Restriction map for
pFN21AB7296
(Circular) (Six-base) MAPSORT of: pFN21AB7296.seq Check: 9056 from: 1 to: 8704
pFN21AB7296 8704 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6514 278
Size: 53 83 186 936 4978 2468
Fragments arranged by size:
4978 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6047 1937
Size: 4110 4594
Acc65I G'GTAC_C
Cuts at: 5147 6025 5147
Size: 878 7826
AclI AA'CG_TT
Cuts at: 1563 6831 7204 1563
Size: 5268 373 3063
Fragments arranged by size:
5268 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2190 3618 3771 6767 8151 8419 1632
Size: 491 67 1428 153 2996 1384 268 1917
Fragments arranged by size:
2996 1917 1428 1384 491 268 153 67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8507
AflIII A'CryG_T
Cuts at: 1203 2221 3356 3358 7603 8580 1203
Size: 1018 1135 2 4245 977 1327
Fragments arranged by size:
4245 1327 1135 1018 977 2
AgeI A'CCGG_T
Cuts at: 7535 7535
Size: 8704
AhdI GACnn_n'nnGTC
Cuts at: 7433 7433
Size: 8704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8704
AlwNI CAG_nnn'CTG
Cuts at: 2020 3743 3818 3962 3977 5237 5486 6447 8019
Size: 1723 75 144 15 1260 249 961 1572
Cuts at: 8019 2020
Size: 2705
Fragments arranged by size:
2705 1723 1572 1260 961 249 144 75
15
ApaLI G'TGCA_C
Cuts at: 6761 7917 6761
Size: 1156 7548
ApoI r'AATT_y
Cuts at: 1493 2331 2637 3332 3795 3951 4151 4534 4639
Size: 838 306 695 463 156 200 383 105
Cuts at: 4639 4701 4797 5015 5245 6013 6239 6391 8500
Size: 62 96 218 230 768 226 152 2109
Cuts at: 8500 1493
Size: 1697
Fragments arranged by size:
2109 1697 838 768 695 463 383 306
230 226 218 200 156 152 105 96
62
AseI AT'TA_AT
Cuts at: 160 1032 2156 2553 3507 4770 7258 160
Size: 872 1124 397 954 1263 2488 1606
Fragments arranged by size:
2488 1606 1263 1124 954 872 397
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8704
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2901 6029 1870
Size: 73 174 784 3128 4545
Fragments arranged by size:
4545 3128 784 174 73
AvrII C'CTAG_G
Cuts at: 2356 5555 2356
Size: 3199 5505
BamHI G'GATC_C
Cuts at: 5205 6034 6401 5205
Size: 829 367 7508
Fragments arranged by size:
7508 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 5147 6025 7480 618
Size: 325 217 627 287 3073 878 1455 1842
Fragments arranged by size:
3073 1842 1455 878 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 2691 5339 6023 1391
Size: 1300 2648 684 4072
Fragments arranged by size:
4072 2648 1300 684
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 3114 3205 4446 5479 928
Size: 971 963 252 91 1241 1033 4153
Fragments arranged by size:
4153 1241 1033 971 963 252 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6895 6929 1903
Size: 34 50 34 4874 34 3678
Fragments arranged by size:
4874 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6596 7806 6596
Size: 1210 7494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8704
BfrBI ATG'CAT
Cuts at: 4127 4127
Size: 8704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2059 7315
Size: 107 122 71 631 605 337 50 5256
Cuts at: 7315 136
Size: 1525
Fragments arranged by size:
5256 1525 631 605 337 122 107 71
50
BglII A'GATC_T
Cuts at: 4384 5396 8699 4384
Size: 1012 3303 4389
Fragments arranged by size:
4389 3303 1012
BlpI GC'TnA_GC
Cuts at: 2190 6121 2190
Size: 3931 4773
Bme1580I G_kGCm'C
Cuts at: 1790 4314 6765 7921 1790
Size: 2524 2451 1156 2573
Fragments arranged by size:
2573 2524 2451 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 2792 4099
Size: 416 53 83 399 24 898 477 1307
Cuts at: 4099 6435 7393 442
Size: 2336 958 1753
Fragments arranged by size:
2336 1753 1307 958 898 477 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3456 1056
Size: 2400 6304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3763 4293 3763
Size: 530 8174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 7364 1239
Size: 6125 2579
Bpu10I CC'TnA_GC
Cuts at: 3963 5067 3963
Size: 1104 7600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3900 4033 4640 5332 5668 6829 7694 7992 3900
Size: 133 607 692 336 1161 865 298 4612
Fragments arranged by size:
4612 1161 865 692 607 336 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7367 882
Size: 617 5868 2219
Fragments arranged by size:
5868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3359 3846 6495 493
Size: 711 2155 487 2649 2702
Fragments arranged by size:
2702 2649 2155 711 487
BsaBI GATnn'nnATC
Cuts at: 4128 5325 8698 4128
Size: 1197 3373 4134
Fragments arranged by size:
4134 3373 1197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6511 6893 7528 275
Size: 53 83 186 936 4978 382 635 1451
Fragments arranged by size:
4978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3642 5604 7137 7535 7809 7956 8367 3642
Size: 1962 1533 398 274 147 411 3979
Fragments arranged by size:
3979 1962 1533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5685 5715 5764 5782 5794
Size: 30 165 30 4282 30 49 18 12
Cuts at: 5794 5812 1178
Size: 18 4070
Fragments arranged by size:
4282 4070 165 49 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3710 5445 5772 5775 5790 1186
Size: 2524 1735 327 3 15 4100
Fragments arranged by size:
4100 2524 1735 327 15 3
BseYI C'CCAG_C
Cuts at: 5491 5542 5867 7907 8403 5491
Size: 51 325 2040 496 5792
Fragments arranged by size:
5792 2040 496 325 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4038 5223 1163
Size: 573 2302 1185 4644
Fragments arranged by size:
4644 2302 1185 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6154 6915 7943 1540
Size: 275 186 4153 761 1028 2301
Fragments arranged by size:
4153 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4101 5946 6765 6850 7921 4101
Size: 1845 819 85 1071 4884
Fragments arranged by size:
4884 1845 1071 819 85
BsmI GAATG_Cn'
Cuts at: 5995 6215 6308 5995
Size: 220 93 8391
Fragments arranged by size:
8391 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2691 4101 4314 5339 5946 6023 6765
Size: 399 901 1410 213 1025 607 77 742
Cuts at: 6765 6850 7921 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1410 1071 1025 901 742 607 399
213 85 77
BspEI T'CCGG_A
Cuts at: 8367 8367
Size: 8704
BspHI T'CATG_A
Cuts at: 2926 4717 6593 8323 2926
Size: 1791 1876 1730 3307
Fragments arranged by size:
3307 1876 1791 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4338 6059 844
Size: 3494 1721 3489
Fragments arranged by size:
3494 3489 1721
BsrBI CCG'CTC
Cuts at: 1394 1823 6591 1394
Size: 429 4768 3507
Fragments arranged by size:
4768 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 3463 4185 5359 5746 5862 7199
Size: 1183 1257 958 722 1174 387 116 1337
Cuts at: 7199 7373 65
Size: 174 1396
Fragments arranged by size:
1396 1337 1257 1183 1174 958 722 387
174 116
BsrFI r'CCGG_y
Cuts at: 1700 1810 7348 7535 1700
Size: 110 5538 187 2869
Fragments arranged by size:
5538 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4399 96
Size: 4303 4401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8704
BssSI C'ACGA_G
Cuts at: 5652 6764 7776 5652
Size: 1112 1012 6580
Fragments arranged by size:
6580 1112 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 2504 794
Size: 1226 484 6994
Fragments arranged by size:
6994 1226 484
BstBI TT'CG_AA
Cuts at: 7508 7508
Size: 8704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8704
BstYI r'GATC_y
Cuts at: 1972 3862 3899 4384 4914 5205 5396 6034 6401
Size: 1890 37 485 530 291 191 638 367
Cuts at: 6401 6786 6803 8255 8341 8353 8699 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1890 1452 638 530 485 385 367
346 291 191 86 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2590 4839 2590
Size: 2249 6455
BtgI C'CryG_G
Cuts at: 513 1065 5386 5734 513
Size: 552 4321 348 3483
Fragments arranged by size:
4321 3483 552 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7965
Fragments arranged by size:
7965 683 56
BtsI GCAGTG_nn'
Cuts at: 5317 6220 7014 7034 5317
Size: 903 794 20 6987
Fragments arranged by size:
6987 903 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5964 5999 529
Size: 35 5400 35 3234
Fragments arranged by size:
5400 3234 35 35
DraI TTT'AAA
Cuts at: 2430 3123 3558 6008 2430
Size: 693 435 2450 5126
Fragments arranged by size:
5126 2450 693 435
DraIII CAC_nnn'GTG
Cuts at: 4503 4781 4503
Size: 278 8426
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7711 809
Size: 507 6395 1802
Fragments arranged by size:
6395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6151 7040 8669
Size: 54 1425 50 275 18 4321 889 1629
Cuts at: 8669 8
Size: 43
Fragments arranged by size:
4321 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6151 1537
Size: 275 4339 4090
Fragments arranged by size:
4339 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2142 2571 3594 3681 3747 3765 6634
Size: 684 105 429 1023 87 66 18 2869
Cuts at: 6634 1353
Size: 3423
Fragments arranged by size:
3423 2869 1023 684 429 105 87 66
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5262 7291 7665 7811 387
Size: 1064 138 3673 2029 374 146 1280
Fragments arranged by size:
3673 2029 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2190 3618 3771 6767 7364 8151
Size: 393 491 67 1428 153 2996 597 787
Cuts at: 8151 8419 1239
Size: 268 1524
Fragments arranged by size:
2996 1524 1428 787 597 491 393 268
153 67
EcoO109I rG'GnC_Cy
Cuts at: 2353 4843 2353
Size: 2490 6214
EcoRI G'AATT_C
Cuts at: 3795 4639 6013 6391 3795
Size: 844 1374 378 6108
Fragments arranged by size:
6108 1374 844 378
EcoRV GAT'ATC
Cuts at: 3630 4963 3630
Size: 1333 7371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2325 3368 5418 5870 2325
Size: 1043 2050 452 5159
Fragments arranged by size:
5159 2050 1043 452
FspI TGC'GCA
Cuts at: 7210 7210
Size: 8704
HaeII r_GCGC'y
Cuts at: 1678 7851 1678
Size: 6173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 4717 4749 5594
Size: 32 135 32 689 32 2424 32 845
Cuts at: 5594 5626 5666 5698 7345 7377 7419 7451 1373
Size: 32 40 32 1647 32 42 32 2626
Fragments arranged by size:
2626 2424 1647 845 689 135 42 40
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5265 6048 6294 669
Size: 988 281 3327 783 246 3079
Fragments arranged by size:
3327 3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8704
HpaI GTT'AAC
Cuts at: 5265 6294 5265
Size: 1029 7675
KpnI G_GTAC'C
Cuts at: 5151 6029 5151
Size: 878 7826
MfeI C'AATT_G
Cuts at: 2247 3610 6303 2247
Size: 1363 2693 4648
Fragments arranged by size:
4648 2693 1363
MluI A'CGCG_T
Cuts at: 8580 8580
Size: 8704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4399 5728 5964 7818 8002 1305
Size: 3094 1329 236 1854 184 2007
Fragments arranged by size:
3094 2007 1854 1329 236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6882
Fragments arranged by size:
6882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 4107 5739 5967 6662 7021 7180
Size: 648 1372 1569 1632 228 695 359 159
Cuts at: 7180 518
Size: 2042
Fragments arranged by size:
2042 1632 1569 1372 695 648 359 228
159
MspA1I CmG'CkG
Cuts at: 1606 2020 3596 6120 6797 7945 8190 1606
Size: 414 1576 2524 677 1148 245 2120
Fragments arranged by size:
2524 2120 1576 1148 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 5386 5734 513
Size: 552 4321 348 3483
Fragments arranged by size:
4321 3483 552 348
NdeI CA'TA_TG
Cuts at: 387 2887 3852 387
Size: 2500 965 5239
Fragments arranged by size:
5239 2500 965
NheI G'CTAG_C
Cuts at: 1052 3452 1052
Size: 2400 6304
NotI GC'GGCC_GC
Cuts at: 6151 6151
Size: 8704
NsiI A_TGCA'T
Cuts at: 4129 4129
Size: 8704
NspI r_CATG'y
Cuts at: 1132 1229 3219 3542 3781 6062 7607 1132
Size: 97 1990 323 239 2281 1545 2229
Fragments arranged by size:
2281 2229 1990 1545 323 239 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8704
PciI A'CATG_T
Cuts at: 7603 7603
Size: 8704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8704
PmeI GTTT'AAAC
Cuts at: 6008 6008
Size: 8704
PmlI CAC'GTG
Cuts at: 3359 3359
Size: 8704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6773 6805 8286 8318 6773
Size: 32 1481 32 7159
Fragments arranged by size:
7159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 4843 2353
Size: 2490 6214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8704
PsiI TTA'TAA
Cuts at: 2364 2672 3338 3935 4871 6274 2364
Size: 308 666 597 936 1403 4794
Fragments arranged by size:
4794 1403 936 666 597 308
PstI C_TGCA'G
Cuts at: 830 2422 5175 6056 830
Size: 1592 2753 881 3478
Fragments arranged by size:
3478 2753 1592 881
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8704
PvuII CAG'CTG
Cuts at: 2020 3596 2020
Size: 1576 7128
SalI G'TCGA_C
Cuts at: 1936 6046 1936
Size: 4110 4594
SapI GCTCTTCn'nnn_
Cuts at: 2142 2571 2142
Size: 429 8275
SbfI CC_TGCA'GG
Cuts at: 6056 6056
Size: 8704
ScaI AGT'ACT
Cuts at: 6952 8664 6952
Size: 1712 6992
SexAI A'CCwGG_T
Cuts at: 2376 2917 4246 5143 2376
Size: 541 1329 897 5937
Fragments arranged by size:
5937 1329 897 541
SfcI C'TryA_G
Cuts at: 826 1045 2418 3680 3990 5171 6052 7187 7868
Size: 219 1373 1262 310 1181 881 1135 681
Cuts at: 7868 8059 826
Size: 191 1471
Fragments arranged by size:
1471 1373 1262 1181 1135 881 681 310
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8704
SmaI CCC'GGG
Cuts at: 1872 6031 1872
Size: 4159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 2901 3915 4012 4619 5347
Size: 197 926 174 784 1014 97 607 728
Cuts at: 5347 5683 6808 7709 7971 820
Size: 336 1125 901 262 1553
Fragments arranged by size:
1553 1125 1014 926 901 784 728 607
336 262 197 174 97
SnaBI TAC'GTA
Cuts at: 493 3846 6495 493
Size: 3353 2649 2702
Fragments arranged by size:
3353 2702 2649
SpeI A'CTAG_T
Cuts at: 152 2836 152
Size: 2684 6020
SphI G_CATG'C
Cuts at: 1132 3542 6062 1132
Size: 2410 2520 3774
Fragments arranged by size:
3774 2520 2410
SspI AAT'ATT
Cuts at: 5 52 3289 3760 4258 4748 6628 5
Size: 47 3237 471 498 490 1880 2081
Fragments arranged by size:
3237 2081 1880 498 490 471 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8704
StyI C'CwwG_G
Cuts at: 513 1065 2356 3786 5183 5386 5555 5734 6143
Size: 552 1291 1430 1397 203 169 179 409
Cuts at: 6143 513
Size: 3074
Fragments arranged by size:
3074 1430 1397 1291 552 409 203 179
169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4359 5967 6731 6748 6901 7086 7531
Size: 1255 2779 1608 764 17 153 185 445
Cuts at: 7531 325
Size: 1498
Fragments arranged by size:
2779 1608 1498 1255 764 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3647 4399 5228
Size: 274 80 33 51 1443 1670 752 829
Cuts at: 5228 6950 8662 96
Size: 1722 1712 138
Fragments arranged by size:
1722 1712 1670 1443 829 752 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 5685 5717 8664 8696 2058
Size: 32 3595 32 2947 32 2066
Fragments arranged by size:
3595 2947 2066 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4248 1316
Size: 2932 5772
XbaI T'CTAG_A
Cuts at: 6040 6040
Size: 8704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2253 3031 2253
Size: 778 7926
XhoI C'TCGA_G
Cuts at: 1943 2117 2901 1943
Size: 174 784 7746
Fragments arranged by size:
7746 784 174
XmaI C'CCGG_G
Cuts at: 1870 6029 1870
Size: 4159 4545
XmnI GAAnn'nnTTC
Cuts at: 2203 2223 2331 3006 6833 2203
Size: 20 108 675 3827 4074
Fragments arranged by size:
4074 3827 675 108 20
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6512 276
Size: 53 83 186 936 4978 2468
Fragments arranged by size:
4978 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI