Restriction map for
pFN21AB7250
(Circular) (Six-base) MAPSORT of: pFN21AB7250.seq Check: 5080 from: 1 to: 5149
pFN21AB7250 5149 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2354 2354
Size: 5149
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2959 278
Size: 53 83 186 936 1423 2468
Fragments arranged by size:
2468 1423 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2492 1937
Size: 555 4594
Acc65I G'GTAC_C
Cuts at: 2470 2470
Size: 5149
AclI AA'CG_TT
Cuts at: 1563 3276 3649 1563
Size: 1713 373 3063
Fragments arranged by size:
3063 1713 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 4596 4864 1632
Size: 1580 1384 268 1917
Fragments arranged by size:
1917 1580 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4952
AflIII A'CryG_T
Cuts at: 1203 4048 5025 1203
Size: 2845 977 1327
Fragments arranged by size:
2845 1327 977
AgeI A'CCGG_T
Cuts at: 3980 3980
Size: 5149
AhdI GACnn_n'nnGTC
Cuts at: 2177 3878 2177
Size: 1701 3448
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5149
AlwNI CAG_nnn'CTG
Cuts at: 2320 2417 2892 4464 2320
Size: 97 475 1572 3005
Fragments arranged by size:
3005 1572 475 97
ApaI G_GGCC'C
Cuts at: 2200 2279 2297 2200
Size: 79 18 5052
Fragments arranged by size:
5052 79 18
ApaLI G'TGCA_C
Cuts at: 3206 4362 3206
Size: 1156 3993
ApoI r'AATT_y
Cuts at: 1493 2458 2684 2836 4945 1493
Size: 965 226 152 2109 1697
Fragments arranged by size:
2109 1697 965 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3703 160
Size: 872 2671 1606
Fragments arranged by size:
2671 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
AvaI C'yCGr_G
Cuts at: 1870 1943 2272 2290 2474 1870
Size: 73 329 18 184 4545
Fragments arranged by size:
4545 329 184 73 18
BamHI G'GATC_C
Cuts at: 2013 2479 2846 2013
Size: 466 367 4316
Fragments arranged by size:
4316 466 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2157 2216 2342 2470 3925
Size: 325 217 627 370 59 126 128 1455
Cuts at: 3925 618
Size: 1842
Fragments arranged by size:
1842 1455 627 370 325 217 128 126
59
BanII G_rGCy'C
Cuts at: 1391 2121 2135 2200 2279 2297 2468 1391
Size: 730 14 65 79 18 171 4072
Fragments arranged by size:
4072 730 171 79 65 18 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2290 928
Size: 971 391 3787
Fragments arranged by size:
3787 971 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3340 3374 1903
Size: 34 1403 34 3678
Fragments arranged by size:
3678 1403 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3041 4251 3041
Size: 1210 3939
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5149
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2204 2225 3760
Size: 107 122 71 631 605 532 21 1535
Cuts at: 3760 136
Size: 1525
Fragments arranged by size:
1535 1525 631 605 532 122 107 71
21
BglII A'GATC_T
Cuts at: 5144 5144
Size: 5149
BlpI GC'TnA_GC
Cuts at: 2566 2566
Size: 5149
Bme1580I G_kGCm'C
Cuts at: 1790 2200 2279 2297 2345 3210 4366 1790
Size: 410 79 18 48 865 1156 2573
Fragments arranged by size:
2573 1156 865 410 79 48 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5149
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2880 3838 442
Size: 416 53 83 399 24 1463 958 1753
Fragments arranged by size:
1753 1463 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 3809 1239
Size: 947 1623 2579
Fragments arranged by size:
2579 1623 947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3274 4139 4437 3274
Size: 865 298 3986
Fragments arranged by size:
3986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3812 882
Size: 617 2313 2219
Fragments arranged by size:
2313 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2940 493
Size: 711 1736 2702
Fragments arranged by size:
2702 1736 711
BsaBI GATnn'nnATC
Cuts at: 5143 5143
Size: 5149
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2956 3338 3973 275
Size: 53 83 186 936 1423 382 635 1451
Fragments arranged by size:
1451 1423 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3582 3980 4254 4401 4812 3582
Size: 398 274 147 411 3919
Fragments arranged by size:
3919 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4924
Fragments arranged by size:
4924 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4299
BseYI C'CCAG_C
Cuts at: 2122 2313 2338 2426 4352 4848 2122
Size: 191 25 88 1926 496 2423
Fragments arranged by size:
2423 1926 496 191 88 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 3937
Fragments arranged by size:
3937 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 2397 2401 2599 3360 4388
Size: 275 186 24 372 4 198 761 1028
Cuts at: 4388 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 372 275 198 186 24
4
BsiHKAI G_wGCw'C
Cuts at: 3210 3295 4366 3210
Size: 85 1071 3993
Fragments arranged by size:
3993 1071 85
BsmI GAATG_Cn'
Cuts at: 2660 2753 2660
Size: 93 5056
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2135 2200 2279 2297 2345 2468
Size: 399 331 14 65 79 18 48 123
Cuts at: 2468 3210 3295 4366 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 123 85 79
65 48 18 14
BspEI T'CCGG_A
Cuts at: 4812 4812
Size: 5149
BspHI T'CATG_A
Cuts at: 3038 4768 3038
Size: 1730 3419
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2354 2504 844
Size: 1510 150 3489
Fragments arranged by size:
3489 1510 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3036 1394
Size: 429 1213 3507
Fragments arranged by size:
3507 1213 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3644 3818 65
Size: 1183 2396 174 1396
Fragments arranged by size:
2396 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 2392 2396 2400 3793 3980 1700
Size: 110 409 173 4 4 1393 187 2869
Fragments arranged by size:
2869 1393 409 187 173 110 4 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5149
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5149
BssSI C'ACGA_G
Cuts at: 3209 4221 3209
Size: 1012 4137
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5149
BstBI TT'CG_AA
Cuts at: 3953 3953
Size: 5149
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 2444
Size: 5149
BstYI r'GATC_y
Cuts at: 1972 2013 2479 2846 3231 3248 4700 4786 4798
Size: 41 466 367 385 17 1452 86 12
Cuts at: 4798 5144 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 466 385 367 346 86 41
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3658
Fragments arranged by size:
3658 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4410
Fragments arranged by size:
4410 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3459 3479 2665
Size: 794 20 4335
Fragments arranged by size:
4335 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5114
DraI TTT'AAA
Cuts at: 2453 2453
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4156 809
Size: 507 2840 1802
Fragments arranged by size:
2840 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2260 2394
Size: 54 1425 50 275 18 192 238 134
Cuts at: 2394 2398 2596 3485 5114 8
Size: 4 198 889 1629 43
Fragments arranged by size:
1629 1425 889 275 238 198 192 134
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2022 2394 2398 2596 1537
Size: 275 210 372 4 198 4090
Fragments arranged by size:
4090 372 275 210 198 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3079 1353
Size: 1726 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3736 4110 4256 387
Size: 1064 138 2147 374 146 1280
Fragments arranged by size:
2147 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 3212 3809 4596 4864 1239
Size: 393 554 1026 597 787 268 1524
Fragments arranged by size:
1524 1026 787 597 554 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2092 2197 2276 2294 2304 2092
Size: 105 79 18 10 4937
Fragments arranged by size:
4937 105 79 18 10
EcoRI G'AATT_C
Cuts at: 2458 2836 2458
Size: 378 4771
FseI GG_CCGG'CC
Cuts at: 2400 2400
Size: 5149
FspI TGC'GCA
Cuts at: 3655 3655
Size: 5149
HaeII r_GCGC'y
Cuts at: 1678 4296 1678
Size: 2618 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2163 2195 3790 3822 3864
Size: 32 135 32 591 32 1595 32 42
Cuts at: 3864 3896 1373
Size: 32 2626
Fragments arranged by size:
2626 1595 591 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2493 2739 669
Size: 988 281 555 246 3079
Fragments arranged by size:
3079 988 555 281 246
HindIII A'AGCT_T
Cuts at: 748 2186 748
Size: 1438 3711
HpaI GTT'AAC
Cuts at: 2739 2739
Size: 5149
KpnI G_GTAC'C
Cuts at: 2474 2474
Size: 5149
MfeI C'AATT_G
Cuts at: 2748 2748
Size: 5149
MluI A'CGCG_T
Cuts at: 5025 5025
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4263 4447 1305
Size: 2958 184 2007
Fragments arranged by size:
2958 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3327
Fragments arranged by size:
3327 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 3107 3466 3625 518
Size: 648 1276 665 359 159 2042
Fragments arranged by size:
2042 1276 665 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2565 3242 4390 4635 1606
Size: 959 677 1148 245 2120
Fragments arranged by size:
2120 1148 959 677 245
NaeI GCC'GGC
Cuts at: 2221 2398 2402 2221
Size: 177 4 4968
Fragments arranged by size:
4968 177 4
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3658
Fragments arranged by size:
3658 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5149
NgoMIV G'CCGG_C
Cuts at: 2219 2396 2400 2219
Size: 177 4 4968
Fragments arranged by size:
4968 177 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5149
NotI GC'GGCC_GC
Cuts at: 2596 2596
Size: 5149
NspI r_CATG'y
Cuts at: 1132 1229 2507 4052 1132
Size: 97 1278 1545 2229
Fragments arranged by size:
2229 1545 1278 97
PasI CC'CwG_GG
Cuts at: 1348 2029 2112 1348
Size: 681 83 4385
Fragments arranged by size:
4385 681 83
PciI A'CATG_T
Cuts at: 4048 4048
Size: 5149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5149
PfoI T'CCnGG_A
Cuts at: 2250 2250
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2453 2453
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 4731 4763 3218
Size: 32 1481 32 3604
Fragments arranged by size:
3604 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2304 2092
Size: 212 4937
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5149
PsiI TTA'TAA
Cuts at: 2719 2719
Size: 5149
PspOMI G'GGCC_C
Cuts at: 2196 2275 2293 2196
Size: 79 18 5052
Fragments arranged by size:
5052 79 18
PstI C_TGCA'G
Cuts at: 830 2058 2151 2501 830
Size: 1228 93 350 3478
Fragments arranged by size:
3478 1228 350 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5149
SalI G'TCGA_C
Cuts at: 1936 2491 1936
Size: 555 4594
SanDI GG'GwC_CC
Cuts at: 2092 2092
Size: 5149
SbfI CC_TGCA'GG
Cuts at: 2501 2501
Size: 5149
ScaI AGT'ACT
Cuts at: 3397 5109 3397
Size: 1712 3437
SfcI C'TryA_G
Cuts at: 826 1045 2054 2147 2497 3632 4313 4504 826
Size: 219 1009 93 350 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 350 219 191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5149
SmaI CCC'GGG
Cuts at: 1872 2476 1872
Size: 604 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3253 4154 4416 820
Size: 197 926 1310 901 262 1553
Fragments arranged by size:
1553 1310 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2940 493
Size: 2447 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5149
SphI G_CATG'C
Cuts at: 1132 2507 1132
Size: 1375 3774
SspI AAT'ATT
Cuts at: 5 52 3073 5
Size: 47 3021 2081
Fragments arranged by size:
3021 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5149
StyI C'CwwG_G
Cuts at: 513 1065 2004 2588 513
Size: 552 939 584 3074
Fragments arranged by size:
3074 939 584 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3176 3193 3346 3531 3976 325
Size: 1255 1596 17 153 185 445 1498
Fragments arranged by size:
1596 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3395 5107 96
Size: 274 80 33 51 1443 1418 1712 138
Fragments arranged by size:
1712 1443 1418 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5109 5141 5109
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5149
XbaI T'CTAG_A
Cuts at: 2485 2485
Size: 5149
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5149
XmaI C'CCGG_G
Cuts at: 1870 2474 1870
Size: 604 4545
XmnI GAAnn'nnTTC
Cuts at: 3278 3278
Size: 5149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2957 276
Size: 53 83 186 936 1423 2468
Fragments arranged by size:
2468 1423 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI