Restriction map for
pFN21AB7259
(Circular) (Six-base) MAPSORT of: pFN21AB7259.seq Check: 929 from: 1 to: 6463
pFN21AB7259 6463 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 6463
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2599 3678 3806 1937
Size: 662 1079 128 4594
Fragments arranged by size:
4594 1079 662 128
Acc65I G'GTAC_C
Cuts at: 3784 3784
Size: 6463
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3144 4526 5910 6178 1632
Size: 1512 1382 1384 268 1917
Fragments arranged by size:
1917 1512 1384 1382 268
AfeI AGC'GCT
Cuts at: 2072 2090 2072
Size: 18 6445
AflII C'TTAA_G
Cuts at: 820 1017 2842 3091 820
Size: 197 1825 249 4192
Fragments arranged by size:
4192 1825 249 197
AflIII A'CryG_T
Cuts at: 1203 5362 6339 1203
Size: 4159 977 1327
Fragments arranged by size:
4159 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 2812 2911 5192 2812
Size: 99 2281 4083
Fragments arranged by size:
4083 2281 99
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 2144
Size: 32 6431
AlwNI CAG_nnn'CTG
Cuts at: 4206 5778 4206
Size: 1572 4891
ApaI G_GGCC'C
Cuts at: 2222 2222
Size: 6463
ApaLI G'TGCA_C
Cuts at: 2026 2330 4520 5676 2026
Size: 304 2190 1156 2813
Fragments arranged by size:
2813 2190 1156 304
ApoI r'AATT_y
Cuts at: 1493 2240 3121 3587 3772 3998 4150 6259 1493
Size: 747 881 466 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 747 466 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 3468 5017 160
Size: 872 2436 1549 1606
Fragments arranged by size:
2436 1606 1549 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2752 3788 1870
Size: 73 111 698 1036 4545
Fragments arranged by size:
4545 1036 698 111 73
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2743 2964 3206 3566 3784
Size: 325 217 627 956 221 242 360 218
Cuts at: 3784 5239 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 956 627 360 325 242 221
218 217
BanII G_rGCy'C
Cuts at: 1391 2222 2737 3197 3782 1391
Size: 831 515 460 585 4072
Fragments arranged by size:
4072 831 585 515 460
BbeI G_GCGC'C
Cuts at: 2968 2968
Size: 6463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2587 2808 3180 3513 3710 928
Size: 971 688 221 372 333 197 3681
Fragments arranged by size:
3681 971 688 372 333 221 197
BbvCI CC'TCA_GC
Cuts at: 2189 2189
Size: 6463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4654 4688 1903
Size: 34 2717 34 3678
Fragments arranged by size:
3678 2717 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4355 5565 4355
Size: 1210 5253
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6463
BfrBI ATG'CAT
Cuts at: 3398 3398
Size: 6463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5074 136
Size: 107 122 71 631 605 3402 1525
Fragments arranged by size:
3402 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3325 6458 3325
Size: 3133 3330
BlpI GC'TnA_GC
Cuts at: 3880 3880
Size: 6463
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2222 2334 3189 3571 4524 5680 1790
Size: 240 192 112 855 382 953 1156 2573
Fragments arranged by size:
2573 1156 953 855 382 240 192 112
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3308 4194 5152
Size: 416 53 83 399 24 1891 886 958
Cuts at: 5152 442
Size: 1753
Fragments arranged by size:
1891 1753 958 886 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 2387 2680 2797 3435 3701 5123 1239
Size: 907 241 293 117 638 266 1422 2579
Fragments arranged by size:
2579 1422 907 638 293 266 241 117
Bpu10I CC'TnA_GC
Cuts at: 2189 2736 2940 3285 3557 2189
Size: 547 204 345 272 5095
Fragments arranged by size:
5095 547 345 272 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3256 3314 4588 5453 5751 3256
Size: 58 1274 865 298 3968
Fragments arranged by size:
3968 1274 865 298 58
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5126 882
Size: 617 3627 2219
Fragments arranged by size:
3627 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 4254 493
Size: 711 2372 678 2702
Fragments arranged by size:
2702 2372 711 678
BsaBI GATnn'nnATC
Cuts at: 2868 6457 2868
Size: 3589 2874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2965 4270 4652 5287
Size: 53 83 186 936 1432 1305 382 635
Cuts at: 5287 275
Size: 1451
Fragments arranged by size:
1451 1432 1305 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4896 5294 5568 5715 6126 4896
Size: 398 274 147 411 5233
Fragments arranged by size:
5233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2393 2423 2911
Size: 30 165 30 741 30 219 30 488
Cuts at: 2911 2941 3699 3729 1178
Size: 30 758 30 3912
Fragments arranged by size:
3912 758 741 488 219 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2401 2455 2987 3348 3548 1186
Size: 1215 54 532 361 200 4101
Fragments arranged by size:
4101 1215 532 361 200 54
BseYI C'CCAG_C
Cuts at: 2222 5666 6162 2222
Size: 3444 496 2523
Fragments arranged by size:
3444 2523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2220 2397 2570 1163
Size: 573 484 177 173 5056
Fragments arranged by size:
5056 573 484 177 173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2334 2737 3540 3725 4524 4609 5680 2030
Size: 304 403 803 185 799 85 1071 2813
Fragments arranged by size:
2813 1071 803 799 403 304 185 85
BsmI GAATG_Cn'
Cuts at: 3014 3974 4067 3014
Size: 960 93 5410
Fragments arranged by size:
5410 960 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2222 2334 2737 3189 3197 3540
Size: 399 240 192 112 403 452 8 343
Cuts at: 3540 3571 3725 3782 4524 4609 5680 1391
Size: 31 154 57 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 403 399 343 240
192 154 112 85 57 31 8
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 4352 6082 4352
Size: 1730 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 3123 3818 844
Size: 1467 812 695 3489
Fragments arranged by size:
3489 1467 812 695
BsrBI CCG'CTC
Cuts at: 1394 1823 2121 2689 4350 1394
Size: 429 298 568 1661 3507
Fragments arranged by size:
3507 1661 568 429 298
BsrDI GCAATG_nn'
Cuts at: 65 1248 2825 4958 5132 65
Size: 1183 1577 2133 174 1396
Fragments arranged by size:
2133 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 5107 5294 1700
Size: 110 220 3077 187 2869
Fragments arranged by size:
3077 2869 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2317 794
Size: 1523 4940
BstBI TT'CG_AA
Cuts at: 5267 5267
Size: 6463
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 2729 2704
Size: 25 6438
BstYI r'GATC_y
Cuts at: 1972 3325 3793 4160 4545 4562 6014 6100 6112
Size: 1353 468 367 385 17 1452 86 12
Cuts at: 6112 6458 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1353 468 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2760 2760
Size: 6463
BtgI C'CryG_G
Cuts at: 513 1065 2385 2722 513
Size: 552 1320 337 4254
Fragments arranged by size:
4254 1320 552 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 2028 2208 2734 3226 3568 3979 4773 4793 2028
Size: 180 526 492 342 411 794 20 3698
Fragments arranged by size:
3698 794 526 492 411 342 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2957 2992 529
Size: 35 2393 35 4000
Fragments arranged by size:
4000 2393 35 35
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DraIII CAC_nnn'GTG
Cuts at: 2990 2990
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 2359 3350
Size: 54 1425 50 275 18 468 61 991
Cuts at: 3350 3910 4799 6428 8
Size: 560 889 1629 43
Fragments arranged by size:
1629 1425 991 889 560 468 275 61
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 4393 1353
Size: 1190 1850 3423
Fragments arranged by size:
3423 1850 1190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5050 5424 5570 387
Size: 1064 138 3461 374 146 1280
Fragments arranged by size:
3461 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2735 2735
Size: 6463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2387 2680 2797 3144 3435 3701
Size: 393 514 241 293 117 347 291 266
Cuts at: 3701 4526 5123 5910 6178 1239
Size: 825 597 787 268 1524
Fragments arranged by size:
1524 825 787 597 514 393 347 293
291 268 266 241 117
EcoO109I rG'GnC_Cy
Cuts at: 2219 3411 2219
Size: 1192 5271
EcoRI G'AATT_C
Cuts at: 3772 4150 3772
Size: 378 6085
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 2074 2092 2968 5610 1678
Size: 396 18 876 2642 2531
Fragments arranged by size:
2642 2531 876 396 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2606 2638 2732 2764 2798
Size: 32 135 32 1034 32 94 32 34
Cuts at: 2798 2830 2897 2929 3697 3729 5104 5136 5178
Size: 32 67 32 768 32 1375 32 42
Cuts at: 5178 5210 1373
Size: 32 2626
Fragments arranged by size:
2626 1375 1034 768 135 94 67 42
34 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 3807 4053 669
Size: 988 281 736 1133 246 3079
Fragments arranged by size:
3079 1133 988 736 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KasI G'GCGC_C
Cuts at: 2964 2964
Size: 6463
KpnI G_GTAC'C
Cuts at: 3788 3788
Size: 6463
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2589 2940 5577 5761 1305
Size: 1284 351 2637 184 2007
Fragments arranged by size:
2637 2007 1284 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 2361 10
Size: 54 1425 343 468 61 4112
Fragments arranged by size:
4112 1425 468 343 61 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2727 3251 3527 4421 4780 4939
Size: 648 1011 550 524 276 894 359 159
Cuts at: 4939 518
Size: 2042
Fragments arranged by size:
2042 1011 894 648 550 524 359 276
159
MspA1I CmG'CkG
Cuts at: 1606 3879 4556 5704 5949 1606
Size: 2273 677 1148 245 2120
Fragments arranged by size:
2273 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2965 2965
Size: 6463
NcoI C'CATG_G
Cuts at: 513 1065 2385 2722 513
Size: 552 1320 337 4254
Fragments arranged by size:
4254 1320 552 337
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NsiI A_TGCA'T
Cuts at: 3400 3400
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2216 3398 3821 5366 1132
Size: 97 987 1182 423 1545 2229
Fragments arranged by size:
2229 1545 1182 987 423 97
PasI CC'CwG_GG
Cuts at: 1348 2802 1348
Size: 1454 5009
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6463
PfoI T'CCnGG_A
Cuts at: 3387 3387
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2176 4532 4564 6045 6077 2144
Size: 32 2356 32 1481 32 2530
Fragments arranged by size:
2530 2356 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3411 3411
Size: 6463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 4033 4033
Size: 6463
PspOMI G'GGCC_C
Cuts at: 2218 2218
Size: 6463
PspXI vC'TCGA_Gb
Cuts at: 2752 2752
Size: 6463
PstI C_TGCA'G
Cuts at: 830 2421 2685 3233 3815 830
Size: 1591 264 548 582 3478
Fragments arranged by size:
3478 1591 582 548 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
SacI G_AGCT'C
Cuts at: 2737 2737
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 2144 4711 6423 2144
Size: 2567 1712 2184
Fragments arranged by size:
2567 2184 1712
SexAI A'CCwGG_T
Cuts at: 2292 2292
Size: 6463
SfcI C'TryA_G
Cuts at: 826 1045 2066 2146 2417 2681 3229 3811 4946
Size: 219 1021 80 271 264 548 582 1135
Cuts at: 4946 5627 5818 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1021 681 582 548 271 264
219 191 80
SfoI GGC'GCC
Cuts at: 2966 2966
Size: 6463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 2842 3091 3271 3329 4567
Size: 197 926 809 90 249 180 58 1238
Cuts at: 4567 5468 5730 820
Size: 901 262 1553
Fragments arranged by size:
1553 1238 926 901 809 262 249 197
180 90 58
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 3821 1132
Size: 2689 3774
SspI AAT'ATT
Cuts at: 5 52 4387 5
Size: 47 4335 2081
Fragments arranged by size:
4335 2081 47
StuI AGG'CCT
Cuts at: 1517 2758 1517
Size: 1241 5222
StyI C'CwwG_G
Cuts at: 513 1065 2356 2385 2722 3902 513
Size: 552 1291 29 337 1180 3074
Fragments arranged by size:
3074 1291 1180 552 337 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2815 4490 4507 4660 4845 5290
Size: 1255 737 498 1675 17 153 185 445
Cuts at: 5290 325
Size: 1498
Fragments arranged by size:
1675 1498 1255 737 498 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2981 4709
Size: 274 80 33 51 1443 165 839 1728
Cuts at: 4709 6421 96
Size: 1712 138
Fragments arranged by size:
1728 1712 1443 839 274 165 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2911 2943 3004 3036 3699 3731 6423 6455 2911
Size: 32 61 32 663 32 2692 32 2919
Fragments arranged by size:
2919 2692 663 61 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3683 1316
Size: 2367 4096
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2369
Size: 6463
XhoI C'TCGA_G
Cuts at: 1943 2752 1943
Size: 809 5654
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 2252 4592 2252
Size: 2340 4123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BsmBI BstZ17I EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI
SwaI