Restriction map for
pFN21AB7282
(Circular) (Six-base) MAPSORT of: pFN21AB7282.seq Check: 5031 from: 1 to: 6820
pFN21AB7282 6820 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 4163 1937
Size: 2082 144 4594
Fragments arranged by size:
4594 2082 144
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 2089 4947 5320 1563
Size: 526 2858 373 3063
Fragments arranged by size:
3063 2858 526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4883 6267 6535 1632
Size: 3251 1384 268 1917
Fragments arranged by size:
3251 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 3564 3960 4014 5719 6696 1203
Size: 2361 396 54 1705 977 1327
Fragments arranged by size:
2361 1705 1327 977 396 54
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 5549 5549
Size: 6820
AleI CACnn'nnGTG
Cuts at: 1166 2657 3224 3476 3650 1166
Size: 1491 567 252 174 4336
Fragments arranged by size:
4336 1491 567 252 174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6820
AlwNI CAG_nnn'CTG
Cuts at: 3419 4563 6135 3419
Size: 1144 1572 4104
Fragments arranged by size:
4104 1572 1144
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 2543 2868 4042 4129 4355 4507 6616 1493
Size: 1050 325 1174 87 226 152 2109 1697
Fragments arranged by size:
2109 1697 1174 1050 325 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 2360 2367 4145 1870
Size: 73 417 7 1778 4545
Fragments arranged by size:
4545 1778 417 73 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3111 3144 3111
Size: 33 6787
BamHI G'GATC_C
Cuts at: 4150 4517 4150
Size: 367 6453
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3946 4141 5596 618
Size: 325 217 627 2159 195 1455 1842
Fragments arranged by size:
2159 1842 1455 627 325 217 195
BanII G_rGCy'C
Cuts at: 1391 2648 3354 3842 4139 1391
Size: 1257 706 488 297 4072
Fragments arranged by size:
4072 1257 706 488 297
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2362 2964 3021 928
Size: 971 463 602 57 4727
Fragments arranged by size:
4727 971 602 463 57
BbvCI CC'TCA_GC
Cuts at: 3536 3952 3536
Size: 416 6404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5011 5045 1903
Size: 34 50 34 2990 34 3678
Fragments arranged by size:
3678 2990 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3112 3672 3704 3786 4712 5922 3112
Size: 560 32 82 926 1210 4010
Fragments arranged by size:
4010 1210 926 560 82 32
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6820
BfrBI ATG'CAT
Cuts at: 2008 2257 2008
Size: 249 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5431 136
Size: 107 122 71 631 605 3759 1525
Fragments arranged by size:
3759 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2560 6815 2560
Size: 4255 2565
BlpI GC'TnA_GC
Cuts at: 4237 4237
Size: 6820
Bme1580I G_kGCm'C
Cuts at: 1790 2248 3951 4881 6037 1790
Size: 458 1703 930 1156 2573
Fragments arranged by size:
2573 1703 1156 930 458
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2651 3917 4551
Size: 416 53 83 399 24 1234 1266 634
Cuts at: 4551 5509 442
Size: 958 1753
Fragments arranged by size:
1753 1266 1234 958 634 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3663 3918 3967 3663
Size: 255 49 6516
Fragments arranged by size:
6516 255 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2219 2983 3441 5480 1239
Size: 803 177 764 458 2039 2579
Fragments arranged by size:
2579 2039 803 764 458 177
Bpu10I CC'TnA_GC
Cuts at: 3536 3952 3536
Size: 416 6404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4945 5810 6108 4945
Size: 865 298 5657
Fragments arranged by size:
5657 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3576 5483 882
Size: 617 2077 1907 2219
Fragments arranged by size:
2219 2077 1907 617
BsaAI yAC'GTr
Cuts at: 493 1204 3304 3567 3963 4611 493
Size: 711 2100 263 396 648 2702
Fragments arranged by size:
2702 2100 711 648 396 263
BsaBI GATnn'nnATC
Cuts at: 2565 6814 2565
Size: 4249 2571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4627 5009 5644 275
Size: 53 83 186 936 3094 382 635 1451
Fragments arranged by size:
3094 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3033 5253 5651 5925 6072 6483 3033
Size: 2220 398 274 147 411 3370
Fragments arranged by size:
3370 2220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3071 3101 1178
Size: 30 165 30 1668 30 4897
Fragments arranged by size:
4897 1668 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2603 2638 3095 1186
Size: 1030 387 35 457 4911
Fragments arranged by size:
4911 1030 457 387 35
BseYI C'CCAG_C
Cuts at: 3186 3257 3599 3607 6023 6519 3186
Size: 71 342 8 2416 496 3487
Fragments arranged by size:
3487 2416 496 342 71 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2952 3311 3828 1163
Size: 573 1216 359 517 4155
Fragments arranged by size:
4155 1216 573 517 359
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4270 5031 6059 1540
Size: 275 186 2269 761 1028 2301
Fragments arranged by size:
2301 2269 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3033 3309 3354 3842 4059 4881 4966 6037 3033
Size: 276 45 488 217 822 85 1071 3816
Fragments arranged by size:
3816 1071 822 488 276 217 85 45
BsmI GAATG_Cn'
Cuts at: 3985 4331 4424 3985
Size: 346 93 6381
Fragments arranged by size:
6381 346 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2462 2869 2462
Size: 407 6413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2648 3033 3309 3354 3842 3951
Size: 399 458 400 385 276 45 488 109
Cuts at: 3951 4059 4139 4881 4966 6037 1391
Size: 108 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 458 400 399 385
276 109 108 85 80 45
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 3790 4709 6439 3790
Size: 919 1730 4171
Fragments arranged by size:
4171 1730 919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4175 844
Size: 3331 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3340 4707 1394
Size: 429 1517 1367 3507
Fragments arranged by size:
3507 1517 1367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2198 2397 3558 5315 5489 65
Size: 1183 950 199 1161 1757 174 1396
Fragments arranged by size:
1757 1396 1183 1161 950 199 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5464 5651 1700
Size: 110 3654 187 2869
Fragments arranged by size:
3654 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 4300
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 4880 5892 4880
Size: 1012 5808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3467 794
Size: 2673 4147
BstBI TT'CG_AA
Cuts at: 2764 2844 5624 2764
Size: 80 2780 3960
Fragments arranged by size:
3960 2780 80
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6820
BstXI CCAn_nnnn'nTGG
Cuts at: 2393 2782 3478 2393
Size: 389 696 5735
Fragments arranged by size:
5735 696 389
BstYI r'GATC_y
Cuts at: 1972 2560 4150 4517 4902 4919 6371 6457 6469
Size: 588 1590 367 385 17 1452 86 12
Cuts at: 6469 6815 1972
Size: 346 1977
Fragments arranged by size:
1977 1590 1452 588 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 4020 4020
Size: 6820
Bsu36I CC'TnA_GG
Cuts at: 2586 2586
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2652 3009 3123 513
Size: 552 1587 357 114 4210
Fragments arranged by size:
4210 1587 552 357 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3039 487
Size: 683 56 1813 4268
Fragments arranged by size:
4268 1813 683 56
BtsI GCAGTG_nn'
Cuts at: 2248 2409 2531 3381 4336 5130 5150 2248
Size: 161 122 850 955 794 20 3918
Fragments arranged by size:
3918 955 850 794 161 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6785
DraI TTT'AAA
Cuts at: 4124 4124
Size: 6820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5827 809
Size: 507 4511 1802
Fragments arranged by size:
4511 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3938 4267 5156
Size: 54 1425 50 275 18 2108 329 889
Cuts at: 5156 6785 8
Size: 1629 43
Fragments arranged by size:
2108 1629 1425 889 329 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4267 1537
Size: 275 2455 4090
Fragments arranged by size:
4090 2455 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3393 3502 4750 1353
Size: 2040 109 1248 3423
Fragments arranged by size:
3423 2040 1248 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5407 5781 5927 387
Size: 1064 138 3818 374 146 1280
Fragments arranged by size:
3818 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3352 3840 3352
Size: 488 6332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2219 2983 3441 4883 5480 6267
Size: 393 410 177 764 458 1442 597 787
Cuts at: 6267 6535 1239
Size: 268 1524
Fragments arranged by size:
1524 1442 787 764 597 458 410 393
268 177
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 3637 2585
Size: 1052 5768
EcoO109I rG'GnC_Cy
Cuts at: 2364 3168 3603 2364
Size: 804 435 5581
Fragments arranged by size:
5581 804 435
EcoRI G'AATT_C
Cuts at: 4042 4129 4507 4042
Size: 87 378 6355
Fragments arranged by size:
6355 378 87
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 3255 5967 1678
Size: 1577 2712 2531
Fragments arranged by size:
2712 2531 1577
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3071 3103 3387 3419 3760
Size: 32 135 32 1499 32 284 32 341
Cuts at: 3760 3792 5461 5493 5535 5567 1373
Size: 32 1669 32 42 32 2626
Fragments arranged by size:
2626 1669 1499 341 284 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2346 3130 4164 4410 669
Size: 988 281 408 784 1034 246 3079
Fragments arranged by size:
3079 1034 988 784 408 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 2346 4410 2346
Size: 2064 4756
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5934 6118 1305
Size: 4629 184 2007
Fragments arranged by size:
4629 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3940 10
Size: 54 1425 343 2108 2890
Fragments arranged by size:
2890 2108 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 2402 2657 3224 3476 3650 4778
Size: 648 1225 11 255 567 252 174 1128
Cuts at: 4778 5137 5296 518
Size: 359 159 2042
Fragments arranged by size:
2042 1225 1128 648 567 359 255 252
174 159 11
MspA1I CmG'CkG
Cuts at: 1606 3135 3611 3628 3936 4236 4913 6061 6306
Size: 1529 476 17 308 300 677 1148 245
Cuts at: 6306 1606
Size: 2120
Fragments arranged by size:
2120 1529 1148 677 476 308 300 245
17
NcoI C'CATG_G
Cuts at: 513 1065 3009 3123 513
Size: 552 1944 114 4210
Fragments arranged by size:
4210 1944 552 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NruI TCG'CGA
Cuts at: 3024 3024
Size: 6820
NsiI A_TGCA'T
Cuts at: 2010 2259 2010
Size: 249 6571
NspI r_CATG'y
Cuts at: 1132 1229 4018 4063 4178 5723 1132
Size: 97 2789 45 115 1545 2229
Fragments arranged by size:
2789 2229 1545 115 97 45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6820
PciI A'CATG_T
Cuts at: 4014 5719 4014
Size: 1705 5115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2953 3076 1108
Size: 1845 123 4852
Fragments arranged by size:
4852 1845 123
PfoI T'CCnGG_A
Cuts at: 3231 3872 3231
Size: 641 6179
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PmlI CAC'GTG
Cuts at: 3567 3963 3567
Size: 396 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2364 3168 3603 2364
Size: 804 435 5581
Fragments arranged by size:
5581 804 435
PshAI GACnn'nnGTC
Cuts at: 1613 3166 1613
Size: 1553 5267
PsiI TTA'TAA
Cuts at: 4390 4390
Size: 6820
PstI C_TGCA'G
Cuts at: 830 2733 3388 4172 830
Size: 1903 655 784 3478
Fragments arranged by size:
3478 1903 784 655
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 3611 3628 3611
Size: 17 6803
SacI G_AGCT'C
Cuts at: 3354 3842 3354
Size: 488 6332
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SanDI GG'GwC_CC
Cuts at: 2364 3603 2364
Size: 1239 5581
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 3901 5068 6780 3901
Size: 1167 1712 3941
Fragments arranged by size:
3941 1712 1167
SfcI C'TryA_G
Cuts at: 826 1045 2729 3384 3543 4104 4168 5303 5984
Size: 219 1684 655 159 561 64 1135 681
Cuts at: 5984 6175 826
Size: 191 1471
Fragments arranged by size:
1684 1471 1135 681 655 561 219 191
159 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 4147 1872
Size: 2275 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4924 5825 6087 820
Size: 197 926 2981 901 262 1553
Fragments arranged by size:
2981 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 4178 1132
Size: 3046 3774
SspI AAT'ATT
Cuts at: 5 52 2423 4744 5
Size: 47 2371 2321 2081
Fragments arranged by size:
2371 2321 2081 47
StuI AGG'CCT
Cuts at: 1517 4100 1517
Size: 2583 4237
StyI C'CwwG_G
Cuts at: 513 1065 3009 3123 4259 513
Size: 552 1944 114 1136 3074
Fragments arranged by size:
3074 1944 1136 552 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 3062 4847 4864 5017 5202 5647
Size: 1255 869 613 1785 17 153 185 445
Cuts at: 5647 325
Size: 1498
Fragments arranged by size:
1785 1498 1255 869 613 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 3525 3899
Size: 274 80 33 51 1443 639 909 374
Cuts at: 3899 5066 6778 96
Size: 1167 1712 138
Fragments arranged by size:
1712 1443 1167 909 639 374 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6780 6812 6780
Size: 32 6788
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6820
XbaI T'CTAG_A
Cuts at: 4156 4156
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3874 3874
Size: 6820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 4145 1870
Size: 2275 4545
XmnI GAAnn'nnTTC
Cuts at: 4949 4949
Size: 6820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV PacI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI