Restriction map for
pFN21AB7277
(Circular) (Six-base) MAPSORT of: pFN21AB7277.seq Check: 9744 from: 1 to: 8569
pFN21AB7277 8569 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3801 6379 278
Size: 53 83 186 936 2265 2578 2468
Fragments arranged by size:
2578 2468 2265 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 5912 1937
Size: 211 3764 4594
Fragments arranged by size:
4594 3764 211
Acc65I G'GTAC_C
Cuts at: 3420 5890 3420
Size: 2470 6099
AclI AA'CG_TT
Cuts at: 1563 6696 7069 1563
Size: 5133 373 3063
Fragments arranged by size:
5133 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2404 3082 3448 4247 4333 4791 6632 8016
Size: 772 678 366 799 86 458 1841 1384
Cuts at: 8016 8284 1632
Size: 268 1917
Fragments arranged by size:
1917 1841 1384 799 772 678 458 366
268 86
AfeI AGC'GCT
Cuts at: 3761 4886 5046 3761
Size: 1125 160 7284
Fragments arranged by size:
7284 1125 160
AflII C'TTAA_G
Cuts at: 820 1017 4157 820
Size: 197 3140 5232
Fragments arranged by size:
5232 3140 197
AflIII A'CryG_T
Cuts at: 1203 3090 7468 8445 1203
Size: 1887 4378 977 1327
Fragments arranged by size:
4378 1887 1327 977
AgeI A'CCGG_T
Cuts at: 7400 7400
Size: 8569
AhdI GACnn_n'nnGTC
Cuts at: 4501 7298 4501
Size: 2797 5772
AleI CACnn'nnGTG
Cuts at: 1166 2403 3371 4016 1166
Size: 1237 968 645 5719
Fragments arranged by size:
5719 1237 968 645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8569
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4043 4075 4043
Size: 32 8537
AlwNI CAG_nnn'CTG
Cuts at: 2052 2479 2779 4072 4270 4702 5038 5179 5522
Size: 427 300 1293 198 432 336 141 343
Cuts at: 5522 5572 5596 5665 5686 6312 7884 2052
Size: 50 24 69 21 626 1572 2737
Fragments arranged by size:
2737 1572 1293 626 432 427 343 336
300 198 141 69 50 24 21
ApaI G_GGCC'C
Cuts at: 4472 5092 5209 5480 4472
Size: 620 117 271 7561
Fragments arranged by size:
7561 620 271 117
ApaLI G'TGCA_C
Cuts at: 4307 6626 7782 4307
Size: 2319 1156 5094
Fragments arranged by size:
5094 2319 1156
ApoI r'AATT_y
Cuts at: 1493 5878 6104 6256 8365 1493
Size: 4385 226 152 2109 1697
Fragments arranged by size:
4385 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7123 160
Size: 872 6091 1606
Fragments arranged by size:
6091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8569
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2160 2174 2180 2187 3103 3626
Size: 73 164 53 14 6 7 916 523
Cuts at: 3626 4559 4925 5132 5468 5763 5894 1870
Size: 933 366 207 336 295 131 4545
Fragments arranged by size:
4545 933 916 523 366 336 295 207
164 131 73 53 14 7 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5546 5579 5546
Size: 33 8536
BamHI G'GATC_C
Cuts at: 5843 5899 6266 5843
Size: 56 367 8146
Fragments arranged by size:
8146 367 56
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 3049 3123 3420 3883
Size: 325 217 627 314 948 74 297 463
Cuts at: 3883 4379 4852 4904 4921 5702 5890 7345 618
Size: 496 473 52 17 781 188 1455 1842
Fragments arranged by size:
1842 1455 948 781 627 496 473 463
325 314 297 217 188 74 52 17
BanII G_rGCy'C
Cuts at: 1391 2181 2825 3044 3174 3249 3346 3483 4136
Size: 790 644 219 130 75 97 137 653
Cuts at: 4136 4472 4560 4568 5092 5209 5438 5453 5469
Size: 336 88 8 524 117 229 15 16
Cuts at: 5469 5480 5587 5888 1391
Size: 11 107 301 4072
Fragments arranged by size:
4072 790 653 644 524 336 301 229
219 137 130 117 107 97 88 75
16 15 11 8
BbeI G_GCGC'C
Cuts at: 2105 3053 3887 2105
Size: 948 834 6787
Fragments arranged by size:
6787 948 834
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7598
BbvCI CC'TCA_GC
Cuts at: 5201 5201
Size: 8569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4916 4931 4950 4965 6760 6794 1903
Size: 34 2979 15 19 15 1795 34 3678
Fragments arranged by size:
3678 2979 1795 34 34 19 15 15
BciVI GTATCCnnnnn_n'
Cuts at: 6461 7671 6461
Size: 1210 7359
BclI T'GATC_A
Cuts at: 1548 4338 1548
Size: 2790 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4149 5015 5828
Size: 107 122 71 631 605 2477 866 813
Cuts at: 5828 7180 136
Size: 1352 1525
Fragments arranged by size:
2477 1525 1352 866 813 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4161 8564 4161
Size: 4403 4166
BlpI GC'TnA_GC
Cuts at: 2078 2138 2819 4135 4976 5327 5501 5986 2078
Size: 60 681 1316 841 351 174 485 4661
Fragments arranged by size:
4661 1316 841 681 485 351 174 60
Bme1580I G_kGCm'C
Cuts at: 1790 3126 3508 3565 3828 3977 4311 4472 4847
Size: 1336 382 57 263 149 334 161 375
Cuts at: 4847 4907 4926 5092 5140 5209 5480 6630 7786
Size: 60 19 166 48 69 271 1150 1156
Cuts at: 7786 1790
Size: 2573
Fragments arranged by size:
2573 1336 1156 1150 382 375 334 271
263 166 161 149 69 60 57 48
19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3328 3641 3710
Size: 416 53 83 399 24 1911 313 69
Cuts at: 3710 4517 4819 6300 7258 442
Size: 807 302 1481 958 1753
Fragments arranged by size:
1911 1753 1481 958 807 416 399 313
302 83 69 53 24
BmtI G_CTAG'C
Cuts at: 1056 4143 1056
Size: 3087 5482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5404 5789 5404
Size: 385 8184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2378 2640 2696 3507 3614 3685 3695
Size: 1082 57 262 56 811 107 71 10
Cuts at: 3695 4203 5836 7229 1239
Size: 508 1633 1393 2579
Fragments arranged by size:
2579 1633 1393 1082 811 508 262 107
71 57 56 10
Bpu10I CC'TnA_GC
Cuts at: 3665 3792 5201 3665
Size: 127 1409 7033
Fragments arranged by size:
7033 1409 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3056 4346 5530 6694 7559 7857 3056
Size: 1290 1184 1164 865 298 3768
Fragments arranged by size:
3768 1290 1184 1164 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2708 3434 3677 4412 5392 7232 882
Size: 617 1209 726 243 735 980 1840 2219
Fragments arranged by size:
2219 1840 1209 980 735 726 617 243
BsaAI yAC'GTr
Cuts at: 493 1204 2152 3978 6360 493
Size: 711 948 1826 2382 2702
Fragments arranged by size:
2702 2382 1826 948 711
BsaBI GATnn'nnATC
Cuts at: 8563 8563
Size: 8569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 3050 3798 3884
Size: 53 83 186 936 569 948 748 86
Cuts at: 3884 4099 6376 6758 7393 275
Size: 215 2277 382 635 1451
Fragments arranged by size:
2277 1451 948 936 748 635 569 382
215 186 86 83 53
BsaWI w'CCGG_w
Cuts at: 2973 3871 4957 5365 5458 7002 7400 7674 7821
Size: 898 1086 408 93 1544 398 274 147
Cuts at: 7821 8232 2973
Size: 411 3310
Fragments arranged by size:
3310 1544 1086 898 411 408 398 274
147 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3683 3713 4937 4967 5787
Size: 30 165 30 2280 30 1224 30 820
Cuts at: 5787 5817 1178
Size: 30 3930
Fragments arranged by size:
3930 2280 1224 820 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 3197 3908 4643 4943 4958 4976 5417
Size: 1246 765 711 735 300 15 18 441
Cuts at: 5417 5475 1186
Size: 58 4280
Fragments arranged by size:
4280 1246 765 735 711 441 300 58
18 15
BseYI C'CCAG_C
Cuts at: 3119 3173 3268 3815 3964 4619 4705 4748 4871
Size: 54 95 547 149 655 86 43 123
Cuts at: 4871 5427 7772 8268 3119
Size: 556 2345 496 3420
Fragments arranged by size:
3420 2345 655 556 547 496 149 123
95 86 54 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2885 4498 4970 5045 5216 5516 5597
Size: 573 1149 1613 472 75 171 300 81
Cuts at: 5597 5690 1163
Size: 93 4042
Fragments arranged by size:
4042 1613 1149 573 472 300 171 93
81 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 2258 2264 4006 4211 4635
Size: 275 186 134 123 6 1742 205 424
Cuts at: 4635 4941 6019 6780 7808 1540
Size: 306 1078 761 1028 2301
Fragments arranged by size:
2301 1742 1078 1028 761 424 306 275
205 186 134 123 6
BsiHKAI G_wGCw'C
Cuts at: 2442 3207 3249 4244 4311 4560 6630 6715 7786
Size: 765 42 995 67 249 2070 85 1071
Cuts at: 7786 2442
Size: 3225
Fragments arranged by size:
3225 2070 1071 995 765 249 85 67
42
BsmI GAATG_Cn'
Cuts at: 6080 6173 6080
Size: 93 8476
BsmBI CGTCTCn'nnnn_
Cuts at: 2891 3938 4959 2891
Size: 1047 1021 6501
Fragments arranged by size:
6501 1047 1021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2442 2825 3044 3126 3174 3207
Size: 399 391 261 383 219 82 48 33
Cuts at: 3207 3249 3346 3483 3508 3565 3828 3977 4136
Size: 42 97 137 25 57 263 149 159
Cuts at: 4136 4244 4311 4472 4560 4568 4847 4907 4926
Size: 108 67 161 88 8 279 60 19
Cuts at: 4926 5092 5140 5209 5438 5453 5469 5480 5587
Size: 166 48 69 229 15 16 11 107
Cuts at: 5587 5888 6630 6715 7786 1391
Size: 301 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 383 301 279
263 261 229 219 166 161 159 149
137 108 107 97 88 85 82 69
67 60 57 48 48 42 33 25
19 16 15 11 8
BspEI T'CCGG_A
Cuts at: 4957 8232 4957
Size: 3275 5294
BspHI T'CATG_A
Cuts at: 2835 6458 8188 2835
Size: 3623 1730 3216
Fragments arranged by size:
3623 3216 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 2478 4047 5924 844
Size: 1392 242 1569 1877 3489
Fragments arranged by size:
3489 1877 1569 1392 242
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 2749 3955 4048 5483 6456 1394
Size: 429 314 612 1206 93 1435 973 3507
Fragments arranged by size:
3507 1435 1206 973 612 429 314 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 3210 7064 7238 65
Size: 1183 1962 3854 174 1396
Fragments arranged by size:
3854 1962 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2088 2130 2658 5160 7213 7400
Size: 110 232 46 42 528 2502 2053 187
Cuts at: 7400 1700
Size: 2869
Fragments arranged by size:
2869 2502 2053 528 232 187 110 46
42
BsrGI T'GTAC_A
Cuts at: 96 4596 96
Size: 4500 4069
BssHII G'CGCG_C
Cuts at: 1925 2038 2121 2241 1925
Size: 113 83 120 8253
Fragments arranged by size:
8253 120 113 83
BssSI C'ACGA_G
Cuts at: 4012 4553 6629 7641 4012
Size: 541 2076 1012 4940
Fragments arranged by size:
4940 2076 1012 541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2479 2911 3294 5038 794
Size: 1685 432 383 1744 4325
Fragments arranged by size:
4325 1744 1685 432 383
BstBI TT'CG_AA
Cuts at: 7373 7373
Size: 8569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8569
BstXI CCAn_nnnn'nTGG
Cuts at: 3119 3373 4393 5216 3119
Size: 254 1020 823 6472
Fragments arranged by size:
6472 1020 823 254
BstYI r'GATC_y
Cuts at: 1972 3348 4038 4161 4266 5271 5289 5843 5899
Size: 1376 690 123 105 1005 18 554 56
Cuts at: 5899 6266 6651 6668 8120 8206 8218 8564 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1376 1005 690 554 385 367
346 123 105 86 56 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 4848 4848
Size: 8569
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2258 2316 3879 3888 4769
Size: 552 939 66 188 58 1563 9 881
Cuts at: 4769 513
Size: 4313
Fragments arranged by size:
4313 1563 939 881 552 188 66 58
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2874 4009 487
Size: 683 56 1648 1135 5047
Fragments arranged by size:
5047 1648 1135 683 56
BtsI GCAGTG_nn'
Cuts at: 3826 4700 5321 5678 6085 6879 6899 3826
Size: 874 621 357 407 794 20 5496
Fragments arranged by size:
5496 874 794 621 407 357 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8534
DraI TTT'AAA
Cuts at: 5873 5873
Size: 8569
DraIII CAC_nnn'GTG
Cuts at: 3503 4306 3503
Size: 803 7766
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7576 809
Size: 507 6260 1802
Fragments arranged by size:
6260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2132 2255
Size: 54 1425 50 275 18 237 65 123
Cuts at: 2255 2261 2550 2877 2928 3073 4530 4632 4938
Size: 6 289 327 51 145 1457 102 306
Cuts at: 4938 5016 6016 6905 8534 8
Size: 78 1000 889 1629 43
Fragments arranged by size:
1629 1457 1425 1000 889 327 306 289
275 237 145 123 102 78 65 54
51 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2132 2255 2261 4632 4938 6016 1537
Size: 275 320 123 6 2371 306 1078 4090
Fragments arranged by size:
4090 2371 1078 320 306 275 123 6
EarI CTCTTCn'nnn_
Cuts at: 1353 4074 4095 5109 6499 1353
Size: 2721 21 1014 1390 3423
Fragments arranged by size:
3423 2721 1390 1014 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 2219 3914 4599 5066 7156
Size: 1064 138 459 171 1695 685 467 2090
Cuts at: 7156 7530 7676 387
Size: 374 146 1280
Fragments arranged by size:
2090 1695 1280 1064 685 467 459 374
171 146 138
EcoICRI GAG'CTC
Cuts at: 3247 4558 3247
Size: 1311 7258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2378 2404 2640 2696 3082 3448
Size: 393 689 57 26 236 56 386 366
Cuts at: 3448 3507 3614 3685 3695 4203 4247 4333 4791
Size: 59 107 71 10 508 44 86 458
Cuts at: 4791 5836 6632 7229 8016 8284 1239
Size: 1045 796 597 787 268 1524
Fragments arranged by size:
1524 1045 796 787 689 597 508 458
393 386 366 268 236 107 86 71
59 57 56 44 26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 2483 2675 3851 2231
Size: 252 192 1176 6949
Fragments arranged by size:
6949 1176 252 192
EcoO109I rG'GnC_Cy
Cuts at: 2059 2248 2476 2593 2635 3004 4220 4468 4711
Size: 189 228 117 42 369 1216 248 243
Cuts at: 4711 5088 5205 5212 5476 5477 5569 5662 2059
Size: 377 117 7 264 1 92 93 4966
Fragments arranged by size:
4966 1216 377 369 264 248 243 228
189 117 117 93 92 42 7 1
EcoRI G'AATT_C
Cuts at: 5878 6256 5878
Size: 378 8191
FspI TGC'GCA
Cuts at: 2279 4022 7075 2279
Size: 1743 3053 3773
Fragments arranged by size:
3773 3053 1743
FspAI rTGC'GCAy
Cuts at: 4022 4022
Size: 8569
HaeII r_GCGC'y
Cuts at: 1678 2078 2105 3053 3744 3763 3887 4695 4888
Size: 400 27 948 691 19 124 808 193
Cuts at: 4888 5048 5189 5287 7716 1678
Size: 160 141 98 2429 2531
Fragments arranged by size:
2531 2429 948 808 691 400 193 160
141 124 98 27 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3333 3365 3683 3715 4487
Size: 32 135 32 1761 32 318 32 772
Cuts at: 4487 4519 4937 4969 4987 5019 7210 7242 7284
Size: 32 418 32 18 32 2191 32 42
Cuts at: 7284 7316 1373
Size: 32 2626
Fragments arranged by size:
2626 2191 1761 772 418 318 135 42
32 32 32 32 32 32 32 32
32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 5913 6159 669
Size: 988 281 3975 246 3079
Fragments arranged by size:
3975 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8569
HpaI GTT'AAC
Cuts at: 6159 6159
Size: 8569
KasI G'GCGC_C
Cuts at: 2101 3049 3883 2101
Size: 948 834 6787
Fragments arranged by size:
6787 948 834
KpnI G_GTAC'C
Cuts at: 3424 5894 3424
Size: 2470 6099
MfeI C'AATT_G
Cuts at: 6168 6168
Size: 8569
MluI A'CGCG_T
Cuts at: 8445 8445
Size: 8569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3900 5663 7683 7867 1305
Size: 2595 1763 2020 184 2007
Fragments arranged by size:
2595 2020 2007 1763 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4532 10
Size: 54 1425 343 2700 4047
Fragments arranged by size:
4047 2700 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2403 2834 3371 4016 4526 4840 5711
Size: 648 1237 431 537 645 510 314 871
Cuts at: 5711 6527 6886 7045 518
Size: 816 359 159 2042
Fragments arranged by size:
2042 1237 871 816 648 645 537 510
431 359 314 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2065 2212 2260 2275 2318 2767 2794
Size: 443 16 147 48 15 43 449 27
Cuts at: 2794 3025 3200 3394 3705 3805 3890 4493 5242
Size: 231 175 194 311 100 85 603 749
Cuts at: 5242 5500 5581 5593 5985 6662 7810 8055 1606
Size: 258 81 12 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 603 449 443 392
311 258 245 231 194 175 147 100
85 81 48 43 27 16 15 12
NaeI GCC'GGC
Cuts at: 2044 2090 2132 2660 5162 2044
Size: 46 42 528 2502 5451
Fragments arranged by size:
5451 2502 528 46 42
NarI GG'CG_CC
Cuts at: 2102 3050 3884 2102
Size: 948 834 6787
Fragments arranged by size:
6787 948 834
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7078
Fragments arranged by size:
7078 939 552
NdeI CA'TA_TG
Cuts at: 387 3416 3728 387
Size: 3029 312 5228
Fragments arranged by size:
5228 3029 312
NgoMIV G'CCGG_C
Cuts at: 2042 2088 2130 2658 5160 2042
Size: 46 42 528 2502 5451
Fragments arranged by size:
5451 2502 528 46 42
NheI G'CTAG_C
Cuts at: 1052 4139 1052
Size: 3087 5482
NotI GC'GGCC_GC
Cuts at: 2261 6016 2261
Size: 3755 4814
NspI r_CATG'y
Cuts at: 1132 1229 3070 3094 4766 5927 7472 1132
Size: 97 1841 24 1672 1161 1545 2229
Fragments arranged by size:
2229 1841 1672 1545 1161 97 24
PasI CC'CwG_GG
Cuts at: 1348 3565 5209 5350 1348
Size: 2217 1644 141 4567
Fragments arranged by size:
4567 2217 1644 141
PciI A'CATG_T
Cuts at: 3090 7468 3090
Size: 4378 4191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3580 5572 5665 1108
Size: 2472 1992 93 4012
Fragments arranged by size:
4012 2472 1992 93
PfoI T'CCnGG_A
Cuts at: 2709 3111 3591 4041 5253 2709
Size: 402 480 450 1212 6025
Fragments arranged by size:
6025 1212 480 450 402
PmeI GTTT'AAAC
Cuts at: 5873 5873
Size: 8569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6638 6670 8151 8183 6638
Size: 32 1481 32 7024
Fragments arranged by size:
7024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2248 4220 4711 2059
Size: 189 1972 491 5917
Fragments arranged by size:
5917 1972 491 189
PshAI GACnn'nnGTC
Cuts at: 1613 5618 1613
Size: 4005 4564
PsiI TTA'TAA
Cuts at: 6139 6139
Size: 8569
PspOMI G'GGCC_C
Cuts at: 4468 5088 5205 5476 4468
Size: 620 117 271 7561
Fragments arranged by size:
7561 620 271 117
PspXI vC'TCGA_Gb
Cuts at: 2180 3626 2180
Size: 1446 7123
PstI C_TGCA'G
Cuts at: 830 2475 2793 2907 3388 4275 4440 4725 5921
Size: 1645 318 114 481 887 165 285 1196
Cuts at: 5921 830
Size: 3478
Fragments arranged by size:
3478 1645 1196 887 481 318 285 165
114
PvuI CG_AT'CG
Cuts at: 2001 4006 2001
Size: 2005 6564
PvuII CAG'CTG
Cuts at: 2049 2275 2767 3200 3394 3705 3805 4493 5500
Size: 226 492 433 194 311 100 688 1007
Cuts at: 5500 2049
Size: 5118
Fragments arranged by size:
5118 1007 688 492 433 311 226 194
100
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 8569
SacI G_AGCT'C
Cuts at: 3249 4560 3249
Size: 1311 7258
SacII CC_GC'GG
Cuts at: 2261 2319 3891 2261
Size: 58 1572 6939
Fragments arranged by size:
6939 1572 58
SalI G'TCGA_C
Cuts at: 1936 5911 1936
Size: 3975 4594
SanDI GG'GwC_CC
Cuts at: 4220 4711 4220
Size: 491 8078
SapI GCTCTTCn'nnn_
Cuts at: 4095 5109 4095
Size: 1014 7555
SbfI CC_TGCA'GG
Cuts at: 2475 4275 5921 2475
Size: 1800 1646 5123
Fragments arranged by size:
5123 1800 1646
ScaI AGT'ACT
Cuts at: 6817 8529 6817
Size: 1712 6857
SexAI A'CCwGG_T
Cuts at: 3423 3423
Size: 8569
SfcI C'TryA_G
Cuts at: 826 1045 2471 2789 2903 3384 3530 4271 4436
Size: 219 1426 318 114 481 146 741 165
Cuts at: 4436 4721 5588 5678 5917 7052 7733 7924 826
Size: 285 867 90 239 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 867 741 681 481 318
285 239 219 191 165 146 114 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 5015 5015
Size: 8569
SfoI GGC'GCC
Cuts at: 2103 3051 3885 2103
Size: 948 834 6787
Fragments arranged by size:
6787 948 834
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8569
SmaI CCC'GGG
Cuts at: 1872 2109 2162 2176 5896 1872
Size: 237 53 14 3720 4545
Fragments arranged by size:
4545 3720 237 53 14
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3035 3626 4157 4361 5509
Size: 197 926 237 855 591 531 204 1148
Cuts at: 5509 5763 6673 7574 7836 820
Size: 254 910 901 262 1553
Fragments arranged by size:
1553 1148 926 910 901 855 591 531
262 254 237 204 197
SnaBI TAC'GTA
Cuts at: 493 6360 493
Size: 5867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8569
SphI G_CATG'C
Cuts at: 1132 5927 1132
Size: 4795 3774
SrfI GCCC'GGGC
Cuts at: 2176 2176
Size: 8569
SspI AAT'ATT
Cuts at: 5 52 6493 5
Size: 47 6441 2081
Fragments arranged by size:
6441 2081 47
StuI AGG'CCT
Cuts at: 1517 2583 2909 2986 3137 3857 1517
Size: 1066 326 77 151 720 6229
Fragments arranged by size:
6229 1066 720 326 151 77
StyI C'CwwG_G
Cuts at: 513 1065 2004 2401 5215 6008 513
Size: 552 939 397 2814 793 3074
Fragments arranged by size:
3074 2814 939 793 552 397
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2645 2701 3383 3850 4838 4881 4885
Size: 1255 1065 56 682 467 988 43 4
Cuts at: 4885 5342 6596 6613 6766 6951 7396 325
Size: 457 1254 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1254 1065 988 682 467 457
445 185 153 56 43 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3060 3936 4596
Size: 274 80 33 51 1443 1083 876 660
Cuts at: 4596 6815 8527 96
Size: 2219 1712 138
Fragments arranged by size:
2219 1712 1443 1083 876 660 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5355 5387 8529 8561 5355
Size: 32 3142 32 5363
Fragments arranged by size:
5363 3142 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8569
XbaI T'CTAG_A
Cuts at: 5905 5905
Size: 8569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2887 2923 3421 4585 2887
Size: 36 498 1164 6871
Fragments arranged by size:
6871 1164 498 36
XhoI C'TCGA_G
Cuts at: 1943 2180 3626 5763 1943
Size: 237 1446 2137 4749
Fragments arranged by size:
4749 2137 1446 237
XmaI C'CCGG_G
Cuts at: 1870 2107 2160 2174 5894 1870
Size: 237 53 14 3720 4545
Fragments arranged by size:
4545 3720 237 53 14
XmnI GAAnn'nnTTC
Cuts at: 6698 6698
Size: 8569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3799 6377 276
Size: 53 83 186 936 2265 2578 2468
Fragments arranged by size:
2578 2468 2265 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FalI
FseI NruI NsiI PacI PmlI PsrI SwaI