Restriction map for
pFN21AB7278
(Circular) (Six-base) MAPSORT of: pFN21AB7278.seq Check: 2037 from: 1 to: 6772
pFN21AB7278 6772 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2108 2108
Size: 6772
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4582 278
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2189 2226 4115 1937
Size: 252 37 1889 4594
Fragments arranged by size:
4594 1889 252 37
Acc65I G'GTAC_C
Cuts at: 4093 4093
Size: 6772
AclI AA'CG_TT
Cuts at: 1563 2491 4899 5272 1563
Size: 928 2408 373 3063
Fragments arranged by size:
3063 2408 928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4012 4835 6219 6487 1632
Size: 2380 823 1384 268 1917
Fragments arranged by size:
2380 1917 1384 823 268
AfeI AGC'GCT
Cuts at: 2557 2557
Size: 6772
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 5671 6648 1203
Size: 4468 977 1327
Fragments arranged by size:
4468 1327 977
AgeI A'CCGG_T
Cuts at: 3292 5603 3292
Size: 2311 4461
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 3389 1166
Size: 2223 4549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6772
AlwNI CAG_nnn'CTG
Cuts at: 2832 3562 4515 6087 2832
Size: 730 953 1572 3517
Fragments arranged by size:
3517 1572 953 730
ApaI G_GGCC'C
Cuts at: 3335 3605 3804 3335
Size: 270 199 6303
Fragments arranged by size:
6303 270 199
ApaLI G'TGCA_C
Cuts at: 3052 4829 5985 3052
Size: 1777 1156 3839
Fragments arranged by size:
3839 1777 1156
ApoI r'AATT_y
Cuts at: 1493 2948 4081 4307 4459 6568 1493
Size: 1455 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1455 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5326 160
Size: 872 4294 1606
Fragments arranged by size:
4294 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 2671 2817 3076 3131 3350 3572 4097
Size: 73 728 146 259 55 219 222 525
Cuts at: 4097 1870
Size: 4545
Fragments arranged by size:
4545 728 525 259 222 219 146 73
55
BamHI G'GATC_C
Cuts at: 4102 4469 4102
Size: 367 6405
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 2659 3299 3391 3440
Size: 325 217 627 810 62 640 92 49
Cuts at: 3440 3476 4093 5548 618
Size: 36 617 1455 1842
Fragments arranged by size:
1842 1455 810 640 627 617 325 217
92 62 49 36
BanII G_rGCy'C
Cuts at: 1391 3335 3605 3725 3761 3804 3972 4091 1391
Size: 1944 270 120 36 43 168 119 4072
Fragments arranged by size:
4072 1944 270 168 120 119 43 36
BbeI G_GCGC'C
Cuts at: 3303 3444 3480 3303
Size: 141 36 6595
Fragments arranged by size:
6595 141 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5801
BbvCI CC'TCA_GC
Cuts at: 2233 2852 2233
Size: 619 6153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3070 3104 3142 3176 4963 4997 1903
Size: 34 1133 34 38 34 1787 34 3678
Fragments arranged by size:
3678 1787 1133 38 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2005 4664 5874 2005
Size: 2659 1210 2903
Fragments arranged by size:
2903 2659 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6772
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3188 3346 5383
Size: 107 122 71 631 605 1516 158 2037
Cuts at: 5383 136
Size: 1525
Fragments arranged by size:
2037 1525 1516 631 605 158 122 107
71
BglII A'GATC_T
Cuts at: 6767 6767
Size: 6772
BlpI GC'TnA_GC
Cuts at: 4189 4189
Size: 6772
Bme1580I G_kGCm'C
Cuts at: 1790 2600 3056 3335 3605 3804 4010 4833 5989
Size: 810 456 279 270 199 206 823 1156
Cuts at: 5989 1790
Size: 2573
Fragments arranged by size:
2573 1156 823 810 456 279 270 206
199
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6772
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4503 5461 442
Size: 416 53 83 399 24 3086 958 1753
Fragments arranged by size:
3086 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3635 4033 5432 1239
Size: 2396 398 1399 2579
Fragments arranged by size:
2579 2396 1399 398
Bpu10I CC'TnA_GC
Cuts at: 2141 2233 2852 3714 2141
Size: 92 619 862 5199
Fragments arranged by size:
5199 862 619 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3986 4897 5762 6060 2168
Size: 1818 911 865 298 2880
Fragments arranged by size:
2880 1818 911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2264 5435 882
Size: 617 765 3171 2219
Fragments arranged by size:
3171 2219 765 617
BsaAI yAC'GTr
Cuts at: 493 1204 4563 493
Size: 711 3359 2702
Fragments arranged by size:
3359 2702 711
BsaBI GATnn'nnATC
Cuts at: 6766 6766
Size: 6772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3300 3441 3477 4579
Size: 53 83 186 936 1767 141 36 1102
Cuts at: 4579 4961 5596 275
Size: 382 635 1451
Fragments arranged by size:
1767 1451 1102 936 635 382 186 141
83 53 36
BsaWI w'CCGG_w
Cuts at: 3292 5205 5603 5877 6024 6435 3292
Size: 1913 398 274 147 411 3629
Fragments arranged by size:
3629 1913 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4031 4061 1178
Size: 30 165 30 2628 30 3889
Fragments arranged by size:
3889 2628 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2972 3218 3259 4007 1186
Size: 1204 582 246 41 748 3951
Fragments arranged by size:
3951 1204 748 582 246 41
BseYI C'CCAG_C
Cuts at: 2839 3341 3400 3550 3562 5975 6471 2839
Size: 502 59 150 12 2413 496 3140
Fragments arranged by size:
3140 2413 502 496 150 59 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6199
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3285 3795 4222 4983 6011
Size: 275 186 1108 176 510 427 761 1028
Cuts at: 6011 1540
Size: 2301
Fragments arranged by size:
2301 1108 1028 761 510 427 275 186
176
BsiHKAI G_wGCw'C
Cuts at: 3056 3761 4833 4918 5989 3056
Size: 705 1072 85 1071 3839
Fragments arranged by size:
3839 1072 1071 705 85
BsmI GAATG_Cn'
Cuts at: 2122 2486 4283 4376 2122
Size: 364 1797 93 4518
Fragments arranged by size:
4518 1797 364 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3128 3128
Size: 6772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2600 3056 3335 3605 3725 3761 3804
Size: 399 810 456 279 270 120 36 43
Cuts at: 3804 3972 4010 4091 4833 4918 5989 1391
Size: 168 38 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 810 742 456 399 279 270
168 120 85 81 43 38 36
BspEI T'CCGG_A
Cuts at: 6435 6435
Size: 6772
BspHI T'CATG_A
Cuts at: 4661 6391 4661
Size: 1730 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 4127 844
Size: 1264 2019 3489
Fragments arranged by size:
3489 2019 1264
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 3019 3696 4659 1394
Size: 429 526 670 677 963 3507
Fragments arranged by size:
3507 963 677 670 526 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3901 4024 5267 5441 65
Size: 1183 2653 123 1243 174 1396
Fragments arranged by size:
2653 1396 1243 1183 174 123
BsrFI r'CCGG_y
Cuts at: 1700 1810 2693 2753 3292 3345 3984 5416 5603
Size: 110 883 60 539 53 639 1432 187
Cuts at: 5603 1700
Size: 2869
Fragments arranged by size:
2869 1432 883 639 539 187 110 60
53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6772
BssHII G'CGCG_C
Cuts at: 1925 3567 1925
Size: 1642 5130
BssSI C'ACGA_G
Cuts at: 4832 5844 4832
Size: 1012 5760
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2832 794
Size: 2038 4734
BstBI TT'CG_AA
Cuts at: 5576 5576
Size: 6772
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6772
BstXI CCAn_nnnn'nTGG
Cuts at: 2806 3251 2806
Size: 445 6327
BstYI r'GATC_y
Cuts at: 1972 2435 3043 4102 4469 4854 4871 6323 6409
Size: 463 608 1059 367 385 17 1452 86
Cuts at: 6409 6421 6767 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1059 608 463 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2608 2922 3279 3320 3627 3660
Size: 552 939 604 314 357 41 307 33
Cuts at: 3660 3777 3795 513
Size: 117 18 3490
Fragments arranged by size:
3490 939 604 552 357 314 307 117
41 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2902 3977 487
Size: 683 56 1676 1075 3282
Fragments arranged by size:
3282 1676 1075 683 56
BtsI GCAGTG_nn'
Cuts at: 4288 5082 5102 4288
Size: 794 20 5958
Fragments arranged by size:
5958 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6737
DraI TTT'AAA
Cuts at: 4076 4076
Size: 6772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5779 809
Size: 507 4463 1802
Fragments arranged by size:
4463 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2796 2884
Size: 54 1425 50 275 18 456 510 88
Cuts at: 2884 3106 3189 3282 3792 4219 5108 6737 8
Size: 222 83 93 510 427 889 1629 43
Fragments arranged by size:
1629 1425 889 510 510 456 427 275
222 93 88 83 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 3282 3792 4219 1537
Size: 275 1294 176 510 427 4090
Fragments arranged by size:
4090 1294 510 427 275 176
EarI CTCTTCn'nnn_
Cuts at: 1353 2295 3093 3119 4702 1353
Size: 942 798 26 1583 3423
Fragments arranged by size:
3423 1583 942 798 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2912 2981 3447 5359 5733 5879
Size: 1064 138 1323 69 466 1912 374 146
Cuts at: 5879 387
Size: 1280
Fragments arranged by size:
1912 1323 1280 1064 466 374 146 138
69
EcoICRI GAG'CTC
Cuts at: 3759 3759
Size: 6772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3635 4012 4033 4835 5432 6219 6487
Size: 393 2003 377 21 802 597 787 268
Cuts at: 6487 1239
Size: 1524
Fragments arranged by size:
2003 1524 802 787 597 393 377 268
21
EcoNI CCTnn'n_nnAGG
Cuts at: 3200 3200
Size: 6772
EcoO109I rG'GnC_Cy
Cuts at: 2675 3331 3602 3684 3800 3801 2675
Size: 656 271 82 116 1 5646
Fragments arranged by size:
5646 656 271 116 82 1
EcoRI G'AATT_C
Cuts at: 4081 4459 4081
Size: 378 6394
FspI TGC'GCA
Cuts at: 5278 5278
Size: 6772
HaeII r_GCGC'y
Cuts at: 1678 2559 2985 3159 3303 3381 3444 3480 3504
Size: 881 426 174 144 78 63 36 24
Cuts at: 3504 3830 3985 4039 5919 1678
Size: 326 155 54 1880 2531
Fragments arranged by size:
2531 1880 881 426 326 174 155 144
78 63 54 36 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5413 5445 5487 5519 1373
Size: 32 135 32 3841 32 42 32 2626
Fragments arranged by size:
3841 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4116 4362 669
Size: 988 281 2178 246 3079
Fragments arranged by size:
3079 2178 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KasI G'GCGC_C
Cuts at: 3299 3440 3476 3299
Size: 141 36 6595
Fragments arranged by size:
6595 141 36
KpnI G_GTAC'C
Cuts at: 4097 4097
Size: 6772
MfeI C'AATT_G
Cuts at: 4371 4371
Size: 6772
MluI A'CGCG_T
Cuts at: 6648 6648
Size: 6772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2083 3465 3942 5886 6070 1305
Size: 778 1382 477 1944 184 2007
Fragments arranged by size:
2007 1944 1382 778 477 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2798 10
Size: 54 1425 343 966 3984
Fragments arranged by size:
3984 1425 966 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 4730 5089 5248 518
Size: 648 2223 1341 359 159 2042
Fragments arranged by size:
2223 2042 1341 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2338 2535 2747 2832 2859 2871 3281 3322
Size: 732 197 212 85 27 12 410 41
Cuts at: 3322 3337 3400 3423 3535 3544 3629 3662 3764
Size: 15 63 23 112 9 85 33 102
Cuts at: 3764 3770 3779 3797 3811 3817 4188 4865 6013
Size: 6 9 18 14 6 371 677 1148
Cuts at: 6013 6258 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 732 677 410 371 245 212
197 112 102 85 85 63 41 33
27 23 18 15 14 12 9 9
6 6
NaeI GCC'GGC
Cuts at: 2695 2755 3347 3986 2695
Size: 60 592 639 5481
Fragments arranged by size:
5481 639 592 60
NarI GG'CG_CC
Cuts at: 3300 3441 3477 3300
Size: 141 36 6595
Fragments arranged by size:
6595 141 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5281
Fragments arranged by size:
5281 939 552
NdeI CA'TA_TG
Cuts at: 387 3933 387
Size: 3546 3226
NgoMIV G'CCGG_C
Cuts at: 2693 2753 3345 3984 2693
Size: 60 592 639 5481
Fragments arranged by size:
5481 639 592 60
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 3282 3792 4219 3282
Size: 510 427 5835
Fragments arranged by size:
5835 510 427
NspI r_CATG'y
Cuts at: 1132 1229 2475 4130 5675 1132
Size: 97 1246 1655 1545 2229
Fragments arranged by size:
2229 1655 1545 1246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6772
PciI A'CATG_T
Cuts at: 5671 5671
Size: 6772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2794 3562 1108
Size: 1686 768 4318
Fragments arranged by size:
4318 1686 768
PfoI T'CCnGG_A
Cuts at: 2952 2952
Size: 6772
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4841 4873 6354 6386 4841
Size: 32 1481 32 5227
Fragments arranged by size:
5227 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2675 3684 2675
Size: 1009 5763
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PspOMI G'GGCC_C
Cuts at: 3331 3601 3800 3331
Size: 270 199 6303
Fragments arranged by size:
6303 270 199
PspXI vC'TCGA_Gb
Cuts at: 3350 3572 3350
Size: 222 6550
PstI C_TGCA'G
Cuts at: 830 3201 3775 3816 4124 830
Size: 2371 574 41 308 3478
Fragments arranged by size:
3478 2371 574 308 41
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
PvuII CAG'CTG
Cuts at: 2338 2832 3400 3535 3811 3817 2338
Size: 494 568 135 276 6 5293
Fragments arranged by size:
5293 568 494 276 135 6
SacI G_AGCT'C
Cuts at: 3761 3761
Size: 6772
SacII CC_GC'GG
Cuts at: 3282 3323 3630 3663 3780 3798 3282
Size: 41 307 33 117 18 6256
Fragments arranged by size:
6256 307 117 41 33 18
SalI G'TCGA_C
Cuts at: 1936 4114 1936
Size: 2178 4594
SanDI GG'GwC_CC
Cuts at: 2675 3684 2675
Size: 1009 5763
SapI GCTCTTCn'nnn_
Cuts at: 2295 2295
Size: 6772
SbfI CC_TGCA'GG
Cuts at: 3201 4124 3201
Size: 923 5849
ScaI AGT'ACT
Cuts at: 5020 6732 5020
Size: 1712 5060
SexAI A'CCwGG_T
Cuts at: 3921 3921
Size: 6772
SfcI C'TryA_G
Cuts at: 826 1045 3197 3771 3812 4120 5255 5936 6127
Size: 219 2152 574 41 308 1135 681 191
Cuts at: 6127 826
Size: 1471
Fragments arranged by size:
2152 1471 1135 681 574 308 219 191
41
SfoI GGC'GCC
Cuts at: 3301 3442 3478 3301
Size: 141 36 6595
Fragments arranged by size:
6595 141 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 2753 3292 3984 1700
Size: 1053 539 692 4488
Fragments arranged by size:
4488 1053 692 539
SmaI CCC'GGG
Cuts at: 1872 2673 2819 4099 1872
Size: 801 146 1280 4545
Fragments arranged by size:
4545 1280 801 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 3350 3572 4001 4876 5777
Size: 197 926 240 1167 222 429 875 901
Cuts at: 5777 6039 820
Size: 262 1553
Fragments arranged by size:
1553 1167 926 901 875 429 262 240
222 197
SnaBI TAC'GTA
Cuts at: 493 4563 493
Size: 4070 2702
SpeI A'CTAG_T
Cuts at: 152 2383 152
Size: 2231 4541
SphI G_CATG'C
Cuts at: 1132 4130 1132
Size: 2998 3774
SspI AAT'ATT
Cuts at: 5 52 4696 5
Size: 47 4644 2081
Fragments arranged by size:
4644 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6772
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2601 3036 3972 4211 513
Size: 552 939 45 552 435 936 239 3074
Fragments arranged by size:
3074 939 936 552 552 435 239 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4799 4816 4969 5154 5599 325
Size: 1255 3219 17 153 185 445 1498
Fragments arranged by size:
3219 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5018 6730 96
Size: 274 80 33 51 1443 3041 1712 138
Fragments arranged by size:
3041 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2699 2731 3035 3067 4031 4063 6732 6764 2699
Size: 32 304 32 964 32 2669 32 2707
Fragments arranged by size:
2707 2669 964 304 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6772
XbaI T'CTAG_A
Cuts at: 2432 4108 2432
Size: 1676 5096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3043
Size: 6772
XhoI C'TCGA_G
Cuts at: 1943 3350 3572 1943
Size: 1407 222 5143
Fragments arranged by size:
5143 1407 222
XmaI C'CCGG_G
Cuts at: 1870 2671 2817 4097 1870
Size: 801 146 1280 4545
Fragments arranged by size:
4545 1280 801 146
XmnI GAAnn'nnTTC
Cuts at: 4901 4901
Size: 6772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4580 276
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PsrI RsrII SfiI SrfI SwaI