1916 1553 926 901 262 198 197 171 SnaBI TAC'GTA Cuts at: 493 6174 493 Size: 5681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8383 SphI G_CATG'C Cuts at: 1132 2765 5741 1132 Size: 1633 2976 3774 Fragments arranged by size: 3774 2976 1633 SspI AAT'ATT Cuts at: 5 52 2920 6307 5 Size: 47 2868 3387 2081 Fragments arranged by size: 3387 2868 2081 47 StuI AGG'CCT Cuts at: 1517 4181 4598 5303 1517 Size: 2664 417 705 4597 Fragments arranged by size: 4597 2664 705 417 StyI C'CwwG_G Cuts at: 513 1065 2004 2827 4317 4353 4389 5576 5822 Size: 552 939 823 1490 36 36 1187 246 Cuts at: 5822 513 Size: 3074 Fragments arranged by size: 3074 1490 1187 939 823 552 246 36 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 2292 3904 4735 5134 6410 6427 Size: 1255 659 53 1612 831 399 1276 17 Cuts at: 6427 6580 6765 7210 325 Size: 153 185 445 1498 Fragments arranged by size: 1612 1498 1276 1255 831 659 445 399 185 153 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2463 6629 8341 Size: 274 80 33 51 1443 486 4166 1712 Cuts at: 8341 96 Size: 138 Fragments arranged by size: 4166 1712 1443 486 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2882 2914 2993 3025 3241 3273 3440 Size: 32 462 32 79 32 216 32 167 Cuts at: 3440 3472 3583 3615 4103 4135 5078 5110 8343 Size: 32 111 32 488 32 943 32 3233 Cuts at: 8343 8375 2388 Size: 32 2396 Fragments arranged by size: 3233 2396 943 488 462 216 167 111 79 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5048 1316 Size: 3732 4651 XbaI T'CTAG_A Cuts at: 5719 5719 Size: 8383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2174 2380 4435 5139 2174 Size: 206 2055 704 5418 Fragments arranged by size: 5418 2055 704 206 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8383 XmaI C'CCGG_G Cuts at: 1870 5564 5708 1870 Size: 3694 144 4545 Fragments arranged by size: 4545 3694 144 XmnI GAAnn'nnTTC Cuts at: 6512 6512 Size: 8383 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6191 276 Size: 53 83 186 936 4657 2468 Fragments arranged by size: 4657 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI