Restriction map for
pFN21AB7245
(Circular) (Six-base) MAPSORT of: pFN21AB7245.seq Check: 8926 from: 1 to: 5626
pFN21AB7245 5626 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2729 2729
Size: 5626
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 2969 1937
Size: 169 863 4594
Fragments arranged by size:
4594 863 169
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 3689 5073 5341 1632
Size: 579 1478 1384 268 1917
Fragments arranged by size:
1917 1478 1384 579 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 2450 1166
Size: 1284 4342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 2234 3369 4941 2234
Size: 1135 1572 2919
Fragments arranged by size:
2919 1572 1135
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2951 1870
Size: 73 121 887 4545
Fragments arranged by size:
4545 887 121 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2013 2046 2013
Size: 33 5593
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2947 4402 618
Size: 325 217 627 543 617 1455 1842
Fragments arranged by size:
1842 1455 627 617 543 325 217
BanII G_rGCy'C
Cuts at: 1391 2055 2073 2945 1391
Size: 664 18 872 4072
Fragments arranged by size:
4072 872 664 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2332 928
Size: 971 136 297 4222
Fragments arranged by size:
4222 971 297 136
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 5626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2304 2601 5621 2304
Size: 297 3020 2309
Fragments arranged by size:
3020 2309 297
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 3687 4843 1790
Size: 1897 1156 2573
Fragments arranged by size:
2573 1897 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2609 4286 1239
Size: 818 552 1677 2579
Fragments arranged by size:
2579 1677 818 552
Bpu10I CC'TnA_GC
Cuts at: 2678 2678
Size: 5626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3751 4616 4914 2582
Size: 1169 865 298 3294
Fragments arranged by size:
3294 1169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2025 3417 493
Size: 711 821 1392 2702
Fragments arranged by size:
2702 1392 821 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2448 2478 1178
Size: 30 165 30 1045 30 4326
Fragments arranged by size:
4326 1045 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2633 1186
Size: 1063 384 4179
Fragments arranged by size:
4179 1063 384
BseYI C'CCAG_C
Cuts at: 2163 4829 5325 2163
Size: 2666 496 2464
Fragments arranged by size:
2666 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 2707 1163
Size: 573 818 153 4082
Fragments arranged by size:
4082 818 573 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 3687 3772 4843 2073
Size: 1614 85 1071 2856
Fragments arranged by size:
2856 1614 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2073 2945 3687 3772 4843 1391
Size: 399 265 18 872 742 85 1071 2174
Fragments arranged by size:
2174 1071 872 742 399 265 85 18
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 2409 2835 3515 5245 2409
Size: 426 680 1730 2790
Fragments arranged by size:
2790 1730 680 426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 2981 844
Size: 1885 252 3489
Fragments arranged by size:
3489 1885 252
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2373 4121 4295 65
Size: 1183 1125 1748 174 1396
Fragments arranged by size:
1748 1396 1183 1125 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2916 4270 4457 1700
Size: 110 1106 1354 187 2869
Fragments arranged by size:
2869 1354 1106 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2452 2524 2122
Size: 330 72 5224
Fragments arranged by size:
5224 330 72
BstYI r'GATC_y
Cuts at: 1972 2304 2601 2956 3323 3708 3725 5177 5263
Size: 332 297 355 367 385 17 1452 86
Cuts at: 5263 5275 5621 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 355 346 332 297
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2870 2870
Size: 5626
BtgI C'CryG_G
Cuts at: 513 1065 2004 2334 513
Size: 552 939 330 3805
Fragments arranged by size:
3805 939 552 330
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 2232 2498 2582 2901 3142 3936 3956 2232
Size: 266 84 319 241 794 20 3902
Fragments arranged by size:
3902 794 319 266 241 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 3073 3962
Size: 54 1425 50 275 18 994 249 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 994 889 275 249 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2230 3556 1353
Size: 877 1326 3423
Fragments arranged by size:
3423 1326 877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 5626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2211 2609 3689 4286 5073 5341
Size: 393 425 154 398 1080 597 787 268
Cuts at: 5341 1239
Size: 1524
Fragments arranged by size:
1524 1080 787 597 425 398 393 268
154
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2448 2480 2671
Size: 32 135 32 674 32 170 32 191
Cuts at: 2671 2703 4267 4299 4341 4373 1373
Size: 32 1564 32 42 32 2626
Fragments arranged by size:
2626 1564 674 191 170 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2810 748
Size: 2062 3564
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 4740 4924 1305
Size: 1326 2109 184 2007
Fragments arranged by size:
2109 2007 1326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2147 2177 2408 2450 2669 2738
Size: 648 954 27 30 231 42 219 69
Cuts at: 2738 2834 3584 3943 4102 518
Size: 96 750 359 159 2042
Fragments arranged by size:
2042 954 750 648 359 231 219 159
96 69 42 30 27
MspA1I CmG'CkG
Cuts at: 1606 2482 3042 3719 4867 5112 1606
Size: 876 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 876 677 560 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2334 513
Size: 552 939 330 3805
Fragments arranged by size:
3805 939 552 330
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2887 2984 4529 1132
Size: 97 1658 97 1545 2229
Fragments arranged by size:
2229 1658 1545 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 4411
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PmlI CAC'GTG
Cuts at: 2025 2025
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2482 2482
Size: 5626
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 2556 3874 5586 2556
Size: 1318 1712 2596
Fragments arranged by size:
2596 1712 1318
SfcI C'TryA_G
Cuts at: 826 1045 2974 4109 4790 4981 826
Size: 219 1929 1135 681 191 1471
Fragments arranged by size:
1929 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3730 4631 4893 820
Size: 197 926 654 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2004 2334 3065 513
Size: 552 939 330 731 3074
Fragments arranged by size:
3074 939 731 552 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2416 2440 2744 3653 3670 3823
Size: 1255 611 225 24 304 909 17 153
Cuts at: 3823 4008 4453 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 909 611 445 304 225 185
153 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2554 3872
Size: 274 80 33 51 1443 204 373 1318
Cuts at: 3872 5584 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1318 373 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 2039 1316
Size: 723 4903
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2248
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI