Restriction map for
pFN21AB7239
(Circular) (Six-base) MAPSORT of: pFN21AB7239.seq Check: 6975 from: 1 to: 5377
pFN21AB7239 5377 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2150 2258 2150
Size: 108 5269
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2075 2182 2720 1937
Size: 138 107 538 4594
Fragments arranged by size:
4594 538 138 107
Acc65I G'GTAC_C
Cuts at: 2103 2698 2103
Size: 595 4782
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 3440 4824 5092 1632
Size: 773 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 773 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2137 1184
Size: 953 4424
AlwNI CAG_nnn'CTG
Cuts at: 2569 3120 4692 2569
Size: 551 1572 3254
Fragments arranged by size:
3254 1572 551
ApaLI G'TGCA_C
Cuts at: 3434 4590 3434
Size: 1156 4221
ApoI r'AATT_y
Cuts at: 1493 2686 2912 3064 5173 1493
Size: 1193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2702 1870
Size: 73 759 4545
Fragments arranged by size:
4545 759 73
BamHI G'GATC_C
Cuts at: 2409 2707 3074 2409
Size: 298 367 4712
Fragments arranged by size:
4712 367 298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2103 2162 2698 4153 618
Size: 325 217 627 316 59 536 1455 1842
Fragments arranged by size:
1842 1455 627 536 325 316 217 59
BanII G_rGCy'C
Cuts at: 1391 2696 1391
Size: 1305 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 3784
Fragments arranged by size:
3784 971 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3568 3602 1903
Size: 34 1631 34 3678
Fragments arranged by size:
3678 1631 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3988 136
Size: 107 122 71 631 605 2316 1525
Fragments arranged by size:
2316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2380 5372 2380
Size: 2992 2385
BlpI GC'TnA_GC
Cuts at: 2794 2794
Size: 5377
Bme1580I G_kGCm'C
Cuts at: 1790 3438 4594 1790
Size: 1648 1156 2573
Fragments arranged by size:
2573 1648 1156
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2675 4037 1239
Size: 1436 1362 2579
Fragments arranged by size:
2579 1436 1362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 3566 4201 275
Size: 53 83 186 936 1651 382 635 1451
Fragments arranged by size:
1651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2236 2640 3810 4208 4482 4629 5040 2236
Size: 404 1170 398 274 147 411 2573
Fragments arranged by size:
2573 1170 411 404 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 3988
Fragments arranged by size:
3988 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5377
BseYI C'CCAG_C
Cuts at: 2614 4580 5076 2614
Size: 1966 496 2915
Fragments arranged by size:
2915 1966 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 1163
Size: 573 747 4057
Fragments arranged by size:
4057 747 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3438 3523 4594 3438
Size: 85 1071 4221
Fragments arranged by size:
4221 1071 85
BsmI GAATG_Cn'
Cuts at: 2195 2888 2981 2195
Size: 693 93 4591
Fragments arranged by size:
4591 693 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2696 3438 3523 4594 1391
Size: 399 906 742 85 1071 2174
Fragments arranged by size:
2174 1071 906 742 399 85
BspEI T'CCGG_A
Cuts at: 2640 5040 2640
Size: 2400 2977
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 2258 2577 2732 844
Size: 1306 108 319 155 3489
Fragments arranged by size:
3489 1306 319 155 108
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2631 3872 4046 65
Size: 1183 1383 1241 174 1396
Fragments arranged by size:
1396 1383 1241 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4021 4208 1700
Size: 110 2211 187 2869
Fragments arranged by size:
2869 2211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5377
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5377
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2644 2173
Size: 471 4906
BstYI r'GATC_y
Cuts at: 1972 2380 2409 2707 3074 3459 3476 4928 5014
Size: 408 29 298 367 385 17 1452 86
Cuts at: 5014 5026 5372 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 408 385 367 346 298 86
29 17 12
BstZ17I GTA'TAC
Cuts at: 2183 2183
Size: 5377
BtgI C'CryG_G
Cuts at: 513 1065 2556 513
Size: 552 1491 3334
Fragments arranged by size:
3334 1491 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 2436 1554
Size: 882 4495
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 3713 5342
Size: 54 1425 50 275 18 994 889 1629
Cuts at: 5342 8
Size: 43
Fragments arranged by size:
1629 1425 994 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3307 1353
Size: 1954 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2591 3964 4338 4484 387
Size: 1064 138 1002 1373 374 146 1280
Fragments arranged by size:
1373 1280 1064 1002 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2675 3440 4037 4824 5092 1239
Size: 393 773 270 765 597 787 268 1524
Fragments arranged by size:
1524 787 773 765 597 393 270 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2417 2417
Size: 5377
EcoO109I rG'GnC_Cy
Cuts at: 2095 2138 2095
Size: 43 5334
EcoRI G'AATT_C
Cuts at: 2686 3064 2686
Size: 378 4999
FspI TGC'GCA
Cuts at: 3883 3883
Size: 5377
HaeII r_GCGC'y
Cuts at: 1678 2428 2485 4524 1678
Size: 750 57 2039 2531
Fragments arranged by size:
2531 2039 750 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 4018 4050 4092
Size: 32 135 32 965 32 1449 32 42
Cuts at: 4092 4124 1373
Size: 32 2626
Fragments arranged by size:
2626 1449 965 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2721 2967 669
Size: 988 281 783 246 3079
Fragments arranged by size:
3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KpnI G_GTAC'C
Cuts at: 2107 2702 2107
Size: 595 4782
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4491 4675 1305
Size: 3186 184 2007
Fragments arranged by size:
3186 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3555
Fragments arranged by size:
3555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2119 2642 3335 3694 3853 518
Size: 648 953 523 693 359 159 2042
Fragments arranged by size:
2042 953 693 648 523 359 159
MspA1I CmG'CkG
Cuts at: 1606 2614 2793 3470 4618 4863 1606
Size: 1008 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 1008 677 245 179
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4825
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2118 2735 4280 1132
Size: 97 889 617 1545 2229
Fragments arranged by size:
2229 1545 889 617 97
PasI CC'CwG_GG
Cuts at: 1348 2419 1348
Size: 1071 4306
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5377
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 5377
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2138 2095
Size: 43 5334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2480 2512 2480
Size: 32 5345
PstI C_TGCA'G
Cuts at: 830 2161 2729 830
Size: 1331 568 3478
Fragments arranged by size:
3478 1331 568
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
RsrII CG'GwC_CG
Cuts at: 2545 2545
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2157 2725 3860 4541 4732 826
Size: 219 1112 568 1135 681 191 1471
Fragments arranged by size:
1471 1135 1112 681 568 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3481 4382 4644 820
Size: 197 926 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2118 2735 1132
Size: 986 617 3774
Fragments arranged by size:
3774 986 617
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5377
StyI C'CwwG_G
Cuts at: 513 1065 2131 2172 2816 513
Size: 552 1066 41 644 3074
Fragments arranged by size:
3074 1066 644 552 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2319 2432 2489 3404 3421 3574 3759
Size: 1255 739 113 57 915 17 153 185
Cuts at: 3759 4204 325
Size: 445 1498
Fragments arranged by size:
1498 1255 915 739 445 185 153 113
57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2454 2466 3623
Size: 274 80 33 51 1443 477 12 1157
Cuts at: 3623 5335 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1157 477 274 138 80 51
33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5337 5369 5337
Size: 32 5345
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI RsrII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PvuII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI