Restriction map for
pFN21AB7254
(Circular) (Six-base) MAPSORT of: pFN21AB7254.seq Check: 5776 from: 1 to: 6637
pFN21AB7254 6637 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2200 2200
Size: 6637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3534 4447 278
Size: 53 83 186 936 1998 913 2468
Fragments arranged by size:
2468 1998 936 913 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2940 3980 1937
Size: 1003 1040 4594
Fragments arranged by size:
4594 1040 1003
Acc65I G'GTAC_C
Cuts at: 3958 3958
Size: 6637
AclI AA'CG_TT
Cuts at: 1563 4764 5137 1563
Size: 3201 373 3063
Fragments arranged by size:
3201 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4700 6084 6352 1632
Size: 3068 1384 268 1917
Fragments arranged by size:
3068 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2897 820
Size: 197 1880 4560
Fragments arranged by size:
4560 1880 197
AflIII A'CryG_T
Cuts at: 1203 2361 3651 3897 5536 6513 1203
Size: 1158 1290 246 1639 977 1327
Fragments arranged by size:
1639 1327 1290 1158 977 246
AgeI A'CCGG_T
Cuts at: 5468 5468
Size: 6637
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 2439 2552 3908 1166
Size: 1273 113 1356 3895
Fragments arranged by size:
3895 1356 1273 113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 6605
AlwNI CAG_nnn'CTG
Cuts at: 2874 3700 4380 5952 2874
Size: 826 680 1572 3559
Fragments arranged by size:
3559 1572 826 680
ApaI G_GGCC'C
Cuts at: 3165 3165
Size: 6637
ApaLI G'TGCA_C
Cuts at: 4694 5850 4694
Size: 1156 5481
ApoI r'AATT_y
Cuts at: 1493 2285 2908 3946 4172 4324 6433 1493
Size: 792 623 1038 226 152 2109 1697
Fragments arranged by size:
2109 1697 1038 792 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5191 160
Size: 872 4159 1606
Fragments arranged by size:
4159 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 3328 3962 1870
Size: 73 1385 634 4545
Fragments arranged by size:
4545 1385 634 73
BamHI G'GATC_C
Cuts at: 3716 3967 4334 3716
Size: 251 367 6019
Fragments arranged by size:
6019 367 251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 2637 2816 3843 3958
Size: 325 217 627 759 91 179 1027 115
Cuts at: 3958 5413 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1027 759 627 325 217 179
115 91
BanII G_rGCy'C
Cuts at: 1391 2561 2604 3165 3691 3956 1391
Size: 1170 43 561 526 265 4072
Fragments arranged by size:
4072 1170 561 526 265 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 2326 2509 928
Size: 971 282 145 183 5056
Fragments arranged by size:
5056 971 282 183 145
BbvCI CC'TCA_GC
Cuts at: 2645 3219 2645
Size: 574 6063
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4828 4862 1903
Size: 34 50 34 2807 34 3678
Fragments arranged by size:
3678 2807 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4529 5739 4529
Size: 1210 5427
BclI T'GATC_A
Cuts at: 1548 2931 3057 1548
Size: 1383 126 5128
Fragments arranged by size:
5128 1383 126
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 5248 136
Size: 107 122 71 631 605 430 3146 1525
Fragments arranged by size:
3146 1525 631 605 430 122 107 71
BglII A'GATC_T
Cuts at: 2418 6632 2418
Size: 4214 2423
BlpI GC'TnA_GC
Cuts at: 2075 2560 4054 2075
Size: 485 1494 4658
Fragments arranged by size:
4658 1494 485
Bme1580I G_kGCm'C
Cuts at: 1790 3165 3175 4698 5854 1790
Size: 1375 10 1523 1156 2573
Fragments arranged by size:
2573 1523 1375 1156 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4368 5326 442
Size: 416 53 83 399 24 2951 958 1753
Fragments arranged by size:
2951 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5297 1239
Size: 4058 2579
Bpu10I CC'TnA_GC
Cuts at: 2645 3219 2645
Size: 574 6063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4762 5627 5925 4762
Size: 865 298 5474
Fragments arranged by size:
5474 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3683 5300 882
Size: 617 2184 1617 2219
Fragments arranged by size:
2219 2184 1617 617
BsaAI yAC'GTr
Cuts at: 493 1204 4428 493
Size: 711 3224 2702
Fragments arranged by size:
3224 2702 711
BsaBI GATnn'nnATC
Cuts at: 6631 6631
Size: 6637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3531 3798 4444 4826
Size: 53 83 186 936 1998 267 646 382
Cuts at: 4826 5461 275
Size: 635 1451
Fragments arranged by size:
1998 1451 936 646 635 382 267 186
83 53
BsaWI w'CCGG_w
Cuts at: 2247 5070 5468 5742 5889 6300 2247
Size: 2823 398 274 147 411 2584
Fragments arranged by size:
2823 2584 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3413 3443 1178
Size: 30 165 30 2010 30 4372
Fragments arranged by size:
4372 2010 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2818 3284 3293 3839 1186
Size: 1632 466 9 546 3984
Fragments arranged by size:
3984 1632 546 466 9
BseYI C'CCAG_C
Cuts at: 2453 2609 3164 3417 3560 3832 5840 6336 2453
Size: 156 555 253 143 272 2008 496 2754
Fragments arranged by size:
2754 2008 555 496 272 253 156 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2248 2417 1163
Size: 573 512 169 5383
Fragments arranged by size:
5383 573 512 169
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2012 4087 4848 5876 1540
Size: 275 186 11 2075 761 1028 2301
Fragments arranged by size:
2301 2075 1028 761 275 186 11
BsiHKAI G_wGCw'C
Cuts at: 2349 4698 4783 5854 2349
Size: 2349 85 1071 3132
Fragments arranged by size:
3132 2349 1071 85
BsmI GAATG_Cn'
Cuts at: 4148 4241 4148
Size: 93 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2561 2604 3165 3175 3691 3956
Size: 399 559 212 43 561 10 516 265
Cuts at: 3956 4698 4783 5854 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 559 516 399 265
212 85 43 10
BspEI T'CCGG_A
Cuts at: 2247 6300 2247
Size: 4053 2584
BspHI T'CATG_A
Cuts at: 2766 3000 3882 4526 6256 2766
Size: 234 882 644 1730 3147
Fragments arranged by size:
3147 1730 882 644 234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2200 2605 3924 3992 844
Size: 1356 405 1319 68 3489
Fragments arranged by size:
3489 1356 1319 405 68
BsrBI CCG'CTC
Cuts at: 1394 1823 4524 1394
Size: 429 2701 3507
Fragments arranged by size:
3507 2701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5281 5468 1700
Size: 110 3471 187 2869
Fragments arranged by size:
3471 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6637
BssHII G'CGCG_C
Cuts at: 1925 2031 1925
Size: 106 6531
BssSI C'ACGA_G
Cuts at: 4697 5709 4697
Size: 1012 5625
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2262 2874 794
Size: 1468 612 4557
Fragments arranged by size:
4557 1468 612
BstBI TT'CG_AA
Cuts at: 2995 5441 2995
Size: 2446 4191
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6637
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2467
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 2418 3716 3967 4334 4719 4736 6188 6274
Size: 446 1298 251 367 385 17 1452 86
Cuts at: 6274 6286 6632 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1298 446 385 367 346 251
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3039 3144 513
Size: 552 1974 105 4006
Fragments arranged by size:
4006 1974 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2547 487
Size: 683 56 1321 4577
Fragments arranged by size:
4577 1321 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 4153 4947 4967 3175
Size: 978 794 20 4845
Fragments arranged by size:
4845 978 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6602
DraI TTT'AAA
Cuts at: 3941 3941
Size: 6637
DraIII CAC_nnn'GTG
Cuts at: 3145 3459 3145
Size: 314 6323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2399 5644 809
Size: 507 1083 3245 1802
Fragments arranged by size:
3245 1802 1083 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2009 2376 2841
Size: 54 1425 50 275 18 179 367 465
Cuts at: 2841 3042 3117 3365 3585 3600 4084 4973 6602
Size: 201 75 248 220 15 484 889 1629
Cuts at: 6602 8
Size: 43
Fragments arranged by size:
1629 1425 889 484 465 367 275 248
220 201 179 75 54 50 43 18
15
EagI C'GGCC_G
Cuts at: 1537 1812 2009 4084 1537
Size: 275 197 2075 4090
Fragments arranged by size:
4090 2075 275 197
EarI CTCTTCn'nnn_
Cuts at: 1353 3270 4567 1353
Size: 1917 1297 3423
Fragments arranged by size:
3423 1917 1297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5224 5598 5744 387
Size: 1064 138 3635 374 146 1280
Fragments arranged by size:
3635 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4700 5297 6084 6352 1239
Size: 393 3068 597 787 268 1524
Fragments arranged by size:
3068 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2065 3161 2065
Size: 1096 5541
EcoRI G'AATT_C
Cuts at: 2285 3946 4324 2285
Size: 1661 378 4598
Fragments arranged by size:
4598 1661 378
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 2108 2431 5784 1678
Size: 430 323 3353 2531
Fragments arranged by size:
3353 2531 430 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2936 2968 3311 3343 3701
Size: 32 135 32 1364 32 343 32 358
Cuts at: 3701 3708 3733 3740 5278 5310 5352 5384 1373
Size: 7 25 7 1538 32 42 32 2626
Fragments arranged by size:
2626 1538 1364 358 343 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 3981 4227 669
Size: 988 281 895 1148 246 3079
Fragments arranged by size:
3079 1148 988 895 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6637
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KpnI G_GTAC'C
Cuts at: 3962 3962
Size: 6637
MfeI C'AATT_G
Cuts at: 4236 4236
Size: 6637
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3657 5751 5935 1305
Size: 2352 2094 184 2007
Fragments arranged by size:
2352 2094 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 2843 3044 3119 3587
Size: 54 1425 343 546 465 201 75 468
Cuts at: 3587 3602 10
Size: 15 3045
Fragments arranged by size:
3045 1425 546 468 465 343 201 75
54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2439 2465 2552 3485 3626 3908
Size: 648 1194 79 26 87 933 141 282
Cuts at: 3908 4595 4954 5113 518
Size: 687 359 159 2042
Fragments arranged by size:
2042 1194 933 687 648 359 282 159
141 87 79 26
MspA1I CmG'CkG
Cuts at: 1606 2474 2564 3370 3820 4053 4730 5878 6123
Size: 868 90 806 450 233 677 1148 245
Cuts at: 6123 1606
Size: 2120
Fragments arranged by size:
2120 1148 868 806 677 450 245 233
90
NcoI C'CATG_G
Cuts at: 513 1065 3039 513
Size: 552 1974 4111
Fragments arranged by size:
4111 1974 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 2365 3055 3995 5540 1132
Size: 97 1136 690 940 1545 2229
Fragments arranged by size:
2229 1545 1136 940 690 97
PasI CC'CwG_GG
Cuts at: 1348 3517 3684 1348
Size: 2169 167 4301
Fragments arranged by size:
4301 2169 167
PciI A'CATG_T
Cuts at: 2361 5536 2361
Size: 3175 3462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3459 1108
Size: 2351 4286
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4706 4738 6219 6251 3413
Size: 32 1261 32 1481 32 3799
Fragments arranged by size:
3799 1481 1261 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 6637
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6637
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PspOMI G'GGCC_C
Cuts at: 3161 3161
Size: 6637
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 6605
PstI C_TGCA'G
Cuts at: 830 3696 3989 830
Size: 2866 293 3478
Fragments arranged by size:
3478 2866 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 3820 3820
Size: 6637
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 4885 6597 4885
Size: 1712 4925
SfcI C'TryA_G
Cuts at: 826 1045 3692 3985 5120 5801 5992 826
Size: 219 2647 293 1135 681 191 1471
Fragments arranged by size:
2647 1471 1135 681 293 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 3964 1872
Size: 2092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 4741 5642 5904 820
Size: 197 926 954 1844 901 262 1553
Fragments arranged by size:
1844 1553 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 3995 1132
Size: 2863 3774
SspI AAT'ATT
Cuts at: 5 52 4561 5
Size: 47 4509 2081
Fragments arranged by size:
4509 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6637
StyI C'CwwG_G
Cuts at: 513 1065 2409 3039 4076 513
Size: 552 1344 630 1037 3074
Fragments arranged by size:
3074 1344 1037 630 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2494 3665 3831 4664 4681 4834 5019
Size: 1255 914 1171 166 833 17 153 185
Cuts at: 5019 5464 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1171 914 833 445 185 166
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4883 6595 96
Size: 274 80 33 51 1443 2906 1712 138
Fragments arranged by size:
2906 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2800 2832 2957 2989 6597 6629 2800
Size: 32 125 32 3608 32 2808
Fragments arranged by size:
3608 2808 125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 3595 3610 2533
Size: 1062 15 5560
Fragments arranged by size:
5560 1062 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6637
XmaI C'CCGG_G
Cuts at: 1870 3962 1870
Size: 2092 4545
XmnI GAAnn'nnTTC
Cuts at: 3428 4766 3428
Size: 1338 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3532 4445 276
Size: 53 83 186 936 1998 913 2468
Fragments arranged by size:
2468 1998 936 913 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI