Restriction map for
pFN21AB7243
(Circular) (Six-base) MAPSORT of: pFN21AB7243.seq Check: 3601 from: 1 to: 6079
pFN21AB7243 6079 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 3024 3422 1937
Size: 784 303 398 4594
Fragments arranged by size:
4594 784 398 303
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4142 5526 5794 1632
Size: 2510 1384 268 1917
Fragments arranged by size:
2510 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2163 4978 5955 1203
Size: 960 2815 977 1327
Fragments arranged by size:
2815 1327 977 960
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 2105 2213 2648 1166
Size: 939 108 435 4597
Fragments arranged by size:
4597 939 435 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2408 1184
Size: 1224 4855
AlwNI CAG_nnn'CTG
Cuts at: 2188 2230 2452 2761 3822 5394 2188
Size: 42 222 309 1061 1572 2873
Fragments arranged by size:
2873 1572 1061 309 222 42
ApaI G_GGCC'C
Cuts at: 2663 2861 2663
Size: 198 5881
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 3388 3614 3766 5875 1493
Size: 1895 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 3173 3404 1870
Size: 73 110 1120 231 4545
Fragments arranged by size:
4545 1120 231 110 73
BamHI G'GATC_C
Cuts at: 2580 3409 3776 2580
Size: 829 367 4883
Fragments arranged by size:
4883 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2480 2510 2609 3400 4855
Size: 325 217 627 693 30 99 791 1455
Cuts at: 4855 618
Size: 1842
Fragments arranged by size:
1842 1455 791 693 627 325 217 99
30
BanII G_rGCy'C
Cuts at: 1391 2156 2490 2663 2861 3398 1391
Size: 765 334 173 198 537 4072
Fragments arranged by size:
4072 765 537 334 198 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 4984
Fragments arranged by size:
4984 971 124
BbvCI CC'TCA_GC
Cuts at: 2828 2828
Size: 6079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2755 2789 4270 4304 1903
Size: 34 818 34 1481 34 3678
Fragments arranged by size:
3678 1481 818 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2304 2501 2703
Size: 107 122 71 631 605 632 197 202
Cuts at: 2703 3332 4690 136
Size: 629 1358 1525
Fragments arranged by size:
1525 1358 632 631 629 605 202 197
122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 2396 2762 2900 3496 2396
Size: 366 138 596 4979
Fragments arranged by size:
4979 596 366 138
Bme1580I G_kGCm'C
Cuts at: 1790 2614 2663 2861 4140 5296 1790
Size: 824 49 198 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 824 198 49
BmgBI CAC'GTC
Cuts at: 1318 2166 1318
Size: 848 5231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3227 3810 4768
Size: 416 53 83 399 24 1810 583 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1810 1753 958 583 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2592 2964 2968 4739 1239
Size: 1353 372 4 1771 2579
Fragments arranged by size:
2579 1771 1353 372 4
Bpu10I CC'TnA_GC
Cuts at: 2828 2828
Size: 6079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4204 5069 5367 4204
Size: 865 298 4916
Fragments arranged by size:
4916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2065 2466 3152 4742 882
Size: 617 566 401 686 1590 2219
Fragments arranged by size:
2219 1590 686 617 566 401
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3291 3886 4268 4903
Size: 53 83 186 936 1758 595 382 635
Cuts at: 4903 275
Size: 1451
Fragments arranged by size:
1758 1451 936 635 595 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2590 4512 4910 5184 5331 5742 2590
Size: 1922 398 274 147 411 2927
Fragments arranged by size:
2927 1922 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 2937 2967 1178
Size: 30 165 30 664 30 840 30 4290
Fragments arranged by size:
4290 840 664 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2146 2251 2330 2609 2762 3170 1186
Size: 889 71 105 79 279 153 408 4095
Fragments arranged by size:
4095 889 408 279 153 105 79 71
BseYI C'CCAG_C
Cuts at: 2044 2343 3167 5282 5778 2044
Size: 299 824 2115 496 2345
Fragments arranged by size:
2345 2115 824 496 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2265 3116 1163
Size: 573 529 851 4126
Fragments arranged by size:
4126 851 573 529
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3255 3529 4290 5318 1540
Size: 275 186 1254 274 761 1028 2301
Fragments arranged by size:
2301 1254 1028 761 275 274 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2490 2681 4140 4225 5296 2112
Size: 378 191 1459 85 1071 2895
Fragments arranged by size:
2895 1459 1071 378 191 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
BsmBI CGTCTCn'nnnn_
Cuts at: 2959 2959
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2156 2490 2614 2663 2681 2861
Size: 399 322 44 334 124 49 18 180
Cuts at: 2861 3398 4140 4225 5296 1391
Size: 537 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 334 322 180
124 85 49 44 18
BspEI T'CCGG_A
Cuts at: 2590 5742 2590
Size: 3152 2927
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 3434 844
Size: 1173 1417 3489
Fragments arranged by size:
3489 1417 1173
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2457 3262 4723 4910 1700
Size: 110 647 805 1461 187 2869
Fragments arranged by size:
2869 1461 805 647 187 110
BsrGI T'GTAC_A
Cuts at: 96 3114 96
Size: 3018 3061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 2774 3134 4139 5151 2774
Size: 360 1005 1012 3702
Fragments arranged by size:
3702 1012 1005 360
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstXI CCAn_nnnn'nTGG
Cuts at: 2368 2368
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2580 3409 3776 4161 4178 5630 5716 5728
Size: 608 829 367 385 17 1452 86 12
Cuts at: 5728 6074 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 829 608 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3106 3106
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 2004 2376 2466 513
Size: 552 939 372 90 4126
Fragments arranged by size:
4126 939 552 372 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4389 4409 3595
Size: 794 20 5265
Fragments arranged by size:
5265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DraIII CAC_nnn'GTG
Cuts at: 2879 2879
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2717 5086 809
Size: 507 1401 2369 1802
Fragments arranged by size:
2369 1802 1401 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 3252 3300
Size: 54 1425 50 275 18 663 759 48
Cuts at: 3300 3526 4415 6044 8
Size: 226 889 1629 43
Fragments arranged by size:
1629 1425 889 759 663 275 226 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3252 3526 1537
Size: 275 1440 274 4090
Fragments arranged by size:
4090 1440 275 274
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 3368 4009 1353
Size: 824 1191 641 3423
Fragments arranged by size:
3423 1191 824 641
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2488 2488
Size: 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2592 2964 2968 4142 4739 5526 5794
Size: 393 960 372 4 1174 597 787 268
Cuts at: 5794 1239
Size: 1524
Fragments arranged by size:
1524 1174 960 787 597 393 372 268
4
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 3134 2093
Size: 1041 5038
EcoO109I rG'GnC_Cy
Cuts at: 2049 2227 2249 2449 2628 2659 2660 2049
Size: 178 22 200 179 31 1 5468
Fragments arranged by size:
5468 200 179 178 31 22 1
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 2324 5226 1678
Size: 646 2902 2531
Fragments arranged by size:
2902 2531 646
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2565 2572 2597
Size: 32 135 32 566 32 395 7 25
Cuts at: 2597 2604 2937 2969 4720 4752 4794 4826 1373
Size: 7 333 32 1751 32 42 32 2626
Fragments arranged by size:
2626 1751 566 395 333 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 3423 3669 669
Size: 988 281 595 890 246 3079
Fragments arranged by size:
3079 988 890 595 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2501 2733 5193 5377 1305
Size: 1196 232 2460 184 2007
Fragments arranged by size:
2460 2007 1196 232 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3302 10
Size: 54 1425 343 1470 2787
Fragments arranged by size:
2787 1470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 2213 2648 4037 4396 4555 518
Size: 648 939 108 435 1389 359 159 2042
Fragments arranged by size:
2042 1389 939 648 435 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2832 2863 2899 2988 3037 3084 3495 4172
Size: 1226 31 36 89 49 47 411 677
Cuts at: 4172 5320 5565 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1226 1148 677 411 245 89 49
47 36 31
NaeI GCC'GGC
Cuts at: 3264 3264
Size: 6079
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NgoMIV G'CCGG_C
Cuts at: 3262 3262
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3229 3437 4982 1132
Size: 97 2000 208 1545 2229
Fragments arranged by size:
2229 2000 1545 208 97
PasI CC'CwG_GG
Cuts at: 1348 2854 3071 1348
Size: 1506 217 4356
Fragments arranged by size:
4356 1506 217
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2230 2452 1108
Size: 1122 222 4735
Fragments arranged by size:
4735 1122 222
PfoI T'CCnGG_A
Cuts at: 2015 2964 2015
Size: 949 5130
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 2449
Size: 6079
PshAI GACnn'nnGTC
Cuts at: 1613 2552 1613
Size: 939 5140
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2659 2857 2659
Size: 198 5881
PspXI vC'TCGA_Gb
Cuts at: 3173 3173
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2304 3431 830
Size: 1474 1127 3478
Fragments arranged by size:
3478 1474 1127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2988 2988
Size: 6079
SacI G_AGCT'C
Cuts at: 2490 2490
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 3368 3368
Size: 6079
SbfI CC_TGCA'GG
Cuts at: 2304 3431 2304
Size: 1127 4952
ScaI AGT'ACT
Cuts at: 2561 4327 6039 2561
Size: 1766 1712 2601
Fragments arranged by size:
2601 1766 1712
SfcI C'TryA_G
Cuts at: 826 1045 2300 2713 3427 4562 5243 5434 826
Size: 219 1255 413 714 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 714 681 413 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2501 2501
Size: 6079
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3173 4183 5084 5346 820
Size: 197 926 1230 1010 901 262 1553
Fragments arranged by size:
1553 1230 1010 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 2067 3111 1517
Size: 550 1044 4485
Fragments arranged by size:
4485 1044 550
StyI C'CwwG_G
Cuts at: 513 1065 2004 3351 3518 513
Size: 552 939 1347 167 3074
Fragments arranged by size:
3074 1347 939 552 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 2459 2597 2651 2696 3286 4106
Size: 1255 794 85 138 54 45 590 820
Cuts at: 4106 4123 4276 4461 4906 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 820 794 590 445 185 153
138 85 54 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 3114 4325
Size: 274 80 33 51 1443 582 555 1211
Cuts at: 4325 6037 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1211 582 555 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2349 2381 2865 2897 6039 6071 2067
Size: 32 250 32 484 32 3142 32 2075
Fragments arranged by size:
3142 2075 484 250 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3020 1316
Size: 1704 4375
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2851 2851
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 3173 1943
Size: 1230 4849
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI