Restriction map for
pFN21AB7292
(Circular) (Six-base) MAPSORT of: pFN21AB7292.seq Check: 4541 from: 1 to: 8287
pFN21AB7292 8287 bp
With 182 enzymes: SgfI *
October 14, 2009 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3695 5235 3695
Size: 1540 6747
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6097 278
Size: 53 83 186 936 4561 2468
Fragments arranged by size:
4561 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4230 5630 1937
Size: 2293 1400 4594
Fragments arranged by size:
4594 2293 1400
Acc65I G'GTAC_C
Cuts at: 5608 5608
Size: 8287
AclI AA'CG_TT
Cuts at: 1563 2665 6414 6787 1563
Size: 1102 3749 373 3063
Fragments arranged by size:
3749 3063 1102 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3486 5110 6350 7734 8002 1632
Size: 1854 1624 1240 1384 268 1917
Fragments arranged by size:
1917 1854 1624 1384 1240 268
AfeI AGC'GCT
Cuts at: 2111 2111
Size: 8287
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8090
AflIII A'CryG_T
Cuts at: 1203 2256 5331 7186 8163 1203
Size: 1053 3075 1855 977 1327
Fragments arranged by size:
3075 1855 1327 1053 977
AgeI A'CCGG_T
Cuts at: 2418 7118 2418
Size: 4700 3587
AhdI GACnn_n'nnGTC
Cuts at: 2437 7016 2437
Size: 4579 3708
AleI CACnn'nnGTG
Cuts at: 1166 3866 1166
Size: 2700 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2906 3368 5501 1184
Size: 1722 462 2133 3970
Fragments arranged by size:
3970 2133 1722 462
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 5013 5045 2234
Size: 32 2747 32 5476
Fragments arranged by size:
5476 2747 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2524 5227 5236 6030 7602 2524
Size: 2703 9 794 1572 3209
Fragments arranged by size:
3209 2703 1572 794 9
ApaLI G'TGCA_C
Cuts at: 3488 4748 4937 6344 7500 3488
Size: 1260 189 1407 1156 4275
Fragments arranged by size:
4275 1407 1260 1156 189
ApoI r'AATT_y
Cuts at: 1493 5596 5822 5974 8083 1493
Size: 4103 226 152 2109 1697
Fragments arranged by size:
4103 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6841 160
Size: 872 5809 1606
Fragments arranged by size:
5809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8287
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 5177 5473 5612 1870
Size: 73 249 2985 296 139 4545
Fragments arranged by size:
4545 2985 296 249 139 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4201 4234 5113 5146 4201
Size: 33 879 33 7342
Fragments arranged by size:
7342 879 33 33
BamHI G'GATC_C
Cuts at: 5617 5984 5617
Size: 367 7920
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2916 4711 4734 5485 5608
Size: 325 217 627 1129 1795 23 751 123
Cuts at: 5608 7063 618
Size: 1455 1842
Fragments arranged by size:
1842 1795 1455 1129 751 627 325 217
123 23
BanII G_rGCy'C
Cuts at: 1391 2016 2152 3744 3894 4120 5606 1391
Size: 625 136 1592 150 226 1486 4072
Fragments arranged by size:
4072 1592 1486 625 226 150 136
BbeI G_GCGC'C
Cuts at: 2920 2920
Size: 8287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4763 5373 928
Size: 971 2864 610 3842
Fragments arranged by size:
3842 2864 971 610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2082 2116 3010 3044 6478 6512 1903
Size: 34 145 34 894 34 3434 34 3678
Fragments arranged by size:
3678 3434 894 145 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5545 6179 7389 5545
Size: 634 1210 6443
Fragments arranged by size:
6443 1210 634
BclI T'GATC_A
Cuts at: 1548 4125 4176 1548
Size: 2577 51 5659
Fragments arranged by size:
5659 2577 51
BfrBI ATG'CAT
Cuts at: 4579 4579
Size: 8287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3312 6898 136
Size: 107 122 71 631 605 1640 3586 1525
Fragments arranged by size:
3586 1640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3724 4080 8282 3724
Size: 356 4202 3729
Fragments arranged by size:
4202 3729 356
BlpI GC'TnA_GC
Cuts at: 5249 5704 5249
Size: 455 7832
Bme1580I G_kGCm'C
Cuts at: 1790 2193 3492 3938 4338 4413 4716 4752 4941
Size: 403 1299 446 400 75 303 36 189
Cuts at: 4941 5490 6348 7504 1790
Size: 549 858 1156 2573
Fragments arranged by size:
2573 1299 1156 858 549 446 403 400
303 189 75 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8287
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6018 6976 442
Size: 416 53 83 399 24 4601 958 1753
Fragments arranged by size:
4601 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4403 1056
Size: 3347 4940
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5290 5290
Size: 8287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2479 2513 3021 3414 3865 3965 5039 5090
Size: 1240 34 508 393 451 100 1074 51
Cuts at: 5090 5306 6947 1239
Size: 216 1641 2579
Fragments arranged by size:
2579 1641 1240 1074 508 451 393 216
100 51 34
Bpu10I CC'TnA_GC
Cuts at: 2903 4119 2903
Size: 1216 7071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3782 6412 7277 7575 3782
Size: 2630 865 298 4494
Fragments arranged by size:
4494 2630 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4994 5278 6950 882
Size: 617 3495 284 1672 2219
Fragments arranged by size:
3495 2219 1672 617 284
BsaAI yAC'GTr
Cuts at: 493 1204 3310 4753 5332 6078 493
Size: 711 2106 1443 579 746 2702
Fragments arranged by size:
2702 2106 1443 746 711 579
BsaBI GATnn'nnATC
Cuts at: 4511 8281 4511
Size: 3770 4517
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2917 2925 5441 6094
Size: 53 83 186 936 1384 8 2516 653
Cuts at: 6094 6476 7111 275
Size: 382 635 1451
Fragments arranged by size:
2516 1451 1384 936 653 635 382 186
83 53 8
BsaWI w'CCGG_w
Cuts at: 2418 6720 7118 7392 7539 7950 2418
Size: 4302 398 274 147 411 2755
Fragments arranged by size:
4302 2755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 3387 3392 3417
Size: 30 165 30 831 30 1123 5 25
Cuts at: 3417 3422 4695 4725 5013 5043 5318 5348 1178
Size: 5 1273 30 288 30 275 30 4117
Fragments arranged by size:
4117 1273 1123 831 288 275 165 30
30 30 30 30 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3413 3994 4280 4892 4895 4898 5012 5040
Size: 2227 581 286 612 3 3 114 28
Cuts at: 5040 5279 5309 5396 5399 5402 1186
Size: 239 30 87 3 3 4071
Fragments arranged by size:
4071 2227 612 581 286 239 114 87
30 28 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2032 2798 4893 5489 7490 7986 2032
Size: 766 2095 596 2001 496 2333
Fragments arranged by size:
2333 2095 2001 766 596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 3169 3595 4069 5503 1163
Size: 573 801 632 426 474 1434 3947
Fragments arranged by size:
3947 1434 801 632 573 474 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 5737 6498 7526 1540
Size: 275 186 180 3556 761 1028 2301
Fragments arranged by size:
3556 2301 1028 761 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 3492 3894 4752 4941 5255 5343 5517 6348 6433
Size: 402 858 189 314 88 174 831 85
Cuts at: 6433 7504 3492
Size: 1071 4275
Fragments arranged by size:
4275 1071 858 831 402 314 189 174
88 85
BsmI GAATG_Cn'
Cuts at: 4663 5798 5891 4663
Size: 1135 93 7059
Fragments arranged by size:
7059 1135 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2109 3417 4760 2109
Size: 1308 1343 5636
Fragments arranged by size:
5636 1343 1308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2152 2193 3492 3744 3894 3938
Size: 399 226 136 41 1299 252 150 44
Cuts at: 3938 4120 4338 4413 4716 4752 4941 5255 5343
Size: 182 218 75 303 36 189 314 88
Cuts at: 5343 5490 5517 5606 6348 6433 7504 1391
Size: 147 27 89 742 85 1071 2174
Fragments arranged by size:
2174 1299 1071 742 399 314 303 252
226 218 189 182 150 147 136 89
88 85 75 44 41 36 27
BspEI T'CCGG_A
Cuts at: 7950 7950
Size: 8287
BspHI T'CATG_A
Cuts at: 3447 4104 4296 6176 7906 3447
Size: 657 192 1880 1730 3828
Fragments arranged by size:
3828 1880 1730 657 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2532 3695 5235 5403 5642 844
Size: 1688 1163 1540 168 239 3489
Fragments arranged by size:
3489 1688 1540 1163 239 168
BsrBI CCG'CTC
Cuts at: 1394 1823 2721 4608 5347 6174 1394
Size: 429 898 1887 739 827 3507
Fragments arranged by size:
3507 1887 898 827 739 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2469 3199 3948 4128 6782 6956 65
Size: 1183 1221 730 749 180 2654 174 1396
Fragments arranged by size:
2654 1396 1221 1183 749 730 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2418 6931 7118 1700
Size: 110 608 4513 187 2869
Fragments arranged by size:
4513 2869 608 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 4377 4509 96
Size: 2499 1782 132 3874
Fragments arranged by size:
3874 2499 1782 132
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8287
BssSI C'ACGA_G
Cuts at: 3091 5342 6347 7359 3091
Size: 2251 1005 1012 4019
Fragments arranged by size:
4019 2251 1012 1005
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 3283 3651 4658 5227 5236 794
Size: 1730 759 368 1007 569 9 3845
Fragments arranged by size:
3845 1730 1007 759 569 368 9
BstBI TT'CG_AA
Cuts at: 7091 7091
Size: 8287
BstEII G'GTnAC_C
Cuts at: 1184 4168 1184
Size: 2984 5303
BstXI CCAn_nnnn'nTGG
Cuts at: 4255 4823 4255
Size: 568 7719
BstYI r'GATC_y
Cuts at: 1972 2316 3724 4080 5617 5984 6369 6386 7838
Size: 344 1408 356 1537 367 385 17 1452
Cuts at: 7838 7924 7936 8282 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1537 1452 1408 385 367 356 346
344 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2609 2609
Size: 8287
BtgI C'CryG_G
Cuts at: 513 1065 2004 2343 3261 4929 5283 513
Size: 552 939 339 918 1668 354 3517
Fragments arranged by size:
3517 1668 939 918 552 354 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3336 3778 4025 4602 487
Size: 683 56 2110 442 247 577 4172
Fragments arranged by size:
4172 2110 683 577 442 247 56
BtsI GCAGTG_nn'
Cuts at: 2317 3583 4464 4552 5803 6597 6617 2317
Size: 1266 881 88 1251 794 20 3987
Fragments arranged by size:
3987 1266 1251 881 794 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8252
DraI TTT'AAA
Cuts at: 3175 5591 3175
Size: 2416 5871
DraIII CAC_nnn'GTG
Cuts at: 5155 5155
Size: 8287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 5446 7294 809
Size: 507 819 3311 1848 1802
Fragments arranged by size:
3311 1848 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2178 2434
Size: 54 1425 50 275 18 288 60 256
Cuts at: 2434 3304 5016 5403 5734 6623 8252 8
Size: 870 1712 387 331 889 1629 43
Fragments arranged by size:
1712 1629 1425 889 870 387 331 288
275 256 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 5734 1537
Size: 275 366 3556 4090
Fragments arranged by size:
4090 3556 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2344 2540 2801 5015 5373 5581 6217
Size: 806 185 196 261 2214 358 208 636
Cuts at: 6217 1353
Size: 3423
Fragments arranged by size:
3423 2214 806 636 358 261 208 196
185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4959 6874 7248 7394 387
Size: 1064 138 3370 1915 374 146 1280
Fragments arranged by size:
3370 1915 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3892 3892
Size: 8287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2479 2513 3021 3414 3486 3865 3965
Size: 393 847 34 508 393 72 379 100
Cuts at: 3965 5039 5090 5110 5306 6350 6947 7734 8002
Size: 1074 51 20 196 1044 597 787 268
Cuts at: 8002 1239
Size: 1524
Fragments arranged by size:
1524 1074 1044 847 787 597 508 393
393 379 268 196 100 72 51 34
20
EcoNI CCTnn'n_nnAGG
Cuts at: 5033 5033
Size: 8287
EcoO109I rG'GnC_Cy
Cuts at: 5165 5392 5165
Size: 227 8060
EcoRI G'AATT_C
Cuts at: 5596 5974 5596
Size: 378 7909
EcoRV GAT'ATC
Cuts at: 3445 3445
Size: 8287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4250 4250
Size: 8287
FspI TGC'GCA
Cuts at: 2828 6793 2828
Size: 3965 4322
HaeII r_GCGC'y
Cuts at: 1678 2113 2910 2920 3988 7434 1678
Size: 435 797 10 1068 3446 2531
Fragments arranged by size:
3446 2531 1068 797 435 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 2423 2455 2850
Size: 32 135 32 552 32 267 32 395
Cuts at: 2850 2882 2964 2996 3032 3064 4278 4310 6928
Size: 32 82 32 36 32 1214 32 2618
Cuts at: 6928 6960 7002 7034 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2618 1214 552 395 267 135 82
42 36 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5631 5877 669
Size: 988 281 3693 246 3079
Fragments arranged by size:
3693 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3199 3693 748
Size: 2451 494 5342
Fragments arranged by size:
5342 2451 494
HpaI GTT'AAC
Cuts at: 5877 5877
Size: 8287
KasI G'GCGC_C
Cuts at: 2916 2916
Size: 8287
KpnI G_GTAC'C
Cuts at: 5612 5612
Size: 8287
MfeI C'AATT_G
Cuts at: 2671 5886 2671
Size: 3215 5072
MluI A'CGCG_T
Cuts at: 8163 8163
Size: 8287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 3475 4635 7401 7585 1305
Size: 936 1234 1160 2766 184 2007
Fragments arranged by size:
2766 2007 1234 1160 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 3306 10
Size: 54 1425 343 288 1186 4991
Fragments arranged by size:
4991 1425 1186 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2888 3749 3866 4934 6245 6604
Size: 648 1347 375 861 117 1068 1311 359
Cuts at: 6604 6763 518
Size: 159 2042
Fragments arranged by size:
2042 1347 1311 1068 861 648 375 359
159 117
MspA1I CmG'CkG
Cuts at: 1606 5242 5248 5285 5703 6380 7528 7773 1606
Size: 3636 6 37 418 677 1148 245 2120
Fragments arranged by size:
3636 2120 1148 677 418 245 37 6
NarI GG'CG_CC
Cuts at: 2917 2917
Size: 8287
NcoI C'CATG_G
Cuts at: 513 1065 2004 3261 4929 513
Size: 552 939 1257 1668 3871
Fragments arranged by size:
3871 1668 1257 939 552
NdeI CA'TA_TG
Cuts at: 387 4728 387
Size: 4341 3946
NheI G'CTAG_C
Cuts at: 1052 4399 1052
Size: 3347 4940
NotI GC'GGCC_GC
Cuts at: 5734 5734
Size: 8287
NsiI A_TGCA'T
Cuts at: 4581 4581
Size: 8287
NspI r_CATG'y
Cuts at: 1132 1229 3286 4495 4579 4583 5645 7190 1132
Size: 97 2057 1209 84 4 1062 1545 2229
Fragments arranged by size:
2229 2057 1545 1209 1062 97 84 4
PasI CC'CwG_GG
Cuts at: 1348 2305 1348
Size: 957 7330
PciI A'CATG_T
Cuts at: 7186 7186
Size: 8287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2260 5113 1108
Size: 1131 21 2853 4282
Fragments arranged by size:
4282 2853 1131 21
PfoI T'CCnGG_A
Cuts at: 2319 3145 3219 3430 5139 5322 2319
Size: 826 74 211 1709 183 5284
Fragments arranged by size:
5284 1709 826 211 183 74
PmeI GTTT'AAAC
Cuts at: 5591 5591
Size: 8287
PmlI CAC'GTG
Cuts at: 3310 4753 3310
Size: 1443 6844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2266 5013 5045 6356 6388 7869 7901 2234
Size: 32 2747 32 1311 32 1481 32 2620
Fragments arranged by size:
2747 2620 1481 1311 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5392 5392
Size: 8287
PshAI GACnn'nnGTC
Cuts at: 1613 2681 1613
Size: 1068 7219
PsiI TTA'TAA
Cuts at: 5857 5857
Size: 8287
PstI C_TGCA'G
Cuts at: 830 2692 3033 3279 3459 5232 5247 5400 5639
Size: 1862 341 246 180 1773 15 153 239
Cuts at: 5639 830
Size: 3478
Fragments arranged by size:
3478 1862 1773 341 246 239 180 153
15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8287
PvuII CAG'CTG
Cuts at: 5248 5248
Size: 8287
SacI G_AGCT'C
Cuts at: 3894 3894
Size: 8287
SacII CC_GC'GG
Cuts at: 5286 5286
Size: 8287
SalI G'TCGA_C
Cuts at: 1936 5629 1936
Size: 3693 4594
SapI GCTCTTCn'nnn_
Cuts at: 2540 2801 5581 2540
Size: 261 2780 5246
Fragments arranged by size:
5246 2780 261
SbfI CC_TGCA'GG
Cuts at: 5400 5639 5400
Size: 239 8048
ScaI AGT'ACT
Cuts at: 6535 8247 6535
Size: 1712 6575
SexAI A'CCwGG_T
Cuts at: 4696 4696
Size: 8287
SfcI C'TryA_G
Cuts at: 826 1045 2502 2688 2788 3029 3275 3455 5228
Size: 219 1457 186 100 241 246 180 1773
Cuts at: 5228 5243 5396 5635 6770 7451 7642 826
Size: 15 153 239 1135 681 191 1471
Fragments arranged by size:
1773 1471 1457 1135 681 246 241 239
219 191 186 180 153 100 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 3312 3312
Size: 8287
SfoI GGC'GCC
Cuts at: 2918 2918
Size: 8287
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8287
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8287
SmaI CCC'GGG
Cuts at: 1872 5179 5614 1872
Size: 3307 435 4545
Fragments arranged by size:
4545 3307 435
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3761 6391 7292 7554 820
Size: 197 926 1818 2630 901 262 1553
Fragments arranged by size:
2630 1818 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6078 493
Size: 5585 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8287
SphI G_CATG'C
Cuts at: 1132 4579 5645 1132
Size: 3447 1066 3774
Fragments arranged by size:
3774 3447 1066
SspI AAT'ATT
Cuts at: 5 52 6211 5
Size: 47 6159 2081
Fragments arranged by size:
6159 2081 47
StuI AGG'CCT
Cuts at: 1517 2760 1517
Size: 1243 7044
StyI C'CwwG_G
Cuts at: 513 1065 2004 2850 2920 3261 3922 4716 4816
Size: 552 939 846 70 341 661 794 100
Cuts at: 4816 4929 5034 5726 513
Size: 113 105 692 3074
Fragments arranged by size:
3074 939 846 794 692 661 552 341
113 105 100 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2474 2832 4247 6314 6331 6484 6669
Size: 1255 894 358 1415 2067 17 153 185
Cuts at: 6669 7114 325
Size: 445 1498
Fragments arranged by size:
2067 1498 1415 1255 894 445 358 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3459 3847
Size: 274 80 33 51 1443 618 864 388
Cuts at: 3847 4377 4509 6533 8245 96
Size: 530 132 2024 1712 138
Fragments arranged by size:
2024 1712 1443 864 618 530 388 274
138 132 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3082 3114 3387 3419 4695 4727 8247 8279 3082
Size: 32 273 32 1276 32 3520 32 3090
Fragments arranged by size:
3520 3090 1276 273 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2282 2657 1316
Size: 966 375 6946
Fragments arranged by size:
6946 966 375
XbaI T'CTAG_A
Cuts at: 5623 5623
Size: 8287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 2857 3259 2302
Size: 555 402 7330
Fragments arranged by size:
7330 555 402
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8287
XmaI C'CCGG_G
Cuts at: 1870 5177 5612 1870
Size: 3307 435 4545
Fragments arranged by size:
4545 3307 435
XmnI GAAnn'nnTTC
Cuts at: 6416 6416
Size: 8287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6095 276
Size: 53 83 186 936 4561 2468
Fragments arranged by size:
4561 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbvCI BsiWI BstZ17I FseI FspAI
NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII
SanDI SrfI SwaI