Restriction map for
pFN21AB7274
(Circular) (Six-base) MAPSORT of: pFN21AB7274.seq Check: 9738 from: 1 to: 6940
pFN21AB7274 6940 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4750 278
Size: 53 83 186 936 3214 2468
Fragments arranged by size:
3214 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4283 1937
Size: 2346 4594
Acc65I G'GTAC_C
Cuts at: 2931 4261 2931
Size: 1330 5610
AclI AA'CG_TT
Cuts at: 1563 5067 5440 1563
Size: 3504 373 3063
Fragments arranged by size:
3504 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 2647 2895 3494 3747 3873 3891 5003
Size: 930 85 248 599 253 126 18 1112
Cuts at: 5003 6387 6655 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1112 930 599 268 253 248
126 85 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6743
AflIII A'CryG_T
Cuts at: 1203 2371 3860 5839 6816 1203
Size: 1168 1489 1979 977 1327
Fragments arranged by size:
1979 1489 1327 1168 977
AgeI A'CCGG_T
Cuts at: 5771 5771
Size: 6940
AhdI GACnn_n'nnGTC
Cuts at: 5669 5669
Size: 6940
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2788 3623 1184
Size: 1604 835 4501
Fragments arranged by size:
4501 1604 835
AlwNI CAG_nnn'CTG
Cuts at: 3704 4683 6255 3704
Size: 979 1572 4389
Fragments arranged by size:
4389 1572 979
ApaLI G'TGCA_C
Cuts at: 3144 4997 6153 3144
Size: 1853 1156 3931
Fragments arranged by size:
3931 1853 1156
ApoI r'AATT_y
Cuts at: 1493 2292 2578 2631 2816 3054 3230 3299 3748
Size: 799 286 53 185 238 176 69 449
Cuts at: 3748 4213 4249 4475 4627 6736 1493
Size: 465 36 226 152 2109 1697
Fragments arranged by size:
2109 1697 799 465 449 286 238 226
185 176 152 69 53 36
AseI AT'TA_AT
Cuts at: 160 1032 5494 160
Size: 872 4462 1606
Fragments arranged by size:
4462 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
AvaI C'yCGr_G
Cuts at: 1870 1943 4265 1870
Size: 73 2322 4545
Fragments arranged by size:
4545 2322 73
BamHI G'GATC_C
Cuts at: 4270 4637 4270
Size: 367 6573
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2931 4261 5716 618
Size: 325 217 627 1144 1330 1455 1842
Fragments arranged by size:
1842 1455 1330 1144 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4259 1391
Size: 2868 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3638 928
Size: 971 1739 4230
Fragments arranged by size:
4230 1739 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5131 5165 1903
Size: 34 50 34 3110 34 3678
Fragments arranged by size:
3678 3110 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2223 4832 6042 2223
Size: 2609 1210 3121
Fragments arranged by size:
3121 2609 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6940
BfrBI ATG'CAT
Cuts at: 3463 3463
Size: 6940
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5551 136
Size: 107 122 71 631 605 3879 1525
Fragments arranged by size:
3879 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3884 6935 3884
Size: 3051 3889
BlpI GC'TnA_GC
Cuts at: 2086 4357 2086
Size: 2271 4669
Bme1580I G_kGCm'C
Cuts at: 1790 3148 5001 6157 1790
Size: 1358 1853 1156 2573
Fragments arranged by size:
2573 1853 1358 1156
BmgBI CAC'GTC
Cuts at: 1318 3863 1318
Size: 2545 4395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 3253 4671
Size: 416 53 83 399 24 1499 337 1418
Cuts at: 4671 5629 442
Size: 958 1753
Fragments arranged by size:
1753 1499 1418 958 416 399 337 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3669 3826 5600 1239
Size: 2430 157 1774 2579
Fragments arranged by size:
2579 2430 1774 157
Bpu10I CC'TnA_GC
Cuts at: 3763 3763
Size: 6940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5065 5930 6228 5065
Size: 865 298 5777
Fragments arranged by size:
5777 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3844 5603 882
Size: 617 2345 1759 2219
Fragments arranged by size:
2345 2219 1759 617
BsaAI yAC'GTr
Cuts at: 493 1204 2982 4731 493
Size: 711 1778 1749 2702
Fragments arranged by size:
2702 1778 1749 711
BsaBI GATnn'nnATC
Cuts at: 2550 6934 2550
Size: 4384 2556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4747 5129 5764 275
Size: 53 83 186 936 3214 382 635 1451
Fragments arranged by size:
3214 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5373 5771 6045 6192 6603 5373
Size: 398 274 147 411 5710
Fragments arranged by size:
5710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6715
Fragments arranged by size:
6715 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3035 1186
Size: 1849 5091
BseYI C'CCAG_C
Cuts at: 6143 6639 6143
Size: 496 6444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6367
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4390 5151 6179 1540
Size: 275 186 2389 761 1028 2301
Fragments arranged by size:
2389 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3148 5001 5086 6157 3148
Size: 1853 85 1071 3931
Fragments arranged by size:
3931 1853 1071 85
BsmI GAATG_Cn'
Cuts at: 2459 2885 3526 3786 4451 4544 2459
Size: 426 641 260 665 93 4855
Fragments arranged by size:
4855 665 641 426 260 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3148 4259 5001 5086 6157 1391
Size: 399 1358 1111 742 85 1071 2174
Fragments arranged by size:
2174 1358 1111 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6603 6603
Size: 6940
BspHI T'CATG_A
Cuts at: 4829 6559 4829
Size: 1730 5210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4295 844
Size: 3451 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4827 1394
Size: 429 3004 3507
Fragments arranged by size:
3507 3004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3465 5435 5609 65
Size: 1183 2217 1970 174 1396
Fragments arranged by size:
2217 1970 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5584 5771 1700
Size: 110 3774 187 2869
Fragments arranged by size:
3774 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2809 96
Size: 2713 4227
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6940
BssSI C'ACGA_G
Cuts at: 5000 6012 5000
Size: 1012 5928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6940
BstBI TT'CG_AA
Cuts at: 5744 5744
Size: 6940
BstEII G'GTnAC_C
Cuts at: 1184 2620 1184
Size: 1436 5504
BstYI r'GATC_y
Cuts at: 1972 2306 2551 3884 4270 4637 5022 5039 6491
Size: 334 245 1333 386 367 385 17 1452
Cuts at: 6491 6577 6589 6935 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1333 386 385 367 346 334
245 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6201
Fragments arranged by size:
6201 683 56
BtsI GCAGTG_nn'
Cuts at: 2525 4456 5250 5270 2525
Size: 1931 794 20 4195
Fragments arranged by size:
4195 1931 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6905
DraI TTT'AAA
Cuts at: 3334 4244 3334
Size: 910 6030
DraIII CAC_nnn'GTG
Cuts at: 3312 3312
Size: 6940
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5947 809
Size: 507 4631 1802
Fragments arranged by size:
4631 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2846 4387 5276
Size: 54 1425 50 275 18 1016 1541 889
Cuts at: 5276 6905 8
Size: 1629 43
Fragments arranged by size:
1629 1541 1425 1016 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4387 1537
Size: 275 2575 4090
Fragments arranged by size:
4090 2575 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2163 2448 3972 3999 4870 1353
Size: 804 6 285 1524 27 871 3423
Fragments arranged by size:
3423 1524 871 804 285 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5527 5901 6047 387
Size: 1064 138 3938 374 146 1280
Fragments arranged by size:
3938 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 2647 2895 3494 3669 3747 3826
Size: 393 930 85 248 599 175 78 79
Cuts at: 3826 3873 3891 5003 5600 6387 6655 1239
Size: 47 18 1112 597 787 268 1524
Fragments arranged by size:
1524 1112 930 787 599 597 393 268
248 175 85 79 78 47 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3942 3942
Size: 6940
EcoO109I rG'GnC_Cy
Cuts at: 2905 2905
Size: 6940
EcoRI G'AATT_C
Cuts at: 4213 4249 4627 4213
Size: 36 378 6526
Fragments arranged by size:
6526 378 36
EcoRV GAT'ATC
Cuts at: 4174 4174
Size: 6940
FspI TGC'GCA
Cuts at: 5446 5446
Size: 6940
HaeII r_GCGC'y
Cuts at: 1678 6087 1678
Size: 4409 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3371 3403 4219 4251 5581
Size: 32 135 32 1799 32 816 32 1330
Cuts at: 5581 5613 5655 5687 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1799 1330 816 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 4284 4530 669
Size: 988 281 732 1614 246 3079
Fragments arranged by size:
3079 1614 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 3214 748
Size: 2466 4474
HpaI GTT'AAC
Cuts at: 2670 4530 2670
Size: 1860 5080
KpnI G_GTAC'C
Cuts at: 2935 4265 2935
Size: 1330 5610
MfeI C'AATT_G
Cuts at: 4539 4539
Size: 6940
MluI A'CGCG_T
Cuts at: 6816 6816
Size: 6940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 3190 6054 6238 1305
Size: 958 927 2864 184 2007
Fragments arranged by size:
2864 2007 958 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2848 10
Size: 54 1425 343 1016 4102
Fragments arranged by size:
4102 1425 1016 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3605 4898 5257 5416 518
Size: 648 2439 1293 359 159 2042
Fragments arranged by size:
2439 2042 1293 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3689 4124 4356 5033 6181 6426 1606
Size: 2083 435 232 677 1148 245 2120
Fragments arranged by size:
2120 2083 1148 677 435 245 232
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6388
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6940
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6940
NotI GC'GGCC_GC
Cuts at: 4387 4387
Size: 6940
NsiI A_TGCA'T
Cuts at: 3465 3465
Size: 6940
NspI r_CATG'y
Cuts at: 1132 1229 2375 3467 3604 4298 5843 1132
Size: 97 1146 1092 137 694 1545 2229
Fragments arranged by size:
2229 1545 1146 1092 694 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6940
PciI A'CATG_T
Cuts at: 2371 5839 2371
Size: 3468 3472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6940
PmeI GTTT'AAAC
Cuts at: 4244 4244
Size: 6940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5009 5041 6522 6554 5009
Size: 32 1481 32 5395
Fragments arranged by size:
5395 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2905 2905
Size: 6940
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6940
PsiI TTA'TAA
Cuts at: 4510 4510
Size: 6940
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 6908
PstI C_TGCA'G
Cuts at: 830 2181 2532 4292 830
Size: 1351 351 1760 3478
Fragments arranged by size:
3478 1760 1351 351
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6940
PvuII CAG'CTG
Cuts at: 3689 3689
Size: 6940
SalI G'TCGA_C
Cuts at: 1936 4282 1936
Size: 2346 4594
SanDI GG'GwC_CC
Cuts at: 2905 2905
Size: 6940
SbfI CC_TGCA'GG
Cuts at: 4292 4292
Size: 6940
ScaI AGT'ACT
Cuts at: 5188 6900 5188
Size: 1712 5228
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 6940
SfcI C'TryA_G
Cuts at: 826 1045 2177 2528 3939 4288 5423 6104 6295
Size: 219 1132 351 1411 349 1135 681 191
Cuts at: 6295 826
Size: 1471
Fragments arranged by size:
1471 1411 1135 1132 681 351 349 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6940
SmaI CCC'GGG
Cuts at: 1872 4267 1872
Size: 2395 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5044 5945 6207 820
Size: 197 926 3101 901 262 1553
Fragments arranged by size:
3101 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4731 493
Size: 4238 2702
SpeI A'CTAG_T
Cuts at: 152 3620 152
Size: 3468 3472
SphI G_CATG'C
Cuts at: 1132 4298 1132
Size: 3166 3774
SspI AAT'ATT
Cuts at: 5 52 4864 5
Size: 47 4812 2081
Fragments arranged by size:
4812 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6940
StyI C'CwwG_G
Cuts at: 513 1065 2097 2909 4379 513
Size: 552 1032 812 1470 3074
Fragments arranged by size:
3074 1470 1032 812 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3163 3289 3304 4967 4984 5137 5322
Size: 1255 1583 126 15 1663 17 153 185
Cuts at: 5322 5767 325
Size: 445 1498
Fragments arranged by size:
1663 1583 1498 1255 445 185 153 126
17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2809 3114 3130
Size: 274 80 33 51 1443 832 305 16
Cuts at: 3130 4094 4157 5186 6898 96
Size: 964 63 1029 1712 138
Fragments arranged by size:
1712 1443 1029 964 832 305 274 138
80 63 51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6900 6932 6900
Size: 32 6908
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6940
XbaI T'CTAG_A
Cuts at: 4276 4276
Size: 6940
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6940
XmaI C'CCGG_G
Cuts at: 1870 4265 1870
Size: 2395 4545
XmnI GAAnn'nnTTC
Cuts at: 3300 3786 4113 5069 3300
Size: 486 327 956 5171
Fragments arranged by size:
5171 956 486 327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4748 276
Size: 53 83 186 936 3214 2468
Fragments arranged by size:
3214 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI XcmI