Restriction map for
pFN21AB7252
(Circular) (Six-base) MAPSORT of: pFN21AB7252.seq Check: 1158 from: 1 to: 6148
pFN21AB7252 6148 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3228 3319 3228
Size: 91 6057
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4211 5595 5863 1632
Size: 2579 1384 268 1917
Fragments arranged by size:
2579 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 2634 1166
Size: 1468 4680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2475 2507 2778 2810 2475
Size: 32 271 32 5813
Fragments arranged by size:
5813 271 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2114 2542 2725 3891 5463 2114
Size: 428 183 1166 1572 2799
Fragments arranged by size:
2799 1572 1166 428 183
ApaI G_GGCC'C
Cuts at: 2054 2079 2558 2702 2858 3002 2054
Size: 25 479 144 156 144 5200
Fragments arranged by size:
5200 479 156 144 144 25
ApaLI G'TGCA_C
Cuts at: 2898 4205 5361 2898
Size: 1307 1156 3685
Fragments arranged by size:
3685 1307 1156
ApoI r'AATT_y
Cuts at: 1493 3143 3457 3683 3835 5944 1493
Size: 1650 314 226 152 2109 1697
Fragments arranged by size:
2109 1697 1650 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2616 3386 3473 1870
Size: 73 673 770 87 4545
Fragments arranged by size:
4545 770 673 87 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3469 4924 618
Size: 325 217 627 1682 1455 1842
Fragments arranged by size:
1842 1682 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2079 2135 2208 2331 2558 2702 2832
Size: 663 25 56 73 123 227 144 130
Cuts at: 2832 2858 2867 3002 3127 3243 3467 1391
Size: 26 9 135 125 116 224 4072
Fragments arranged by size:
4072 663 227 224 144 135 130 125
123 116 73 56 26 25 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BbvCI CC'TCA_GC
Cuts at: 3166 3166
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 2118 1548
Size: 570 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2964 6143 2964
Size: 3179 2969
BlpI GC'TnA_GC
Cuts at: 2103 3408 3565 2103
Size: 1305 157 4686
Fragments arranged by size:
4686 1305 157
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2079 2558 2702 2858 2902 3002 4209
Size: 264 25 479 144 156 44 100 1207
Cuts at: 4209 5365 1790
Size: 1156 2573
Fragments arranged by size:
2573 1207 1156 479 264 156 144 100
44 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 3228 3879
Size: 416 53 83 399 24 1211 600 651
Cuts at: 3879 4837 442
Size: 958 1753
Fragments arranged by size:
1753 1211 958 651 600 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2363 2666 2717 2783 3262 3291 4808
Size: 944 180 303 51 66 479 29 1517
Cuts at: 4808 1239
Size: 2579
Fragments arranged by size:
2579 1517 944 479 303 180 66 51
29
Bpu10I CC'TnA_GC
Cuts at: 3166 3166
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3262 4811 882
Size: 617 1763 1549 2219
Fragments arranged by size:
2219 1763 1549 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3955 4337 4972
Size: 53 83 186 936 476 1946 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1946 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2502 2505 2532 2894
Size: 30 165 30 1072 27 3 27 362
Cuts at: 2894 2897 2924 2927 2963 2993 3009 3039 1178
Size: 3 27 3 36 30 16 30 4287
Fragments arranged by size:
4287 1072 362 165 36 30 30 30
30 27 27 27 16 3 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2902 1186
Size: 1716 4432
BseYI C'CCAG_C
Cuts at: 2124 2330 2393 2567 2825 2930 5351 5847 2124
Size: 206 63 174 258 105 2421 496 2425
Fragments arranged by size:
2425 2421 496 258 206 174 105 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 1163
Size: 573 351 5224
Fragments arranged by size:
5224 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2902 3127 4209 4294 5365 2902
Size: 225 1082 85 1071 3685
Fragments arranged by size:
3685 1082 1071 225 85
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
BsmBI CGTCTCn'nnnn_
Cuts at: 2983 2983
Size: 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2079 2135 2208 2331 2558 2702
Size: 399 264 25 56 73 123 227 144
Cuts at: 2702 2832 2858 2867 2902 3002 3127 3243 3467
Size: 130 26 9 35 100 125 116 224
Cuts at: 3467 4209 4294 5365 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 264 227 224 144
130 125 123 116 100 85 73 56
35 26 25 9
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3228 3319 3503 844
Size: 2384 91 184 3489
Fragments arranged by size:
3489 2384 184 91
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2807 2401
Size: 406 5742
BstYI r'GATC_y
Cuts at: 1972 2964 3478 3845 4230 4247 5699 5785 5797
Size: 992 514 367 385 17 1452 86 12
Cuts at: 5797 6143 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 992 514 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2410 2659 3013 2410
Size: 249 354 5545
Fragments arranged by size:
5545 354 249
BtgI C'CryG_G
Cuts at: 513 1065 2872 513
Size: 552 1807 3789
Fragments arranged by size:
3789 1807 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3098 3664 4458 4478 3098
Size: 566 794 20 4768
Fragments arranged by size:
4768 794 566 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2809 3595 4484
Size: 54 1425 50 275 18 979 786 889
Cuts at: 4484 6113 8
Size: 1629 43
Fragments arranged by size:
1629 1425 979 889 786 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4078 1353
Size: 2725 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3125 3125
Size: 6148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2363 2666 2717 2783 3262 3291
Size: 393 551 180 303 51 66 479 29
Cuts at: 3291 4211 4808 5595 5863 1239
Size: 920 597 787 268 1524
Fragments arranged by size:
1524 920 787 597 551 479 393 303
268 180 66 51 29
EcoO109I rG'GnC_Cy
Cuts at: 2051 2058 2075 2076 2539 2555 2698 2699 2855
Size: 7 17 1 463 16 143 1 156
Cuts at: 2855 2999 3180 2051
Size: 144 181 5019
Fragments arranged by size:
5019 463 181 156 144 143 17 16
7 1 1
EcoRI G'AATT_C
Cuts at: 3143 3457 3835 3143
Size: 314 378 5456
Fragments arranged by size:
5456 378 314
EcoRV GAT'ATC
Cuts at: 2453 2453
Size: 6148
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 2806 5295 1678
Size: 1128 2489 2531
Fragments arranged by size:
2531 2489 1128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 2963 2995 4789
Size: 32 135 32 669 32 690 32 1794
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1794 690 669 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3234 5262 5446 1305
Size: 1929 2028 184 2007
Fragments arranged by size:
2028 2007 1929 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2811 10
Size: 54 1425 343 979 3347
Fragments arranged by size:
3347 1425 979 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2634 2951 3353 3359 4106 4465 4624
Size: 648 1468 317 402 6 747 359 159
Cuts at: 4624 518
Size: 2042
Fragments arranged by size:
2042 1468 747 648 402 359 317 159
6
MspA1I CmG'CkG
Cuts at: 1606 2124 2295 3564 4241 5389 5634 1606
Size: 518 171 1269 677 1148 245 2120
Fragments arranged by size:
2120 1269 1148 677 518 245 171
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5596
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 2592 3506 5051 1132
Size: 97 1363 914 1545 2229
Fragments arranged by size:
2229 1545 1363 914 97
PasI CC'CwG_GG
Cuts at: 1348 2714 2851 1348
Size: 1366 137 4645
Fragments arranged by size:
4645 1366 137
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2097 1108
Size: 989 5159
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2475 2507 4217 4249 5730 5762 2475
Size: 32 1710 32 1481 32 2861
Fragments arranged by size:
2861 1710 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 3180 2058
Size: 1122 5026
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspOMI G'GGCC_C
Cuts at: 2050 2075 2554 2698 2854 2998 2050
Size: 25 479 144 156 144 5200
Fragments arranged by size:
5200 479 156 144 144 25
PstI C_TGCA'G
Cuts at: 830 2317 2697 3500 830
Size: 1487 380 803 3478
Fragments arranged by size:
3478 1487 803 380
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2124 2124
Size: 6148
SacI G_AGCT'C
Cuts at: 3127 3127
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SbfI CC_TGCA'GG
Cuts at: 2697 3500 2697
Size: 803 5345
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2313 2693 3184 3496 4631 5312 5503
Size: 219 1268 380 491 312 1135 681 191
Cuts at: 5503 826
Size: 1471
Fragments arranged by size:
1471 1268 1135 681 491 380 312 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 2618 3475 1872
Size: 746 857 4545
Fragments arranged by size:
4545 857 746
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2035 2208 3324 3587 513
Size: 552 970 173 1116 263 3074
Fragments arranged by size:
3074 1116 970 552 263 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3304 4175 4192 4345 4530 4975 325
Size: 1255 1724 871 17 153 185 445 1498
Fragments arranged by size:
1724 1498 1255 871 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 4394 6106
Size: 274 80 33 51 1443 906 1511 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1712 1511 1443 906 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 2894 2926 3009 3041 6108 6140 2502
Size: 32 360 32 83 32 3067 32 2510
Fragments arranged by size:
3067 2510 360 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2975 1316
Size: 1659 4489
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2401 2641 2848 3241 2338
Size: 63 240 207 393 5245
Fragments arranged by size:
5245 393 240 207 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 2616 3473 1870
Size: 746 857 4545
Fragments arranged by size:
4545 857 746
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI