Restriction map for
pFN21AB7283
(Circular) (Six-base) MAPSORT of: pFN21AB7283.seq Check: 8337 from: 1 to: 7375
pFN21AB7283 7375 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 2422
Size: 7375
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3540 5185 278
Size: 53 83 186 936 2004 1645 2468
Fragments arranged by size:
2468 2004 1645 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4718 1937
Size: 2781 4594
Acc65I G'GTAC_C
Cuts at: 2958 3291 4696 2958
Size: 333 1405 5637
Fragments arranged by size:
5637 1405 333
AclI AA'CG_TT
Cuts at: 1563 5502 5875 1563
Size: 3939 373 3063
Fragments arranged by size:
3939 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2682 3482 5438 6822 7090 1632
Size: 1050 800 1956 1384 268 1917
Fragments arranged by size:
1956 1917 1384 1050 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7178
AflIII A'CryG_T
Cuts at: 1203 4006 6274 7251 1203
Size: 2803 2268 977 1327
Fragments arranged by size:
2803 2268 1327 977
AgeI A'CCGG_T
Cuts at: 6206 6206
Size: 7375
AhdI GACnn_n'nnGTC
Cuts at: 6104 6104
Size: 7375
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2592 3953 1184
Size: 1408 1361 4606
Fragments arranged by size:
4606 1408 1361
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 7343
AlwNI CAG_nnn'CTG
Cuts at: 2425 4604 4646 5118 6690 2425
Size: 2179 42 472 1572 3110
Fragments arranged by size:
3110 2179 1572 472 42
ApaI G_GGCC'C
Cuts at: 4517 4517
Size: 7375
ApaLI G'TGCA_C
Cuts at: 5432 6588 5432
Size: 1156 6219
ApoI r'AATT_y
Cuts at: 1493 2522 2612 3130 3701 4684 4910 5062 7171
Size: 1029 90 518 571 983 226 152 2109
Cuts at: 7171 1493
Size: 1697
Fragments arranged by size:
2109 1697 1029 983 571 518 226 152
90
AseI AT'TA_AT
Cuts at: 160 1032 3933 5929 160
Size: 872 2901 1996 1606
Fragments arranged by size:
2901 1996 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 2838 3666 4305 4700 1870
Size: 73 209 686 828 639 395 4545
Fragments arranged by size:
4545 828 686 639 395 209 73
BamHI G'GATC_C
Cuts at: 4705 5072 4705
Size: 367 7008
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2958 3291 4696 6151
Size: 325 217 627 756 415 333 1405 1455
Cuts at: 6151 618
Size: 1842
Fragments arranged by size:
1842 1455 1405 756 627 415 333 325
217
BanII G_rGCy'C
Cuts at: 1391 2077 2113 2508 3905 4517 4694 1391
Size: 686 36 395 1397 612 177 4072
Fragments arranged by size:
4072 1397 686 612 395 177 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6404
BbvCI CC'TCA_GC
Cuts at: 2844 2954 4070 4322 2844
Size: 110 1116 252 5897
Fragments arranged by size:
5897 1116 252 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5566 5600 1903
Size: 34 3629 34 3678
Fragments arranged by size:
3678 3629 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2660 5267 6477 2660
Size: 2607 1210 3558
Fragments arranged by size:
3558 2607 1210
BclI T'GATC_A
Cuts at: 1548 4236 1548
Size: 2688 4687
BfrBI ATG'CAT
Cuts at: 3158 3158
Size: 7375
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3927 5986 136
Size: 107 122 71 631 605 2255 2059 1525
Fragments arranged by size:
2255 2059 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4206 7370 4206
Size: 3164 4211
BlpI GC'TnA_GC
Cuts at: 2112 4792 2112
Size: 2680 4695
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4517 5436 6592 1790
Size: 1876 851 919 1156 2573
Fragments arranged by size:
2573 1876 1156 919 851
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7375
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5106 6064 442
Size: 416 53 83 399 24 3689 958 1753
Fragments arranged by size:
3689 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 7375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2359 2447 3455 3900 6035 1239
Size: 1120 88 1008 445 2135 2579
Fragments arranged by size:
2579 2135 1120 1008 445 88
Bpu10I CC'TnA_GC
Cuts at: 2507 2844 2954 4070 4322 2507
Size: 337 110 1116 252 5560
Fragments arranged by size:
5560 1116 337 252 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 3530 5500 6365 6663 2047
Size: 1483 1970 865 298 2759
Fragments arranged by size:
2759 1970 1483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2163 4163 4454 4657 6038 882
Size: 617 664 2000 291 203 1381 2219
Fragments arranged by size:
2219 2000 1381 664 617 291 203
BsaAI yAC'GTr
Cuts at: 493 1204 5166 493
Size: 711 3962 2702
Fragments arranged by size:
3962 2702 711
BsaBI GATnn'nnATC
Cuts at: 7369 7369
Size: 7375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3537 5182 5564 6199
Size: 53 83 186 936 2004 1645 382 635
Cuts at: 6199 275
Size: 1451
Fragments arranged by size:
2004 1645 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5808 6206 6480 6627 7038 5808
Size: 398 274 147 411 6145
Fragments arranged by size:
6145 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 2384 2414 4453
Size: 30 165 30 915 30 36 30 2039
Cuts at: 4453 4483 1178
Size: 30 4070
Fragments arranged by size:
4070 2039 915 165 36 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2335 2342 2420 4458 1186
Size: 1149 7 78 2038 4103
Fragments arranged by size:
4103 2038 1149 78 7
BseYI C'CCAG_C
Cuts at: 2047 2203 3111 3472 3678 3754 3807 3865 3972
Size: 156 908 361 206 76 53 58 107
Cuts at: 3972 4243 4317 4437 6578 7074 2047
Size: 271 74 120 2141 496 2348
Fragments arranged by size:
2348 2141 908 496 361 271 206 156
120 107 76 74 58 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2892 3632 1163
Size: 573 1156 740 4906
Fragments arranged by size:
4906 1156 740 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3032 4825 5586 6614 1540
Size: 275 186 1031 1793 761 1028 2301
Fragments arranged by size:
2301 1793 1031 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 2508 4065 4344 4660 5436 5521 6592 2113
Size: 395 1557 279 316 776 85 1071 2896
Fragments arranged by size:
2896 1557 1071 776 395 316 279 85
BsmI GAATG_Cn'
Cuts at: 4886 4979 4886
Size: 93 7282
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2113 2508 3666 3905 4065 4344
Size: 399 287 36 395 1158 239 160 279
Cuts at: 4344 4517 4660 4694 5436 5521 6592 1391
Size: 173 143 34 742 85 1071 2174
Fragments arranged by size:
2174 1158 1071 742 399 395 287 279
239 173 160 143 85 36 34
BspEI T'CCGG_A
Cuts at: 7038 7038
Size: 7375
BspHI T'CATG_A
Cuts at: 3705 3789 5264 6994 3705
Size: 84 1475 1730 4086
Fragments arranged by size:
4086 1730 1475 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2246 2422 4730 844
Size: 1402 176 2308 3489
Fragments arranged by size:
3489 2308 1402 176
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 2284 5262 1394
Size: 429 395 66 2978 3507
Fragments arranged by size:
3507 2978 429 395 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 2454 5870 6044 65
Size: 1183 1206 3416 174 1396
Fragments arranged by size:
3416 1396 1206 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2961 4081 6019 6206 1700
Size: 110 1151 1120 1938 187 2869
Fragments arranged by size:
2869 1938 1151 1120 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7375
BssHII G'CGCG_C
Cuts at: 1925 2131 1925
Size: 206 7169
BssSI C'ACGA_G
Cuts at: 5435 6447 5435
Size: 1012 6363
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7375
BstBI TT'CG_AA
Cuts at: 6179 6179
Size: 7375
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7375
BstXI CCAn_nnnn'nTGG
Cuts at: 3754 3754
Size: 7375
BstYI r'GATC_y
Cuts at: 1972 2057 2421 4206 4705 5072 5457 5474 6926
Size: 85 364 1785 499 367 385 17 1452
Cuts at: 6926 7012 7024 7370 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1785 1452 499 385 367 364 346
86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2484 2859 4494 4631 513
Size: 552 1419 375 1635 137 3257
Fragments arranged by size:
3257 1635 1419 552 375 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3409 487
Size: 683 56 2183 4453
Fragments arranged by size:
4453 2183 683 56
BtsI GCAGTG_nn'
Cuts at: 4547 4891 5685 5705 4547
Size: 344 794 20 6217
Fragments arranged by size:
6217 794 344 20
ClaI AT'CG_AT
Cuts at: 1554 3032 1554
Size: 1478 5897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7340
DraI TTT'AAA
Cuts at: 4679 4679
Size: 7375
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6382 809
Size: 507 5066 1802
Fragments arranged by size:
5066 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 3036 3252
Size: 54 1425 50 275 18 765 441 216
Cuts at: 3252 4266 4606 4822 5711 7340 8
Size: 1014 340 216 889 1629 43
Fragments arranged by size:
1629 1425 1014 889 765 441 340 275
216 216 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4822 1537
Size: 275 3010 4090
Fragments arranged by size:
4090 3010 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 2918 3497 3555 3616 4070 4352 4452
Size: 840 725 579 58 61 454 282 100
Cuts at: 4452 5305 1353
Size: 853 3423
Fragments arranged by size:
3423 853 840 725 579 454 282 100
61 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5962 6336 6482 387
Size: 1064 138 4373 374 146 1280
Fragments arranged by size:
4373 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2111 2506 2111
Size: 395 6980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2359 2447 2682 3455 3482 3900 5438
Size: 393 727 88 235 773 27 418 1538
Cuts at: 5438 6035 6822 7090 1239
Size: 597 787 268 1524
Fragments arranged by size:
1538 1524 787 773 727 597 418 393
268 235 88 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2402 2429 2030
Size: 372 27 6976
Fragments arranged by size:
6976 372 27
EcoO109I rG'GnC_Cy
Cuts at: 2023 2091 2235 2268 4513 4514 4521 2023
Size: 68 144 33 2245 1 7 4877
Fragments arranged by size:
4877 2245 144 68 33 7 1
EcoRI G'AATT_C
Cuts at: 3130 3701 4684 5062 3130
Size: 571 983 378 5443
Fragments arranged by size:
5443 983 571 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 7375
FspI TGC'GCA
Cuts at: 5881 5881
Size: 7375
HaeII r_GCGC'y
Cuts at: 1678 6522 1678
Size: 4844 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 2384 2416 2821
Size: 32 135 32 746 32 34 32 405
Cuts at: 2821 2853 4453 4485 6016 6048 6090 6122 1373
Size: 32 1600 32 1531 32 42 32 2626
Fragments arranged by size:
2626 1600 1531 746 405 135 42 34
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2743 4719 4965 669
Size: 988 281 805 1976 246 3079
Fragments arranged by size:
3079 1976 988 805 281 246
HindIII A'AGCT_T
Cuts at: 748 3548 748
Size: 2800 4575
HpaI GTT'AAC
Cuts at: 2743 4965 2743
Size: 2222 5153
KpnI G_GTAC'C
Cuts at: 2962 3295 4700 2962
Size: 333 1405 5637
Fragments arranged by size:
5637 1405 333
MfeI C'AATT_G
Cuts at: 4974 4974
Size: 7375
MluI A'CGCG_T
Cuts at: 4006 7251 4006
Size: 3245 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3599 4053 4468 6489 6673 1305
Size: 2294 454 415 2021 184 2007
Fragments arranged by size:
2294 2021 2007 454 415 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 3038 3254 4268 4608
Size: 54 1425 343 765 441 216 1014 340
Cuts at: 4608 10
Size: 2777
Fragments arranged by size:
2777 1425 1014 765 441 343 340 216
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3935 4097 5333 5692 5851 518
Size: 648 2769 162 1236 359 159 2042
Fragments arranged by size:
2769 2042 1236 648 359 162 159
MspA1I CmG'CkG
Cuts at: 1606 3111 3143 3351 3472 3689 4321 4791 5468
Size: 1505 32 208 121 217 632 470 677
Cuts at: 5468 6616 6861 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1505 1148 677 632 470 245 217
208 121 32
NcoI C'CATG_G
Cuts at: 513 1065 2484 2859 4494 513
Size: 552 1419 375 1635 3394
Fragments arranged by size:
3394 1635 1419 552 375
NdeI CA'TA_TG
Cuts at: 387 3835 387
Size: 3448 3927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7375
NotI GC'GGCC_GC
Cuts at: 4822 4822
Size: 7375
NsiI A_TGCA'T
Cuts at: 3160 3160
Size: 7375
NspI r_CATG'y
Cuts at: 1132 1229 2806 3730 4733 6278 1132
Size: 97 1577 924 1003 1545 2229
Fragments arranged by size:
2229 1577 1545 1003 924 97
PasI CC'CwG_GG
Cuts at: 1348 2655 4517 1348
Size: 1307 1862 4206
Fragments arranged by size:
4206 1862 1307
PciI A'CATG_T
Cuts at: 6274 6274
Size: 7375
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 2604 2949 3455 4495 4604 1108
Size: 1317 179 345 506 1040 109 3879
Fragments arranged by size:
3879 1317 1040 506 345 179 109
PfoI T'CCnGG_A
Cuts at: 2277 2424 2277
Size: 147 7228
PmeI GTTT'AAAC
Cuts at: 4679 4679
Size: 7375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5444 5476 6957 6989 5444
Size: 32 1481 32 5830
Fragments arranged by size:
5830 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2235 4521 2091
Size: 144 2286 4945
Fragments arranged by size:
4945 2286 144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7375
PsiI TTA'TAA
Cuts at: 4945 4945
Size: 7375
PspOMI G'GGCC_C
Cuts at: 4513 4513
Size: 7375
PstI C_TGCA'G
Cuts at: 830 3658 4727 830
Size: 2828 1069 3478
Fragments arranged by size:
3478 2828 1069
PvuI CG_AT'CG
Cuts at: 2001 3032 2001
Size: 1031 6344
PvuII CAG'CTG
Cuts at: 3111 3143 3351 3472 3689 4321 3111
Size: 32 208 121 217 632 6165
Fragments arranged by size:
6165 632 217 208 121 32
SacI G_AGCT'C
Cuts at: 2113 2508 2113
Size: 395 6980
SalI G'TCGA_C
Cuts at: 1936 4717 1936
Size: 2781 4594
SanDI GG'GwC_CC
Cuts at: 2091 2091
Size: 7375
SapI GCTCTTCn'nnn_
Cuts at: 2193 4070 2193
Size: 1877 5498
SbfI CC_TGCA'GG
Cuts at: 4727 4727
Size: 7375
ScaI AGT'ACT
Cuts at: 2903 5623 7335 2903
Size: 2720 1712 2943
Fragments arranged by size:
2943 2720 1712
SexAI A'CCwGG_T
Cuts at: 2013 2013
Size: 7375
SfcI C'TryA_G
Cuts at: 826 1045 3007 3654 4723 5858 6539 6730 826
Size: 219 1962 647 1069 1135 681 191 1471
Fragments arranged by size:
1962 1471 1135 1069 681 647 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7375
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7375
SmaI CCC'GGG
Cuts at: 1872 2154 3668 4702 1872
Size: 282 1514 1034 4545
Fragments arranged by size:
4545 1514 1034 282
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2062 3545 5479 6380 6642 820
Size: 197 926 119 1483 1934 901 262 1553
Fragments arranged by size:
1934 1553 1483 926 901 262 197 119
SnaBI TAC'GTA
Cuts at: 493 5166 493
Size: 4673 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7375
SphI G_CATG'C
Cuts at: 1132 2806 4733 1132
Size: 1674 1927 3774
Fragments arranged by size:
3774 1927 1674
SspI AAT'ATT
Cuts at: 5 52 2766 5299 5
Size: 47 2714 2533 2081
Fragments arranged by size:
2714 2533 2081 47
StuI AGG'CCT
Cuts at: 1517 3921 4233 1517
Size: 2404 312 4659
Fragments arranged by size:
4659 2404 312
StyI C'CwwG_G
Cuts at: 513 1065 2367 2484 2547 2859 3049 3883 4452
Size: 552 1302 117 63 312 190 834 569
Cuts at: 4452 4494 4814 513
Size: 42 320 3074
Fragments arranged by size:
3074 1302 834 569 552 320 312 190
117 63 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3462 4451 5402 5419 5572 5757 6202
Size: 1255 1882 989 951 17 153 185 445
Cuts at: 6202 325
Size: 1498
Fragments arranged by size:
1882 1498 1255 989 951 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 3960 5621
Size: 274 80 33 51 1443 924 1059 1661
Cuts at: 5621 7333 96
Size: 1712 138
Fragments arranged by size:
1712 1661 1443 1059 924 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3613 3645 7335 7367 3613
Size: 32 3690 32 3621
Fragments arranged by size:
3690 3621 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3718 1316
Size: 2402 4973
XbaI T'CTAG_A
Cuts at: 4711 4711
Size: 7375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3430 3452 3430
Size: 22 7353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7375
XmaI C'CCGG_G
Cuts at: 1870 2152 3666 4700 1870
Size: 282 1514 1034 4545
Fragments arranged by size:
4545 1514 1034 282
XmnI GAAnn'nnTTC
Cuts at: 5504 5504
Size: 7375
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3538 5183 276
Size: 53 83 186 936 2004 1645 2468
Fragments arranged by size:
2468 2004 1645 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII
SfiI SfoI SrfI SwaI