Restriction map for
pFN21AB7293
(Circular) (Six-base) MAPSORT of: pFN21AB7293.seq Check: 8373 from: 1 to: 7795
pFN21AB7293 7795 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2310 4888 2310
Size: 2578 5217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5605 278
Size: 53 83 186 936 4069 2468
Fragments arranged by size:
4069 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5138 1937
Size: 3201 4594
Acc65I G'GTAC_C
Cuts at: 5116 5116
Size: 7795
AclI AA'CG_TT
Cuts at: 1563 5922 6295 1563
Size: 4359 373 3063
Fragments arranged by size:
4359 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2941 3622 5858 7242 7510 1632
Size: 1309 681 2236 1384 268 1917
Fragments arranged by size:
2236 1917 1384 1309 681 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7598
AflIII A'CryG_T
Cuts at: 1203 4347 6694 7671 1203
Size: 3144 2347 977 1327
Fragments arranged by size:
3144 2347 1327 977
AgeI A'CCGG_T
Cuts at: 6626 6626
Size: 7795
AhdI GACnn_n'nnGTC
Cuts at: 5042 6524 5042
Size: 1482 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2318 1184
Size: 1134 6661
AlwNI CAG_nnn'CTG
Cuts at: 2866 3940 4442 5538 7110 2866
Size: 1074 502 1096 1572 3551
Fragments arranged by size:
3551 1572 1096 1074 502
ApaI G_GGCC'C
Cuts at: 2642 3957 2642
Size: 1315 6480
ApaLI G'TGCA_C
Cuts at: 3292 5852 7008 3292
Size: 2560 1156 4079
Fragments arranged by size:
4079 2560 1156
ApoI r'AATT_y
Cuts at: 1493 5104 5330 5482 7591 1493
Size: 3611 226 152 2109 1697
Fragments arranged by size:
3611 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6349 160
Size: 872 5317 1606
Fragments arranged by size:
5317 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7795
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 4193 4740 4932 5120 1870
Size: 73 450 1800 547 192 188 4545
Fragments arranged by size:
4545 1800 547 450 192 188 73
BamHI G'GATC_C
Cuts at: 5125 5492 5125
Size: 367 7428
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 2372 2609 2755 2992
Size: 325 217 627 407 178 237 146 237
Cuts at: 2992 3017 3245 3992 4424 5116 6571 618
Size: 25 228 747 432 692 1455 1842
Fragments arranged by size:
1842 1455 747 692 627 432 407 325
237 237 228 217 178 146 25
BanII G_rGCy'C
Cuts at: 1391 2097 2241 2642 2657 3519 3579 3845 3957
Size: 706 144 401 15 862 60 266 112
Cuts at: 3957 4071 5114 1391
Size: 114 1043 4072
Fragments arranged by size:
4072 1043 862 706 401 266 144 114
112 60 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3433 3915 4275 4523 928
Size: 971 1534 482 360 248 4200
Fragments arranged by size:
4200 1534 971 482 360 248
BbvCI CC'TCA_GC
Cuts at: 3878 4010 3878
Size: 132 7663
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3007 3041 5986 6020 1903
Size: 34 1070 34 2945 34 3678
Fragments arranged by size:
3678 2945 1070 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5687 6897 5687
Size: 1210 6585
BclI T'GATC_A
Cuts at: 1548 3372 1548
Size: 1824 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3001 3318 6406
Size: 107 122 71 631 605 1329 317 3088
Cuts at: 6406 136
Size: 1525
Fragments arranged by size:
3088 1525 1329 631 605 317 122 107
71
BglII A'GATC_T
Cuts at: 4851 7790 4851
Size: 2939 4856
BlpI GC'TnA_GC
Cuts at: 2081 3573 4679 4746 5212 2081
Size: 1492 1106 67 466 4664
Fragments arranged by size:
4664 1492 1106 466 67
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2642 2758 3296 3957 3995 4092 4988
Size: 409 443 116 538 661 38 97 896
Cuts at: 4988 5856 7012 1790
Size: 868 1156 2573
Fragments arranged by size:
2573 1156 896 868 661 538 443 409
116 97 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3928 4703 5526
Size: 416 53 83 399 24 2511 775 823
Cuts at: 5526 6484 442
Size: 958 1753
Fragments arranged by size:
2511 1753 958 823 775 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2726 4055 5025 5033 6455 1239
Size: 1487 1329 970 8 1422 2579
Fragments arranged by size:
2579 1487 1422 1329 970 8
Bpu10I CC'TnA_GC
Cuts at: 3878 4010 4065 3878
Size: 132 55 7608
Fragments arranged by size:
7608 132 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 3080 5920 6785 7083 2267
Size: 813 2840 865 298 2979
Fragments arranged by size:
2979 2840 865 813 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2717 2806 2893 3673 4196 5009 6458
Size: 617 1218 89 87 780 523 813 1449
Cuts at: 6458 882
Size: 2219
Fragments arranged by size:
2219 1449 1218 813 780 617 523 89
87
BsaAI yAC'GTr
Cuts at: 493 1204 4033 5586 493
Size: 711 2829 1553 2702
Fragments arranged by size:
2829 2702 1553 711
BsaBI GATnn'nnATC
Cuts at: 2525 7789 2525
Size: 5264 2531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2040 4164 5602
Size: 53 83 186 936 497 10 2124 1438
Cuts at: 5602 5984 6619 275
Size: 382 635 1451
Fragments arranged by size:
2124 1451 1438 936 635 497 382 186
83 53 10
BsaWI w'CCGG_w
Cuts at: 6228 6626 6900 7047 7458 6228
Size: 398 274 147 411 6565
Fragments arranged by size:
6565 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2834 2864 2927
Size: 30 165 30 855 30 546 30 63
Cuts at: 2927 2957 5006 5036 1178
Size: 30 2049 30 3937
Fragments arranged by size:
3937 2049 855 546 165 63 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 3394 3743 3835 4490 4553 4606 4877
Size: 1225 983 349 92 655 63 53 271
Cuts at: 4877 1186
Size: 4104
Fragments arranged by size:
4104 1225 983 655 349 271 92 63
53
BseYI C'CCAG_C
Cuts at: 2129 2226 2330 2415 3240 3297 4390 4988 6998
Size: 97 104 85 825 57 1093 598 2010
Cuts at: 6998 7494 2129
Size: 496 2430
Fragments arranged by size:
2430 2010 1093 825 598 496 104 97
85 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 2255 1163
Size: 573 335 184 6703
Fragments arranged by size:
6703 573 335 184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2187 5245 6006 7034 1540
Size: 275 186 186 3058 761 1028 2301
Fragments arranged by size:
3058 2301 1028 761 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 2211 3296 3579 3795 3948 4038 4071 4752 5079
Size: 1085 283 216 153 90 33 681 327
Cuts at: 5079 5856 5941 7012 2211
Size: 777 85 1071 2994
Fragments arranged by size:
2994 1085 1071 777 681 327 283 216
153 90 85 33
BsmI GAATG_Cn'
Cuts at: 5306 5399 5306
Size: 93 7702
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 4929 2021
Size: 2908 4887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2199 2211 2241 2642 2657 2758
Size: 399 307 102 12 30 401 15 101
Cuts at: 2758 3296 3519 3579 3795 3845 3948 3957 3995
Size: 538 223 60 216 50 103 9 38
Cuts at: 3995 4038 4071 4092 4752 4988 5079 5114 5856
Size: 43 33 21 660 236 91 35 742
Cuts at: 5856 5941 7012 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 538 401 399 307
236 223 216 103 102 101 91 85
60 50 43 38 35 33 30 21
15 12 9
BspEI T'CCGG_A
Cuts at: 7458 7458
Size: 7795
BspHI T'CATG_A
Cuts at: 4323 5684 7414 4323
Size: 1361 1730 4704
Fragments arranged by size:
4704 1730 1361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 2310 2481 2745 3540 4178 4888 5150
Size: 1433 33 171 264 795 638 710 262
Cuts at: 5150 844
Size: 3489
Fragments arranged by size:
3489 1433 795 710 638 264 262 171
33
BsrBI CCG'CTC
Cuts at: 1394 1823 5682 1394
Size: 429 3859 3507
Fragments arranged by size:
3859 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 4140 6290 6464 65
Size: 1183 1542 1350 2150 174 1396
Fragments arranged by size:
2150 1542 1396 1350 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2325 6439 6626 1700
Size: 110 515 4114 187 2869
Fragments arranged by size:
4114 2869 515 187 110
BsrGI T'GTAC_A
Cuts at: 96 4998 96
Size: 4902 2893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7795
BssSI C'ACGA_G
Cuts at: 2336 5855 6867 2336
Size: 3519 1012 3264
Fragments arranged by size:
3519 3264 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 4060 794
Size: 1363 1903 4529
Fragments arranged by size:
4529 1903 1363
BstBI TT'CG_AA
Cuts at: 6599 6599
Size: 7795
BstEII G'GTnAC_C
Cuts at: 1184 2545 3089 1184
Size: 1361 544 5890
Fragments arranged by size:
5890 1361 544
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2750 2830 3349 3553 3787 4216 4465 2689
Size: 61 80 519 204 234 429 249 6019
Fragments arranged by size:
6019 519 429 249 234 204 80 61
BstYI r'GATC_y
Cuts at: 1972 4170 4611 4851 5125 5492 5877 5894 7346
Size: 2198 441 240 274 367 385 17 1452
Cuts at: 7346 7432 7444 7790 1972
Size: 86 12 346 1977
Fragments arranged by size:
2198 1977 1452 441 385 367 346 274
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2688 2796 3735 513
Size: 552 939 684 108 939 4573
Fragments arranged by size:
4573 939 939 684 552 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3532 3553 3760 4063 487
Size: 683 56 2306 21 207 303 4219
Fragments arranged by size:
4219 2306 683 303 207 56 21
BtsI GCAGTG_nn'
Cuts at: 2075 2092 5311 6105 6125 2075
Size: 17 3219 794 20 3745
Fragments arranged by size:
3745 3219 794 20 17
ClaI AT'CG_AT
Cuts at: 1554 2187 1554
Size: 633 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2587 2622 529
Size: 35 2023 35 5702
Fragments arranged by size:
5702 2023 35 35
DraI TTT'AAA
Cuts at: 5099 5099
Size: 7795
DraIII CAC_nnn'GTG
Cuts at: 2049 2710 3291 3762 4098 2049
Size: 661 581 471 336 5746
Fragments arranged by size:
5746 661 581 471 336
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2978 6802 809
Size: 507 1662 3824 1802
Fragments arranged by size:
3824 1802 1662 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2697 2820 3054
Size: 54 1425 50 275 18 867 123 234
Cuts at: 3054 3135 3165 4027 4257 4582 4630 5242 6131
Size: 81 30 862 230 325 48 612 889
Cuts at: 6131 7760 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 867 862 612 325 275
234 230 123 81 54 50 48 43
30 18
EagI C'GGCC_G
Cuts at: 1537 1812 5242 1537
Size: 275 3430 4090
Fragments arranged by size:
4090 3430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3800 5725 1353
Size: 2447 1925 3423
Fragments arranged by size:
3423 2447 1925
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2984 3956 6382 6756 6902 387
Size: 1064 138 1395 972 2426 374 146 1280
Fragments arranged by size:
2426 1395 1280 1064 972 374 146 138
EcoICRI GAG'CTC
Cuts at: 3577 4069 3577
Size: 492 7303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2726 2941 3622 4055 5025 5033 5858
Size: 393 1094 215 681 433 970 8 825
Cuts at: 5858 6455 7242 7510 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1094 970 825 787 681 597 433
393 268 215 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2741 3455 3899 4178 4469 4895 5024 2285
Size: 456 714 444 279 291 426 129 5056
Fragments arranged by size:
5056 714 456 444 426 291 279 129
EcoO109I rG'GnC_Cy
Cuts at: 2734 3100 3126 3214 3953 3954 4150 4462 5029
Size: 366 26 88 739 1 196 312 567
Cuts at: 5029 2734
Size: 5500
Fragments arranged by size:
5500 739 567 366 312 196 88 26
1
EcoRI G'AATT_C
Cuts at: 5104 5482 5104
Size: 378 7417
FspI TGC'GCA
Cuts at: 2165 6301 2165
Size: 4136 3659
FspAI rTGC'GCAy
Cuts at: 2165 2165
Size: 7795
HaeII r_GCGC'y
Cuts at: 1678 2062 3716 4419 4449 6942 1678
Size: 384 1654 703 30 2493 2531
Fragments arranged by size:
2531 2493 1654 703 384 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2258 2290 2834
Size: 32 135 32 509 32 145 32 544
Cuts at: 2834 2866 2927 2959 4657 4689 5006 5028 5038
Size: 32 61 32 1698 32 317 22 10
Cuts at: 5038 5060 6436 6468 6510 6542 1373
Size: 22 1376 32 42 32 2626
Fragments arranged by size:
2626 1698 1376 544 509 317 145 135
61 42 32 32 32 32 32 32
32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 4822 5139 5385 669
Size: 988 281 2884 317 246 3079
Fragments arranged by size:
3079 2884 988 317 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7795
HpaI GTT'AAC
Cuts at: 5385 5385
Size: 7795
KpnI G_GTAC'C
Cuts at: 5120 5120
Size: 7795
MfeI C'AATT_G
Cuts at: 5394 5394
Size: 7795
MluI A'CGCG_T
Cuts at: 7671 7671
Size: 7795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3627 4482 4714 6909 7093 1305
Size: 2322 855 232 2195 184 2007
Fragments arranged by size:
2322 2195 2007 855 232 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2699 2822 3137 3167 4029
Size: 54 1425 343 867 123 315 30 862
Cuts at: 4029 10
Size: 3776
Fragments arranged by size:
3776 1425 867 862 343 315 123 54
30
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 2795 3347 3551 3734 3785 4217
Size: 648 1138 491 552 204 183 51 432
Cuts at: 4217 4328 5753 6112 6271 518
Size: 111 1425 359 159 2042
Fragments arranged by size:
2042 1425 1138 648 552 491 432 359
204 183 159 111 51
MspA1I CmG'CkG
Cuts at: 1606 2055 2108 3309 3466 3642 4394 5211 5888
Size: 449 53 1201 157 176 752 817 677
Cuts at: 5888 7036 7281 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1201 1148 817 752 677 449 245
176 157 53
NaeI GCC'GGC
Cuts at: 2327 2327
Size: 7795
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6304
Fragments arranged by size:
6304 939 552
NdeI CA'TA_TG
Cuts at: 387 2203 387
Size: 1816 5979
NgoMIV G'CCGG_C
Cuts at: 2325 2325
Size: 7795
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7795
NotI GC'GGCC_GC
Cuts at: 5242 5242
Size: 7795
NspI r_CATG'y
Cuts at: 1132 1229 2164 2309 2446 5153 6698 1132
Size: 97 935 145 137 2707 1545 2229
Fragments arranged by size:
2707 2229 1545 935 145 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7795
PciI A'CATG_T
Cuts at: 6694 6694
Size: 7795
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2648 2989 3144 3940 1108
Size: 1540 341 155 796 4963
Fragments arranged by size:
4963 1540 796 341 155
PfoI T'CCnGG_A
Cuts at: 2627 2742 2627
Size: 115 7680
PmeI GTTT'AAAC
Cuts at: 5099 5099
Size: 7795
PmlI CAC'GTG
Cuts at: 4033 4033
Size: 7795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5864 5896 7377 7409 5864
Size: 32 1481 32 6250
Fragments arranged by size:
6250 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2734 3100 3126 3214 4150 5029 2734
Size: 366 26 88 936 879 5500
Fragments arranged by size:
5500 936 879 366 88 26
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7795
PsiI TTA'TAA
Cuts at: 5365 5365
Size: 7795
PspOMI G'GGCC_C
Cuts at: 2638 3953 2638
Size: 1315 6480
PstI C_TGCA'G
Cuts at: 830 3269 5147 830
Size: 2439 1878 3478
Fragments arranged by size:
3478 2439 1878
PvuI CG_AT'CG
Cuts at: 2001 2187 2001
Size: 186 7609
PvuII CAG'CTG
Cuts at: 2108 3309 3466 3642 2108
Size: 1201 157 176 6261
Fragments arranged by size:
6261 1201 176 157
SacI G_AGCT'C
Cuts at: 3579 4071 3579
Size: 492 7303
SalI G'TCGA_C
Cuts at: 1936 5137 1936
Size: 3201 4594
SanDI GG'GwC_CC
Cuts at: 3126 3126
Size: 7795
SapI GCTCTTCn'nnn_
Cuts at: 3800 3800
Size: 7795
SbfI CC_TGCA'GG
Cuts at: 5147 5147
Size: 7795
ScaI AGT'ACT
Cuts at: 6043 7755 6043
Size: 1712 6083
SexAI A'CCwGG_T
Cuts at: 3758 3758
Size: 7795
SfcI C'TryA_G
Cuts at: 826 1045 3108 3265 5143 6278 6959 7150 826
Size: 219 2063 157 1878 1135 681 191 1471
Fragments arranged by size:
2063 1878 1471 1135 681 219 191 157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7795
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7795
SmaI CCC'GGG
Cuts at: 1872 5122 1872
Size: 3250 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 3095 5899 6800 7062 820
Size: 197 926 339 813 2804 901 262 1553
Fragments arranged by size:
2804 1553 926 901 813 339 262 197
SnaBI TAC'GTA
Cuts at: 493 5586 493
Size: 5093 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7795
SphI G_CATG'C
Cuts at: 1132 2164 2309 2446 5153 1132
Size: 1032 145 137 2707 3774
Fragments arranged by size:
3774 2707 1032 145 137
SspI AAT'ATT
Cuts at: 5 52 5719 5
Size: 47 5667 2081
Fragments arranged by size:
5667 2081 47
StuI AGG'CCT
Cuts at: 1517 2562 2942 4124 4554 1517
Size: 1045 380 1182 430 4758
Fragments arranged by size:
4758 1182 1045 430 380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2853 3120 4458 4579 5234 513
Size: 552 939 849 267 1338 121 655 3074
Fragments arranged by size:
3074 1338 939 849 655 552 267 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2240 2527 2681 2773 2794 3311 3663
Size: 1255 660 287 154 92 21 517 352
Cuts at: 3663 5822 5839 5992 6177 6622 325
Size: 2159 17 153 185 445 1498
Fragments arranged by size:
2159 1498 1255 660 517 445 352 287
185 154 153 92 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4998 6041 7753
Size: 274 80 33 51 1443 3021 1043 1712
Cuts at: 7753 96
Size: 138
Fragments arranged by size:
3021 1712 1443 1043 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4332 4364 7755 7787 4332
Size: 32 3391 32 4340
Fragments arranged by size:
4340 3391 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5020 1316
Size: 3704 4091
XbaI T'CTAG_A
Cuts at: 5131 5131
Size: 7795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2905 3064 3160 3834 4216 2707
Size: 198 159 96 674 382 6286
Fragments arranged by size:
6286 674 382 198 159 96
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7795
XmaI C'CCGG_G
Cuts at: 1870 5120 1870
Size: 3250 4545
XmnI GAAnn'nnTTC
Cuts at: 5924 5924
Size: 7795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5603 276
Size: 53 83 186 936 4069 2468
Fragments arranged by size:
4069 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoRV FalI FseI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI