Restriction map for
pFN21AB7253
(Circular) (Six-base) MAPSORT of: pFN21AB7253.seq Check: 1894 from: 1 to: 5962
pFN21AB7253 5962 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2901 4025 5409 5677 1632
Size: 1269 1124 1384 268 1917
Fragments arranged by size:
1917 1384 1269 1124 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2968 1184
Size: 1784 4178
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2551 2562 2635 2758 3036 3271 3497 3649
Size: 1058 11 73 123 278 235 226 152
Cuts at: 3649 5758 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1058 278 235 226 152 123
73 11
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3281 1391
Size: 1890 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 3003 928
Size: 971 441 663 3887
Fragments arranged by size:
3887 971 663 441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4153 4187 1903
Size: 34 50 34 2132 34 3678
Fragments arranged by size:
3678 2132 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3036 3854 5064 3036
Size: 818 1210 3934
Fragments arranged by size:
3934 1210 818
BclI T'GATC_A
Cuts at: 1548 3061 1548
Size: 1513 4449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3014 3693 4651
Size: 416 53 83 399 24 1597 679 958
Cuts at: 4651 442
Size: 1753
Fragments arranged by size:
1753 1597 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2583 1056
Size: 1527 4435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2007 4622 1239
Size: 768 2615 2579
Fragments arranged by size:
2615 2579 768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2715 4087 4952 5250 2715
Size: 1372 865 298 3427
Fragments arranged by size:
3427 1372 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5962
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2938 2961 1163
Size: 573 1202 23 4164
Fragments arranged by size:
4164 1202 573 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 2325 2581 3473 3566 2325
Size: 256 892 93 4721
Fragments arranged by size:
4721 892 256 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3281 4023 4108 5179 1391
Size: 399 1491 742 85 1071 2174
Fragments arranged by size:
2174 1491 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3317 844
Size: 2473 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 2092 3041 96
Size: 1996 949 3017
Fragments arranged by size:
3017 1996 949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2948 794
Size: 2154 3808
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 3111 3292 3659 4044 4061 5513 5599 5611
Size: 1139 181 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1139 385 367 346 181 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3252 3259 3478 4272 4292 3252
Size: 7 219 794 20 4922
Fragments arranged by size:
4922 794 219 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2763 2798 529
Size: 35 2199 35 3693
Fragments arranged by size:
3693 2199 35 35
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3203 4969 809
Size: 507 1887 1766 1802
Fragments arranged by size:
1887 1802 1766 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 3409 4298
Size: 54 1425 50 275 18 1171 408 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1171 889 408 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2988 3892 1353
Size: 1635 904 3423
Fragments arranged by size:
3423 1635 904
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2901 4025 4622 5409 5677 1239
Size: 393 375 894 1124 597 787 268 1524
Fragments arranged by size:
1524 1124 894 787 597 393 375 268
EcoO109I rG'GnC_Cy
Cuts at: 2141 2141
Size: 5962
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2364 2364
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 4603 4635 4677
Size: 32 135 32 1019 32 1980 32 42
Cuts at: 4677 4709 1373
Size: 32 2626
Fragments arranged by size:
2626 1980 1019 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2512 3306 3552 669
Size: 988 281 574 794 246 3079
Fragments arranged by size:
3079 988 794 574 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3003 10
Size: 54 1425 343 1171 2969
Fragments arranged by size:
2969 1425 1171 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 3920 4279 4438 518
Size: 648 889 1865 359 159 2042
Fragments arranged by size:
2042 1865 889 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3378 4055 5203 5448 1606
Size: 1772 677 1148 245 2120
Fragments arranged by size:
2120 1772 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 2579 1052
Size: 1527 4435
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2518 3320 4865 1132
Size: 97 1289 802 1545 2229
Fragments arranged by size:
2229 1545 1289 802 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 2276 2360 1108
Size: 1095 73 84 4710
Fragments arranged by size:
4710 1095 84 73
PfoI T'CCnGG_A
Cuts at: 2620 2620
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2141
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 2487 3532 2487
Size: 1045 4917
PstI C_TGCA'G
Cuts at: 830 3157 3169 3247 3314 830
Size: 2327 12 78 67 3478
Fragments arranged by size:
3478 2327 78 67 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SexAI A'CCwGG_T
Cuts at: 2086 2086
Size: 5962
SfcI C'TryA_G
Cuts at: 826 1045 2305 3153 3165 3243 3310 4445 5126
Size: 219 1260 848 12 78 67 1135 681
Cuts at: 5126 5317 826
Size: 191 1471
Fragments arranged by size:
1471 1260 1135 848 681 219 191 78
67 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2730 4066 4967 5229 820
Size: 197 926 787 1336 901 262 1553
Fragments arranged by size:
1553 1336 926 901 787 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 2815 3886 5
Size: 47 2763 1071 2081
Fragments arranged by size:
2763 2081 1071 47
StuI AGG'CCT
Cuts at: 1517 3086 1517
Size: 1569 4393
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2406 3003 3989 4006 4159 4344 4789
Size: 1255 826 597 986 17 153 185 445
Cuts at: 4789 325
Size: 1498
Fragments arranged by size:
1498 1255 986 826 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 2309 3041
Size: 274 80 33 51 1443 115 217 732
Cuts at: 3041 3116 4208 5920 96
Size: 75 1092 1712 138
Fragments arranged by size:
1712 1443 1092 732 274 217 138 115
80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 2347 2629 4091 2347
Size: 282 1462 4218
Fragments arranged by size:
4218 1462 282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI