Restriction map for
pFN21AB7275
(Circular) (Six-base) MAPSORT of: pFN21AB7275.seq Check: 3437 from: 1 to: 7330
pFN21AB7275 7330 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2984 2984
Size: 7330
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5140 278
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4673 1937
Size: 2736 4594
Acc65I G'GTAC_C
Cuts at: 4147 4651 4147
Size: 504 6826
AclI AA'CG_TT
Cuts at: 1563 5457 5830 1563
Size: 3894 373 3063
Fragments arranged by size:
3894 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3092 3546 3653 4482 5393 6777 7045 1632
Size: 1460 454 107 829 911 1384 268 1917
Fragments arranged by size:
1917 1460 1384 911 829 454 268 107
AflII C'TTAA_G
Cuts at: 820 1017 2057 820
Size: 197 1040 6093
Fragments arranged by size:
6093 1040 197
AflIII A'CryG_T
Cuts at: 1203 2570 3195 6229 7206 1203
Size: 1367 625 3034 977 1327
Fragments arranged by size:
3034 1367 1327 977 625
AgeI A'CCGG_T
Cuts at: 6161 6161
Size: 7330
AhdI GACnn_n'nnGTC
Cuts at: 6059 6059
Size: 7330
AleI CACnn'nnGTG
Cuts at: 1166 2270 3093 1166
Size: 1104 823 5403
Fragments arranged by size:
5403 1104 823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7330
AlwNI CAG_nnn'CTG
Cuts at: 3406 5073 6645 3406
Size: 1667 1572 4091
Fragments arranged by size:
4091 1667 1572
ApaLI G'TGCA_C
Cuts at: 5387 6543 5387
Size: 1156 6174
ApoI r'AATT_y
Cuts at: 1493 2303 2577 2710 3082 3823 4163 4584 4639
Size: 810 274 133 372 741 340 421 55
Cuts at: 4639 4865 5017 7126 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 810 741 421 372 340 274
226 152 133 55
AseI AT'TA_AT
Cuts at: 160 1032 5884 160
Size: 872 4852 1606
Fragments arranged by size:
4852 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
AvaI C'yCGr_G
Cuts at: 1870 1943 4515 4655 1870
Size: 73 2572 140 4545
Fragments arranged by size:
4545 2572 140 73
AvrII C'CTAG_G
Cuts at: 3842 3842
Size: 7330
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4139 4172 4139
Size: 33 7297
BamHI G'GATC_C
Cuts at: 4660 5027 4660
Size: 367 6963
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3637 4147 4651 6106 618
Size: 325 217 627 1850 510 504 1455 1842
Fragments arranged by size:
1850 1842 1455 627 510 504 325 217
BanII G_rGCy'C
Cuts at: 1391 3229 4649 1391
Size: 1838 1420 4072
Fragments arranged by size:
4072 1838 1420
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 3527 928
Size: 971 369 1259 4731
Fragments arranged by size:
4731 1259 971 369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2226 2260 3191 3225 5521 5555 1903
Size: 34 289 34 931 34 2296 34 3678
Fragments arranged by size:
3678 2296 931 289 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2530 2616 2664 5222 6432 2530
Size: 86 48 2558 1210 3428
Fragments arranged by size:
3428 2558 1210 86 48
BclI T'GATC_A
Cuts at: 1548 2103 3301 1548
Size: 555 1198 5577
Fragments arranged by size:
5577 1198 555
BfrBI ATG'CAT
Cuts at: 2186 2186
Size: 7330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5941 136
Size: 107 122 71 631 605 4269 1525
Fragments arranged by size:
4269 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7325 7325
Size: 7330
BlpI GC'TnA_GC
Cuts at: 4747 4747
Size: 7330
Bme1580I G_kGCm'C
Cuts at: 1790 2462 5391 6547 1790
Size: 672 2929 1156 2573
Fragments arranged by size:
2929 2573 1156 672
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 3946 4551
Size: 416 53 83 399 24 2084 445 605
Cuts at: 4551 5061 6019 442
Size: 510 958 1753
Fragments arranged by size:
2084 1753 958 605 510 445 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 4272 5990 1239
Size: 983 2050 1718 2579
Fragments arranged by size:
2579 2050 1718 983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5455 6320 6618 5455
Size: 865 298 6167
Fragments arranged by size:
6167 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 5993 882
Size: 617 813 3681 2219
Fragments arranged by size:
3681 2219 813 617
BsaAI yAC'GTr
Cuts at: 493 1204 5121 493
Size: 711 3917 2702
Fragments arranged by size:
3917 2702 711
BsaBI GATnn'nnATC
Cuts at: 2697 7324 2697
Size: 4627 2703
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5137 5519 6154 275
Size: 53 83 186 936 3604 382 635 1451
Fragments arranged by size:
3604 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4384 5763 6161 6435 6582 6993 4384
Size: 1379 398 274 147 411 4721
Fragments arranged by size:
4721 1379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4221 4251 1178
Size: 30 165 30 2818 30 4257
Fragments arranged by size:
4257 2818 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3456 3680 1186
Size: 2270 224 4836
Fragments arranged by size:
4836 2270 224
BseYI C'CCAG_C
Cuts at: 2250 2445 6533 7029 2250
Size: 195 4088 496 2551
Fragments arranged by size:
4088 2551 496 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3332 4589 1163
Size: 573 1596 1257 3904
Fragments arranged by size:
3904 1596 1257 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4780 5541 6569 1540
Size: 275 186 2779 761 1028 2301
Fragments arranged by size:
2779 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3229 5391 5476 6547 3229
Size: 2162 85 1071 4012
Fragments arranged by size:
4012 2162 1071 85
BsmI GAATG_Cn'
Cuts at: 3582 4841 4934 3582
Size: 1259 93 5978
Fragments arranged by size:
5978 1259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2462 3229 4649 5391 5476 6547 1391
Size: 399 672 767 1420 742 85 1071 2174
Fragments arranged by size:
2174 1420 1071 767 742 672 399 85
BspEI T'CCGG_A
Cuts at: 6993 6993
Size: 7330
BspHI T'CATG_A
Cuts at: 3241 5219 6949 3241
Size: 1978 1730 3622
Fragments arranged by size:
3622 1978 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2484 2934 2984 4685 844
Size: 1640 450 50 1701 3489
Fragments arranged by size:
3489 1701 1640 450 50
BsrBI CCG'CTC
Cuts at: 1394 1823 5217 1394
Size: 429 3394 3507
Fragments arranged by size:
3507 3394 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 3704 4509 5825 5999 65
Size: 1183 1539 917 805 1316 174 1396
Fragments arranged by size:
1539 1396 1316 1183 917 805 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2211 5974 6161 1700
Size: 110 401 3763 187 2869
Fragments arranged by size:
3763 2869 401 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7330
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7330
BssSI C'ACGA_G
Cuts at: 5390 6402 5390
Size: 1012 6318
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7330
BstBI TT'CG_AA
Cuts at: 6134 6134
Size: 7330
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7330
BstXI CCAn_nnnn'nTGG
Cuts at: 2751 2751
Size: 7330
BstYI r'GATC_y
Cuts at: 1972 4660 5027 5412 5429 6881 6967 6979 7325
Size: 2688 367 385 17 1452 86 12 346
Cuts at: 7325 1972
Size: 1977
Fragments arranged by size:
2688 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3121 3451 3610 4072 513
Size: 552 939 1117 330 159 462 3771
Fragments arranged by size:
3771 1117 939 552 462 330 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3951 487
Size: 683 56 2725 3866
Fragments arranged by size:
3866 2725 683 56
BtsI GCAGTG_nn'
Cuts at: 4846 5640 5660 4846
Size: 794 20 6516
Fragments arranged by size:
6516 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2750 2785 529
Size: 35 2186 35 5074
Fragments arranged by size:
5074 2186 35 35
DraI TTT'AAA
Cuts at: 2796 3514 4634 2796
Size: 718 1120 5492
Fragments arranged by size:
5492 1120 718
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6337 809
Size: 507 5021 1802
Fragments arranged by size:
5021 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4273 4777 5666
Size: 54 1425 50 275 18 2443 504 889
Cuts at: 5666 7295 8
Size: 1629 43
Fragments arranged by size:
2443 1629 1425 889 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4777 1537
Size: 275 2965 4090
Fragments arranged by size:
4090 2965 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2886 5260 1353
Size: 1533 2374 3423
Fragments arranged by size:
3423 2374 1533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5917 6291 6437 387
Size: 1064 138 4328 374 146 1280
Fragments arranged by size:
4328 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3227 3227
Size: 7330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 3092 3546 3653 4272 4482 5393
Size: 393 590 870 454 107 619 210 911
Cuts at: 5393 5990 6777 7045 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 911 870 787 619 597 590 454
393 268 210 107
EcoO109I rG'GnC_Cy
Cuts at: 3206 3206
Size: 7330
EcoRI G'AATT_C
Cuts at: 2577 4163 4639 5017 2577
Size: 1586 476 378 4890
Fragments arranged by size:
4890 1586 476 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 7330
FspI TGC'GCA
Cuts at: 5836 5836
Size: 7330
HaeII r_GCGC'y
Cuts at: 1678 6477 1678
Size: 4799 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3656 3688 3769 3801 5971
Size: 32 135 32 2084 32 81 32 2170
Cuts at: 5971 6003 6045 6077 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2170 2084 135 81 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2019 4674 4920 669
Size: 988 281 81 2655 246 3079
Fragments arranged by size:
3079 2655 988 281 246 81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7330
HpaI GTT'AAC
Cuts at: 4920 4920
Size: 7330
KpnI G_GTAC'C
Cuts at: 4151 4655 4151
Size: 504 6826
MfeI C'AATT_G
Cuts at: 4929 4929
Size: 7330
MluI A'CGCG_T
Cuts at: 7206 7206
Size: 7330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6444 6628 1305
Size: 5139 184 2007
Fragments arranged by size:
5139 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4275 10
Size: 54 1425 343 2443 3065
Fragments arranged by size:
3065 2443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2861 2994 3093 4281 5288 5647
Size: 648 1104 591 133 99 1188 1007 359
Cuts at: 5647 5806 518
Size: 159 2042
Fragments arranged by size:
2042 1188 1104 1007 648 591 359 159
133 99
MspA1I CmG'CkG
Cuts at: 1606 2163 3079 3760 4746 5423 6571 6816 1606
Size: 557 916 681 986 677 1148 245 2120
Fragments arranged by size:
2120 1148 986 916 681 677 557 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3121 3451 3610 513
Size: 552 939 1117 330 159 4233
Fragments arranged by size:
4233 1117 939 552 330 159
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7330
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7330
NotI GC'GGCC_GC
Cuts at: 4777 4777
Size: 7330
NsiI A_TGCA'T
Cuts at: 2188 2188
Size: 7330
NspI r_CATG'y
Cuts at: 1132 1229 2229 2574 2866 2993 3199 4214 4688
Size: 97 1000 345 292 127 206 1015 474
Cuts at: 4688 6233 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1015 1000 474 345 292 206
127 97
PasI CC'CwG_GG
Cuts at: 1348 4397 1348
Size: 3049 4281
PciI A'CATG_T
Cuts at: 2570 3195 6229 2570
Size: 625 3034 3671
Fragments arranged by size:
3671 3034 625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3127 3602 1108
Size: 2019 475 4836
Fragments arranged by size:
4836 2019 475
PmeI GTTT'AAAC
Cuts at: 4634 4634
Size: 7330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5399 5431 6912 6944 5399
Size: 32 1481 32 5785
Fragments arranged by size:
5785 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3870 1613
Size: 2257 5073
PsiI TTA'TAA
Cuts at: 3459 4205 4263 4900 3459
Size: 746 58 637 5889
Fragments arranged by size:
5889 746 637 58
PstI C_TGCA'G
Cuts at: 830 2168 3855 4682 830
Size: 1338 1687 827 3478
Fragments arranged by size:
3478 1687 1338 827
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7330
PvuII CAG'CTG
Cuts at: 2163 3760 2163
Size: 1597 5733
SacI G_AGCT'C
Cuts at: 3229 3229
Size: 7330
SalI G'TCGA_C
Cuts at: 1936 4672 1936
Size: 2736 4594
SbfI CC_TGCA'GG
Cuts at: 4682 4682
Size: 7330
ScaI AGT'ACT
Cuts at: 3754 5578 7290 3754
Size: 1824 1712 3794
Fragments arranged by size:
3794 1824 1712
SfcI C'TryA_G
Cuts at: 826 1045 2164 3851 4678 5813 6494 6685 826
Size: 219 1119 1687 827 1135 681 191 1471
Fragments arranged by size:
1687 1471 1135 1119 827 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7330
SmaI CCC'GGG
Cuts at: 1872 4517 4657 1872
Size: 2645 140 4545
Fragments arranged by size:
4545 2645 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 5434 6335 6597 820
Size: 197 926 114 3377 901 262 1553
Fragments arranged by size:
3377 1553 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 5121 493
Size: 4628 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7330
SphI G_CATG'C
Cuts at: 1132 4688 1132
Size: 3556 3774
SspI AAT'ATT
Cuts at: 5 52 2522 3547 4254 5254 5
Size: 47 2470 1025 707 1000 2081
Fragments arranged by size:
2470 2081 1025 1000 707 47
StuI AGG'CCT
Cuts at: 1517 2124 3069 3841 1517
Size: 607 945 772 5006
Fragments arranged by size:
5006 945 772 607
StyI C'CwwG_G
Cuts at: 513 1065 2004 2404 3121 3451 3610 3842 4769
Size: 552 939 400 717 330 159 232 927
Cuts at: 4769 513
Size: 3074
Fragments arranged by size:
3074 939 927 717 552 400 330 232
159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2250 3806 4085 4389 5357 5374 5527
Size: 1255 670 1556 279 304 968 17 153
Cuts at: 5527 5712 6157 325
Size: 185 445 1498
Fragments arranged by size:
1556 1498 1255 968 670 445 304 279
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5576 7288
Size: 274 80 33 51 1443 1775 1824 1712
Cuts at: 7288 96
Size: 138
Fragments arranged by size:
1824 1775 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2814 2846 3386 3418 4221 4253 4309 4341 7290
Size: 32 540 32 803 32 56 32 2949
Cuts at: 7290 7322 2814
Size: 32 2822
Fragments arranged by size:
2949 2822 803 540 56 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7330
XbaI T'CTAG_A
Cuts at: 4666 4666
Size: 7330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4479 4479
Size: 7330
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7330
XmaI C'CCGG_G
Cuts at: 1870 4515 4655 1870
Size: 2645 140 4545
Fragments arranged by size:
4545 2645 140
XmnI GAAnn'nnTTC
Cuts at: 2581 5459 2581
Size: 2878 4452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5138 276
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI